Restriction map for
pFN21AB8619
(Circular) (Six-base) MAPSORT of: pFN21AB8619.seq Check: 668 from: 1 to: 5653
pFN21AB8619 5653 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2227 3716 5100 5368 1632
Size: 595 1489 1384 268 1917
Fragments arranged by size:
1917 1489 1384 595 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2287 2857 2941 3396 4968 2287
Size: 570 84 455 1572 2972
Fragments arranged by size:
2972 1572 570 455 84
ApaI G_GGCC'C
Cuts at: 2592 2592
Size: 5653
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2036 2962 3188 3340 5449 1493
Size: 543 926 226 152 2109 1697
Fragments arranged by size:
2109 1697 926 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2340 2530 2974 4429 618
Size: 325 217 627 553 190 444 1455 1842
Fragments arranged by size:
1842 1455 627 553 444 325 217 190
BanII G_rGCy'C
Cuts at: 1391 2141 2592 2972 1391
Size: 750 451 380 4072
Fragments arranged by size:
4072 750 451 380
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2632 928
Size: 971 733 3949
Fragments arranged by size:
3949 971 733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2396 2822 4264
Size: 107 122 71 631 605 724 426 1442
Cuts at: 4264 136
Size: 1525
Fragments arranged by size:
1525 1442 724 631 605 426 122 107
71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2535 2592 3714 4870 1790
Size: 331 414 57 1122 1156 2573
Fragments arranged by size:
2573 1156 1122 414 331 57
BmgBI CAC'GTC
Cuts at: 1318 2269 1318
Size: 951 4702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2470 3384 4342
Size: 416 53 83 399 24 1053 914 958
Cuts at: 4342 442
Size: 1753
Fragments arranged by size:
1753 1053 958 914 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 4313 1239
Size: 1385 1689 2579
Fragments arranged by size:
2579 1689 1385
Bpu10I CC'TnA_GC
Cuts at: 2140 2140
Size: 5653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 3444 493
Size: 711 918 1322 2702
Fragments arranged by size:
2702 1322 918 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2292 2863 4086 4484 4758 4905 5316 2292
Size: 571 1223 398 274 147 411 2629
Fragments arranged by size:
2629 1223 571 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2597 2612 2627 2642 1178
Size: 30 165 30 1194 15 15 15 4189
Fragments arranged by size:
4189 1194 165 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2189 2192 2461 2868 2871 1186
Size: 871 132 3 269 407 3 3968
Fragments arranged by size:
3968 871 407 269 132 3 3
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2360 3103 3864 4892 1540
Size: 275 186 15 344 743 761 1028 2301
Fragments arranged by size:
2301 1028 761 743 344 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 3714 3799 4870 3714
Size: 85 1071 4497
Fragments arranged by size:
4497 1071 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2141 2535 2592 2972 3714 3799
Size: 399 331 20 394 57 380 742 85
Cuts at: 3799 4870 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 380 331 85
57 20
BspEI T'CCGG_A
Cuts at: 2863 5316 2863
Size: 2453 3200
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2347 2514 794
Size: 1553 167 3933
Fragments arranged by size:
3933 1553 167
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 2488 1184
Size: 1304 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 2776 2776
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2362 2382 2983 3350 3735 3752 5204 5290
Size: 390 20 601 367 385 17 1452 86
Cuts at: 5290 5302 5648 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 601 390 385 367 346 86
20 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2133 2493 2649 2751 2796 2826
Size: 552 939 129 360 156 102 45 30
Cuts at: 2826 513
Size: 3340
Fragments arranged by size:
3340 939 552 360 156 129 102 45
30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2896 487
Size: 683 56 1670 3244
Fragments arranged by size:
3244 1670 683 56
BtsI GCAGTG_nn'
Cuts at: 2306 2693 3169 3963 3983 2306
Size: 387 476 794 20 3976
Fragments arranged by size:
3976 794 476 387 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DraIII CAC_nnn'GTG
Cuts at: 2488 2488
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2130 2323
Size: 54 1425 50 275 18 183 117 193
Cuts at: 2323 2357 2409 2778 2823 3100 3989 5618 8
Size: 34 52 369 45 277 889 1629 43
Fragments arranged by size:
1629 1425 889 369 277 275 193 183
117 54 52 50 45 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2357 3100 1537
Size: 275 201 344 743 4090
Fragments arranged by size:
4090 743 344 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 3583 1353
Size: 2230 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2227 2624 3716 4313 5100 5368 1239
Size: 393 595 397 1092 597 787 268 1524
Fragments arranged by size:
1524 1092 787 597 595 397 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2284 2452 2588 2589 2284
Size: 168 136 1 5348
Fragments arranged by size:
5348 168 136 1
EcoRI G'AATT_C
Cuts at: 2036 2962 3340 2036
Size: 926 378 4349
Fragments arranged by size:
4349 926 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 5653
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 2705 4800 1678
Size: 1027 2095 2531
Fragments arranged by size:
2531 2095 1027
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373
Size: 32 135 32 2722 32 42 32 2626
Fragments arranged by size:
2722 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2325 2780 2825 10
Size: 54 1425 343 493 455 45 2838
Fragments arranged by size:
2838 1425 493 455 343 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 2672 3611 3970 4129 518
Size: 648 1482 24 939 359 159 2042
Fragments arranged by size:
2042 1482 939 648 359 159 24
MspA1I CmG'CkG
Cuts at: 1606 2104 3069 3746 4894 5139 1606
Size: 498 965 677 1148 245 2120
Fragments arranged by size:
2120 1148 965 677 498 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2649 2826 513
Size: 552 939 645 177 3340
Fragments arranged by size:
3340 939 645 552 177
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2710 1348
Size: 1362 4291
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PfoI T'CCnGG_A
Cuts at: 2286 2574 2601 2286
Size: 288 27 5338
Fragments arranged by size:
5338 288 27
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PmlI CAC'GTG
Cuts at: 2122 2122
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 2452 2284
Size: 168 5485
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PspOMI G'GGCC_C
Cuts at: 2588 2588
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2205 2688 3005 830
Size: 1375 483 317 3478
Fragments arranged by size:
3478 1375 483 317
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2104 2104
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SanDI GG'GwC_CC
Cuts at: 2452 2452
Size: 5653
SbfI CC_TGCA'GG
Cuts at: 2205 3005 2205
Size: 800 4853
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2201 2467 2684 3001 4136 4817 5008
Size: 219 1156 266 217 317 1135 681 191
Cuts at: 5008 826
Size: 1471
Fragments arranged by size:
1471 1156 1135 681 317 266 219 217
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 4658 4920 820
Size: 197 926 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2004 2649 2678 2826 3092 513
Size: 552 939 645 29 148 266 3074
Fragments arranged by size:
3074 939 645 552 266 148 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3697 3850 4035 4480 325
Size: 1255 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 3899 5611
Size: 274 80 33 51 1443 864 1058 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1712 1443 1058 864 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2597 2629 5613 5645 2597
Size: 32 2984 32 2605
Fragments arranged by size:
2984 2605 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2056 2056
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI