Restriction map for
pFN21AB8634
(Circular) (Six-base) MAPSORT of: pFN21AB8634.seq Check: 3692 from: 1 to: 5728
pFN21AB8634 5728 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2357 3071 1937
Size: 420 714 4594
Fragments arranged by size:
4594 714 420
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2166 3791 5175 5443 1632
Size: 534 1625 1384 268 1917
Fragments arranged by size:
1917 1625 1384 534 268
AfeI AGC'GCT
Cuts at: 2972 2972
Size: 5728
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 4627 5604 1203
Size: 3424 977 1327
Fragments arranged by size:
3424 1327 977
AgeI A'CCGG_T
Cuts at: 2520 4559 2520
Size: 2039 3689
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 2582 1166
Size: 1416 4312
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2284 2316 2284
Size: 32 5696
AlwNI CAG_nnn'CTG
Cuts at: 2304 2725 3471 5043 2304
Size: 421 746 1572 2989
Fragments arranged by size:
2989 1572 746 421
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 2171 3037 3263 3415 5524 1493
Size: 678 866 226 152 2109 1697
Fragments arranged by size:
2109 1697 866 678 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 3053 1870
Size: 73 250 860 4545
Fragments arranged by size:
4545 860 250 73
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3049 4504 618
Size: 325 217 627 1262 1455 1842
Fragments arranged by size:
1842 1455 1262 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2883 2902 3047 1391
Size: 1492 19 145 4072
Fragments arranged by size:
4072 1492 145 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3919 3953 1903
Size: 34 1982 34 3678
Fragments arranged by size:
3678 1982 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4339 136
Size: 107 122 71 631 605 2667 1525
Fragments arranged by size:
2667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5723 5723
Size: 5728
BlpI GC'TnA_GC
Cuts at: 2550 2974 3145 2550
Size: 424 171 5133
Fragments arranged by size:
5133 424 171
Bme1580I G_kGCm'C
Cuts at: 1790 3789 4945 1790
Size: 1999 1156 2573
Fragments arranged by size:
2573 1999 1156
BmgBI CAC'GTC
Cuts at: 1318 2233 1318
Size: 915 4813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3459 4417 442
Size: 416 53 83 399 24 2042 958 1753
Fragments arranged by size:
2042 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2259 4388 1239
Size: 854 166 2129 2579
Fragments arranged by size:
2579 2129 854 166
Bpu10I CC'TnA_GC
Cuts at: 2099 2864 2885 2099
Size: 765 21 4942
Fragments arranged by size:
4942 765 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2930 3853 4718 5016 2336
Size: 594 923 865 298 3048
Fragments arranged by size:
3048 923 865 594 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4391 882
Size: 617 2892 2219
Fragments arranged by size:
2892 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3519 493
Size: 711 2315 2702
Fragments arranged by size:
2702 2315 711
BsaBI GATnn'nnATC
Cuts at: 2445 2621 5722 2445
Size: 176 3101 2451
Fragments arranged by size:
3101 2451 176
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3535 3917 4552 275
Size: 53 83 186 936 2002 382 635 1451
Fragments arranged by size:
2002 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2049 2520 4161 4559 4833 4980 5391 2049
Size: 471 1641 398 274 147 411 2386
Fragments arranged by size:
2386 1641 471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5503
Fragments arranged by size:
5503 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5728
BseYI C'CCAG_C
Cuts at: 3013 4931 5427 3013
Size: 1918 496 3314
Fragments arranged by size:
3314 1918 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3003 1163
Size: 573 1267 3888
Fragments arranged by size:
3888 1267 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2143 3178 3939 4967 1540
Size: 275 186 142 1035 761 1028 2301
Fragments arranged by size:
2301 1035 1028 761 275 186 142
BsiHKAI G_wGCw'C
Cuts at: 3789 3874 4945 3789
Size: 85 1071 4572
Fragments arranged by size:
4572 1071 85
BsmI GAATG_Cn'
Cuts at: 2062 3239 3332 2062
Size: 1177 93 4458
Fragments arranged by size:
4458 1177 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2883 2902 3047 3789 3874 4945 1391
Size: 399 1093 19 145 742 85 1071 2174
Fragments arranged by size:
2174 1093 1071 742 399 145 85 19
BspEI T'CCGG_A
Cuts at: 2049 5391 2049
Size: 3342 2386
BspHI T'CATG_A
Cuts at: 3617 5347 3617
Size: 1730 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 3083 844
Size: 1289 950 3489
Fragments arranged by size:
3489 1289 950
BsrBI CCG'CTC
Cuts at: 1394 1823 3615 1394
Size: 429 1792 3507
Fragments arranged by size:
3507 1792 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4223 4397 65
Size: 1183 2975 174 1396
Fragments arranged by size:
2975 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 2520 4372 4559 1700
Size: 110 272 438 1852 187 2869
Fragments arranged by size:
2869 1852 438 272 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 2020 3788 4800 2020
Size: 1768 1012 2948
Fragments arranged by size:
2948 1768 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5728
BstBI TT'CG_AA
Cuts at: 2462 4532 2462
Size: 2070 3658
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstYI r'GATC_y
Cuts at: 1972 2721 2957 3058 3425 3810 3827 5279 5365
Size: 749 236 101 367 385 17 1452 86
Cuts at: 5365 5377 5723 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 749 385 367 346 236 101
86 17 12
BstZ17I GTA'TAC
Cuts at: 2358 2358
Size: 5728
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4237
Fragments arranged by size:
4237 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4989
Fragments arranged by size:
4989 683 56
BtsI GCAGTG_nn'
Cuts at: 3244 4038 4058 3244
Size: 794 20 4914
Fragments arranged by size:
4914 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2084 2631 3175
Size: 54 1425 50 275 18 254 547 544
Cuts at: 3175 4064 5693 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 547 544 275 254 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 3658 1353
Size: 908 1397 3423
Fragments arranged by size:
3423 1397 908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2166 2259 3791 4388 5175 5443
Size: 393 461 73 93 1532 597 787 268
Cuts at: 5443 1239
Size: 1524
Fragments arranged by size:
1532 1524 787 597 461 393 268 93
73
EcoNI CCTnn'n_nnAGG
Cuts at: 2930 2930
Size: 5728
EcoO109I rG'GnC_Cy
Cuts at: 2407 2947 2407
Size: 540 5188
EcoRI G'AATT_C
Cuts at: 2171 3037 3415 2171
Size: 866 378 4484
Fragments arranged by size:
4484 866 378
EcoRV GAT'ATC
Cuts at: 2623 2623
Size: 5728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 5728
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 2974 4875 1678
Size: 1296 1901 2531
Fragments arranged by size:
2531 1901 1296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4369 4401 4443 4475 1373
Size: 32 135 32 2797 32 42 32 2626
Fragments arranged by size:
2797 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2342 3072 3318 669
Size: 988 281 404 730 246 3079
Fragments arranged by size:
3079 988 730 404 281 246
HindIII A'AGCT_T
Cuts at: 748 2906 748
Size: 2158 3570
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4842 5026 1305
Size: 3537 184 2007
Fragments arranged by size:
3537 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3906
Fragments arranged by size:
3906 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 2738 3686 4045 4204 518
Size: 648 1416 156 948 359 159 2042
Fragments arranged by size:
2042 1416 948 648 359 159 156
MspA1I CmG'CkG
Cuts at: 1606 2554 3144 3821 4969 5214 1606
Size: 948 590 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 677 590 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4237
Fragments arranged by size:
4237 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NspI r_CATG'y
Cuts at: 1132 1229 3086 4631 1132
Size: 97 1857 1545 2229
Fragments arranged by size:
2229 1857 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5728
PciI A'CATG_T
Cuts at: 4627 4627
Size: 5728
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2682 1108
Size: 1574 4154
PfoI T'CCnGG_A
Cuts at: 2275 2275
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2003 2035 3797 3829 5310 5342 2003
Size: 32 1762 32 1481 32 2389
Fragments arranged by size:
2389 1762 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2407 2947 2407
Size: 540 5188
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PstI C_TGCA'G
Cuts at: 830 2130 2787 3080 830
Size: 1300 657 293 3478
Fragments arranged by size:
3478 1300 657 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
PvuII CAG'CTG
Cuts at: 2554 2554
Size: 5728
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SanDI GG'GwC_CC
Cuts at: 2407 2407
Size: 5728
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SfcI C'TryA_G
Cuts at: 826 1045 2126 2783 3076 4211 4892 5083 826
Size: 219 1081 657 293 1135 681 191 1471
Fragments arranged by size:
1471 1135 1081 681 657 293 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 3055 1872
Size: 1183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 2909 3832 4733 4995 820
Size: 197 926 408 558 923 901 262 1553
Fragments arranged by size:
1553 926 923 901 558 408 262 197
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 3086 1132
Size: 1954 3774
SspI AAT'ATT
Cuts at: 5 52 2208 3652 5
Size: 47 2156 1444 2081
Fragments arranged by size:
2156 2081 1444 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 2004 2676 2874 3167 513
Size: 552 939 672 198 293 3074
Fragments arranged by size:
3074 939 672 552 293 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3755 3772 3925 4110 4555 325
Size: 1255 2175 17 153 185 445 1498
Fragments arranged by size:
2175 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2940 3974 5686
Size: 274 80 33 51 1443 963 1034 1712
Cuts at: 5686 96
Size: 138
Fragments arranged by size:
1712 1443 1034 963 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5688 5720 5688
Size: 32 5696
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5728
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2578 2578
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5728
XmaI C'CCGG_G
Cuts at: 1870 3053 1870
Size: 1183 4545
XmnI GAAnn'nnTTC
Cuts at: 2448 3857 2448
Size: 1409 4319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI