Restriction map for
pFN21AB8590
(Circular) (Six-base) MAPSORT of: pFN21AB8590.seq Check: 2093 from: 1 to: 5740
pFN21AB8590 5740 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3803 5187 5455 1632
Size: 2171 1384 268 1917
Fragments arranged by size:
2171 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaLI G'TGCA_C
Cuts at: 2739 3797 4953 2739
Size: 1058 1156 3526
Fragments arranged by size:
3526 1156 1058
ApoI r'AATT_y
Cuts at: 1493 2092 2373 3049 3275 3427 5536 1493
Size: 599 281 676 226 152 2109 1697
Fragments arranged by size:
2109 1697 676 599 281 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 3065 1870
Size: 73 1122 4545
Fragments arranged by size:
4545 1122 73
BamHI G'GATC_C
Cuts at: 2160 3070 3437 2160
Size: 910 367 4463
Fragments arranged by size:
4463 910 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2819 3061 4516 618
Size: 325 217 627 1032 242 1455 1842
Fragments arranged by size:
1842 1455 1032 627 325 242 217
BanII G_rGCy'C
Cuts at: 1391 2561 3059 1391
Size: 1170 498 4072
Fragments arranged by size:
4072 1170 498
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3931 3965 1903
Size: 34 1994 34 3678
Fragments arranged by size:
3678 1994 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2971 3632 4842 2971
Size: 661 1210 3869
Fragments arranged by size:
3869 1210 661
BclI T'GATC_A
Cuts at: 1548 2236 1548
Size: 688 5052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4351 136
Size: 107 122 71 631 605 337 2342 1525
Fragments arranged by size:
2342 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 2743 3801 4957 1790
Size: 953 1058 1156 2573
Fragments arranged by size:
2573 1156 1058 953
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2709 3471 4429
Size: 416 53 83 399 24 1292 762 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1292 958 762 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 3042 4400 1239
Size: 1044 759 1358 2579
Fragments arranged by size:
2579 1358 1044 759
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2768 4403 882
Size: 617 1269 1635 2219
Fragments arranged by size:
2219 1635 1269 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2031 4173 4571 4845 4992 5403 2031
Size: 2142 398 274 147 411 2368
Fragments arranged by size:
2368 2142 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2395 2425 1178
Size: 30 165 30 992 30 4493
Fragments arranged by size:
4493 992 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2610 1186
Size: 1424 4316
BseYI C'CCAG_C
Cuts at: 2059 4943 5439 2059
Size: 2884 496 2360
Fragments arranged by size:
2884 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2628 2928 1163
Size: 573 892 300 3975
Fragments arranged by size:
3975 892 573 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2743 3801 3886 4957 2743
Size: 1058 85 1071 3526
Fragments arranged by size:
3526 1071 1058 85
BsmI GAATG_Cn'
Cuts at: 2532 3251 3344 2532
Size: 719 93 4928
Fragments arranged by size:
4928 719 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2561 2743 3059 3801 3886 4957 1391
Size: 399 771 182 316 742 85 1071 2174
Fragments arranged by size:
2174 1071 771 742 399 316 182 85
BspEI T'CCGG_A
Cuts at: 2031 5403 2031
Size: 3372 2368
BspHI T'CATG_A
Cuts at: 2457 2466 3629 5359 2457
Size: 9 1163 1730 2838
Fragments arranged by size:
2838 1730 1163 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3095 844
Size: 2251 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2847 4235 4409 65
Size: 1183 1599 1388 174 1396
Fragments arranged by size:
1599 1396 1388 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5740
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2160 2786 3070 3437 3822 3839 5291 5377
Size: 188 626 284 367 385 17 1452 86
Cuts at: 5377 5389 5735 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 626 385 367 346 284 188
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2511 2811 513
Size: 552 1446 300 3442
Fragments arranged by size:
3442 1446 552 300
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 2616 3256 4050 4070 2616
Size: 640 794 20 4286
Fragments arranged by size:
4286 794 640 20
ClaI AT'CG_AT
Cuts at: 1554 2213 1554
Size: 659 5081
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 2230 3044 2230
Size: 814 4926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2179 2700 3187
Size: 54 1425 50 275 18 349 521 487
Cuts at: 3187 4076 5705 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 521 487 349 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2649 3670 1353
Size: 1296 1021 3423
Fragments arranged by size:
3423 1296 1021
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 3042 3803 4400 5187 5455 1239
Size: 393 651 759 761 597 787 268 1524
Fragments arranged by size:
1524 787 761 759 651 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2639 2639
Size: 5740
EcoRI G'AATT_C
Cuts at: 2092 2373 3049 3427 2092
Size: 281 676 378 4405
Fragments arranged by size:
4405 676 378 281
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4381 4413 4455 4487 1373
Size: 32 135 32 2809 32 42 32 2626
Fragments arranged by size:
2809 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2121 3084 3330 669
Size: 988 281 183 963 246 3079
Fragments arranged by size:
3079 988 963 281 246 183
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 2121 3330 2121
Size: 1209 4531
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 2490 3339 2490
Size: 849 4891
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2497 4854 5038 1305
Size: 1192 2357 184 2007
Fragments arranged by size:
2357 2007 1192 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2181 2702 10
Size: 54 1425 343 349 521 3048
Fragments arranged by size:
3048 1425 521 349 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 2595 3698 4057 4216 518
Size: 648 1221 208 1103 359 159 2042
Fragments arranged by size:
2042 1221 1103 648 359 208 159
MspA1I CmG'CkG
Cuts at: 1606 2672 3156 3833 4981 5226 1606
Size: 1066 484 677 1148 245 2120
Fragments arranged by size:
2120 1148 1066 677 484 245
NcoI C'CATG_G
Cuts at: 513 1065 2511 513
Size: 552 1446 3742
Fragments arranged by size:
3742 1446 552
NdeI CA'TA_TG
Cuts at: 387 2548 387
Size: 2161 3579
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 2872 3098 4643 1132
Size: 97 1643 226 1545 2229
Fragments arranged by size:
2229 1643 1545 226 97
PasI CC'CwG_GG
Cuts at: 1348 2746 1348
Size: 1398 4342
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2927 1108
Size: 1819 3921
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2639 2639
Size: 5740
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 3092 830
Size: 2262 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2672 2672
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 3088 4223 4904 5095 826
Size: 219 2043 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3844 4745 5007 820
Size: 197 926 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 2631 3664 5
Size: 47 2579 1033 2081
Fragments arranged by size:
2579 2081 1033 47
StuI AGG'CCT
Cuts at: 1517 2075 2688 1517
Size: 558 613 4569
Fragments arranged by size:
4569 613 558
StyI C'CwwG_G
Cuts at: 513 1065 2070 2511 3179 513
Size: 552 1005 441 668 3074
Fragments arranged by size:
3074 1005 668 552 441
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2073 2473 2594 2726 3767 3784 3937
Size: 1255 493 400 121 132 1041 17 153
Cuts at: 3937 4122 4567 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1041 493 445 400 185 153
132 121 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 3986 5698
Size: 274 80 33 51 1443 414 1595 1712
Cuts at: 5698 96
Size: 138
Fragments arranged by size:
1712 1595 1443 414 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2152 2184 5700 5732 2152
Size: 32 3516 32 2160
Fragments arranged by size:
3516 2160 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 2377 2724 3869 2377
Size: 347 1145 4248
Fragments arranged by size:
4248 1145 347
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI