Restriction map for
pFN21AB8604
(Circular) (Six-base) MAPSORT of: pFN21AB8604.seq Check: 4834 from: 1 to: 5944
pFN21AB8604 5944 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2393 2460 3754 278
Size: 53 83 186 936 857 67 1294 2468
Fragments arranged by size:
2468 1294 936 857 186 83 67 53
AccI GT'mk_AC
Cuts at: 1937 3287 1937
Size: 1350 4594
Acc65I G'GTAC_C
Cuts at: 3265 3265
Size: 5944
AclI AA'CG_TT
Cuts at: 1563 3243 4071 4444 1563
Size: 1680 828 373 3063
Fragments arranged by size:
3063 1680 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 2876 3026 4007 5391 5659 1632
Size: 995 249 150 981 1384 268 1917
Fragments arranged by size:
1917 1384 995 981 268 249 150
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5747
AflIII A'CryG_T
Cuts at: 1203 4843 5820 1203
Size: 3640 977 1327
Fragments arranged by size:
3640 1327 977
AgeI A'CCGG_T
Cuts at: 4775 4775
Size: 5944
AhdI GACnn_n'nnGTC
Cuts at: 4673 4673
Size: 5944
AleI CACnn'nnGTG
Cuts at: 1166 2411 1166
Size: 1245 4699
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2429 2915 1184
Size: 1245 486 4213
Fragments arranged by size:
4213 1245 486
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2420 2452 2771 2803 2420
Size: 32 319 32 5561
Fragments arranged by size:
5561 319 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2470 2809 3687 5259 2470
Size: 339 878 1572 3155
Fragments arranged by size:
3155 1572 878 339
ApaI G_GGCC'C
Cuts at: 2218 2218
Size: 5944
ApaLI G'TGCA_C
Cuts at: 4001 5157 4001
Size: 1156 4788
ApoI r'AATT_y
Cuts at: 1493 3253 3479 3631 5740 1493
Size: 1760 226 152 2109 1697
Fragments arranged by size:
2109 1760 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4498 160
Size: 872 3466 1606
Fragments arranged by size:
3466 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2223 2249 3269 1870
Size: 73 123 157 26 1020 4545
Fragments arranged by size:
4545 1020 157 123 73 26
BamHI G'GATC_C
Cuts at: 3274 3641 3274
Size: 367 5577
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3152 3265 4720 618
Size: 325 217 627 1365 113 1455 1842
Fragments arranged by size:
1842 1455 1365 627 325 217 113
BanII G_rGCy'C
Cuts at: 1391 2034 2218 2250 2256 2564 3045 3263 1391
Size: 643 184 32 6 308 481 218 4072
Fragments arranged by size:
4072 643 481 308 218 184 32 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2324 2615 928
Size: 971 425 291 4257
Fragments arranged by size:
4257 971 425 291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4135 4169 1903
Size: 34 2198 34 3678
Fragments arranged by size:
3678 2198 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3836 5046 3836
Size: 1210 4734
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5944
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2550 2753 4555
Size: 107 122 71 631 605 878 203 1802
Cuts at: 4555 136
Size: 1525
Fragments arranged by size:
1802 1525 878 631 605 203 122 107
71
BglII A'GATC_T
Cuts at: 5939 5939
Size: 5944
BlpI GC'TnA_GC
Cuts at: 2202 3361 2202
Size: 1159 4785
Bme1580I G_kGCm'C
Cuts at: 1790 2218 3143 4005 5161 1790
Size: 428 925 862 1156 2573
Fragments arranged by size:
2573 1156 925 862 428
BmgBI CAC'GTC
Cuts at: 1318 2497 1318
Size: 1179 4765
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3675 4633 442
Size: 416 53 83 399 24 2258 958 1753
Fragments arranged by size:
2258 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2417 2615 2654 2749 2924 4604 1239
Size: 950 228 198 39 95 175 1680 2579
Fragments arranged by size:
2579 1680 950 228 198 175 95 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4069 4934 5232 4069
Size: 865 298 4781
Fragments arranged by size:
4781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3080 4607 882
Size: 617 1581 1527 2219
Fragments arranged by size:
2219 1581 1527 617
BsaAI yAC'GTr
Cuts at: 493 1204 3735 493
Size: 711 2531 2702
Fragments arranged by size:
2702 2531 711
BsaBI GATnn'nnATC
Cuts at: 5938 5938
Size: 5944
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2390 2457 3751 4133
Size: 53 83 186 936 857 67 1294 382
Cuts at: 4133 4768 275
Size: 635 1451
Fragments arranged by size:
1451 1294 936 857 635 382 186 83
67 53
BsaWI w'CCGG_w
Cuts at: 2673 3099 4377 4775 5049 5196 5607 2673
Size: 426 1278 398 274 147 411 3010
Fragments arranged by size:
3010 1278 426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2450 1178
Size: 30 165 30 1017 30 4672
Fragments arranged by size:
4672 1017 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2135 2138 2147 2210 2435 2807 2810
Size: 940 9 3 9 63 225 372 3
Cuts at: 2810 1186
Size: 4320
Fragments arranged by size:
4320 940 372 225 63 9 9 3
3
BseYI C'CCAG_C
Cuts at: 2260 2958 3109 3119 5147 5643 2260
Size: 698 151 10 2028 496 2561
Fragments arranged by size:
2561 2028 698 496 151 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 1163
Size: 573 699 4672
Fragments arranged by size:
4672 699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3394 4155 5183 1540
Size: 275 186 1393 761 1028 2301
Fragments arranged by size:
2301 1393 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2910 3045 3083 4005 4090 5161 2910
Size: 135 38 922 85 1071 3693
Fragments arranged by size:
3693 1071 922 135 85 38
BsmI GAATG_Cn'
Cuts at: 3455 3548 3455
Size: 93 5851
BsmBI CGTCTCn'nnnn_
Cuts at: 2498 2498
Size: 5944
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2218 2250 2256 2564 2910 3045
Size: 399 244 184 32 6 308 346 135
Cuts at: 3045 3083 3143 3263 4005 4090 5161 1391
Size: 38 60 120 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 346 308 244 184
135 120 85 60 38 32 6
BspEI T'CCGG_A
Cuts at: 5607 5607
Size: 5944
BspHI T'CATG_A
Cuts at: 3833 5563 3833
Size: 1730 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3299 844
Size: 2455 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2065 3831 1394
Size: 429 242 1766 3507
Fragments arranged by size:
3507 1766 429 242
BsrDI GCAATG_nn'
Cuts at: 65 1248 4439 4613 65
Size: 1183 3191 174 1396
Fragments arranged by size:
3191 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3123 4588 4775 1700
Size: 110 1313 1465 187 2869
Fragments arranged by size:
2869 1465 1313 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5944
BssSI C'ACGA_G
Cuts at: 4004 5016 4004
Size: 1012 4932
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2015 794
Size: 1221 4723
BstBI TT'CG_AA
Cuts at: 4748 4748
Size: 5944
BstEII G'GTnAC_C
Cuts at: 1184 2371 2599 1184
Size: 1187 228 4529
Fragments arranged by size:
4529 1187 228
BstXI CCAn_nnnn'nTGG
Cuts at: 2280 3174 2280
Size: 894 5050
BstYI r'GATC_y
Cuts at: 1972 2473 3274 3641 4026 4043 5495 5581 5593
Size: 501 801 367 385 17 1452 86 12
Cuts at: 5593 5939 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 801 501 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2993 2993
Size: 5944
BtgI C'CryG_G
Cuts at: 513 1065 2004 2241 2511 3167 513
Size: 552 939 237 270 656 3290
Fragments arranged by size:
3290 939 656 552 270 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3214 487
Size: 683 56 1988 3217
Fragments arranged by size:
3217 1988 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 2517 3460 4254 4274 2042
Size: 475 943 794 20 3712
Fragments arranged by size:
3712 943 794 475 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5909
DraI TTT'AAA
Cuts at: 3248 3248
Size: 5944
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2456 4951 809
Size: 507 1140 2495 1802
Fragments arranged by size:
2495 1802 1140 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2508 2667 2724
Size: 54 1425 50 275 18 678 159 57
Cuts at: 2724 3391 4280 5909 8
Size: 667 889 1629 43
Fragments arranged by size:
1629 1425 889 678 667 275 159 57
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3391 1537
Size: 275 1579 4090
Fragments arranged by size:
4090 1579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2124 2133 2271 2939 3874 1353
Size: 759 12 9 138 668 935 3423
Fragments arranged by size:
3423 935 759 668 138 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2207 2726 4531 4905 5051 387
Size: 1064 138 618 519 1805 374 146 1280
Fragments arranged by size:
1805 1280 1064 618 519 374 146 138
EcoICRI GAG'CTC
Cuts at: 3043 3043
Size: 5944
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2417 2615 2627 2654 2749 2876
Size: 393 557 228 198 12 27 95 127
Cuts at: 2876 2924 3026 4007 4604 5391 5659 1239
Size: 48 102 981 597 787 268 1524
Fragments arranged by size:
1524 981 787 597 557 393 268 228
198 127 102 95 48 27 12
EcoO109I rG'GnC_Cy
Cuts at: 2056 2214 2215 2467 2056
Size: 158 1 252 5533
Fragments arranged by size:
5533 252 158 1
EcoRI G'AATT_C
Cuts at: 3253 3631 3253
Size: 378 5566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 5944
FspI TGC'GCA
Cuts at: 4450 4450
Size: 5944
HaeII r_GCGC'y
Cuts at: 1678 2772 5091 1678
Size: 1094 2319 2531
Fragments arranged by size:
2531 2319 1094
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4585 4617 4659 4691 1373
Size: 32 135 32 3013 32 42 32 2626
Fragments arranged by size:
3013 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3288 3534 669
Size: 988 281 1350 246 3079
Fragments arranged by size:
3079 1350 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5944
HpaI GTT'AAC
Cuts at: 3534 3534
Size: 5944
KpnI G_GTAC'C
Cuts at: 3269 3269
Size: 5944
MfeI C'AATT_G
Cuts at: 3543 3543
Size: 5944
MluI A'CGCG_T
Cuts at: 5820 5820
Size: 5944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3045 5058 5242 1305
Size: 1740 2013 184 2007
Fragments arranged by size:
2013 2007 1740 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 10
Size: 54 1425 343 837 3285
Fragments arranged by size:
3285 1425 837 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 2495 3172 3902 4261 4420 518
Size: 648 1245 84 677 730 359 159 2042
Fragments arranged by size:
2042 1245 730 677 648 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2167 2707 2854 3076 3360 4037 5185 5430
Size: 561 540 147 222 284 677 1148 245
Cuts at: 5430 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 561 540 284 245 222
147
NaeI GCC'GGC
Cuts at: 3125 3125
Size: 5944
NcoI C'CATG_G
Cuts at: 513 1065 2004 2241 2511 3167 513
Size: 552 939 237 270 656 3290
Fragments arranged by size:
3290 939 656 552 270 237
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5944
NgoMIV G'CCGG_C
Cuts at: 3123 3123
Size: 5944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5944
NotI GC'GGCC_GC
Cuts at: 3391 3391
Size: 5944
NspI r_CATG'y
Cuts at: 1132 1229 3068 3302 4847 1132
Size: 97 1839 234 1545 2229
Fragments arranged by size:
2229 1839 1545 234 97
PasI CC'CwG_GG
Cuts at: 1348 2330 1348
Size: 982 4962
PciI A'CATG_T
Cuts at: 4843 4843
Size: 5944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2470 3168 1108
Size: 1362 698 3884
Fragments arranged by size:
3884 1362 698
PmeI GTTT'AAAC
Cuts at: 3248 3248
Size: 5944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2420 2452 4013 4045 5526 5558 2420
Size: 32 1561 32 1481 32 2806
Fragments arranged by size:
2806 1561 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2056
Size: 5944
PshAI GACnn'nnGTC
Cuts at: 1613 2324 1613
Size: 711 5233
PsiI TTA'TAA
Cuts at: 3514 3514
Size: 5944
PspOMI G'GGCC_C
Cuts at: 2214 2214
Size: 5944
PspXI vC'TCGA_Gb
Cuts at: 2066 2066
Size: 5944
PstI C_TGCA'G
Cuts at: 830 2388 2814 2894 3296 830
Size: 1558 426 80 402 3478
Fragments arranged by size:
3478 1558 426 402 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5944
PvuII CAG'CTG
Cuts at: 2854 2854
Size: 5944
SacI G_AGCT'C
Cuts at: 3045 3045
Size: 5944
SalI G'TCGA_C
Cuts at: 1936 3286 1936
Size: 1350 4594
SanDI GG'GwC_CC
Cuts at: 2056 2056
Size: 5944
SbfI CC_TGCA'GG
Cuts at: 3296 3296
Size: 5944
ScaI AGT'ACT
Cuts at: 2534 4192 5904 2534
Size: 1658 1712 2574
Fragments arranged by size:
2574 1712 1658
SfcI C'TryA_G
Cuts at: 826 1045 2384 2810 2890 3292 4427 5108 5299
Size: 219 1339 426 80 402 1135 681 191
Cuts at: 5299 826
Size: 1471
Fragments arranged by size:
1471 1339 1135 681 426 402 219 191
80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5944
SmaI CCC'GGG
Cuts at: 1872 3271 1872
Size: 1399 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2066 4048 4949 5211 820
Size: 197 926 123 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 262 197 123
SnaBI TAC'GTA
Cuts at: 493 3735 493
Size: 3242 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5944
SphI G_CATG'C
Cuts at: 1132 3068 3302 1132
Size: 1936 234 3774
Fragments arranged by size:
3774 1936 234
SspI AAT'ATT
Cuts at: 5 52 3868 5
Size: 47 3816 2081
Fragments arranged by size:
3816 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5944
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2511 2548 3167 3201 3383
Size: 552 939 237 270 37 619 34 182
Cuts at: 3383 513
Size: 3074
Fragments arranged by size:
3074 939 619 552 270 237 182 37
34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2435 3971 3988 4141 4326 4771 325
Size: 1255 855 1536 17 153 185 445 1498
Fragments arranged by size:
1536 1498 1255 855 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 2970 4190
Size: 274 80 33 51 1443 555 438 1220
Cuts at: 4190 5902 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1220 555 438 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5904 5936 5904
Size: 32 5912
Tth111I GACn'n_nGTC
Cuts at: 1316 2717 1316
Size: 1401 4543
XbaI T'CTAG_A
Cuts at: 3280 3280
Size: 5944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2773 2773
Size: 5944
XhoI C'TCGA_G
Cuts at: 1943 2066 1943
Size: 123 5821
XmaI C'CCGG_G
Cuts at: 1870 3269 1870
Size: 1399 4545
XmnI GAAnn'nnTTC
Cuts at: 4073 4073
Size: 5944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2391 2458 3752 276
Size: 53 83 186 936 857 67 1294 2468
Fragments arranged by size:
2468 1294 936 857 186 83 67 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI