(Circular) (Six-base) MAPSORT of: pFN21AB8630.seq Check: 800 from: 1 to: 7213 pFN21AB8630 7213 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5023 278 Size: 53 83 186 936 3487 2468 Fragments arranged by size: 3487 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3003 3888 4556 1937 Size: 1066 885 668 4594 Fragments arranged by size: 4594 1066 885 668 Acc65I G'GTAC_C Cuts at: 4534 4534 Size: 7213 AclI AA'CG_TT Cuts at: 1563 5340 5713 1563 Size: 3777 373 3063 Fragments arranged by size: 3777 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3533 3555 5276 6660 6928 1632 Size: 1901 22 1721 1384 268 1917 Fragments arranged by size: 1917 1901 1721 1384 268 22 AfeI AGC'GCT Cuts at: 2029 2029 Size: 7213 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7016 AflIII A'CryG_T Cuts at: 1203 6112 7089 1203 Size: 4909 977 1327 Fragments arranged by size: 4909 1327 977 AgeI A'CCGG_T Cuts at: 3704 6044 3704 Size: 2340 4873 AhdI GACnn_n'nnGTC Cuts at: 5942 5942 Size: 7213 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7213 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7213 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2562 2594 3148 3180 2562 Size: 32 554 32 6595 Fragments arranged by size: 6595 554 32 32 AlwNI CAG_nnn'CTG Cuts at: 2300 2508 4956 6528 2300 Size: 208 2448 1572 2985 Fragments arranged by size: 2985 2448 1572 208 ApaI G_GGCC'C Cuts at: 4420 4420 Size: 7213 ApaLI G'TGCA_C Cuts at: 5270 6426 5270 Size: 1156 6057 ApoI r'AATT_y Cuts at: 1493 3528 4522 4748 4900 7009 1493 Size: 2035 994 226 152 2109 1697 Fragments arranged by size: 2109 2035 1697 994 226 152 AseI AT'TA_AT Cuts at: 160 1032 5767 160 Size: 872 4735 1606 Fragments arranged by size: 4735 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7213 AvaI C'yCGr_G Cuts at: 1870 1943 2995 4538 1870 Size: 73 1052 1543 4545 Fragments arranged by size: 4545 1543 1052 73 AvrII C'CTAG_G Cuts at: 3838 3838 Size: 7213 BamHI G'GATC_C Cuts at: 4123 4543 4910 4123 Size: 420 367 6426 Fragments arranged by size: 6426 420 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2723 4534 5989 618 Size: 325 217 627 936 1811 1455 1842 Fragments arranged by size: 1842 1811 1455 936 627 325 217 BanII G_rGCy'C Cuts at: 1391 2040 2996 3328 3815 4420 4532 1391 Size: 649 956 332 487 605 112 4072 Fragments arranged by size: 4072 956 649 605 487 332 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4240 928 Size: 971 2341 3901 Fragments arranged by size: 3901 2341 971 BbvCI CC'TCA_GC Cuts at: 4325 4325 Size: 7213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5404 5438 1903 Size: 34 3467 34 3678 Fragments arranged by size: 3678 3467 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2336 5105 6315 2336 Size: 2769 1210 3234 Fragments arranged by size: 3234 2769 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7213 BfrBI ATG'CAT Cuts at: 4150 4150 Size: 7213 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5824 136 Size: 107 122 71 631 605 4152 1525 Fragments arranged by size: 4152 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7208 7208 Size: 7213 BlpI GC'TnA_GC Cuts at: 4630 4630 Size: 7213 Bme1580I G_kGCm'C Cuts at: 1790 2728 4420 5274 6430 1790 Size: 938 1692 854 1156 2573 Fragments arranged by size: 2573 1692 1156 938 854 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4944 5902 442 Size: 416 53 83 399 24 3527 958 1753 Fragments arranged by size: 3527 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4067 4281 1056 Size: 3011 214 3988 Fragments arranged by size: 3988 3011 214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2046 2046 Size: 7213 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2278 2947 3599 4373 5873 1239 Size: 1039 669 652 774 1500 2579 Fragments arranged by size: 2579 1500 1039 774 669 652 Bpu10I CC'TnA_GC Cuts at: 2681 4325 2681 Size: 1644 5569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3341 3392 4412 5338 6203 6501 3341 Size: 51 1020 926 865 298 4053 Fragments arranged by size: 4053 1020 926 865 298 51 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2309 5876 882 Size: 617 810 3567 2219 Fragments arranged by size: 3567 2219 810 617 BsaAI yAC'GTr Cuts at: 493 1204 5004 493 Size: 711 3800 2702 Fragments arranged by size: 3800 2702 711 BsaBI GATnn'nnATC Cuts at: 7207 7207 Size: 7213 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5020 5402 6037 275 Size: 53 83 186 936 3487 382 635 1451 Fragments arranged by size: 3487 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3704 5646 6044 6318 6465 6876 3704 Size: 1942 398 274 147 411 4041 Fragments arranged by size: 4041 1942 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2562 2592 2952 2982 1178 Size: 30 165 30 1159 30 360 30 5409 Fragments arranged by size: 5409 1159 360 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 2792 1186 Size: 1255 351 5607 Fragments arranged by size: 5607 1255 351 BseYI C'CCAG_C Cuts at: 3159 3478 6416 6912 3159 Size: 319 2938 496 3460 Fragments arranged by size: 3460 2938 496 319 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2194 4355 1163 Size: 573 458 2161 4021 Fragments arranged by size: 4021 2161 573 458 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4663 5424 6452 1540 Size: 275 186 2662 761 1028 2301 Fragments arranged by size: 2662 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3815 5274 5359 6430 3815 Size: 1459 85 1071 4598 Fragments arranged by size: 4598 1459 1071 85 BsmI GAATG_Cn' Cuts at: 3017 3052 4724 4817 3017 Size: 35 1672 93 5413 Fragments arranged by size: 5413 1672 93 35 BsmBI CGTCTCn'nnnn_ Cuts at: 4187 4187 Size: 7213 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2728 2996 3328 3815 4420 4532 Size: 399 250 688 268 332 487 605 112 Cuts at: 4532 5274 5359 6430 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 688 605 487 399 332 268 250 112 85 BspEI T'CCGG_A Cuts at: 6876 6876 Size: 7213 BspHI T'CATG_A Cuts at: 3853 5102 6832 3853 Size: 1249 1730 4234 Fragments arranged by size: 4234 1730 1249 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3618 4419 4568 844 Size: 2774 801 149 3489 Fragments arranged by size: 3489 2774 801 149 BsrBI CCG'CTC Cuts at: 1394 1823 5100 1394 Size: 429 3277 3507 Fragments arranged by size: 3507 3277 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3022 5708 5882 65 Size: 1183 1774 2686 174 1396 Fragments arranged by size: 2686 1774 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2199 3704 5857 6044 1700 Size: 110 389 1505 2153 187 2869 Fragments arranged by size: 2869 2153 1505 389 187 110 BsrGI T'GTAC_A Cuts at: 96 3831 3939 96 Size: 3735 108 3370 Fragments arranged by size: 3735 3370 108 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7213 BssSI C'ACGA_G Cuts at: 5273 6285 5273 Size: 1012 6201 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7213 BstBI TT'CG_AA Cuts at: 6017 6017 Size: 7213 BstEII G'GTnAC_C Cuts at: 1184 3494 1184 Size: 2310 4903 BstXI CCAn_nnnn'nTGG Cuts at: 2240 2240 Size: 7213 BstYI r'GATC_y Cuts at: 1972 2325 3771 4016 4123 4543 4910 5295 5312 Size: 353 1446 245 107 420 367 385 17 Cuts at: 5312 6764 6850 6862 7208 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1446 420 385 367 353 346 245 107 86 17 12 BstZ17I GTA'TAC Cuts at: 3889 3889 Size: 7213 BtgI C'CryG_G Cuts at: 513 1065 2129 513 Size: 552 1064 5597 Fragments arranged by size: 5597 1064 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6474 Fragments arranged by size: 6474 683 56 BtsI GCAGTG_nn' Cuts at: 2406 4729 5523 5543 2406 Size: 2323 794 20 4076 Fragments arranged by size: 4076 2323 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7213 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3918 3953 529 Size: 35 3354 35 3789 Fragments arranged by size: 3789 3354 35 35 DraI TTT'AAA Cuts at: 3699 4517 3699 Size: 818 6395 DraIII CAC_nnn'GTG Cuts at: 2944 2944 Size: 7213 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6220 809 Size: 507 4904 1802 Fragments arranged by size: 4904 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2201 3660 4660 Size: 54 1425 50 275 18 371 1459 1000 Cuts at: 4660 5549 7178 8 Size: 889 1629 43 Fragments arranged by size: 1629 1459 1425 1000 889 371 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4660 1537 Size: 275 2848 4090 Fragments arranged by size: 4090 2848 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2312 2447 2984 3509 4106 5143 1353 Size: 819 140 135 537 525 597 1037 3423 Fragments arranged by size: 3423 1037 819 597 537 525 140 135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5800 6174 6320 387 Size: 1064 138 4211 374 146 1280 Fragments arranged by size: 4211 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3813 3813 Size: 7213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2278 2947 3533 3555 3599 4373 5276 Size: 393 646 669 586 22 44 774 903 Cuts at: 5276 5873 6660 6928 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 903 787 774 669 646 597 586 393 268 44 22 EcoO109I rG'GnC_Cy Cuts at: 2110 3793 4417 2110 Size: 1683 624 4906 Fragments arranged by size: 4906 1683 624 EcoRI G'AATT_C Cuts at: 4522 4900 4522 Size: 378 6835 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3124 4120 3124 Size: 996 6217 FspI TGC'GCA Cuts at: 2539 5719 2539 Size: 3180 4033 HaeII r_GCGC'y Cuts at: 1678 2031 3549 6360 1678 Size: 353 1518 2811 2531 Fragments arranged by size: 2811 2531 1518 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2251 2283 2412 2444 2952 Size: 32 135 32 679 32 129 32 508 Cuts at: 2952 2984 3086 3118 5854 5886 5928 5960 1373 Size: 32 102 32 2736 32 42 32 2626 Fragments arranged by size: 2736 2626 679 508 135 129 102 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4557 4803 669 Size: 988 281 2619 246 3079 Fragments arranged by size: 3079 2619 988 281 246 HindIII A'AGCT_T Cuts at: 748 2314 2758 748 Size: 1566 444 5203 Fragments arranged by size: 5203 1566 444 HpaI GTT'AAC Cuts at: 4803 4803 Size: 7213 KpnI G_GTAC'C Cuts at: 4538 4538 Size: 7213 MfeI C'AATT_G Cuts at: 4812 4812 Size: 7213 MluI A'CGCG_T Cuts at: 7089 7089 Size: 7213 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2790 6327 6511 1305 Size: 1485 3537 184 2007 Fragments arranged by size: 3537 2007 1485 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3662 10 Size: 54 1425 343 1830 3561 Fragments arranged by size: 3561 1830 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4377 5171 5530 5689 518 Size: 648 3211 794 359 159 2042 Fragments arranged by size: 3211 2042 794 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2783 4196 4217 4295 4629 5306 6454 6699 Size: 1177 1413 21 78 334 677 1148 245 Cuts at: 6699 1606 Size: 2120 Fragments arranged by size: 2120 1413 1177 1148 677 334 245 78 21 NcoI C'CATG_G Cuts at: 513 1065 2129 513 Size: 552 1064 5597 Fragments arranged by size: 5597 1064 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7213 NheI G'CTAG_C Cuts at: 1052 4063 4277 1052 Size: 3011 214 3988 Fragments arranged by size: 3988 3011 214 NotI GC'GGCC_GC Cuts at: 4660 4660 Size: 7213 NsiI A_TGCA'T Cuts at: 4152 4152 Size: 7213 NspI r_CATG'y Cuts at: 1132 1229 2291 2554 3592 4150 4267 4571 6116 Size: 97 1062 263 1038 558 117 304 1545 Cuts at: 6116 1132 Size: 2229 Fragments arranged by size: 2229 1545 1062 1038 558 304 263 117 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7213 PciI A'CATG_T Cuts at: 6112 6112 Size: 7213 PflMI CCAn_nnn'nTGG Cuts at: 1108 2508 1108 Size: 1400 5813 PfoI T'CCnGG_A Cuts at: 3615 3615 Size: 7213 PmeI GTTT'AAAC Cuts at: 4517 4517 Size: 7213 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2562 2594 5282 5314 6795 6827 2562 Size: 32 2688 32 1481 32 2948 Fragments arranged by size: 2948 2688 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2110 3793 2110 Size: 1683 5530 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7213 PsiI TTA'TAA Cuts at: 3597 3654 4783 3597 Size: 57 1129 6027 Fragments arranged by size: 6027 1129 57 PspOMI G'GGCC_C Cuts at: 4416 4416 Size: 7213 PstI C_TGCA'G Cuts at: 830 4425 4565 830 Size: 3595 140 3478 Fragments arranged by size: 3595 3478 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7213 PvuII CAG'CTG Cuts at: 4196 4217 4295 4196 Size: 21 78 7114 Fragments arranged by size: 7114 78 21 SacI G_AGCT'C Cuts at: 3815 3815 Size: 7213 SalI G'TCGA_C Cuts at: 1936 4555 1936 Size: 2619 4594 SapI GCTCTTCn'nnn_ Cuts at: 2984 4106 2984 Size: 1122 6091 SbfI CC_TGCA'GG Cuts at: 4565 4565 Size: 7213 ScaI AGT'ACT Cuts at: 5461 7173 5461 Size: 1712 5501 SfcI C'TryA_G Cuts at: 826 1045 3378 3550 4421 4561 5696 6377 6568 Size: 219 2333 172 871 140 1135 681 191 Cuts at: 6568 826 Size: 1471 Fragments arranged by size: 2333 1471 1135 871 681 219 191 172 140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7213 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7213 SmaI CCC'GGG Cuts at: 1872 4540 1872 Size: 2668 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3356 3407 4427 5317 6218 6480 Size: 197 926 1413 51 1020 890 901 262 Cuts at: 6480 820 Size: 1553 Fragments arranged by size: 1553 1413 1020 926 901 890 262 197 51 SnaBI TAC'GTA Cuts at: 493 5004 493 Size: 4511 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7213 SphI G_CATG'C Cuts at: 1132 2291 4571 1132 Size: 1159 2280 3774 Fragments arranged by size: 3774 2280 1159 SspI AAT'ATT Cuts at: 5 52 5137 5 Size: 47 5085 2081 Fragments arranged by size: 5085 2081 47 StuI AGG'CCT Cuts at: 1517 3676 4330 1517 Size: 2159 654 4400 Fragments arranged by size: 4400 2159 654 StyI C'CwwG_G Cuts at: 513 1065 2129 2332 3499 3724 3838 3914 4652 Size: 552 1064 203 1167 225 114 76 738 Cuts at: 4652 513 Size: 3074 Fragments arranged by size: 3074 1167 1064 738 552 225 203 114 76 SwaI ATTT'AAAT Cuts at: 3699 3699 Size: 7213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2711 3305 3941 5240 5257 5410 5595 Size: 1255 1131 594 636 1299 17 153 185 Cuts at: 5595 6040 325 Size: 445 1498 Fragments arranged by size: 1498 1299 1255 1131 636 594 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3564 3831 3939 Size: 274 80 33 51 1443 1587 267 108 Cuts at: 3939 5459 7171 96 Size: 1520 1712 138 Fragments arranged by size: 1712 1587 1520 1443 274 267 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7173 7205 7173 Size: 32 7181 Tth111I GACn'n_nGTC Cuts at: 1316 3100 4439 1316 Size: 1784 1339 4090 Fragments arranged by size: 4090 1784 1339 XbaI T'CTAG_A Cuts at: 4549 4549 Size: 7213 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2127 3486 2127 Size: 1359 5854 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7213 XmaI C'CCGG_G Cuts at: 1870 4538 1870 Size: 2668 4545 XmnI GAAnn'nnTTC Cuts at: 3900 5342 3900 Size: 1442 5771 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5021 276 Size: 53 83 186 936 3487 2468 Fragments arranged by size: 3487 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbeI BsiWI Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI