Restriction map for
pFN21AB8589
(Circular) (Six-base) MAPSORT of: pFN21AB8589.seq Check: 2899 from: 1 to: 5779
pFN21AB8589 5779 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2488 3589 278
Size: 53 83 186 936 952 1101 2468
Fragments arranged by size:
2468 1101 952 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2224 3122 1937
Size: 287 898 4594
Fragments arranged by size:
4594 898 287
Acc65I G'GTAC_C
Cuts at: 2833 3100 2833
Size: 267 5512
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2808 3093 3842 5226 5494 1632
Size: 1176 285 749 1384 268 1917
Fragments arranged by size:
1917 1384 1176 749 285 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 2129 4678 5655 1203
Size: 926 2549 977 1327
Fragments arranged by size:
2549 1327 977 926
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2619 2651 2619
Size: 32 5747
AlwNI CAG_nnn'CTG
Cuts at: 3071 3522 5094 3071
Size: 451 1572 3756
Fragments arranged by size:
3756 1572 451
ApaLI G'TGCA_C
Cuts at: 3061 3836 4992 3061
Size: 775 1156 3848
Fragments arranged by size:
3848 1156 775
ApoI r'AATT_y
Cuts at: 1493 2232 3088 3314 3466 5575 1493
Size: 739 856 226 152 2109 1697
Fragments arranged by size:
2109 1697 856 739 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2237 3104 1870
Size: 73 294 867 4545
Fragments arranged by size:
4545 867 294 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2833 3100 4555 618
Size: 325 217 627 1046 267 1455 1842
Fragments arranged by size:
1842 1455 1046 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2591 3098 1391
Size: 1200 507 4072
Fragments arranged by size:
4072 1200 507
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2643 2739 928
Size: 971 744 96 3968
Fragments arranged by size:
3968 971 744 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3970 4004 1903
Size: 34 2033 34 3678
Fragments arranged by size:
3678 2033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 2026 1548
Size: 478 5301
BfrBI ATG'CAT
Cuts at: 2315 2315
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4390 136
Size: 107 122 71 631 605 2718 1525
Fragments arranged by size:
2718 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3038 5774 3038
Size: 2736 3043
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 3065 3840 4996 1790
Size: 1275 775 1156 2573
Fragments arranged by size:
2573 1275 1156 775
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4468 442
Size: 416 53 83 399 24 2093 958 1753
Fragments arranged by size:
2093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4439 1239
Size: 3200 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 3904 4769 5067 2267
Size: 1637 865 298 2979
Fragments arranged by size:
2979 1637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3570 493
Size: 711 2366 2702
Fragments arranged by size:
2702 2366 711
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2485 3586 3968 4603
Size: 53 83 186 936 952 1101 382 635
Cuts at: 4603 275
Size: 1451
Fragments arranged by size:
1451 1101 952 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2619 2649 1178
Size: 30 165 30 1216 30 4308
Fragments arranged by size:
4308 1216 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5779
BseYI C'CCAG_C
Cuts at: 4982 5478 4982
Size: 496 5283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2070 1163
Size: 573 334 4872
Fragments arranged by size:
4872 573 334
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2974 2987 3229 3990 5018 1540
Size: 275 186 973 13 242 761 1028 2301
Fragments arranged by size:
2301 1028 973 761 275 242 186 13
BsiHKAI G_wGCw'C
Cuts at: 2218 2591 3065 3840 3925 4996 2218
Size: 373 474 775 85 1071 3001
Fragments arranged by size:
3001 1071 775 474 373 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2218 2591 3065 3098 3840 3925 4996
Size: 399 428 373 474 33 742 85 1071
Cuts at: 4996 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 474 428 399 373 85
33
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 2809 3668 5398 2809
Size: 859 1730 3190
Fragments arranged by size:
3190 1730 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3134 844
Size: 2290 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2603 3666 1394
Size: 429 780 1063 3507
Fragments arranged by size:
3507 1063 780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4423 4610 1700
Size: 110 2613 187 2869
Fragments arranged by size:
2869 2613 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2909 794
Size: 2115 3664
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 3038 3109 3476 3861 3878 5330 5416 5428
Size: 1066 71 367 385 17 1452 86 12
Cuts at: 5428 5774 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1066 385 367 346 86 71
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4288
Fragments arranged by size:
4288 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 3295 4089 4109 3295
Size: 794 20 4965
Fragments arranged by size:
4965 794 20
ClaI AT'CG_AT
Cuts at: 1554 2974 2983 2998 1554
Size: 1420 9 15 4335
Fragments arranged by size:
4335 1420 15 9
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DraIII CAC_nnn'GTG
Cuts at: 2624 2624
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3226 4115 5744
Size: 54 1425 50 275 18 1396 889 1629
Cuts at: 5744 8
Size: 43
Fragments arranged by size:
1629 1425 1396 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3226 1537
Size: 275 1414 4090
Fragments arranged by size:
4090 1414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3709 1353
Size: 2356 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4366 4740 4886 387
Size: 1064 138 2777 374 146 1280
Fragments arranged by size:
2777 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2589 2589
Size: 5779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2808 3093 3842 4439 5226 5494 1239
Size: 393 1176 285 749 597 787 268 1524
Fragments arranged by size:
1524 1176 787 749 597 393 285 268
EcoO109I rG'GnC_Cy
Cuts at: 2661 2661
Size: 5779
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 5779
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 4926 1678
Size: 3248 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 2798 2830 2882
Size: 32 135 32 1025 32 169 32 52
Cuts at: 2882 2914 4420 4452 4494 4526 1373
Size: 32 1506 32 42 32 2626
Fragments arranged by size:
2626 1506 1025 169 135 52 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2225 2688 3123 3369 669
Size: 988 281 287 463 435 246 3079
Fragments arranged by size:
3079 988 463 435 287 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 2688 3369 2688
Size: 681 5098
KpnI G_GTAC'C
Cuts at: 2837 3104 2837
Size: 267 5512
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4893 5077 1305
Size: 3588 184 2007
Fragments arranged by size:
3588 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3957
Fragments arranged by size:
3957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 3737 4096 4255 518
Size: 648 1245 1326 359 159 2042
Fragments arranged by size:
2042 1326 1245 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2774 3195 3872 5020 5265 1606
Size: 1168 421 677 1148 245 2120
Fragments arranged by size:
2120 1168 1148 677 421 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4288
Fragments arranged by size:
4288 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NsiI A_TGCA'T
Cuts at: 2317 2317
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2044 2133 3137 4682 1132
Size: 97 815 89 1004 1545 2229
Fragments arranged by size:
2229 1545 1004 815 97 89
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 2129 4678 2129
Size: 2549 3230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2619 2651 3848 3880 5361 5393 2619
Size: 32 1197 32 1481 32 3005
Fragments arranged by size:
3005 1481 1197 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2905 3131 830
Size: 2075 226 3478
Fragments arranged by size:
3478 2075 226
PvuI CG_AT'CG
Cuts at: 2001 2974 2987 2001
Size: 973 13 4793
Fragments arranged by size:
4793 973 13
PvuII CAG'CTG
Cuts at: 2774 2774
Size: 5779
SacI G_AGCT'C
Cuts at: 2591 2591
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 2223 3121 1936
Size: 287 898 4594
Fragments arranged by size:
4594 898 287
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2901 3127 4262 4943 5134 826
Size: 219 1856 226 1135 681 191 1471
Fragments arranged by size:
1856 1471 1135 681 226 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 3883 4784 5046 820
Size: 197 926 339 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 339 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 2363 152
Size: 2211 3568
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 3049 1517
Size: 1532 4247
StyI C'CwwG_G
Cuts at: 513 1065 2004 3218 513
Size: 552 939 1214 3074
Fragments arranged by size:
3074 1214 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2328 3806 3823 3976 4161 4606 325
Size: 1255 748 1478 17 153 185 445 1498
Fragments arranged by size:
1498 1478 1255 748 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4025 5737 96
Size: 274 80 33 51 1443 2048 1712 138
Fragments arranged by size:
2048 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5739 5771 5739
Size: 32 5747
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3041 3115 3041
Size: 74 5705
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2486 3587 276
Size: 53 83 186 936 952 1101 2468
Fragments arranged by size:
2468 1101 952 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI