Restriction map for
pFN21AB8584
(Circular) (Six-base) MAPSORT of: pFN21AB8584.seq Check: 4202 from: 1 to: 7024
pFN21AB8584 7024 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3360 4834 278
Size: 53 83 186 936 1824 1474 2468
Fragments arranged by size:
2468 1824 1474 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2271 4367 1937
Size: 334 2096 4594
Fragments arranged by size:
4594 2096 334
Acc65I G'GTAC_C
Cuts at: 4345 4345
Size: 7024
AclI AA'CG_TT
Cuts at: 1563 2769 5151 5524 1563
Size: 1206 2382 373 3063
Fragments arranged by size:
3063 2382 1206 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3215 3398 3491 3740 4014 5087 6471 6739
Size: 1583 183 93 249 274 1073 1384 268
Cuts at: 6739 1632
Size: 1917
Fragments arranged by size:
1917 1583 1384 1073 274 268 249 183
93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6827
AflIII A'CryG_T
Cuts at: 1203 2775 5923 6900 1203
Size: 1572 3148 977 1327
Fragments arranged by size:
3148 1572 1327 977
AgeI A'CCGG_T
Cuts at: 5855 5855
Size: 7024
AhdI GACnn_n'nnGTC
Cuts at: 5753 5753
Size: 7024
AleI CACnn'nnGTG
Cuts at: 1166 2463 1166
Size: 1297 5727
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7024
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 6992
AlwNI CAG_nnn'CTG
Cuts at: 2545 3124 3448 3703 3718 4767 6339 2545
Size: 579 324 255 15 1049 1572 3230
Fragments arranged by size:
3230 1572 1049 579 324 255 15
ApaI G_GGCC'C
Cuts at: 2960 3948 2960
Size: 988 6036
ApaLI G'TGCA_C
Cuts at: 5081 6237 5081
Size: 1156 5868
ApoI r'AATT_y
Cuts at: 1493 2291 2324 2390 4333 4559 4711 6820 1493
Size: 798 33 66 1943 226 152 2109 1697
Fragments arranged by size:
2109 1943 1697 798 226 152 66 33
AseI AT'TA_AT
Cuts at: 160 1032 5578 160
Size: 872 4546 1606
Fragments arranged by size:
4546 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
AvaI C'yCGr_G
Cuts at: 1870 1943 4349 1870
Size: 73 2406 4545
Fragments arranged by size:
4545 2406 73
BamHI G'GATC_C
Cuts at: 3019 3028 4354 4721 3019
Size: 9 1326 367 5322
Fragments arranged by size:
5322 1326 367 9
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4148 4345 5800 618
Size: 325 217 627 2361 197 1455 1842
Fragments arranged by size:
2361 1842 1455 627 325 217 197
BanII G_rGCy'C
Cuts at: 1391 2631 2960 3948 4343 1391
Size: 1240 329 988 395 4072
Fragments arranged by size:
4072 1240 988 395 329
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6053
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2846 2880 5215 5249 1903
Size: 34 909 34 2335 34 3678
Fragments arranged by size:
3678 2335 909 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2818 4916 6126 2818
Size: 2098 1210 3716
Fragments arranged by size:
3716 2098 1210
BclI T'GATC_A
Cuts at: 1548 2163 1548
Size: 615 6409
BfrBI ATG'CAT
Cuts at: 3206 3206
Size: 7024
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5635 136
Size: 107 122 71 631 605 3963 1525
Fragments arranged by size:
3963 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4265 7019 4265
Size: 2754 4270
BlpI GC'TnA_GC
Cuts at: 3072 4441 3072
Size: 1369 5655
Bme1580I G_kGCm'C
Cuts at: 1790 2960 3297 3792 3948 4151 5085 6241 1790
Size: 1170 337 495 156 203 934 1156 2573
Fragments arranged by size:
2573 1170 1156 934 495 337 203 156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7024
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2900 2981 3414
Size: 416 53 83 399 24 1483 81 433
Cuts at: 3414 4755 5713 442
Size: 1341 958 1753
Fragments arranged by size:
1753 1483 1341 958 433 416 399 83
81 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7024
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2791 2826 2936 4073 5684 1239
Size: 1552 35 110 1137 1611 2579
Fragments arranged by size:
2579 1611 1552 1137 110 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2625 5149 6014 6312 2625
Size: 2524 865 298 3337
Fragments arranged by size:
3337 2524 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4049 5687 882
Size: 617 2550 1638 2219
Fragments arranged by size:
2550 2219 1638 617
BsaAI yAC'GTr
Cuts at: 493 1204 3776 4815 493
Size: 711 2572 1039 2702
Fragments arranged by size:
2702 2572 1039 711
BsaBI GATnn'nnATC
Cuts at: 7018 7018
Size: 7024
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3357 4831 5213 5848
Size: 53 83 186 936 1824 1474 382 635
Cuts at: 5848 275
Size: 1451
Fragments arranged by size:
1824 1474 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5457 5855 6129 6276 6687 5457
Size: 398 274 147 411 5794
Fragments arranged by size:
5794 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6799
Fragments arranged by size:
6799 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3695 3698 1186
Size: 2509 3 4512
Fragments arranged by size:
4512 2509 3
BseYI C'CCAG_C
Cuts at: 3320 6227 6723 3320
Size: 2907 496 3621
Fragments arranged by size:
3621 2907 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3473 3551 3773 4139 1163
Size: 573 1737 78 222 366 4048
Fragments arranged by size:
4048 1737 573 366 222 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4474 5235 6263 1540
Size: 275 186 2473 761 1028 2301
Fragments arranged by size:
2473 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2153 2631 3073 5085 5170 6241 2153
Size: 478 442 2012 85 1071 2936
Fragments arranged by size:
2936 2012 1071 478 442 85
BsmI GAATG_Cn'
Cuts at: 2526 3208 4008 4535 4628 2526
Size: 682 800 527 93 4922
Fragments arranged by size:
4922 800 682 527 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2631 2960 3073 3297 3792 3948
Size: 399 363 478 329 113 224 495 156
Cuts at: 3948 4151 4343 5085 5170 6241 1391
Size: 203 192 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 495 478 399 363 329
224 203 192 156 113 85
BspEI T'CCGG_A
Cuts at: 6687 6687
Size: 7024
BspHI T'CATG_A
Cuts at: 4913 6643 4913
Size: 1730 5294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4379 844
Size: 3535 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3640 4911 1394
Size: 429 1817 1271 3507
Fragments arranged by size:
3507 1817 1271 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5519 5693 65
Size: 1183 4271 174 1396
Fragments arranged by size:
4271 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5668 5855 1700
Size: 110 3858 187 2869
Fragments arranged by size:
3858 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2892 96
Size: 2796 4228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7024
BssSI C'ACGA_G
Cuts at: 5084 6096 5084
Size: 1012 6012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3451 3580 3703 794
Size: 2657 129 123 4115
Fragments arranged by size:
4115 2657 129 123
BstBI TT'CG_AA
Cuts at: 5828 5828
Size: 7024
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7024
BstYI r'GATC_y
Cuts at: 1972 3019 3028 4265 4354 4721 5106 5123 6575
Size: 1047 9 1237 89 367 385 17 1452
Cuts at: 6575 6661 6673 7019 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1237 1047 385 367 346 89
86 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 2490 2877 3930 2490
Size: 387 1053 5584
Fragments arranged by size:
5584 1053 387
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4152 487
Size: 683 56 2926 3359
Fragments arranged by size:
3359 2926 683 56
BtsI GCAGTG_nn'
Cuts at: 3297 4540 5334 5354 3297
Size: 1243 794 20 4967
Fragments arranged by size:
4967 1243 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6989
DraI TTT'AAA
Cuts at: 2340 4328 2340
Size: 1988 5036
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6031 809
Size: 507 4715 1802
Fragments arranged by size:
4715 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4159 4471 5360
Size: 54 1425 50 275 18 2329 312 889
Cuts at: 5360 6989 8
Size: 1629 43
Fragments arranged by size:
2329 1629 1425 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4471 1537
Size: 275 2659 4090
Fragments arranged by size:
4090 2659 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2023 2194 2199 2250 3153 4954 1353
Size: 670 171 5 51 903 1801 3423
Fragments arranged by size:
3423 1801 903 670 171 51 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5611 5985 6131 387
Size: 1064 138 4022 374 146 1280
Fragments arranged by size:
4022 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2629 2629
Size: 7024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2791 2826 2936 3215 3398 3491 3740
Size: 393 1159 35 110 279 183 93 249
Cuts at: 3740 4014 4073 5087 5684 6471 6739 1239
Size: 274 59 1014 597 787 268 1524
Fragments arranged by size:
1524 1159 1014 787 597 393 279 274
268 249 183 110 93 59 35
EcoO109I rG'GnC_Cy
Cuts at: 2956 2974 3944 3945 4208 4235 2956
Size: 18 970 1 263 27 5745
Fragments arranged by size:
5745 970 263 27 18 1
EcoRI G'AATT_C
Cuts at: 4333 4711 4333
Size: 378 6646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2606 2606
Size: 7024
FspI TGC'GCA
Cuts at: 5530 5530
Size: 7024
HaeII r_GCGC'y
Cuts at: 1678 6171 1678
Size: 4493 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2337 2369 3208
Size: 32 135 32 450 32 283 32 839
Cuts at: 3208 3240 3263 3295 5665 5697 5739 5771 1373
Size: 32 23 32 2370 32 42 32 2626
Fragments arranged by size:
2626 2370 839 450 283 135 42 32
32 32 32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 4368 4614 669
Size: 988 281 2430 246 3079
Fragments arranged by size:
3079 2430 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7024
HpaI GTT'AAC
Cuts at: 4614 4614
Size: 7024
KpnI G_GTAC'C
Cuts at: 4349 4349
Size: 7024
MfeI C'AATT_G
Cuts at: 4623 4623
Size: 7024
MluI A'CGCG_T
Cuts at: 6900 6900
Size: 7024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2979 3411 6138 6322 1305
Size: 1674 432 2727 184 2007
Fragments arranged by size:
2727 2007 1674 432 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5202
Fragments arranged by size:
5202 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2171 2463 2663 4982 5341 5500
Size: 648 843 162 292 200 2319 359 159
Cuts at: 5500 518
Size: 2042
Fragments arranged by size:
2319 2042 843 648 359 292 200 162
159
MspA1I CmG'CkG
Cuts at: 1606 2213 2690 3484 3490 3502 3511 3703 3854
Size: 607 477 794 6 12 9 192 151
Cuts at: 3854 3961 4440 5117 6265 6510 1606
Size: 107 479 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 607 479 477 245
192 151 107 12 9 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6472
NdeI CA'TA_TG
Cuts at: 387 3103 387
Size: 2716 4308
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7024
NotI GC'GGCC_GC
Cuts at: 4471 4471
Size: 7024
NsiI A_TGCA'T
Cuts at: 3208 3208
Size: 7024
NspI r_CATG'y
Cuts at: 1132 1229 2484 2779 4382 5927 1132
Size: 97 1255 295 1603 1545 2229
Fragments arranged by size:
2229 1603 1545 1255 295 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7024
PciI A'CATG_T
Cuts at: 2775 5923 2775
Size: 3148 3876
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3124 1108
Size: 2016 5008
PfoI T'CCnGG_A
Cuts at: 2820 2820
Size: 7024
PmeI GTTT'AAAC
Cuts at: 4328 4328
Size: 7024
PmlI CAC'GTG
Cuts at: 3776 3776
Size: 7024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5093 5125 6606 6638 5093
Size: 32 1481 32 5479
Fragments arranged by size:
5479 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2974 4208 2974
Size: 1234 5790
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7024
PsiI TTA'TAA
Cuts at: 4594 4594
Size: 7024
PspOMI G'GGCC_C
Cuts at: 2956 3944 2956
Size: 988 6036
PstI C_TGCA'G
Cuts at: 830 2550 3570 3603 4297 4376 830
Size: 1720 1020 33 694 79 3478
Fragments arranged by size:
3478 1720 1020 694 79 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7024
PvuII CAG'CTG
Cuts at: 2213 3490 3502 3511 3703 3854 3961 2213
Size: 1277 12 9 192 151 107 5276
Fragments arranged by size:
5276 1277 192 151 107 12 9
SacI G_AGCT'C
Cuts at: 2631 2631
Size: 7024
SalI G'TCGA_C
Cuts at: 1936 4366 1936
Size: 2430 4594
SbfI CC_TGCA'GG
Cuts at: 4376 4376
Size: 7024
ScaI AGT'ACT
Cuts at: 5272 6984 5272
Size: 1712 5312
SfcI C'TryA_G
Cuts at: 826 1045 2117 2546 3566 3599 3632 3665 4293
Size: 219 1072 429 1020 33 33 33 628
Cuts at: 4293 4372 5507 6188 6379 826
Size: 79 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 1020 681 628 429 219
191 79 33 33 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7024
SmaI CCC'GGG
Cuts at: 1872 4351 1872
Size: 2479 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2640 5128 6029 6291 820
Size: 197 926 697 2488 901 262 1553
Fragments arranged by size:
2488 1553 926 901 697 262 197
SnaBI TAC'GTA
Cuts at: 493 4815 493
Size: 4322 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7024
SphI G_CATG'C
Cuts at: 1132 4382 1132
Size: 3250 3774
SspI AAT'ATT
Cuts at: 5 52 4948 5
Size: 47 4896 2081
Fragments arranged by size:
4896 2081 47
StuI AGG'CCT
Cuts at: 1517 3012 1517
Size: 1495 5529
StyI C'CwwG_G
Cuts at: 513 1065 3351 4231 4463 513
Size: 552 2286 880 232 3074
Fragments arranged by size:
3074 2286 880 552 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4094 5051 5068 5221 5406 5851 325
Size: 1255 2514 957 17 153 185 445 1498
Fragments arranged by size:
2514 1498 1255 957 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2587 2810 2892
Size: 274 80 33 51 1443 610 223 82
Cuts at: 2892 5270 6982 96
Size: 2378 1712 138
Fragments arranged by size:
2378 1712 1443 610 274 223 138 82
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3347 3379 4212 4244 6984 7016 3347
Size: 32 833 32 2740 32 3355
Fragments arranged by size:
3355 2740 833 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2036 1316
Size: 720 6304
XbaI T'CTAG_A
Cuts at: 4360 4360
Size: 7024
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4048 4048
Size: 7024
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7024
XmaI C'CCGG_G
Cuts at: 1870 4349 1870
Size: 2479 4545
XmnI GAAnn'nnTTC
Cuts at: 2769 5153 2769
Size: 2384 4640
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3358 4832 276
Size: 53 83 186 936 1824 1474 2468
Fragments arranged by size:
2468 1824 1474 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI