Restriction map for
pFN21AB8567
(Circular) (Six-base) MAPSORT of: pFN21AB8567.seq Check: 9938 from: 1 to: 5899
pFN21AB8567 5899 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3146 3962 5346 5614 1632
Size: 1514 816 1384 268 1917
Fragments arranged by size:
1917 1514 1384 816 268
AflII C'TTAA_G
Cuts at: 820 1017 2812 820
Size: 197 1795 3907
Fragments arranged by size:
3907 1795 197
AflIII A'CryG_T
Cuts at: 1203 2568 4798 5775 1203
Size: 1365 2230 977 1327
Fragments arranged by size:
2230 1365 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 2936 3642 5214 2936
Size: 706 1572 3621
Fragments arranged by size:
3621 1572 706
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 2777 3083 3208 3434 3586 5695 1493
Size: 1284 306 125 226 152 2109 1697
Fragments arranged by size:
2109 1697 1284 306 226 152 125
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 3224 1870
Size: 73 1281 4545
Fragments arranged by size:
4545 1281 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3220 4675 618
Size: 325 217 627 1433 1455 1842
Fragments arranged by size:
1842 1455 1433 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3218 1391
Size: 1827 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2703 928
Size: 971 804 4124
Fragments arranged by size:
4124 971 804
BbvCI CC'TCA_GC
Cuts at: 2388 3144 2388
Size: 756 5143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2999 3791 5001 2999
Size: 792 1210 3897
Fragments arranged by size:
3897 1210 792
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4510 136
Size: 107 122 71 631 605 2838 1525
Fragments arranged by size:
2838 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 3960 5116 1790
Size: 2170 1156 2573
Fragments arranged by size:
2573 2170 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5899
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3630 4588 442
Size: 416 53 83 399 24 2213 958 1753
Fragments arranged by size:
2213 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 4559 1239
Size: 1220 2100 2579
Fragments arranged by size:
2579 2100 1220
Bpu10I CC'TnA_GC
Cuts at: 2388 3144 2388
Size: 756 5143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4024 4889 5187 4024
Size: 865 298 4736
Fragments arranged by size:
4736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4562 882
Size: 617 3063 2219
Fragments arranged by size:
3063 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3690 493
Size: 711 2486 2702
Fragments arranged by size:
2702 2486 711
BsaBI GATnn'nnATC
Cuts at: 2418 5893 2418
Size: 3475 2424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 3706 4088 4723
Size: 53 83 186 936 487 1686 382 635
Cuts at: 4723 275
Size: 1451
Fragments arranged by size:
1686 1451 936 635 487 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 3161 3191 1178
Size: 30 165 30 1146 30 582 30 3886
Fragments arranged by size:
3886 1146 582 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2687 1186
Size: 1501 4398
BseYI C'CCAG_C
Cuts at: 5102 5598 5102
Size: 496 5403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5326
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3349 4110 5138 1540
Size: 275 186 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3960 4045 5116 3960
Size: 85 1071 4743
Fragments arranged by size:
4743 1071 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3218 3960 4045 5116 1391
Size: 399 1428 742 85 1071 2174
Fragments arranged by size:
2174 1428 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3254 844
Size: 2410 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3786 1394
Size: 429 1963 3507
Fragments arranged by size:
3507 1963 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4394 4568 65
Size: 1183 3146 174 1396
Fragments arranged by size:
3146 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4543 4730 1700
Size: 110 2733 187 2869
Fragments arranged by size:
2869 2733 187 110
BsrGI T'GTAC_A
Cuts at: 96 2565 96
Size: 2469 3430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5899
BssSI C'ACGA_G
Cuts at: 3959 4971 3959
Size: 1012 4887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2936 794
Size: 2142 3757
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2512 3055 2041
Size: 471 543 4885
Fragments arranged by size:
4885 543 471
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2289 2289
Size: 5899
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4408
Fragments arranged by size:
4408 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3073 487
Size: 683 56 1847 3313
Fragments arranged by size:
3313 1847 683 56
BtsI GCAGTG_nn'
Cuts at: 2404 3415 4209 4229 2404
Size: 1011 794 20 4074
Fragments arranged by size:
4074 1011 794 20
ClaI AT'CG_AT
Cuts at: 1554 2590 1554
Size: 1036 4863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3045 3346 4235
Size: 54 1425 50 275 18 1215 301 889
Cuts at: 4235 5864 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1215 889 301 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3346 1537
Size: 275 1534 4090
Fragments arranged by size:
4090 1534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2304 2473 3829 1353
Size: 951 169 1356 3423
Fragments arranged by size:
3423 1356 951 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4486 4860 5006 387
Size: 1064 138 2897 374 146 1280
Fragments arranged by size:
2897 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2459 3146 3962 4559 5346 5614 1239
Size: 393 827 687 816 597 787 268 1524
Fragments arranged by size:
1524 827 816 787 687 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2273 2273
Size: 5899
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
EcoRV GAT'ATC
Cuts at: 2416 2416
Size: 5899
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 2012 5046 1678
Size: 334 3034 2531
Fragments arranged by size:
3034 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2632 2664 2668 2700 3161
Size: 32 135 32 1060 32 4 32 461
Cuts at: 3161 3193 4540 4572 4614 4646 1373
Size: 32 1347 32 42 32 2626
Fragments arranged by size:
2626 1347 1060 461 135 42 32 32
32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 3243 3489 669
Size: 988 281 271 1034 246 3079
Fragments arranged by size:
3079 1034 988 281 271 246
HindIII A'AGCT_T
Cuts at: 748 2325 2624 748
Size: 1577 299 4023
Fragments arranged by size:
4023 1577 299
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2041 5013 5197 1305
Size: 736 2972 184 2007
Fragments arranged by size:
2972 2007 736 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3047 10
Size: 54 1425 343 1215 2862
Fragments arranged by size:
2862 1425 1215 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2510 3857 4216 4375 518
Size: 648 1344 1347 359 159 2042
Fragments arranged by size:
2042 1347 1344 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2437 2936 3148 3315 3992 5140 5385 1606
Size: 831 499 212 167 677 1148 245 2120
Fragments arranged by size:
2120 1148 831 677 499 245 212 167
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4408
Fragments arranged by size:
4408 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 2500 2572 3257 4802 1132
Size: 97 1271 72 685 1545 2229
Fragments arranged by size:
2229 1545 1271 685 97 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 2568 4798 2568
Size: 2230 3669
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5899
PfoI T'CCnGG_A
Cuts at: 2615 2615
Size: 5899
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2273 2273
Size: 5899
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PstI C_TGCA'G
Cuts at: 830 2399 2556 3251 830
Size: 1569 157 695 3478
Fragments arranged by size:
3478 1569 695 157
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
PvuII CAG'CTG
Cuts at: 2437 2936 3148 2437
Size: 499 212 5188
Fragments arranged by size:
5188 499 212
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2395 2552 3247 4382 5063 5254 826
Size: 219 1350 157 695 1135 681 191 1471
Fragments arranged by size:
1471 1350 1135 695 681 219 191 157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 3226 1872
Size: 1354 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2812 4003 4904 5166 820
Size: 197 926 869 1191 901 262 1553
Fragments arranged by size:
1553 1191 926 901 869 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 2500 3257 1132
Size: 1368 757 3774
Fragments arranged by size:
3774 1368 757
SspI AAT'ATT
Cuts at: 5 52 2050 2692 3823 5
Size: 47 1998 642 1131 2081
Fragments arranged by size:
2081 1998 1131 642 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 2004 3338 513
Size: 552 939 1334 3074
Fragments arranged by size:
3074 1334 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3926 3943 4096 4281 4726 325
Size: 1255 2346 17 153 185 445 1498
Fragments arranged by size:
2346 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2245 2565 4145
Size: 274 80 33 51 1443 268 320 1580
Cuts at: 4145 5857 96
Size: 1712 138
Fragments arranged by size:
1712 1580 1443 320 274 268 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2161 2193 5859 5891 2161
Size: 32 3666 32 2169
Fragments arranged by size:
3666 2169 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2029 1316
Size: 713 5186
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2872 2872
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 3224 1870
Size: 1354 4545
XmnI GAAnn'nnTTC
Cuts at: 4028 4028
Size: 5899
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI