Restriction map for
pFN21AB8633
(Circular) (Six-base) MAPSORT of: pFN21AB8633.seq Check: 5408 from: 1 to: 6211
pFN21AB8633 6211 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3554 1937
Size: 1617 4594
Acc65I G'GTAC_C
Cuts at: 3178 3532 3178
Size: 354 5857
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 4274 5658 5926 1632
Size: 824 1818 1384 268 1917
Fragments arranged by size:
1917 1818 1384 824 268
AfeI AGC'GCT
Cuts at: 2945 2945
Size: 6211
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 2157 5110 6087 1203
Size: 954 2953 977 1327
Fragments arranged by size:
2953 1327 977 954
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2442 1184
Size: 1258 4953
AlwNI CAG_nnn'CTG
Cuts at: 3107 3954 5526 3107
Size: 847 1572 3792
Fragments arranged by size:
3792 1572 847
ApaLI G'TGCA_C
Cuts at: 4268 5424 4268
Size: 1156 5055
ApoI r'AATT_y
Cuts at: 1493 2546 3520 3746 3898 6007 1493
Size: 1053 974 226 152 2109 1697
Fragments arranged by size:
2109 1697 1053 974 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 3536 1870
Size: 73 1593 4545
Fragments arranged by size:
4545 1593 73
BamHI G'GATC_C
Cuts at: 3541 3908 3541
Size: 367 5844
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2956 3178 3288 3532
Size: 325 217 627 224 945 222 110 244
Cuts at: 3532 4987 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 945 627 325 244 224 222
217 110
BanII G_rGCy'C
Cuts at: 1391 2847 3038 3214 3530 1391
Size: 1456 191 176 316 4072
Fragments arranged by size:
4072 1456 316 191 176
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 6211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2642 2643 928
Size: 971 743 1 4496
Fragments arranged by size:
4496 971 743 1
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4402 4436 1903
Size: 34 2465 34 3678
Fragments arranged by size:
3678 2465 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 2680 1548
Size: 1132 5079
BfrBI ATG'CAT
Cuts at: 2891 2891
Size: 6211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4822 136
Size: 107 122 71 631 605 337 2813 1525
Fragments arranged by size:
2813 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6206 6206
Size: 6211
BlpI GC'TnA_GC
Cuts at: 2430 3628 2430
Size: 1198 5013
Bme1580I G_kGCm'C
Cuts at: 1790 3293 4272 5428 1790
Size: 1503 979 1156 2573
Fragments arranged by size:
2573 1503 1156 979
BmgBI CAC'GTC
Cuts at: 1318 2160 2740 1318
Size: 842 580 4789
Fragments arranged by size:
4789 842 580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2167 3128 3942
Size: 416 53 83 399 24 750 961 814
Cuts at: 3942 4900 442
Size: 958 1753
Fragments arranged by size:
1753 961 958 814 750 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3114 1056
Size: 2058 4153
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3233
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 3194 3211 4871 1239
Size: 1331 624 17 1660 2579
Fragments arranged by size:
2579 1660 1331 624 17
Bpu10I CC'TnA_GC
Cuts at: 3096 3096
Size: 6211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2948 4336 5201 5499 2333
Size: 615 1388 865 298 3045
Fragments arranged by size:
3045 1388 865 615 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4874 882
Size: 617 3375 2219
Fragments arranged by size:
3375 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4002 493
Size: 711 2798 2702
Fragments arranged by size:
2798 2702 711
BsaBI GATnn'nnATC
Cuts at: 6205 6205
Size: 6211
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 4018 4400 5035
Size: 53 83 186 936 479 2006 382 635
Cuts at: 5035 275
Size: 1451
Fragments arranged by size:
2006 1451 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4644 5042 5316 5463 5874 4644
Size: 398 274 147 411 4981
Fragments arranged by size:
4981 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5986
Fragments arranged by size:
5986 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2958 3214 1186
Size: 1772 256 4183
Fragments arranged by size:
4183 1772 256
BseYI C'CCAG_C
Cuts at: 2322 2769 3066 3361 5414 5910 2322
Size: 447 297 295 2053 496 2623
Fragments arranged by size:
2623 2053 496 447 297 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2082 1163
Size: 573 346 5292
Fragments arranged by size:
5292 573 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2357 3214 3506 4272 4357 5428 2357
Size: 857 292 766 85 1071 3140
Fragments arranged by size:
3140 1071 857 766 292 85
BsmI GAATG_Cn'
Cuts at: 3722 3815 3722
Size: 93 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2357 2847 3038 3214 3293 3506 3530
Size: 399 567 490 191 176 79 213 24
Cuts at: 3530 4272 4357 5428 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 490 399 213 191
176 85 79 24
BspEI T'CCGG_A
Cuts at: 5874 5874
Size: 6211
BspHI T'CATG_A
Cuts at: 4100 5830 4100
Size: 1730 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2267 3566 844
Size: 1423 1299 3489
Fragments arranged by size:
3489 1423 1299
BsrBI CCG'CTC
Cuts at: 1394 1823 4098 1394
Size: 429 2275 3507
Fragments arranged by size:
3507 2275 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2831 4706 4880 65
Size: 1183 1583 1875 174 1396
Fragments arranged by size:
1875 1583 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4855 5042 1700
Size: 110 3045 187 2869
Fragments arranged by size:
3045 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 4271 5283 4271
Size: 1012 5199
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6211
BstBI TT'CG_AA
Cuts at: 5015 5015
Size: 6211
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6211
BstXI CCAn_nnnn'nTGG
Cuts at: 3400 3400
Size: 6211
BstYI r'GATC_y
Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206
Size: 1569 367 385 17 1452 86 12 346
Cuts at: 6206 1972
Size: 1977
Fragments arranged by size:
1977 1569 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2600 2600
Size: 6211
