Restriction map for
pFN21AB8616
(Circular) (Six-base) MAPSORT of: pFN21AB8616.seq Check: 5427 from: 1 to: 5809
pFN21AB8616 5809 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2407 3619 278
Size: 53 83 186 936 871 1212 2468
Fragments arranged by size:
2468 1212 936 871 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3152 1937
Size: 1215 4594
Acc65I G'GTAC_C
Cuts at: 2488 3130 2488
Size: 642 5167
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 2523 2924 3036 3872 5256 5524 1632
Size: 786 105 401 112 836 1384 268 1917
Fragments arranged by size:
1917 1384 836 786 401 268 112 105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 3091 4538 3091
Size: 1447 4362
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5809
AlwNI CAG_nnn'CTG
Cuts at: 3552 5124 3552
Size: 1572 4237
ApaI G_GGCC'C
Cuts at: 2465 2465
Size: 5809
ApaLI G'TGCA_C
Cuts at: 3866 5022 3866
Size: 1156 4653
ApoI r'AATT_y
Cuts at: 1493 2120 2305 2747 3104 3118 3344 3496 5605
Size: 627 185 442 357 14 226 152 2109
Cuts at: 5605 1493
Size: 1697
Fragments arranged by size:
2109 1697 627 442 357 226 185 152
14
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 3134 1870
Size: 73 99 1092 4545
Fragments arranged by size:
4545 1092 99 73
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2488 3130 4585 618
Size: 325 217 627 701 642 1455 1842
Fragments arranged by size:
1842 1455 701 642 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2465 3128 1391
Size: 643 431 663 4072
Fragments arranged by size:
4072 663 643 431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3042 928
Size: 971 1143 3695
Fragments arranged by size:
3695 1143 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4000 4034 1903
Size: 34 2063 34 3678
Fragments arranged by size:
3678 2063 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3701 4911 3701
Size: 1210 4599
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4420 136
Size: 107 122 71 631 605 2748 1525
Fragments arranged by size:
2748 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2154 5804 2154
Size: 3650 2159
BlpI GC'TnA_GC
Cuts at: 3226 3226
Size: 5809
Bme1580I G_kGCm'C
Cuts at: 1790 2465 2736 3870 5026 1790
Size: 675 271 1134 1156 2573
Fragments arranged by size:
2573 1156 1134 675 271
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2723 3540 4498
Size: 416 53 83 399 24 1306 817 958
Cuts at: 4498 442
Size: 1753
Fragments arranged by size:
1753 1306 958 817 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2829 2829
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4469 1239
Size: 3230 2579
Bpu10I CC'TnA_GC
Cuts at: 2255 2255
Size: 5809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3934 4799 5097 3934
Size: 865 298 4646
Fragments arranged by size:
4646 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2817 4472 882
Size: 617 1318 1655 2219
Fragments arranged by size:
2219 1655 1318 617
BsaAI yAC'GTr
Cuts at: 493 1204 3600 493
Size: 711 2396 2702
Fragments arranged by size:
2702 2396 711
BsaBI GATnn'nnATC
Cuts at: 5803 5803
Size: 5809
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2404 3616 3998 4633
Size: 53 83 186 936 871 1212 382 635
Cuts at: 4633 275
Size: 1451
Fragments arranged by size:
1451 1212 936 871 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4242 4640 4914 5061 5472 4242
Size: 398 274 147 411 4579
Fragments arranged by size:
4579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5584
Fragments arranged by size:
5584 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2981 1186
Size: 1795 4014
BseYI C'CCAG_C
Cuts at: 2464 2736 5012 5508 2464
Size: 272 2276 496 2765
Fragments arranged by size:
2765 2276 496 272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5236
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3259 4020 5048 1540
Size: 275 186 1258 761 1028 2301
Fragments arranged by size:
2301 1258 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 3870 3955 5026 2012
Size: 1858 85 1071 2795
Fragments arranged by size:
2795 1858 1071 85
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2034 2465 2736 3128 3870 3955
Size: 399 222 22 431 271 392 742 85
Cuts at: 3955 5026 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 431 399 392 271 222
85 22
BspEI T'CCGG_A
Cuts at: 5472 5472
Size: 5809
BspHI T'CATG_A
Cuts at: 3698 5428 3698
Size: 1730 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3164 844
Size: 2320 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2833 3696 1394
Size: 429 1010 863 3507
Fragments arranged by size:
3507 1010 863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2187 2604 2794 4304 4478 65
Size: 1183 939 417 190 1510 174 1396
Fragments arranged by size:
1510 1396 1183 939 417 190 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4453 4640 1700
Size: 110 2643 187 2869
Fragments arranged by size:
2869 2643 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 3869 4881 3869
Size: 1012 4797
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5809
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstXI CCAn_nnnn'nTGG
Cuts at: 2399 2807 2399
Size: 408 5401
BstYI r'GATC_y
Cuts at: 1972 2154 3139 3506 3891 3908 5360 5446 5458
Size: 182 985 367 385 17 1452 86 12
Cuts at: 5458 5804 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 985 385 367 346 182 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2020 2522 513
Size: 552 955 502 3800
Fragments arranged by size:
3800 955 552 502
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 3325 4119 4139 3325
Size: 794 20 4995
Fragments arranged by size:
4995 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 2752 3113 2752
Size: 361 5448
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2403 4816 809
Size: 507 1087 2413 1802
Fragments arranged by size:
2413 1802 1087 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3256 4145 5774
Size: 54 1425 50 275 18 1426 889 1629
Cuts at: 5774 8
Size: 43
Fragments arranged by size:
1629 1426 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3256 1537
Size: 275 1444 4090
Fragments arranged by size:
4090 1444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 2798 2823 3739 1353
Size: 1068 377 25 916 3423
Fragments arranged by size:
3423 1068 916 377 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4396 4770 4916 387
Size: 1064 138 2807 374 146 1280
Fragments arranged by size:
2807 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2418 2523 2924 3036 3872 4469 5256
Size: 393 786 105 401 112 836 597 787
Cuts at: 5256 5524 1239
Size: 268 1524
Fragments arranged by size:
1524 836 787 786 597 401 393 268
112 105
EcoO109I rG'GnC_Cy
Cuts at: 2461 2461
Size: 5809
EcoRI G'AATT_C
Cuts at: 2305 3104 3118 3496 2305
Size: 799 14 378 4618
Fragments arranged by size:
4618 799 378 14
FspI TGC'GCA
Cuts at: 2111 4315 2111
Size: 2204 3605
HaeII r_GCGC'y
Cuts at: 1678 2095 4956 1678
Size: 417 2861 2531
Fragments arranged by size:
2861 2531 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 3019 3051 3077
Size: 32 135 32 743 32 672 32 26
Cuts at: 3077 3080 3109 3112 4450 4482 4524 4556 1373
Size: 3 29 3 1338 32 42 32 2626
Fragments arranged by size:
2626 1338 743 672 135 42 32 32
32 32 32 32 29 26 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3153 3399 669
Size: 988 281 1215 246 3079
Fragments arranged by size:
3079 1215 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5809
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KpnI G_GTAC'C
Cuts at: 2492 3134 2492
Size: 642 5167
MfeI C'AATT_G
Cuts at: 2268 3408 2268
Size: 1140 4669
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4923 5107 1305
Size: 3618 184 2007
Fragments arranged by size:
3618 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3987
Fragments arranged by size:
3987 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2702 2859 3767 4126 4285 518
Size: 648 1536 157 908 359 159 2042
Fragments arranged by size:
2042 1536 908 648 359 159 157
MspA1I CmG'CkG
Cuts at: 1606 2022 2059 2524 2740 2911 3225 3902 5050
Size: 416 37 465 216 171 314 677 1148
Cuts at: 5050 5295 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 465 416 314 245 216
171 37
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5257
NdeI CA'TA_TG
Cuts at: 387 2898 387
Size: 2511 3298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 3167 4712 1132
Size: 97 1938 1545 2229
Fragments arranged by size:
2229 1938 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5809
PfoI T'CCnGG_A
Cuts at: 2341 2341
Size: 5809
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3033 3379 3033
Size: 346 5463
PspOMI G'GGCC_C
Cuts at: 2461 2461
Size: 5809
PstI C_TGCA'G
Cuts at: 830 3161 830
Size: 2331 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
PvuII CAG'CTG
Cuts at: 2740 2911 2740
Size: 171 5638
SacII CC_GC'GG
Cuts at: 2023 2525 2023
Size: 502 5307
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SapI GCTCTTCn'nnn_
Cuts at: 2823 2823
Size: 5809
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 2647 4057 5769 2647
Size: 1410 1712 2687
Fragments arranged by size:
2687 1712 1410
SfcI C'TryA_G
Cuts at: 826 1045 2355 3157 4292 4973 5164 826
Size: 219 1310 802 1135 681 191 1471
Fragments arranged by size:
1471 1310 1135 802 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 3136 1872
Size: 1264 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3913 4814 5076 820
Size: 197 926 1970 901 262 1553
Fragments arranged by size:
1970 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 2335 152
Size: 2183 3626
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SspI AAT'ATT
Cuts at: 5 52 3016 3733 5
Size: 47 2964 717 2081
Fragments arranged by size:
2964 2081 717 47
StuI AGG'CCT
Cuts at: 1517 2664 1517
Size: 1147 4662
StyI C'CwwG_G
Cuts at: 513 1065 2614 2931 3248 513
Size: 552 1549 317 317 3074
Fragments arranged by size:
3074 1549 552 317 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3836 3853 4006 4191 4636 325
Size: 1255 2256 17 153 185 445 1498
Fragments arranged by size:
2256 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 2645 4055
Size: 274 80 33 51 1443 468 200 1410
Cuts at: 4055 5767 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1410 468 274 200 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5769 5801 5769
Size: 32 5777
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 3134 1870
Size: 1264 4545
XmnI GAAnn'nnTTC
Cuts at: 3938 3938
Size: 5809
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2405 3617 276
Size: 53 83 186 936 871 1212 2468
Fragments arranged by size:
2468 1212 936 871 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI