Restriction map for
pFN21AB8587
(Circular) (Six-base) MAPSORT of: pFN21AB8587.seq Check: 8057 from: 1 to: 5416
pFN21AB8587 5416 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3226 278
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 1937
Size: 822 4594
Acc65I G'GTAC_C
Cuts at: 2737 2737
Size: 5416
AclI AA'CG_TT
Cuts at: 1563 3543 3916 1563
Size: 1980 373 3063
Fragments arranged by size:
3063 1980 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 2609 3479 4863 5131 1632
Size: 621 356 870 1384 268 1917
Fragments arranged by size:
1917 1384 870 621 356 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5219
AflIII A'CryG_T
Cuts at: 1203 4315 5292 1203
Size: 3112 977 1327
Fragments arranged by size:
3112 1327 977
AgeI A'CCGG_T
Cuts at: 4247 4247
Size: 5416
AhdI GACnn_n'nnGTC
Cuts at: 2281 4145 2281
Size: 1864 3552
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5416
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2418 2450 2418
Size: 32 5384
AlwNI CAG_nnn'CTG
Cuts at: 2538 3159 4731 2538
Size: 621 1572 3223
Fragments arranged by size:
3223 1572 621
ApaI G_GGCC'C
Cuts at: 2074 2074
Size: 5416
ApaLI G'TGCA_C
Cuts at: 3473 4629 3473
Size: 1156 4260
ApoI r'AATT_y
Cuts at: 1493 2372 2428 2558 2725 2951 3103 5212 1493
Size: 879 56 130 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 879 226 167 152 130 56
AseI AT'TA_AT
Cuts at: 160 1032 3970 160
Size: 872 2938 1606
Fragments arranged by size:
2938 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2741 1870
Size: 73 130 668 4545
Fragments arranged by size:
4545 668 130 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2525 2558 2525
Size: 33 5383
BamHI G'GATC_C
Cuts at: 2746 3113 2746
Size: 367 5049
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2737 4192 618
Size: 325 217 627 950 1455 1842
Fragments arranged by size:
1842 1455 950 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2027 2074 2412 2735 1391
Size: 636 47 338 323 4072
Fragments arranged by size:
4072 636 338 323 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2711 928
Size: 971 812 3633
Fragments arranged by size:
3633 971 812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3607 3641 1903
Size: 34 50 34 1586 34 3678
Fragments arranged by size:
3678 1586 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2553 3308 4518 2553
Size: 755 1210 3451
Fragments arranged by size:
3451 1210 755
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 4027 136
Size: 107 122 71 631 605 433 1922 1525
Fragments arranged by size:
1922 1525 631 605 433 122 107 71
BglII A'GATC_T
Cuts at: 5411 5411
Size: 5416
BlpI GC'TnA_GC
Cuts at: 2124 2833 2124
Size: 709 4707
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2593 3477 4633 1790
Size: 284 519 884 1156 2573
Fragments arranged by size:
2573 1156 884 519 284
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5416
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2467 3147 4105
Size: 416 53 83 399 24 1050 680 958
Cuts at: 4105 442
Size: 1753
Fragments arranged by size:
1753 1050 958 680 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5416
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2139 2139
Size: 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2011 2168 2388 4076 1239
Size: 772 157 220 1688 2579
Fragments arranged by size:
2579 1688 772 220 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3541 4406 4704 3541
Size: 865 298 4253
Fragments arranged by size:
4253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4079 882
Size: 617 2580 2219
Fragments arranged by size:
2580 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3207 493
Size: 711 2003 2702
Fragments arranged by size:
2702 2003 711
BsaBI GATnn'nnATC
Cuts at: 5410 5410
Size: 5416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3223 3605 4240 275
Size: 53 83 186 936 1690 382 635 1451
Fragments arranged by size:
1690 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3849 4247 4521 4668 5079 3849
Size: 398 274 147 411 4186
Fragments arranged by size:
4186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5191
Fragments arranged by size:
5191 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2391 1186
Size: 1205 4211
BseYI C'CCAG_C
Cuts at: 4619 5115 4619
Size: 496 4920
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2551 1163
Size: 573 815 4028
Fragments arranged by size:
4028 815 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2866 3627 4655 1540
Size: 275 186 865 761 1028 2301
Fragments arranged by size:
2301 1028 865 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2516 3477 3562 4633 2516
Size: 961 85 1071 3299
Fragments arranged by size:
3299 1071 961 85
BsmI GAATG_Cn'
Cuts at: 2927 3020 2927
Size: 93 5323
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2074 2412 2516 2593 2735 3477
Size: 399 237 47 338 104 77 142 742
Cuts at: 3477 3562 4633 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 338 237 142 104
85 77 47
BspEI T'CCGG_A
Cuts at: 5079 5079
Size: 5416
BspHI T'CATG_A
Cuts at: 2437 2478 3305 5035 2437
Size: 41 827 1730 2818
Fragments arranged by size:
2818 1730 827 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2771 844
Size: 1927 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3303 1394
Size: 429 1480 3507
Fragments arranged by size:
3507 1480 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2351 3911 4085 65
Size: 1183 1103 1560 174 1396
Fragments arranged by size:
1560 1396 1183 1103 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4060 4247 1700
Size: 110 2250 187 2869
Fragments arranged by size:
2869 2250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5416
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5416
BssSI C'ACGA_G
Cuts at: 3476 4488 3476
Size: 1012 4404
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5416
BstBI TT'CG_AA
Cuts at: 4220 4220
Size: 5416
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5416
BstYI r'GATC_y
Cuts at: 1972 2746 3113 3498 3515 4967 5053 5065 5411
Size: 774 367 385 17 1452 86 12 346
Cuts at: 5411 1972
Size: 1977
Fragments arranged by size:
1977 1452 774 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2100 513
Size: 552 1035 3829
Fragments arranged by size:
3829 1035 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4677
Fragments arranged by size:
4677 683 56
BtsI GCAGTG_nn'
Cuts at: 2337 2932 3726 3746 2337
Size: 595 794 20 4007
Fragments arranged by size:
4007 794 595 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5381
DraI TTT'AAA
Cuts at: 2720 2720
Size: 5416
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4423 809
Size: 507 3107 1802
Fragments arranged by size:
3107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2863 3752 5381
Size: 54 1425 50 275 18 1033 889 1629
Cuts at: 5381 8
Size: 43
Fragments arranged by size:
1629 1425 1033 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2863 1537
Size: 275 1051 4090
Fragments arranged by size:
4090 1051 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 3346 1353
Size: 1313 680 3423
Fragments arranged by size:
3423 1313 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4003 4377 4523 387
Size: 1064 138 2414 374 146 1280
Fragments arranged by size:
2414 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2011 2168 2253 2388 2609 3479 4076
Size: 393 379 157 85 135 221 870 597
Cuts at: 4076 4863 5131 1239
Size: 787 268 1524
Fragments arranged by size:
1524 870 787 597 393 379 268 221
157 135 85
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 5416
EcoO109I rG'GnC_Cy
Cuts at: 2070 2237 2070
Size: 167 5249
EcoRI G'AATT_C
Cuts at: 2725 3103 2725
Size: 378 5038
FspI TGC'GCA
Cuts at: 3922 3922
Size: 5416
HaeII r_GCGC'y
Cuts at: 1678 4563 1678
Size: 2885 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2182 2214 2267 2299 4057
Size: 32 135 32 610 32 53 32 1758
Cuts at: 4057 4089 4131 4163 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1758 610 135 53 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2760 3006 669
Size: 988 281 822 246 3079
Fragments arranged by size:
3079 988 822 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5416
HpaI GTT'AAC
Cuts at: 3006 3006
Size: 5416
KpnI G_GTAC'C
Cuts at: 2741 2741
Size: 5416
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 5416
MluI A'CGCG_T
Cuts at: 5292 5292
Size: 5416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4530 4714 1305
Size: 3225 184 2007
Fragments arranged by size:
3225 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3594
Fragments arranged by size:
3594 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3374 3733 3892 518
Size: 648 2208 359 159 2042
Fragments arranged by size:
2208 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2832 3509 4657 4902 1606
Size: 522 704 677 1148 245 2120
Fragments arranged by size:
2120 1148 704 677 522 245
NcoI C'CATG_G
Cuts at: 513 1065 2100 513
Size: 552 1035 3829
Fragments arranged by size:
3829 1035 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5416
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5416
NotI GC'GGCC_GC
Cuts at: 2863 2863
Size: 5416
NspI r_CATG'y
Cuts at: 1132 1229 2284 2470 2774 4319 1132
Size: 97 1055 186 304 1545 2229
Fragments arranged by size:
2229 1545 1055 304 186 97
PasI CC'CwG_GG
Cuts at: 1348 2020 1348
Size: 672 4744
PciI A'CATG_T
Cuts at: 4315 4315
Size: 5416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5416
PmeI GTTT'AAAC
Cuts at: 2720 2720
Size: 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 4998 5030 3485
Size: 32 1481 32 3871
Fragments arranged by size:
3871 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2237 2237
Size: 5416
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5416
PsiI TTA'TAA
Cuts at: 2252 2986 2252
Size: 734 4682
PspOMI G'GGCC_C
Cuts at: 2070 2070
Size: 5416
PstI C_TGCA'G
Cuts at: 830 2768 830
Size: 1938 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5416
SalI G'TCGA_C
Cuts at: 1936 2758 1936
Size: 822 4594
SbfI CC_TGCA'GG
Cuts at: 2768 2768
Size: 5416
ScaI AGT'ACT
Cuts at: 3664 5376 3664
Size: 1712 3704
SfcI C'TryA_G
Cuts at: 826 1045 2764 3899 4580 4771 826
Size: 219 1719 1135 681 191 1471
Fragments arranged by size:
1719 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5416
SmaI CCC'GGG
Cuts at: 1872 2743 1872
Size: 871 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3520 4421 4683 820
Size: 197 926 1577 901 262 1553
Fragments arranged by size:
1577 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3207 493
Size: 2714 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5416
SphI G_CATG'C
Cuts at: 1132 2774 1132
Size: 1642 3774
SspI AAT'ATT
Cuts at: 5 52 3340 5
Size: 47 3288 2081
Fragments arranged by size:
3288 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5416
StyI C'CwwG_G
Cuts at: 513 1065 2100 2325 2855 513
Size: 552 1035 225 530 3074
Fragments arranged by size:
3074 1035 552 530 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2128 3443 3460 3613 3798 4243 325
Size: 1255 548 1315 17 153 185 445 1498
Fragments arranged by size:
1498 1315 1255 548 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2342 3662 5374
Size: 274 80 33 51 1443 365 1320 1712
Cuts at: 5374 96
Size: 138
Fragments arranged by size:
1712 1443 1320 365 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5376 5408 5376
Size: 32 5384
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5416
XbaI T'CTAG_A
Cuts at: 2752 2752
Size: 5416
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5416
XmaI C'CCGG_G
Cuts at: 1870 2741 1870
Size: 871 4545
XmnI GAAnn'nnTTC
Cuts at: 3545 3545
Size: 5416
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3224 276
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI