Restriction map for
pFN21AB8582
(Circular) (Six-base) MAPSORT of: pFN21AB8582.seq Check: 9239 from: 1 to: 6952
pFN21AB8582 6952 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3174 3700 3174
Size: 526 6426
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4762 278
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3300 3462 4295 1937
Size: 1363 162 833 4594
Fragments arranged by size:
4594 1363 833 162
Acc65I G'GTAC_C
Cuts at: 4273 4273
Size: 6952
AclI AA'CG_TT
Cuts at: 1563 5079 5452 1563
Size: 3516 373 3063
Fragments arranged by size:
3516 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 2278 2444 3542 3686 4044 5015 6399
Size: 620 26 166 1098 144 358 971 1384
Cuts at: 6399 6667 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1098 971 620 358 268 166
144 26
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6755
AflIII A'CryG_T
Cuts at: 1203 5851 6828 1203
Size: 4648 977 1327
Fragments arranged by size:
4648 1327 977
AgeI A'CCGG_T
Cuts at: 5783 5783
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 2083 5681 2083
Size: 3598 3354
AleI CACnn'nnGTG
Cuts at: 1166 3023 1166
Size: 1857 5095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2720 2723 3772 1184
Size: 1536 3 1049 4364
Fragments arranged by size:
4364 1536 1049 3
AlwNI CAG_nnn'CTG
Cuts at: 2284 2668 2964 3721 3867 4198 4695 6267 2284
Size: 384 296 757 146 331 497 1572 2969
Fragments arranged by size:
2969 1572 757 497 384 331 296 146
ApaI G_GGCC'C
Cuts at: 3275 3275
Size: 6952
ApaLI G'TGCA_C
Cuts at: 3230 5009 6165 3230
Size: 1779 1156 4017
Fragments arranged by size:
4017 1779 1156
ApoI r'AATT_y
Cuts at: 1493 2104 2437 2588 4261 4487 4639 6748 1493
Size: 611 333 151 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 611 333 226 152 151
AseI AT'TA_AT
Cuts at: 160 1032 5506 160
Size: 872 4474 1606
Fragments arranged by size:
4474 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
AvaI C'yCGr_G
Cuts at: 1870 1943 2246 2556 3400 3409 3828 4277 1870
Size: 73 303 310 844 9 419 449 4545
Fragments arranged by size:
4545 844 449 419 310 303 73 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2516 2549 2516
Size: 33 6919
BamHI G'GATC_C
Cuts at: 4282 4649 4282
Size: 367 6585
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2845 3053 3249 4273
Size: 325 217 627 244 814 208 196 1024
Cuts at: 4273 5728 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1024 814 627 325 244 217
208 196
BanII G_rGCy'C
Cuts at: 1391 2174 2771 3275 3410 3494 4271 1391
Size: 783 597 504 135 84 777 4072
Fragments arranged by size:
4072 783 777 597 504 135 84
BbeI G_GCGC'C
Cuts at: 2035 2035
Size: 6952
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3933 4101 928
Size: 971 2034 168 3779
Fragments arranged by size:
3779 2034 971 168
BbvCI CC'TCA_GC
Cuts at: 2577 2954 2577
Size: 377 6575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3357 3391 5143 5177 1903
Size: 34 1420 34 1752 34 3678
Fragments arranged by size:
3678 1752 1420 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4053 4844 6054 4053
Size: 791 1210 4951
Fragments arranged by size:
4951 1210 791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6952
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5563 136
Size: 107 122 71 631 605 3891 1525
Fragments arranged by size:
3891 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6947 6947
Size: 6952
BlpI GC'TnA_GC
Cuts at: 4369 4369
Size: 6952
Bme1580I G_kGCm'C
Cuts at: 1790 2850 3058 3234 3275 3388 5013 6169 1790
Size: 1060 208 176 41 113 1625 1156 2573
Fragments arranged by size:
2573 1625 1156 1060 208 176 113 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6952
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2925 4683 5641
Size: 416 53 83 399 24 1508 1758 958
Cuts at: 5641 442
Size: 1753
Fragments arranged by size:
1758 1753 1508 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6952
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 4063 3061
Size: 1002 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 2645 3128 3272 3464 3617 3920 4244
Size: 1136 270 483 144 192 153 303 324
Cuts at: 4244 5612 1239
Size: 1368 2579
Fragments arranged by size:
2579 1368 1136 483 324 303 270 192
153 144
Bpu10I CC'TnA_GC
Cuts at: 2577 2954 3260 4073 2577
Size: 377 306 813 5456
Fragments arranged by size:
5456 813 377 306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2873 2987 5077 5942 6240 2873
Size: 114 2090 865 298 3585
Fragments arranged by size:
3585 2090 865 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2235 2637 4146 5615 882
Size: 617 736 402 1509 1469 2219
Fragments arranged by size:
2219 1509 1469 736 617 402
BsaAI yAC'GTr
Cuts at: 493 1204 3021 4743 493
Size: 711 1817 1722 2702
Fragments arranged by size:
2702 1817 1722 711
BsaBI GATnn'nnATC
Cuts at: 6946 6946
Size: 6952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2032 3786 4759 5141
Size: 53 83 186 936 499 1754 973 382
Cuts at: 5141 5776 275
Size: 635 1451
Fragments arranged by size:
1754 1451 973 936 635 499 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2152 5385 5783 6057 6204 6615 2152
Size: 3233 398 274 147 411 2489
Fragments arranged by size:
3233 2489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3539 3566 3569 3596 1178
Size: 30 165 30 2136 27 3 27 4534
Fragments arranged by size:
4534 2136 165 30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 2384 2387 2936 4127 4130 4139 1186
Size: 1144 54 3 549 1191 3 9 3999
Fragments arranged by size:
3999 1191 1144 549 54 9 3 3
BseYI C'CCAG_C
Cuts at: 3275 6155 6651 3275
Size: 2880 496 3576
Fragments arranged by size:
3576 2880 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3590 3734 1163
Size: 573 1854 144 4381
Fragments arranged by size:
4381 1854 573 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2820 4402 5163 6191 1540
Size: 275 186 819 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 819 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2915 3234 3266 3410 3494 4079 5013 5098
Size: 704 319 32 144 84 585 934 85
Cuts at: 5098 6169 2211
Size: 1071 2994
Fragments arranged by size:
2994 1071 934 704 585 319 144 85
84 32
BsmI GAATG_Cn'
Cuts at: 2612 3375 4463 4556 2612
Size: 763 1088 93 5008
Fragments arranged by size:
5008 1088 763 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2795 3793 2795
Size: 998 5954
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2211 2771 2850 2915 3058 3234
Size: 399 384 37 560 79 65 143 176
Cuts at: 3234 3266 3275 3388 3410 3494 4079 4271 5013
Size: 32 9 113 22 84 585 192 742
Cuts at: 5013 5098 6169 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 585 560 399 384 192
176 143 113 85 84 79 65 37
32 22 9
BspEI T'CCGG_A
Cuts at: 2152 6615 2152
Size: 4463 2489
BspHI T'CATG_A
Cuts at: 4841 6571 4841
Size: 1730 5222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3174 3448 3549 3612 3700 4307 844
Size: 2330 274 101 63 88 607 3489
Fragments arranged by size:
3489 2330 607 274 101 88 63
BsrBI CCG'CTC
Cuts at: 1394 1823 2384 2728 3919 4839 1394
Size: 429 561 344 1191 920 3507
Fragments arranged by size:
3507 1191 920 561 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 5447 5621 65
Size: 1183 4199 174 1396
Fragments arranged by size:
4199 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2184 2967 3705 5596 5783 1700
Size: 110 374 783 738 1891 187 2869
Fragments arranged by size:
2869 1891 783 738 374 187 110
BsrGI T'GTAC_A
Cuts at: 96 3012 3213 96
Size: 2916 201 3835
Fragments arranged by size:
3835 2916 201
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6952
BssSI C'ACGA_G
Cuts at: 5012 6024 5012
Size: 1012 5940
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2618 2964 3229 3721 794
Size: 1824 346 265 492 4025
Fragments arranged by size:
4025 1824 492 346 265
BstBI TT'CG_AA
Cuts at: 5756 5756
Size: 6952
BstEII G'GTnAC_C
Cuts at: 1184 2626 1184
Size: 1442 5510
BstXI CCAn_nnnn'nTGG
Cuts at: 2782 2782
Size: 6952
BstYI r'GATC_y
Cuts at: 1972 3891 4228 4282 4649 5034 5051 6503 6589
Size: 1919 337 54 367 385 17 1452 86
Cuts at: 6589 6601 6947 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1919 1452 385 367 346 337 86
54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2460 513
Size: 552 939 456 5005
Fragments arranged by size:
5005 939 552 456
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6213
Fragments arranged by size:
6213 683 56
BtsI GCAGTG_nn'
Cuts at: 2879 3086 3232 3264 3345 3373 3619 3810 4468
Size: 207 146 32 81 28 246 191 658
Cuts at: 4468 5262 5282 2879
Size: 794 20 4549
Fragments arranged by size:
4549 794 658 246 207 191 146 81
32 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6917
DraI TTT'AAA
Cuts at: 3997 4256 3997
Size: 259 6693
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5959 809
Size: 507 4643 1802
Fragments arranged by size:
4643 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2547 2772
Size: 54 1425 50 275 18 348 369 225
Cuts at: 2772 2817 3693 4399 5288 6917 8
Size: 45 876 706 889 1629 43
Fragments arranged by size:
1629 1425 889 876 706 369 348 275
225 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2817 4399 1537
Size: 275 1005 1582 4090
Fragments arranged by size:
4090 1582 1005 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 3245 3828 4020 4098 4882 1353
Size: 963 929 583 192 78 784 3423
Fragments arranged by size:
3423 963 929 784 583 192 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 3137 5539 5913 6059 387
Size: 1064 138 465 1083 2402 374 146 1280
Fragments arranged by size:
2402 1280 1083 1064 465 374 146 138
EcoICRI GAG'CTC
Cuts at: 3408 3492 3408
Size: 84 6868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2278 2375 2444 2645 3128 3272
Size: 393 620 26 97 69 201 483 144
Cuts at: 3272 3464 3542 3617 3686 3920 4044 4244 5015
Size: 192 78 75 69 234 124 200 771
Cuts at: 5015 5612 6399 6667 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 771 620 597 483 393 268
234 201 200 192 144 124 97 78
75 69 69 26
EcoO109I rG'GnC_Cy
Cuts at: 3256 3272 3414 4068 3256
Size: 16 142 654 6140
Fragments arranged by size:
6140 654 142 16
EcoRI G'AATT_C
Cuts at: 2437 4261 4639 2437
Size: 1824 378 4750
Fragments arranged by size:
4750 1824 378
FspI TGC'GCA
Cuts at: 3170 5458 3170
Size: 2288 4664
HaeII r_GCGC'y
Cuts at: 1678 2035 6099 1678
Size: 357 4064 2531
Fragments arranged by size:
4064 2531 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2075 2101 2107 2207
Size: 32 135 32 497 6 26 6 100
Cuts at: 2207 2239 5593 5625 5667 5699 1373
Size: 32 3354 32 42 32 2626
Fragments arranged by size:
3354 2626 497 135 100 42 32 32
32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3463 3538 4296 4542 669
Size: 988 281 1525 75 758 246 3079
Fragments arranged by size:
3079 1525 988 758 281 246 75
HindIII A'AGCT_T
Cuts at: 748 2738 748
Size: 1990 4962
HpaI GTT'AAC
Cuts at: 4542 4542
Size: 6952
KasI G'GCGC_C
Cuts at: 2031 2031
Size: 6952
KpnI G_GTAC'C
Cuts at: 4277 4277
Size: 6952
MfeI C'AATT_G
Cuts at: 4551 4551
Size: 6952
MluI A'CGCG_T
Cuts at: 6828 6828
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2778 6066 6250 1305
Size: 1473 3288 184 2007
Fragments arranged by size:
3288 2007 1473 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 3695 10
Size: 54 1425 343 717 1146 3267
Fragments arranged by size:
3267 1425 1146 717 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 3023 3425 4910 5269 5428 518
Size: 648 1671 186 402 1485 359 159 2042
Fragments arranged by size:
2042 1671 1485 648 402 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2543 2782 3364 3580 3664 3721 3898
Size: 406 531 239 582 216 84 57 177
Cuts at: 3898 4198 4368 5045 6193 6438 1606
Size: 300 170 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 582 531 406 300 245
239 216 177 170 84 57
NaeI GCC'GGC
Cuts at: 2186 2969 3707 2186
Size: 783 738 5431
Fragments arranged by size:
5431 783 738
NarI GG'CG_CC
Cuts at: 2032 2032
Size: 6952
NcoI C'CATG_G
Cuts at: 513 1065 2004 2460 513
Size: 552 939 456 5005
Fragments arranged by size:
5005 939 552 456
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6952
NgoMIV G'CCGG_C
Cuts at: 2184 2967 3705 2184
Size: 783 738 5431
Fragments arranged by size:
5431 783 738
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6952
NotI GC'GGCC_GC
Cuts at: 4399 4399
Size: 6952
NspI r_CATG'y
Cuts at: 1132 1229 2884 4310 5855 1132
Size: 97 1655 1426 1545 2229
Fragments arranged by size:
2229 1655 1545 1426 97
PasI CC'CwG_GG
Cuts at: 1348 4181 4204 1348
Size: 2833 23 4096
Fragments arranged by size:
4096 2833 23
PciI A'CATG_T
Cuts at: 5851 5851
Size: 6952
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2521 3295 1108
Size: 1413 774 4765
Fragments arranged by size:
4765 1413 774
PfoI T'CCnGG_A
Cuts at: 3441 3633 3678 3441
Size: 192 45 6715
Fragments arranged by size:
6715 192 45
PmeI GTTT'AAAC
Cuts at: 4256 4256
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2315 2347 5021 5053 6534 6566 2315
Size: 32 2674 32 1481 32 2701
Fragments arranged by size:
2701 2674 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3256 3256
Size: 6952
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6952
PsiI TTA'TAA
Cuts at: 4522 4522
Size: 6952
PspOMI G'GGCC_C
Cuts at: 3271 3271
Size: 6952
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 6920
PstI C_TGCA'G
Cuts at: 830 2268 2712 3546 3663 3761 4194 4236 4304
Size: 1438 444 834 117 98 433 42 68
Cuts at: 4304 830
Size: 3478
Fragments arranged by size:
3478 1438 834 444 433 117 98 68
42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6952
PvuII CAG'CTG
Cuts at: 2012 2543 2782 3580 3664 3721 3898 4198 2012
Size: 531 239 798 84 57 177 300 4766
Fragments arranged by size:
4766 798 531 300 239 177 84 57
SacI G_AGCT'C
Cuts at: 3410 3494 3410
Size: 84 6868
SalI G'TCGA_C
Cuts at: 1936 3461 4294 1936
Size: 1525 833 4594
Fragments arranged by size:
4594 1525 833
SbfI CC_TGCA'GG
Cuts at: 2268 3546 4304 2268
Size: 1278 758 4916
Fragments arranged by size:
4916 1278 758
ScaI AGT'ACT
Cuts at: 5200 6912 5200
Size: 1712 5240
SfcI C'TryA_G
Cuts at: 826 1045 2264 2708 3542 3659 3757 3859 4190
Size: 219 1219 444 834 117 98 102 331
Cuts at: 4190 4232 4300 5435 6116 6307 826
Size: 42 68 1135 681 191 1471
Fragments arranged by size:
1471 1219 1135 834 681 444 331 219
191 117 102 98 68 42
SfoI GGC'GCC
Cuts at: 2033 2033
Size: 6952
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6952
SmaI CCC'GGG
Cuts at: 1872 3830 4279 1872
Size: 1958 449 4545
Fragments arranged by size:
4545 1958 449
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2888 3002 5056 5957 6219 820
Size: 197 926 945 114 2054 901 262 1553
Fragments arranged by size:
2054 1553 945 926 901 262 197 114
SnaBI TAC'GTA
Cuts at: 493 4743 493
Size: 4250 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6952
SphI G_CATG'C
Cuts at: 1132 2884 4310 1132
Size: 1752 1426 3774
Fragments arranged by size:
3774 1752 1426
SspI AAT'ATT
Cuts at: 5 52 4876 5
Size: 47 4824 2081
Fragments arranged by size:
4824 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6952
StyI C'CwwG_G
Cuts at: 513 1065 2004 2460 2989 3294 4391 513
Size: 552 939 456 529 305 1097 3074
Fragments arranged by size:
3074 1097 939 552 529 456 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4979 4996 5149 5334 5779 325
Size: 1255 3399 17 153 185 445 1498
Fragments arranged by size:
3399 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3012 3213 3855
Size: 274 80 33 51 1443 1035 201 642
Cuts at: 3855 5198 6910 96
Size: 1343 1712 138
Fragments arranged by size:
1712 1443 1343 1035 642 274 201 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2728 2760 6912 6944 2728
Size: 32 4152 32 2736
Fragments arranged by size:
4152 2736 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2408 1316
Size: 1092 5860
XbaI T'CTAG_A
Cuts at: 4288 4288
Size: 6952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3320 3320
Size: 6952
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6952
XmaI C'CCGG_G
Cuts at: 1870 3828 4277 1870
Size: 1958 449 4545
Fragments arranged by size:
4545 1958 449
XmnI GAAnn'nnTTC
Cuts at: 3671 5081 3671
Size: 1410 5542
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4760 276
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI