Restriction map for
pFN21AB8598
(Circular) (Six-base) MAPSORT of: pFN21AB8598.seq Check: 1803 from: 1 to: 7033
pFN21AB8598 7033 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2083 2083
Size: 7033
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4843 278
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3815 4376 1937
Size: 1878 561 4594
Fragments arranged by size:
4594 1878 561
Acc65I G'GTAC_C
Cuts at: 4354 4354
Size: 7033
AclI AA'CG_TT
Cuts at: 1563 5160 5533 1563
Size: 3597 373 3063
Fragments arranged by size:
3597 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2060 3500 3824 5096 6480 6748 1632
Size: 428 1440 324 1272 1384 268 1917
Fragments arranged by size:
1917 1440 1384 1272 428 324 268
AflII C'TTAA_G
Cuts at: 820 1017 2279 2315 820
Size: 197 1262 36 5538
Fragments arranged by size:
5538 1262 197 36
AflIII A'CryG_T
Cuts at: 1203 5932 6909 1203
Size: 4729 977 1327
Fragments arranged by size:
4729 1327 977
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 5762 5762
Size: 7033
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3635 1184
Size: 2451 4582
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2187 2219 2187
Size: 32 7001
AlwNI CAG_nnn'CTG
Cuts at: 2777 3877 4776 6348 2777
Size: 1100 899 1572 3462
Fragments arranged by size:
3462 1572 1100 899
ApaI G_GGCC'C
Cuts at: 2672 2672
Size: 7033
ApaLI G'TGCA_C
Cuts at: 5090 6246 5090
Size: 1156 5877
ApoI r'AATT_y
Cuts at: 1493 2285 2354 2409 2753 2907 2921 2929 4342
Size: 792 69 55 344 154 14 8 1413
Cuts at: 4342 4568 4720 6829 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1413 792 344 226 154 152
69 55 14 8
AseI AT'TA_AT
Cuts at: 160 1032 5587 160
Size: 872 4555 1606
Fragments arranged by size:
4555 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 4236 4358 1870
Size: 73 2293 122 4545
Fragments arranged by size:
4545 2293 122 73
AvrII C'CTAG_G
Cuts at: 3001 3001
Size: 7033
BamHI G'GATC_C
Cuts at: 4363 4730 4363
Size: 367 6666
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4354 5809 618
Size: 325 217 627 2567 1455 1842
Fragments arranged by size:
2567 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2139 2248 2672 3629 4352 1391
Size: 748 109 424 957 723 4072
Fragments arranged by size:
4072 957 748 723 424 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3339 3352 3381 4125 928
Size: 971 1440 13 29 744 3836
Fragments arranged by size:
3836 1440 971 744 29 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5224 5258 1903
Size: 34 3287 34 3678
Fragments arranged by size:
3678 3287 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4925 6135 4925
Size: 1210 5823
BclI T'GATC_A
Cuts at: 1548 4248 1548
Size: 2700 4333
BfrBI ATG'CAT
Cuts at: 2657 4260 2657
Size: 1603 5430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3729 5644 136
Size: 107 122 71 631 605 2057 1915 1525
Fragments arranged by size:
2057 1915 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2061 3550 7028 2061
Size: 1489 3478 2066
Fragments arranged by size:
3478 2066 1489
BlpI GC'TnA_GC
Cuts at: 3623 4450 3623
Size: 827 6206
Bme1580I G_kGCm'C
Cuts at: 1790 2672 5094 6250 1790
Size: 882 2422 1156 2573
Fragments arranged by size:
2573 2422 1156 882
BmgBI CAC'GTC
Cuts at: 1318 3744 1318
Size: 2426 4607
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2765 4764 5722
Size: 416 53 83 399 24 1348 1999 958
Cuts at: 5722 442
Size: 1753
Fragments arranged by size:
1999 1753 1348 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3523 3523
Size: 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 2259 4207 5693 1239
Size: 981 39 1948 1486 2579
Fragments arranged by size:
2579 1948 1486 981 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2831 5158 6023 6321 2831
Size: 2327 865 298 3543
Fragments arranged by size:
3543 2327 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5696 882
Size: 617 4197 2219
Fragments arranged by size:
4197 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2629 4824 493
Size: 711 1425 2195 2702
Fragments arranged by size:
2702 2195 1425 711
BsaBI GATnn'nnATC
Cuts at: 3830 7027 3830
Size: 3197 3836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3812 4840 5222 5857
Size: 53 83 186 936 2279 1028 382 635
Cuts at: 5857 275
Size: 1451
Fragments arranged by size:
2279 1451 1028 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5466 5864 6138 6285 6696 5466
Size: 398 274 147 411 5803
Fragments arranged by size:
5803 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2187 2217 2497 2527 3269
Size: 30 165 30 784 30 280 30 742
Cuts at: 3269 3299 3759 3789 1178
Size: 30 460 30 4422
Fragments arranged by size:
4422 784 742 460 280 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2208 2256 3332 1186
Size: 1022 48 1076 4887
Fragments arranged by size:
4887 1076 1022 48
BseYI C'CCAG_C
Cuts at: 6236 6732 6236
Size: 496 6537
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3890 1163
Size: 573 2154 4306
Fragments arranged by size:
4306 2154 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4483 5244 6272 1540
Size: 275 186 2482 761 1028 2301
Fragments arranged by size:
2482 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2248 3629 5094 5179 6250 2139
Size: 109 1381 1465 85 1071 2922
Fragments arranged by size:
2922 1465 1381 1071 109 85
BsmI GAATG_Cn'
Cuts at: 3155 3612 4544 4637 3155
Size: 457 932 93 5551
Fragments arranged by size:
5551 932 457 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2248 2672 3629 4352 5094 5179
Size: 399 349 109 424 957 723 742 85
Cuts at: 5179 6250 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 957 742 723 424 399 349
109 85
BspEI T'CCGG_A
Cuts at: 6696 6696
Size: 7033
BspHI T'CATG_A
Cuts at: 2842 4158 4922 6652 2842
Size: 1316 764 1730 3223
Fragments arranged by size:
3223 1730 1316 764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 4388 844
Size: 1239 2305 3489
Fragments arranged by size:
3489 2305 1239
BsrBI CCG'CTC
Cuts at: 1394 1823 4920 1394
Size: 429 3097 3507
Fragments arranged by size:
3507 3097 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5528 5702 65
Size: 1183 4280 174 1396
Fragments arranged by size:
4280 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 5677 5864 1700
Size: 110 271 3596 187 2869
Fragments arranged by size:
3596 2869 271 187 110
BsrGI T'GTAC_A
Cuts at: 96 2097 2617 3103 3656 96
Size: 2001 520 486 553 3473
Fragments arranged by size:
3473 2001 553 520 486
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 2190 5093 6105 2190
Size: 2903 1012 3118
Fragments arranged by size:
3118 2903 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7033
BstBI TT'CG_AA
Cuts at: 5837 5837
Size: 7033
BstEII G'GTnAC_C
Cuts at: 1184 3226 1184
Size: 2042 4991
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2131
Size: 7033
BstYI r'GATC_y
Cuts at: 1972 2061 3550 4363 4730 5115 5132 6584 6670
Size: 89 1489 813 367 385 17 1452 86
Cuts at: 6670 6682 7028 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1489 1452 813 385 367 346 89
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2032 513
Size: 552 967 5514
Fragments arranged by size:
5514 967 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6294
Fragments arranged by size:
6294 683 56
BtsI GCAGTG_nn'
Cuts at: 4549 5343 5363 4549
Size: 794 20 6219
Fragments arranged by size:
6219 794 20
ClaI AT'CG_AT
Cuts at: 1554 2383 1554
Size: 829 6204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6998
DraI TTT'AAA
Cuts at: 2556 3084 4337 2556
Size: 528 1253 5252
Fragments arranged by size:
5252 1253 528
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6040 809
Size: 507 4724 1802
Fragments arranged by size:
4724 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 3730 4143
Size: 54 1425 50 275 18 285 1615 413
Cuts at: 4143 4480 5369 6998 8
Size: 337 889 1629 43
Fragments arranged by size:
1629 1615 1425 889 413 337 285 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4480 1537
Size: 275 2668 4090
Fragments arranged by size:
4090 2668 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2818 4963 1353
Size: 1465 2145 3423
Fragments arranged by size:
3423 2145 1465
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5620 5994 6140 387
Size: 1064 138 4031 374 146 1280
Fragments arranged by size:
4031 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2137 2246 3627 2137
Size: 109 1381 5543
Fragments arranged by size:
5543 1381 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2220 2259 3500 3824 4207 5096
Size: 393 428 160 39 1241 324 383 889
Cuts at: 5096 5693 6480 6748 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1241 889 787 597 428 393 383
324 268 160 39
EcoO109I rG'GnC_Cy
Cuts at: 2668 2669 3633 4204 2668
Size: 1 964 571 5497
Fragments arranged by size:
5497 964 571 1
EcoRI G'AATT_C
Cuts at: 2753 4342 4720 2753
Size: 1589 378 5066
Fragments arranged by size:
5066 1589 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 7033
FspI TGC'GCA
Cuts at: 5539 5539
Size: 7033
HaeII r_GCGC'y
Cuts at: 1678 6180 1678
Size: 4502 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 2497 2529 3535
Size: 32 135 32 498 32 395 32 1006
Cuts at: 3535 3567 5674 5706 5748 5780 1373
Size: 32 2107 32 42 32 2626
Fragments arranged by size:
2626 2107 1006 498 395 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2013 2679 3816 4051 4377 4623
Size: 988 281 75 666 1137 235 326 246
Cuts at: 4623 669
Size: 3079
Fragments arranged by size:
3079 1137 988 666 326 281 246 235
75
HindIII A'AGCT_T
Cuts at: 748 3486 748
Size: 2738 4295
HpaI GTT'AAC
Cuts at: 2679 4051 4623 2679
Size: 1372 572 5089
Fragments arranged by size:
5089 1372 572
KpnI G_GTAC'C
Cuts at: 4358 4358
Size: 7033
MfeI C'AATT_G
Cuts at: 4632 4632
Size: 7033
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3003 3660 6147 6331 1305
Size: 1698 657 2487 184 2007
Fragments arranged by size:
2487 2007 1698 657 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 10
Size: 54 1425 343 285 4926
Fragments arranged by size:
4926 1425 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3539 3617 4058 4163 4991 5350 5509
Size: 648 2373 78 441 105 828 359 159
Cuts at: 5509 518
Size: 2042
Fragments arranged by size:
2373 2042 828 648 441 359 159 105
78
MspA1I CmG'CkG
Cuts at: 1606 3877 4216 4449 5126 6274 6519 1606
Size: 2271 339 233 677 1148 245 2120
Fragments arranged by size:
2271 2120 1148 677 339 245 233
NaeI GCC'GGC
Cuts at: 2083 2083
Size: 7033
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6481
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7033
NgoMIV G'CCGG_C
Cuts at: 2081 2081
Size: 7033
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7033
NotI GC'GGCC_GC
Cuts at: 4480 4480
Size: 7033
NsiI A_TGCA'T
Cuts at: 2659 4262 2659
Size: 1603 5430
NspI r_CATG'y
Cuts at: 1132 1229 2971 3526 4391 5936 1132
Size: 97 1742 555 865 1545 2229
Fragments arranged by size:
2229 1742 1545 865 555 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7033
PciI A'CATG_T
Cuts at: 5932 5932
Size: 7033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7033
PfoI T'CCnGG_A
Cuts at: 2991 2991
Size: 7033
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2187 2219 2871 2903 5102 5134 6615 6647 2187
Size: 32 652 32 2199 32 1481 32 2573
Fragments arranged by size:
2573 2199 1481 652 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3633 4204 3633
Size: 571 6462
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7033
PsiI TTA'TAA
Cuts at: 2154 4029 4603 2154
Size: 1875 574 4584
Fragments arranged by size:
4584 1875 574
PspOMI G'GGCC_C
Cuts at: 2668 2668
Size: 7033
PspXI vC'TCGA_Gb
Cuts at: 4236 4236
Size: 7033
PstI C_TGCA'G
Cuts at: 830 4385 830
Size: 3555 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
PvuII CAG'CTG
Cuts at: 3877 4216 3877
Size: 339 6694
SacI G_AGCT'C
Cuts at: 2139 2248 3629 2139
Size: 109 1381 5543
Fragments arranged by size:
5543 1381 109
SalI G'TCGA_C
Cuts at: 1936 3814 4375 1936
Size: 1878 561 4594
Fragments arranged by size:
4594 1878 561
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 2749 5281 6993 2749
Size: 2532 1712 2789
Fragments arranged by size:
2789 2532 1712
SfcI C'TryA_G
Cuts at: 826 1045 4318 4381 5516 6197 6388 826
Size: 219 3273 63 1135 681 191 1471
Fragments arranged by size:
3273 1471 1135 681 219 191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7033
SmaI CCC'GGG
Cuts at: 1872 4360 1872
Size: 2488 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 2315 2810 4236 5137 6038
Size: 197 926 336 36 495 1426 901 901
Cuts at: 6038 6300 820
Size: 262 1553
Fragments arranged by size:
1553 1426 926 901 901 495 336 262
197 36
SnaBI TAC'GTA
Cuts at: 493 4824 493
Size: 4331 2702
SpeI A'CTAG_T
Cuts at: 152 2165 152
Size: 2013 5020
SphI G_CATG'C
Cuts at: 1132 4391 1132
Size: 3259 3774
SspI AAT'ATT
Cuts at: 5 52 4286 4957 5
Size: 47 4234 671 2081
Fragments arranged by size:
4234 2081 671 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7033
StyI C'CwwG_G
Cuts at: 513 1065 3001 4472 513
Size: 552 1936 1471 3074
Fragments arranged by size:
3074 1936 1471 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3896 5060 5077 5230 5415 5860 325
Size: 1255 2316 1164 17 153 185 445 1498
Fragments arranged by size:
2316 1498 1255 1164 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2097 2162 2347
Size: 274 80 33 51 1443 120 65 185
Cuts at: 2347 2617 2747 3103 3366 3501 3656 3843 5279
Size: 270 130 356 263 135 155 187 1436
Cuts at: 5279 6991 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1436 356 274 270 263 187
185 155 138 135 130 120 80 65
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6993 7025 6993
Size: 32 7001
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7033
XbaI T'CTAG_A
Cuts at: 2984 4369 2984
Size: 1385 5648
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4193 4193
Size: 7033
XhoI C'TCGA_G
Cuts at: 1943 4236 1943
Size: 2293 4740
XmaI C'CCGG_G
Cuts at: 1870 4358 1870
Size: 2488 4545
XmnI GAAnn'nnTTC
Cuts at: 5162 5162
Size: 7033
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4841 276
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI
NarI NruI PacI PmlI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI