Restriction map for
pFN21AB8596
(Circular) (Six-base) MAPSORT of: pFN21AB8596.seq Check: 6139 from: 1 to: 7399
pFN21AB8596 7399 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5209 278
Size: 53 83 186 936 3673 2468
Fragments arranged by size:
3673 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4157 4742 1937
Size: 2220 585 4594
Fragments arranged by size:
4594 2220 585
Acc65I G'GTAC_C
Cuts at: 4720 4720
Size: 7399
AclI AA'CG_TT
Cuts at: 1563 5526 5899 1563
Size: 3963 373 3063
Fragments arranged by size:
3963 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3347 3432 3671 3732 3945 4088 5462 6846
Size: 1715 85 239 61 213 143 1374 1384
Cuts at: 6846 7114 1632
Size: 268 1917
Fragments arranged by size:
1917 1715 1384 1374 268 239 213 143
85 61
AflII C'TTAA_G
Cuts at: 820 1017 3041 3568 820
Size: 197 2024 527 4651
Fragments arranged by size:
4651 2024 527 197
AflIII A'CryG_T
Cuts at: 1203 4561 6298 7275 1203
Size: 3358 1737 977 1327
Fragments arranged by size:
3358 1737 1327 977
AgeI A'CCGG_T
Cuts at: 6230 6230
Size: 7399
AhdI GACnn_n'nnGTC
Cuts at: 6128 6128
Size: 7399
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7399
AlwNI CAG_nnn'CTG
Cuts at: 2789 5142 6714 2789
Size: 2353 1572 3474
Fragments arranged by size:
3474 2353 1572
ApaLI G'TGCA_C
Cuts at: 5456 6612 5456
Size: 1156 6243
ApoI r'AATT_y
Cuts at: 1493 2222 2521 2610 2703 4543 4664 4708 4934
Size: 729 299 89 93 1840 121 44 226
Cuts at: 4934 5086 7195 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1840 1697 729 299 226 152 121
93 89 44
AseI AT'TA_AT
Cuts at: 160 1032 2847 5953 160
Size: 872 1815 3106 1606
Fragments arranged by size:
3106 1815 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7399
AvaI C'yCGr_G
Cuts at: 1870 1943 4724 1870
Size: 73 2781 4545
Fragments arranged by size:
4545 2781 73
BamHI G'GATC_C
Cuts at: 4729 5096 4729
Size: 367 7032
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 4453 4720 6175 618
Size: 325 217 627 555 2111 267 1455 1842
Fragments arranged by size:
2111 1842 1455 627 555 325 267 217
BanII G_rGCy'C
Cuts at: 1391 4340 4718 1391
Size: 2949 378 4072
Fragments arranged by size:
4072 2949 378
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2028 2366 3420 3677 4134 4332 928
Size: 971 129 338 1054 257 457 198 3995
Fragments arranged by size:
3995 1054 971 457 338 257 198 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5590 5624 1903
Size: 34 3653 34 3678
Fragments arranged by size:
3678 3653 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5291 6501 5291
Size: 1210 6189
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7399
BfrBI ATG'CAT
Cuts at: 2433 2433
Size: 7399
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6010 136
Size: 107 122 71 631 605 4338 1525
Fragments arranged by size:
4338 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3129 3607 7394 3129
Size: 478 3787 3134
Fragments arranged by size:
3787 3134 478
BlpI GC'TnA_GC
Cuts at: 4816 4816
Size: 7399
Bme1580I G_kGCm'C
Cuts at: 1790 5460 6616 1790
Size: 3670 1156 2573
Fragments arranged by size:
3670 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7399
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5130 6088 442
Size: 416 53 83 399 24 3713 958 1753
Fragments arranged by size:
3713 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2415 2504 3506 3617 6059 1239
Size: 1176 89 1002 111 2442 2579
Fragments arranged by size:
2579 2442 1176 1002 111 89
Bpu10I CC'TnA_GC
Cuts at: 2814 2887 2814
Size: 73 7326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3584 5524 6389 6687 3584
Size: 1940 865 298 4296
Fragments arranged by size:
4296 1940 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6062 882
Size: 617 4563 2219
Fragments arranged by size:
4563 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5190 493
Size: 711 3986 2702
Fragments arranged by size:
3986 2702 711
BsaBI GATnn'nnATC
Cuts at: 7393 7393
Size: 7399
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5206 5588 6223 275
Size: 53 83 186 936 3673 382 635 1451
Fragments arranged by size:
3673 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2062 2807 5832 6230 6504 6651 7062 2062
Size: 745 3025 398 274 147 411 2399
Fragments arranged by size:
3025 2399 745 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7174
Fragments arranged by size:
7174 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3515 1186
Size: 2329 5070
BseYI C'CCAG_C
Cuts at: 2354 6602 7098 2354
Size: 4248 496 2655
Fragments arranged by size:
4248 2655 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4094 1163
Size: 573 2358 4468
Fragments arranged by size:
4468 2358 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4849 5610 6638 1540
Size: 275 186 2848 761 1028 2301
Fragments arranged by size:
2848 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4340 5460 5545 6616 4340
Size: 1120 85 1071 5123
Fragments arranged by size:
5123 1120 1071 85
BsmI GAATG_Cn'
Cuts at: 2674 3730 4429 4910 5003 2674
Size: 1056 699 481 93 5070
Fragments arranged by size:
5070 1056 699 481 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4340 4718 5460 5545 6616 1391
Size: 399 2550 378 742 85 1071 2174
Fragments arranged by size:
2550 2174 1071 742 399 378 85
BspEI T'CCGG_A
Cuts at: 2062 7062 2062
Size: 5000 2399
BspHI T'CATG_A
Cuts at: 5288 7018 5288
Size: 1730 5669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4754 844
Size: 3910 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5286 1394
Size: 429 3463 3507
Fragments arranged by size:
3507 3463 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3094 3465 4476 5894 6068 65
Size: 1183 1846 371 1011 1418 174 1396
Fragments arranged by size:
1846 1418 1396 1183 1011 371 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3773 6043 6230 1700
Size: 110 1963 2270 187 2869
Fragments arranged by size:
2869 2270 1963 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7399
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7399
BssSI C'ACGA_G
Cuts at: 5459 6471 5459
Size: 1012 6387
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4435 794
Size: 3641 3758
BstBI TT'CG_AA
Cuts at: 6203 6203
Size: 7399
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7399
BstYI r'GATC_y
Cuts at: 1972 2215 2786 3129 3607 3673 4729 5096 5481
Size: 243 571 343 478 66 1056 367 385
Cuts at: 5481 5498 6950 7036 7048 7394 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1056 571 478 385 367 346
343 243 86 66 17 12
Bsu36I CC'TnA_GG
Cuts at: 3246 3246
Size: 7399
BtgI C'CryG_G
Cuts at: 513 1065 2167 513
Size: 552 1102 5745
Fragments arranged by size:
5745 1102 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6660
Fragments arranged by size:
6660 683 56
BtsI GCAGTG_nn'
Cuts at: 4915 5709 5729 4915
Size: 794 20 6585
Fragments arranged by size:
6585 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7399
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7364
DraI TTT'AAA
Cuts at: 4703 4703
Size: 7399
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3570 6406 809
Size: 507 2254 2836 1802
Fragments arranged by size:
2836 2254 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4846 5735 7364
Size: 54 1425 50 275 18 3016 889 1629
Cuts at: 7364 8
Size: 43
Fragments arranged by size:
3016 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4846 1537
Size: 275 3034 4090
Fragments arranged by size:
4090 3034 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2445 3077 5329 1353
Size: 1092 632 2252 3423
Fragments arranged by size:
3423 2252 1092 632
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5986 6360 6506 387
Size: 1064 138 4397 374 146 1280
Fragments arranged by size:
4397 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4338 4338
Size: 7399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 2504 3347 3432 3506 3617 3671
Size: 393 783 89 843 85 74 111 54
Cuts at: 3671 3732 3945 4088 5462 6059 6846 7114 1239
Size: 61 213 143 1374 597 787 268 1524
Fragments arranged by size:
1524 1374 843 787 783 597 393 268
213 143 111 89 85 74 61 54
EcoO109I rG'GnC_Cy
Cuts at: 2820 2820
Size: 7399
EcoRI G'AATT_C
Cuts at: 4708 5086 4708
Size: 378 7021
FspI TGC'GCA
Cuts at: 5905 5905
Size: 7399
HaeII r_GCGC'y
Cuts at: 1678 6546 1678
Size: 4868 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 2494 2526 2638
Size: 32 135 32 665 32 225 32 112
Cuts at: 2638 2670 2893 2925 3056 3088 4289 4321 6040
Size: 32 223 32 131 32 1201 32 1719
Cuts at: 6040 6072 6114 6146 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1719 1201 665 225 223 135 131
112 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2256 4530 4743 4989 669
Size: 988 281 318 2274 213 246 3079
Fragments arranged by size:
3079 2274 988 318 281 246 213
HindIII A'AGCT_T
Cuts at: 748 2936 4140 748
Size: 2188 1204 4007
Fragments arranged by size:
4007 2188 1204
HpaI GTT'AAC
Cuts at: 4530 4989 4530
Size: 459 6940
KpnI G_GTAC'C
Cuts at: 4724 4724
Size: 7399
MfeI C'AATT_G
Cuts at: 4998 4998
Size: 7399
MluI A'CGCG_T
