Restriction map for
pFN21AB8630
(Circular) (Six-base) MAPSORT of: pFN21AB8630.seq Check: 800 from: 1 to: 7213
pFN21AB8630 7213 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5023 278
Size: 53 83 186 936 3487 2468
Fragments arranged by size:
3487 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3003 3888 4556 1937
Size: 1066 885 668 4594
Fragments arranged by size:
4594 1066 885 668
Acc65I G'GTAC_C
Cuts at: 4534 4534
Size: 7213
AclI AA'CG_TT
Cuts at: 1563 5340 5713 1563
Size: 3777 373 3063
Fragments arranged by size:
3777 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3533 3555 5276 6660 6928 1632
Size: 1901 22 1721 1384 268 1917
Fragments arranged by size:
1917 1901 1721 1384 268 22
AfeI AGC'GCT
Cuts at: 2029 2029
Size: 7213
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7016
AflIII A'CryG_T
Cuts at: 1203 6112 7089 1203
Size: 4909 977 1327
Fragments arranged by size:
4909 1327 977
AgeI A'CCGG_T
Cuts at: 3704 6044 3704
Size: 2340 4873
AhdI GACnn_n'nnGTC
Cuts at: 5942 5942
Size: 7213
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2562 2594 3148 3180 2562
Size: 32 554 32 6595
Fragments arranged by size:
6595 554 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2300 2508 4956 6528 2300
Size: 208 2448 1572 2985
Fragments arranged by size:
2985 2448 1572 208
ApaI G_GGCC'C
Cuts at: 4420 4420
Size: 7213
ApaLI G'TGCA_C
Cuts at: 5270 6426 5270
Size: 1156 6057
ApoI r'AATT_y
Cuts at: 1493 3528 4522 4748 4900 7009 1493
Size: 2035 994 226 152 2109 1697
Fragments arranged by size:
2109 2035 1697 994 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5767 160
Size: 872 4735 1606
Fragments arranged by size:
4735 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
AvaI C'yCGr_G
Cuts at: 1870 1943 2995 4538 1870
Size: 73 1052 1543 4545
Fragments arranged by size:
4545 1543 1052 73
AvrII C'CTAG_G
Cuts at: 3838 3838
Size: 7213
BamHI G'GATC_C
Cuts at: 4123 4543 4910 4123
Size: 420 367 6426
Fragments arranged by size:
6426 420 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2723 4534 5989 618
Size: 325 217 627 936 1811 1455 1842
Fragments arranged by size:
1842 1811 1455 936 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2996 3328 3815 4420 4532 1391
Size: 649 956 332 487 605 112 4072
Fragments arranged by size:
4072 956 649 605 487 332 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4240 928
Size: 971 2341 3901
Fragments arranged by size:
3901 2341 971
BbvCI CC'TCA_GC
Cuts at: 4325 4325
Size: 7213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5404 5438 1903
Size: 34 3467 34 3678
Fragments arranged by size:
3678 3467 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2336 5105 6315 2336
Size: 2769 1210 3234
Fragments arranged by size:
3234 2769 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7213
BfrBI ATG'CAT
Cuts at: 4150 4150
Size: 7213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5824 136
Size: 107 122 71 631 605 4152 1525
Fragments arranged by size:
4152 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7208 7208
Size: 7213
BlpI GC'TnA_GC
Cuts at: 4630 4630
Size: 7213
Bme1580I G_kGCm'C
Cuts at: 1790 2728 4420 5274 6430 1790
Size: 938 1692 854 1156 2573
Fragments arranged by size:
2573 1692 1156 938 854
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4944 5902 442
Size: 416 53 83 399 24 3527 958 1753
Fragments arranged by size:
3527 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4067 4281 1056
Size: 3011 214 3988
Fragments arranged by size:
3988 3011 214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2046 2046
Size: 7213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2278 2947 3599 4373 5873 1239
Size: 1039 669 652 774 1500 2579
Fragments arranged by size:
2579 1500 1039 774 669 652
Bpu10I CC'TnA_GC
Cuts at: 2681 4325 2681
Size: 1644 5569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3341 3392 4412 5338 6203 6501 3341
Size: 51 1020 926 865 298 4053
Fragments arranged by size:
4053 1020 926 865 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2309 5876 882
Size: 617 810 3567 2219
Fragments arranged by size:
3567 2219 810 617
BsaAI yAC'GTr
Cuts at: 493 1204 5004 493
Size: 711 3800 2702
Fragments arranged by size:
3800 2702 711
BsaBI GATnn'nnATC
Cuts at: 7207 7207
Size: 7213
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5020 5402 6037 275
Size: 53 83 186 936 3487 382 635 1451
Fragments arranged by size:
3487 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3704 5646 6044 6318 6465 6876 3704
Size: 1942 398 274 147 411 4041
Fragments arranged by size:
4041 1942 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2562 2592 2952 2982 1178
Size: 30 165 30 1159 30 360 30 5409
Fragments arranged by size:
5409 1159 360 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 2792 1186
Size: 1255 351 5607
Fragments arranged by size:
5607 1255 351
BseYI C'CCAG_C
Cuts at: 3159 3478 6416 6912 3159
Size: 319 2938 496 3460
Fragments arranged by size:
3460 2938 496 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2194 4355 1163
Size: 573 458 2161 4021
Fragments arranged by size:
4021 2161 573 458
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4663 5424 6452 1540
Size: 275 186 2662 761 1028 2301
Fragments arranged by size:
2662 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3815 5274 5359 6430 3815
Size: 1459 85 1071 4598
Fragments arranged by size:
4598 1459 1071 85
BsmI GAATG_Cn'
Cuts at: 3017 3052 4724 4817 3017
Size: 35 1672 93 5413
Fragments arranged by size:
5413 1672 93 35
BsmBI CGTCTCn'nnnn_
Cuts at: 4187 4187
Size: 7213
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2728 2996 3328 3815 4420 4532
Size: 399 250 688 268 332 487 605 112
Cuts at: 4532 5274 5359 6430 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 688 605 487 399 332
268 250 112 85
BspEI T'CCGG_A
Cuts at: 6876 6876
Size: 7213
BspHI T'CATG_A
Cuts at: 3853 5102 6832 3853
Size: 1249 1730 4234
Fragments arranged by size:
4234 1730 1249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3618 4419 4568 844
Size: 2774 801 149 3489
Fragments arranged by size:
3489 2774 801 149
BsrBI CCG'CTC
Cuts at: 1394 1823 5100 1394
Size: 429 3277 3507
Fragments arranged by size:
3507 3277 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3022 5708 5882 65
Size: 1183 1774 2686 174 1396
Fragments arranged by size:
2686 1774 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2199 3704 5857 6044 1700
Size: 110 389 1505 2153 187 2869
Fragments arranged by size:
2869 2153 1505 389 187 110
BsrGI T'GTAC_A
Cuts at: 96 3831 3939 96
Size: 3735 108 3370
Fragments arranged by size:
3735 3370 108
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7213
BssSI C'ACGA_G
Cuts at: 5273 6285 5273
Size: 1012 6201
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7213
BstBI TT'CG_AA
Cuts at: 6017 6017
Size: 7213
BstEII G'GTnAC_C
Cuts at: 1184 3494 1184
Size: 2310 4903
BstXI CCAn_nnnn'nTGG
Cuts at: 2240 2240
Size: 7213
BstYI r'GATC_y
Cuts at: 1972 2325 3771 4016 4123 4543 4910 5295 5312
Size: 353 1446 245 107 420 367 385 17
Cuts at: 5312 6764 6850 6862 7208 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1446 420 385 367 353 346
245 107 86 17 12
BstZ17I GTA'TAC
Cuts at: 3889 3889
Size: 7213
BtgI C'CryG_G
Cuts at: 513 1065 2129 513
Size: 552 1064 5597
Fragments arranged by size:
5597 1064 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6474
Fragments arranged by size:
6474 683 56
BtsI GCAGTG_nn'
Cuts at: 2406 4729 5523 5543 2406
Size: 2323 794 20 4076
Fragments arranged by size:
4076 2323 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7213
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3918 3953 529
Size: 35 3354 35 3789
Fragments arranged by size:
3789 3354 35 35
DraI TTT'AAA
Cuts at: 3699 4517 3699
Size: 818 6395
DraIII CAC_nnn'GTG
Cuts at: 2944 2944
Size: 7213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6220 809
Size: 507 4904 1802
Fragments arranged by size:
4904 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2201 3660 4660
Size: 54 1425 50 275 18 371 1459 1000
Cuts at: 4660 5549 7178 8
Size: 889 1629 43
Fragments arranged by size:
1629 1459 1425 1000 889 371 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4660 1537
Size: 275 2848 4090
Fragments arranged by size:
4090 2848 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2312 2447 2984 3509 4106 5143 1353
Size: 819 140 135 537 525 597 1037 3423
Fragments arranged by size:
3423 1037 819 597 537 525 140 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5800 6174 6320 387
Size: 1064 138 4211 374 146 1280
Fragments arranged by size:
4211 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3813 3813
Size: 7213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2278 2947 3533 3555 3599 4373 5276
Size: 393 646 669 586 22 44 774 903
Cuts at: 5276 5873 6660 6928 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 903 787 774 669 646 597 586
393 268 44 22
EcoO109I rG'GnC_Cy
Cuts at: 2110 3793 4417 2110
Size: 1683 624 4906
Fragments arranged by size:
4906 1683 624
EcoRI G'AATT_C
Cuts at: 4522 4900 4522
Size: 378 6835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3124 4120 3124
Size: 996 6217
FspI TGC'GCA
Cuts at: 2539 5719 2539
Size: 3180 4033
HaeII r_GCGC'y
Cuts at: 1678 2031 3549 6360 1678
Size: 353 1518 2811 2531
Fragments arranged by size:
2811 2531 1518 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2251 2283 2412 2444 2952
Size: 32 135 32 679 32 129 32 508
Cuts at: 2952 2984 3086 3118 5854 5886 5928 5960 1373
Size: 32 102 32 2736 32 42 32 2626
Fragments arranged by size:
2736 2626 679 508 135 129 102 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4557 4803 669
Size: 988 281 2619 246 3079
Fragments arranged by size:
3079 2619 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2314 2758 748
Size: 1566 444 5203
Fragments arranged by size:
5203 1566 444
HpaI GTT'AAC
Cuts at: 4803 4803
Size: 7213
KpnI G_GTAC'C
Cuts at: 4538 4538
Size: 7213
MfeI C'AATT_G
Cuts at: 4812 4812
Size: 7213
MluI A'CGCG_T
Cuts at: 7089 7089
Size: 7213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2790 6327 6511 1305
Size: 1485 3537 184 2007
Fragments arranged by size:
3537 2007 1485 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3662 10
Size: 54 1425 343 1830 3561
Fragments arranged by size:
3561 1830 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4377 5171 5530 5689 518
Size: 648 3211 794 359 159 2042
Fragments arranged by size:
3211 2042 794 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2783 4196 4217 4295 4629 5306 6454 6699
Size: 1177 1413 21 78 334 677 1148 245
Cuts at: 6699 1606
Size: 2120
Fragments arranged by size:
2120 1413 1177 1148 677 334 245 78
21
NcoI C'CATG_G
Cuts at: 513 1065 2129 513
Size: 552 1064 5597
Fragments arranged by size:
5597 1064 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7213
NheI G'CTAG_C
Cuts at: 1052 4063 4277 1052
Size: 3011 214 3988
Fragments arranged by size:
3988 3011 214
NotI GC'GGCC_GC
Cuts at: 4660 4660
Size: 7213
NsiI A_TGCA'T
Cuts at: 4152 4152
Size: 7213
NspI r_CATG'y
Cuts at: 1132 1229 2291 2554 3592 4150 4267 4571 6116
Size: 97 1062 263 1038 558 117 304 1545
Cuts at: 6116 1132
Size: 2229
Fragments arranged by size:
2229 1545 1062 1038 558 304 263 117
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7213
PciI A'CATG_T
Cuts at: 6112 6112
Size: 7213
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2508 1108
Size: 1400 5813
PfoI T'CCnGG_A
Cuts at: 3615 3615
Size: 7213
PmeI GTTT'AAAC
Cuts at: 4517 4517
Size: 7213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2562 2594 5282 5314 6795 6827 2562
Size: 32 2688 32 1481 32 2948
Fragments arranged by size:
2948 2688 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 3793 2110
Size: 1683 5530
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7213
PsiI TTA'TAA
Cuts at: 3597 3654 4783 3597
Size: 57 1129 6027
Fragments arranged by size:
6027 1129 57
PspOMI G'GGCC_C
Cuts at: 4416 4416
Size: 7213
PstI C_TGCA'G
Cuts at: 830 4425 4565 830
Size: 3595 140 3478
Fragments arranged by size:
3595 3478 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7213
PvuII CAG'CTG
Cuts at: 4196 4217 4295 4196
Size: 21 78 7114
Fragments arranged by size:
7114 78 21
SacI G_AGCT'C
Cuts at: 3815 3815
Size: 7213
SalI G'TCGA_C
Cuts at: 1936 4555 1936
Size: 2619 4594
SapI GCTCTTCn'nnn_
Cuts at: 2984 4106 2984
Size: 1122 6091
SbfI CC_TGCA'GG
Cuts at: 4565 4565
Size: 7213
ScaI AGT'ACT
Cuts at: 5461 7173 5461
Size: 1712 5501
SfcI C'TryA_G
Cuts at: 826 1045 3378 3550 4421 4561 5696 6377 6568
Size: 219 2333 172 871 140 1135 681 191
Cuts at: 6568 826
Size: 1471
Fragments arranged by size:
2333 1471 1135 871 681 219 191 172
140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7213
SmaI CCC'GGG
Cuts at: 1872 4540 1872
Size: 2668 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3356 3407 4427 5317 6218 6480
Size: 197 926 1413 51 1020 890 901 262
Cuts at: 6480 820
Size: 1553
Fragments arranged by size:
1553 1413 1020 926 901 890 262 197
51
SnaBI TAC'GTA
Cuts at: 493 5004 493
Size: 4511 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7213
SphI G_CATG'C
Cuts at: 1132 2291 4571 1132
Size: 1159 2280 3774
Fragments arranged by size:
3774 2280 1159
SspI AAT'ATT
Cuts at: 5 52 5137 5
Size: 47 5085 2081
Fragments arranged by size:
5085 2081 47
StuI AGG'CCT
Cuts at: 1517 3676 4330 1517
Size: 2159 654 4400
Fragments arranged by size:
4400 2159 654
StyI C'CwwG_G
Cuts at: 513 1065 2129 2332 3499 3724 3838 3914 4652
Size: 552 1064 203 1167 225 114 76 738
Cuts at: 4652 513
Size: 3074
Fragments arranged by size:
3074 1167 1064 738 552 225 203 114
76
SwaI ATTT'AAAT
Cuts at: 3699 3699
Size: 7213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2711 3305 3941 5240 5257 5410 5595
Size: 1255 1131 594 636 1299 17 153 185
Cuts at: 5595 6040 325
Size: 445 1498
Fragments arranged by size:
1498 1299 1255 1131 636 594 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3564 3831 3939
Size: 274 80 33 51 1443 1587 267 108
Cuts at: 3939 5459 7171 96
Size: 1520 1712 138
Fragments arranged by size:
1712 1587 1520 1443 274 267 138 108
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7173 7205 7173
Size: 32 7181
Tth111I GACn'n_nGTC
Cuts at: 1316 3100 4439 1316
Size: 1784 1339 4090
Fragments arranged by size:
4090 1784 1339
XbaI T'CTAG_A
Cuts at: 4549 4549
Size: 7213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2127 3486 2127
Size: 1359 5854
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7213
XmaI C'CCGG_G
Cuts at: 1870 4538 1870
Size: 2668 4545
XmnI GAAnn'nnTTC
Cuts at: 3900 5342 3900
Size: 1442 5771
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5021 276
Size: 53 83 186 936 3487 2468
Fragments arranged by size:
3487 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BsiWI Bsu36I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI