Restriction map for
pFN21AB8635
(Circular) (Six-base) MAPSORT of: pFN21AB8635.seq Check: 1786 from: 1 to: 5428
pFN21AB8635 5428 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2227 2227
Size: 5428
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3491 4875 5143 1632
Size: 1859 1384 268 1917
Fragments arranged by size:
1917 1859 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 2109 4327 5304 1203
Size: 906 2218 977 1327
Fragments arranged by size:
2218 1327 977 906
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 2425 1166
Size: 1259 4169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 2091 3171 4743 2091
Size: 1080 1572 2776
Fragments arranged by size:
2776 1572 1080
ApaI G_GGCC'C
Cuts at: 2192 2192
Size: 5428
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2737 2963 3115 5224 1493
Size: 1244 226 152 2109 1697
Fragments arranged by size:
2109 1697 1244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2389 2467 2753 1870
Size: 73 446 78 286 4545
Fragments arranged by size:
4545 446 286 78 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2374 2749 4204 618
Size: 325 217 627 587 375 1455 1842
Fragments arranged by size:
1842 1455 627 587 375 325 217
BanII G_rGCy'C
Cuts at: 1391 2192 2587 2747 1391
Size: 801 395 160 4072
Fragments arranged by size:
4072 801 395 160
BbeI G_GCGC'C
Cuts at: 2378 2378
Size: 5428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2370 2404 3619 3653 1903
Size: 34 50 34 349 34 1215 34 3678
Fragments arranged by size:
3678 1215 349 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4039 136
Size: 107 122 71 631 605 2367 1525
Fragments arranged by size:
2367 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2845 2845
Size: 5428
Bme1580I G_kGCm'C
Cuts at: 1790 2192 3489 4645 1790
Size: 402 1297 1156 2573
Fragments arranged by size:
2573 1297 1156 402
BmgBI CAC'GTC
Cuts at: 1318 2110 1318
Size: 792 4636
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3159 4117 442
Size: 416 53 83 399 24 1742 958 1753
Fragments arranged by size:
1753 1742 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2209 2562 4088 1239
Size: 911 59 353 1526 2579
Fragments arranged by size:
2579 1526 911 353 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2246 3553 4418 4716 2246
Size: 1307 865 298 2958
Fragments arranged by size:
2958 1307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2531 4091 882
Size: 617 1032 1560 2219
Fragments arranged by size:
2219 1560 1032 617
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2375 3235 3617 4252
Size: 53 83 186 936 842 860 382 635
Cuts at: 4252 275
Size: 1451
Fragments arranged by size:
1451 936 860 842 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3861 4259 4533 4680 5091 3861
Size: 398 274 147 411 4198
Fragments arranged by size:
4198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 2574 2604 1178
Size: 30 165 30 630 30 511 30 4002
Fragments arranged by size:
4002 630 511 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 2627 2649 1186
Size: 1126 315 22 3965
Fragments arranged by size:
3965 1126 315 22
BseYI C'CCAG_C
Cuts at: 2016 2097 2531 4631 5127 2016
Size: 81 434 2100 496 2317
Fragments arranged by size:
2317 2100 496 434 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2024 2126 2255 2263 2364 1163
Size: 573 288 102 129 8 101 4227
Fragments arranged by size:
4227 573 288 129 102 101 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2403 2878 3639 4667 1540
Size: 275 186 402 475 761 1028 2301
Fragments arranged by size:
2301 1028 761 475 402 275 186
BsiHKAI G_wGCw'C
Cuts at: 3489 3574 4645 3489
Size: 85 1071 4272
Fragments arranged by size:
4272 1071 85
BsmI GAATG_Cn'
Cuts at: 2939 3032 2939
Size: 93 5335
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2587 2747 3489 3574 4645 1391
Size: 399 402 395 160 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 395 160 85
BspEI T'CCGG_A
Cuts at: 5091 5091
Size: 5428
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 2227 2716 2783 844
Size: 1255 128 489 67 3489
Fragments arranged by size:
3489 1255 489 128 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2657 3315 1394
Size: 429 834 658 3507
Fragments arranged by size:
3507 834 658 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3923 4097 65
Size: 1183 2675 174 1396
Fragments arranged by size:
2675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2292 2545 4072 4259 1700
Size: 110 482 253 1527 187 2869
Fragments arranged by size:
2869 1527 482 253 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 2495 1925
Size: 570 4858
BssSI C'ACGA_G
Cuts at: 2297 3488 4500 2297
Size: 1191 1012 3225
Fragments arranged by size:
3225 1191 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5428
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2758 3125 3510 3527 4979 5065 5077 5423
Size: 786 367 385 17 1452 86 12 346
Cuts at: 5423 1972
Size: 1977
Fragments arranged by size:
1977 1452 786 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2360 513
Size: 552 1295 3581
Fragments arranged by size:
3581 1295 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3738 3758 2944
Size: 794 20 4614
Fragments arranged by size:
4614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DraIII CAC_nnn'GTG
Cuts at: 2110 2110
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4435 809
Size: 507 3119 1802
Fragments arranged by size:
3119 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2875 3764 5393
Size: 54 1425 50 275 18 1045 889 1629
Cuts at: 5393 8
Size: 43
Fragments arranged by size:
1629 1425 1045 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2069 3358 1353
Size: 698 18 1289 3423
Fragments arranged by size:
3423 1289 698 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2537 2726 4015 4389 4535 387
Size: 1064 138 948 189 1289 374 146 1280
Fragments arranged by size:
1289 1280 1064 948 374 189 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2209 2562 3491 4088 4875 5143
Size: 393 518 59 353 929 597 787 268
Cuts at: 5143 1239
Size: 1524
Fragments arranged by size:
1524 929 787 597 518 393 353 268
59
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 5428
EcoO109I rG'GnC_Cy
Cuts at: 2088 2119 2435 2088
Size: 31 316 5081
Fragments arranged by size:
5081 316 31
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2287 2194
Size: 93 5335
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 2378 4575 1678
Size: 700 2197 2531
Fragments arranged by size:
2531 2197 700
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 4069 4101 4143
Size: 32 135 32 878 32 1587 32 42
Cuts at: 4143 4175 1373
Size: 32 2626
Fragments arranged by size:
2626 1587 878 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2772 3018 669
Size: 988 281 834 246 3079
Fragments arranged by size:
3079 988 834 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5428
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KasI G'GCGC_C
Cuts at: 2374 2374
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2389 4542 4726 1305
Size: 1084 2153 184 2007
Fragments arranged by size:
2153 2007 1084 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3606
Fragments arranged by size:
3606 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2425 3386 3745 3904 518
Size: 648 1259 961 359 159 2042
Fragments arranged by size:
2042 1259 961 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2844 3521 4669 4914 1606
Size: 1238 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 677 245
NaeI GCC'GGC
Cuts at: 2294 2547 2294
Size: 253 5175
NarI GG'CG_CC
Cuts at: 2375 2375
Size: 5428
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4876
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NgoMIV G'CCGG_C
Cuts at: 2292 2545 2292
Size: 253 5175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2786 4331 1132
Size: 97 1557 1545 2229
Fragments arranged by size:
2229 1557 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2217 1108
Size: 1109 4319
PfoI T'CCnGG_A
Cuts at: 2551 2578 2551
Size: 27 5401
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2088 2119 2435 2088
Size: 31 316 5081
Fragments arranged by size:
5081 316 31
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PspOMI G'GGCC_C
Cuts at: 2188 2188
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2291 2780 830
Size: 1461 489 3478
Fragments arranged by size:
3478 1461 489
PvuI CG_AT'CG
Cuts at: 2001 2403 2001
Size: 402 5026
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SapI GCTCTTCn'nnn_
Cuts at: 2069 2069
Size: 5428
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SfcI C'TryA_G
Cuts at: 826 1045 2287 2776 3911 4592 4783 826
Size: 219 1242 489 1135 681 191 1471
Fragments arranged by size:
1471 1242 1135 681 489 219 191
SfoI GGC'GCC
Cuts at: 2376 2376
Size: 5428
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 3532 4433 4695 820
Size: 197 926 318 1271 901 262 1553
Fragments arranged by size:
1553 1271 926 901 318 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2867 513
Size: 552 1802 3074
Fragments arranged by size:
3074 1802 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2428 3455 3472 3625 3810 4255 325
Size: 1255 848 1027 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1027 848 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3674 5386 96
Size: 274 80 33 51 1443 1697 1712 138
Fragments arranged by size:
1712 1697 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2033 2065 2135 2167 2574 2606 5388 5420 2033
Size: 32 70 32 407 32 2782 32 2041
Fragments arranged by size:
2782 2041 407 70 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI XcmI