Cuts at: 7275 7275
Size: 7399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3312 3761 6513 6697 1305
Size: 2007 449 2752 184 2007
Fragments arranged by size:
2752 2007 2007 449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5577
Fragments arranged by size:
5577 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2663 4469 5357 5716 5875 518
Size: 648 1497 1806 888 359 159 2042
Fragments arranged by size:
2042 1806 1497 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 2384 4815 5492 6640 6885 1606
Size: 481 297 2431 677 1148 245 2120
Fragments arranged by size:
2431 2120 1148 677 481 297 245
NaeI GCC'GGC
Cuts at: 3775 3775
Size: 7399
NcoI C'CATG_G
Cuts at: 513 1065 2167 513
Size: 552 1102 5745
Fragments arranged by size:
5745 1102 552
NdeI CA'TA_TG
Cuts at: 387 2037 3666 387
Size: 1650 1629 4120
Fragments arranged by size:
4120 1650 1629
NgoMIV G'CCGG_C
Cuts at: 3773 3773
Size: 7399
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7399
NotI GC'GGCC_GC
Cuts at: 4846 4846
Size: 7399
NsiI A_TGCA'T
Cuts at: 2435 2435
Size: 7399
NspI r_CATG'y
Cuts at: 1132 1229 4757 6302 1132
Size: 97 3528 1545 2229
Fragments arranged by size:
3528 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7399
PciI A'CATG_T
Cuts at: 6298 6298
Size: 7399
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3926 1108
Size: 2818 4581
PfoI T'CCnGG_A
Cuts at: 2541 2541
Size: 7399
PmeI GTTT'AAAC
Cuts at: 4703 4703
Size: 7399
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5468 5500 6981 7013 5468
Size: 32 1481 32 5854
Fragments arranged by size:
5854 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2820 2820
Size: 7399
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7399
PsiI TTA'TAA
Cuts at: 2198 4969 2198
Size: 2771 4628
PstI C_TGCA'G
Cuts at: 830 2473 4537 4751 830
Size: 1643 2064 214 3478
Fragments arranged by size:
3478 2064 1643 214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7399
PvuII CAG'CTG
Cuts at: 2087 2384 2087
Size: 297 7102
SacI G_AGCT'C
Cuts at: 4340 4340
Size: 7399
SalI G'TCGA_C
Cuts at: 1936 4741 1936
Size: 2805 4594
SanDI GG'GwC_CC
Cuts at: 2820 2820
Size: 7399
SapI GCTCTTCn'nnn_
Cuts at: 3077 3077
Size: 7399
SbfI CC_TGCA'GG
Cuts at: 4751 4751
Size: 7399
ScaI AGT'ACT
Cuts at: 2331 2467 2803 5647 7359 2331
Size: 136 336 2844 1712 2371
Fragments arranged by size:
2844 2371 1712 336 136
SfcI C'TryA_G
Cuts at: 826 1045 2469 3990 4132 4158 4533 4747 5882
Size: 219 1424 1521 142 26 375 214 1135
Cuts at: 5882 6563 6754 826
Size: 681 191 1471
Fragments arranged by size:
1521 1471 1424 1135 681 375 219 214
191 142 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7399
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7399
SmaI CCC'GGG
Cuts at: 1872 4726 1872
Size: 2854 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3041 3568 3599 5503 6404 6666
Size: 197 926 1098 527 31 1904 901 262
Cuts at: 6666 820
Size: 1553
Fragments arranged by size:
1904 1553 1098 926 901 527 262 197
31
SnaBI TAC'GTA
Cuts at: 493 5190 493
Size: 4697 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7399
SphI G_CATG'C
Cuts at: 1132 4757 1132
Size: 3625 3774
SspI AAT'ATT
Cuts at: 5 52 2845 3755 4678 5323 5
Size: 47 2793 910 923 645 2081
Fragments arranged by size:
2793 2081 923 910 645 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7399
StyI C'CwwG_G
Cuts at: 513 1065 2167 4838 513
Size: 552 1102 2671 3074
Fragments arranged by size:
3074 2671 1102 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5426 5443 5596 5781 6226 325
Size: 1255 3846 17 153 185 445 1498
Fragments arranged by size:
3846 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2329 2465 2801
Size: 274 80 33 51 1443 352 136 336
Cuts at: 2801 5645 7357 96
Size: 2844 1712 138
Fragments arranged by size:
2844 1712 1443 352 336 274 138 136
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 2523 2555 7359 7391 2012
Size: 32 479 32 4804 32 2020
Fragments arranged by size:
4804 2020 479 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7399
XbaI T'CTAG_A
Cuts at: 4735 4735
Size: 7399
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7399
XmaI C'CCGG_G
Cuts at: 1870 4724 1870
Size: 2854 4545
XmnI GAAnn'nnTTC
Cuts at: 2502 3200 3267 4678 5528 2502
Size: 698 67 1411 850 4373
Fragments arranged by size:
4373 1411 850 698 67
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5207 276
Size: 53 83 186 936 3673 2468
Fragments arranged by size:
3673 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI XcmI