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4720
Fragments arranged by size:
4720 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5472
Fragments arranged by size:
5472 683 56
BtsI GCAGTG_nn'
Cuts at: 2742 3390 3494 3727 4521 4541 2742
Size: 648 104 233 794 20 4412
Fragments arranged by size:
4412 794 648 233 104 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6176
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2732 5218 809
Size: 507 1416 2486 1802
Fragments arranged by size:
2486 1802 1416 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2477 3658 4547
Size: 54 1425 50 275 18 647 1181 889
Cuts at: 4547 6176 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1181 889 647 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 3079 4141 1353
Size: 774 952 1062 3423
Fragments arranged by size:
3423 1062 952 774
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4798 5172 5318 387
Size: 1064 138 3209 374 146 1280
Fragments arranged by size:
3209 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3212 3212
Size: 6211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 2570 3194 3211 4274 4871 5658
Size: 393 824 114 624 17 1063 597 787
Cuts at: 5658 5926 1239
Size: 268 1524
Fragments arranged by size:
1524 1063 824 787 624 597 393 268
114 17
EcoO109I rG'GnC_Cy
Cuts at: 2085 2085
Size: 6211
EcoRI G'AATT_C
Cuts at: 2546 3520 3898 2546
Size: 974 378 4859
Fragments arranged by size:
4859 974 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2693 2461
Size: 232 5979
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 2015 2947 3360 5358 1678
Size: 337 932 413 1998 2531
Fragments arranged by size:
2531 1998 932 413 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 3025 3057 3167
Size: 32 135 32 902 32 519 32 110
Cuts at: 3167 3199 3254 3286 4852 4884 4926 4958 1373
Size: 32 55 32 1566 32 42 32 2626
Fragments arranged by size:
2626 1566 902 519 135 110 55 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3088 3555 3801 669
Size: 988 281 1150 467 246 3079
Fragments arranged by size:
3079 1150 988 467 281 246
HindIII A'AGCT_T
Cuts at: 748 2367 3253 748
Size: 1619 886 3706
Fragments arranged by size:
3706 1619 886
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 6211
KpnI G_GTAC'C
Cuts at: 3182 3536 3182
Size: 354 5857
MfeI C'AATT_G
Cuts at: 3810 3810
Size: 6211
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5325 5509 1305
Size: 4020 184 2007
Fragments arranged by size:
4020 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2479 10
Size: 54 1425 343 647 3742
Fragments arranged by size:
3742 1425 647 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4169 4528 4687 518
Size: 648 3003 359 159 2042
Fragments arranged by size:
3003 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 3428 3627 4304 5452 5697 1606
Size: 501 1321 199 677 1148 245 2120
Fragments arranged by size:
2120 1321 1148 677 501 245 199
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 6211
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4720
Fragments arranged by size:
4720 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6211
NheI G'CTAG_C
Cuts at: 1052 3110 1052
Size: 2058 4153
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NsiI A_TGCA'T
Cuts at: 2893 2893
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 2820 3569 5114 1132
Size: 97 1591 749 1545 2229
Fragments arranged by size:
2229 1591 1545 749 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6211
PciI A'CATG_T
Cuts at: 5110 5110
Size: 6211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6211
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5793 5825 4280
Size: 32 1481 32 4666
Fragments arranged by size:
4666 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2085 2085
Size: 6211
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PstI C_TGCA'G
Cuts at: 830 3563 830
Size: 2733 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 2107 3428 2107
Size: 1321 4890
SacI G_AGCT'C
Cuts at: 3214 3214
Size: 6211
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SanDI GG'GwC_CC
Cuts at: 2085 2085
Size: 6211
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 4459 6171 4459
Size: 1712 4499
SfcI C'TryA_G
Cuts at: 826 1045 3559 4694 5375 5566 826
Size: 219 2514 1135 681 191 1471
Fragments arranged by size:
2514 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 6211
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 3538 1872
Size: 1666 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 2927 4315 5216 5478 820
Size: 197 926 405 579 1388 901 262 1553
Fragments arranged by size:
1553 1388 926 901 579 405 262 197
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 3480 4135 5
Size: 47 3428 655 2081
Fragments arranged by size:
3428 2081 655 47
StuI AGG'CCT
Cuts at: 1517 2925 1517
Size: 1408 4803
StyI C'CwwG_G
Cuts at: 513 1065 2004 3293 3650 513
Size: 552 939 1289 357 3074
Fragments arranged by size:
3074 1289 939 552 357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2514 3286 4238 4255 4408 4593 5038
Size: 1255 934 772 952 17 153 185 445
Cuts at: 5038 325
Size: 1498
Fragments arranged by size:
1498 1255 952 934 772 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2450 4457 6169
Size: 274 80 33 51 1443 473 2007 1712
Cuts at: 6169 96
Size: 138
Fragments arranged by size:
2007 1712 1443 473 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3485 3517 6171 6203 3485
Size: 32 2654 32 3493
Fragments arranged by size:
3493 2654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6211
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2180 2881 2180
Size: 701 5510
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6211
XmaI C'CCGG_G
Cuts at: 1870 3536 1870
Size: 1666 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 4340 3470
Size: 870 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SapI SexAI SfiI SrfI SwaI