Restriction map for
pFN21AB8564
(Circular) (Six-base) MAPSORT of: pFN21AB8564.seq Check: 1865 from: 1 to: 7438
pFN21AB8564 7438 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4433 4433
Size: 7438
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2364 4672 5248 278
Size: 53 83 186 936 828 2308 576 2468
Fragments arranged by size:
2468 2308 936 828 576 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3054 4781 1937
Size: 1117 1727 4594
Fragments arranged by size:
4594 1727 1117
Acc65I G'GTAC_C
Cuts at: 3322 3812 4218 4759 3322
Size: 490 406 541 6001
Fragments arranged by size:
6001 541 490 406
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2781 3858 5501 6885 7153 1632
Size: 1149 1077 1643 1384 268 1917
Fragments arranged by size:
1917 1643 1384 1149 1077 268
AfeI AGC'GCT
Cuts at: 2966 2966
Size: 7438
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 6337 7314 1203
Size: 5134 977 1327
Fragments arranged by size:
5134 1327 977
AgeI A'CCGG_T
Cuts at: 2316 4215 4530 6269 2316
Size: 1899 315 1739 3485
Fragments arranged by size:
3485 1899 1739 315
AhdI GACnn_n'nnGTC
Cuts at: 6167 6167
Size: 7438
AleI CACnn'nnGTG
Cuts at: 1166 3975 1166
Size: 2809 4629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2598 2631 3573 1184
Size: 1414 33 942 5049
Fragments arranged by size:
5049 1414 942 33
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 7406
AlwNI CAG_nnn'CTG
Cuts at: 3125 3966 5181 6753 3125
Size: 841 1215 1572 3810
Fragments arranged by size:
3810 1572 1215 841
ApaI G_GGCC'C
Cuts at: 2530 3476 3805 2530
Size: 946 329 6163
Fragments arranged by size:
6163 946 329
ApaLI G'TGCA_C
Cuts at: 5495 6651 5495
Size: 1156 6282
ApoI r'AATT_y
Cuts at: 1493 3302 4747 4973 5125 7234 1493
Size: 1809 1445 226 152 2109 1697
Fragments arranged by size:
2109 1809 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 2253 2699 3469 3738 4190 4635 4763
Size: 73 310 446 770 269 452 445 128
Cuts at: 4763 1870
Size: 4545
Fragments arranged by size:
4545 770 452 446 445 310 269 128
73
AvrII C'CTAG_G
Cuts at: 3287 3287
Size: 7438
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2386 2419 2386
Size: 33 7405
BamHI G'GATC_C
Cuts at: 4768 5135 4768
Size: 367 7071
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 3322 3529 3773 3812
Size: 325 217 627 369 1166 207 244 39
Cuts at: 3812 4094 4218 4533 4759 6214 618
Size: 282 124 315 226 1455 1842
Fragments arranged by size:
1842 1455 1166 627 369 325 315 282
244 226 217 207 124 39
BanII G_rGCy'C
Cuts at: 1391 2225 2498 2530 2557 3462 3476 3805 4642
Size: 834 273 32 27 905 14 329 837
Cuts at: 4642 4757 1391
Size: 115 4072
Fragments arranged by size:
4072 905 837 834 329 273 115 32
27 14
BbeI G_GCGC'C
Cuts at: 2160 3533 4098 2160
Size: 1373 565 5500
Fragments arranged by size:
5500 1373 565
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2769 3276 4633 928
Size: 971 870 507 1357 3733
Fragments arranged by size:
3733 1357 971 870 507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5629 5663 1903
Size: 34 3692 34 3678
Fragments arranged by size:
3692 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2658 5330 6540 2658
Size: 2672 1210 3556
Fragments arranged by size:
3556 2672 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7438
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2534 3800 6049
Size: 107 122 71 631 605 862 1266 2249
Cuts at: 6049 136
Size: 1525
Fragments arranged by size:
2249 1525 1266 862 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4608 7433 4608
Size: 2825 4613
BlpI GC'TnA_GC
Cuts at: 2375 3461 4855 2375
Size: 1086 1394 4958
Fragments arranged by size:
4958 1394 1086
Bme1580I G_kGCm'C
Cuts at: 1790 2530 2545 3476 3778 3805 4401 4623 5499
Size: 740 15 931 302 27 596 222 876
Cuts at: 5499 6655 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 931 876 740 596 302 222
27 15
BmgBI CAC'GTC
Cuts at: 1318 2179 4396 1318
Size: 861 2217 4360
Fragments arranged by size:
4360 2217 861
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4141 4694 5169
Size: 416 53 83 399 24 2724 553 475
Cuts at: 5169 6127 442
Size: 958 1753
Fragments arranged by size:
2724 1753 958 553 475 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2924 1056
Size: 1868 5570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3850 3850
Size: 7438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2781 3185 3249 3845 4365 6098 1239
Size: 1542 404 64 596 520 1733 2579
Fragments arranged by size:
2579 1733 1542 596 520 404 64
Bpu10I CC'TnA_GC
Cuts at: 3720 3720
Size: 7438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4461 5563 6428 6726 4461
Size: 1102 865 298 5173
Fragments arranged by size:
5173 1102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3152 4136 6101 882
Size: 617 1653 984 1965 2219
Fragments arranged by size:
2219 1965 1653 984 617
BsaAI yAC'GTr
Cuts at: 493 1204 5229 493
Size: 711 4025 2702
Fragments arranged by size:
4025 2702 711
BsaBI GATnn'nnATC
Cuts at: 7432 7432
Size: 7438
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 2361 3530 4083
Size: 53 83 186 936 624 204 1169 553
Cuts at: 4083 4095 4287 4669 5245 5627 6262 275
Size: 12 192 382 576 382 635 1451
Fragments arranged by size:
1451 1169 936 635 624 576 553 382
382 204 192 186 83 53 12
BsaWI w'CCGG_w
Cuts at: 2316 2706 4215 4459 4530 5871 6269 6543 6690
Size: 390 1509 244 71 1341 398 274 147
Cuts at: 6690 7101 2316
Size: 411 2653
Fragments arranged by size:
2653 1509 1341 411 398 390 274 244
147 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2280 2310 3095 3125 3915
Size: 30 165 30 877 30 785 30 790
Cuts at: 3915 3945 4217 4247 1178
Size: 30 272 30 4369
Fragments arranged by size:
4369 877 790 785 272 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3261 3416 3431 3434 3756 3767 4007 4010
Size: 2075 155 15 3 322 11 240 3
Cuts at: 4010 4148 4667 1186
Size: 138 519 3957
Fragments arranged by size:
3957 2075 519 322 240 155 138 15
11 3 3
BseYI C'CCAG_C
Cuts at: 2010 2218 2344 2957 3075 3106 3118 3274 3452
Size: 208 126 613 118 31 12 156 178
Cuts at: 3452 3862 3885 3959 4077 4128 4551 6641 7137
Size: 410 23 74 118 51 423 2090 496
Cuts at: 7137 2010
Size: 2311
Fragments arranged by size:
2311 2090 613 496 423 410 208 178
156 126 118 118 74 51 31 23
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2868 3899 4628 1163
Size: 573 1132 1031 729 3973
Fragments arranged by size:
3973 1132 1031 729 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4489 4591 4888 5649 6677 1540
Size: 275 186 2488 102 297 761 1028 2301
Fragments arranged by size:
2488 2301 1028 761 297 275 186 102
BsiHKAI G_wGCw'C
Cuts at: 2151 3462 4642 5499 5584 6655 2151
Size: 1311 1180 857 85 1071 2934
Fragments arranged by size:
2934 1311 1180 1071 857 85
BsmI GAATG_Cn'
Cuts at: 4949 5042 4949
Size: 93 7345
BsmBI CGTCTCn'nnnn_
Cuts at: 2368 2368
Size: 7438
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2225 2498 2530 2545 2557 3462
Size: 399 361 74 273 32 15 12 905
Cuts at: 3462 3476 3778 3805 4401 4623 4642 4757 5499
Size: 14 302 27 596 222 19 115 742
Cuts at: 5499 5584 6655 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 905 742 596 399 361 302
273 222 115 85 74 32 27 19
15 14 12
BspEI T'CCGG_A
Cuts at: 2706 7101 2706
Size: 4395 3043
BspHI T'CATG_A
Cuts at: 5327 7057 5327
Size: 1730 5708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 4433 4639 4793 844
Size: 1352 2237 206 154 3489
Fragments arranged by size:
3489 2237 1352 206 154
BsrBI CCG'CTC
Cuts at: 1394 1823 2842 5325 1394
Size: 429 1019 2483 3507
Fragments arranged by size:
3507 2483 1019 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2615 5933 6107 65
Size: 1183 1367 3318 174 1396
Fragments arranged by size:
3318 1396 1367 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2316 4215 4530 6082 6269 1700
Size: 110 506 1899 315 1552 187 2869
Fragments arranged by size:
2869 1899 1552 506 315 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7438
BssHII G'CGCG_C
Cuts at: 1925 4164 1925
Size: 2239 5199
BssSI C'ACGA_G
Cuts at: 2664 5498 6510 2664
Size: 2834 1012 3592
Fragments arranged by size:
3592 2834 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2146 2336 2855 794
Size: 1352 190 519 5377
Fragments arranged by size:
5377 1352 519 190
BstBI TT'CG_AA
Cuts at: 6242 6242
Size: 7438
BstEII G'GTnAC_C
Cuts at: 1184 2789 1184
Size: 1605 5833
BstXI CCAn_nnnn'nTGG
Cuts at: 2969 3823 3977 2969
Size: 854 154 6430
Fragments arranged by size:
6430 854 154
BstYI r'GATC_y
Cuts at: 1972 4608 4768 5135 5520 5537 6989 7075 7087
Size: 2636 160 367 385 17 1452 86 12
Cuts at: 7087 7433 1972
Size: 346 1977
Fragments arranged by size:
2636 1977 1452 385 367 346 160 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3093 3183 3477 3807 3093
Size: 90 294 330 6724
Fragments arranged by size:
6724 330 294 90
BtgI C'CryG_G
Cuts at: 513 1065 2004 2228 2862 3208 3318 3330 3610
Size: 552 939 224 634 346 110 12 280
Cuts at: 3610 3930 4493 513
Size: 320 563 3458
Fragments arranged by size:
3458 939 634 563 552 346 320 280
224 110 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2104 2545 4062 487
Size: 683 56 878 441 1517 3863
Fragments arranged by size:
3863 1517 878 683 441 56
BtsI GCAGTG_nn'
Cuts at: 2135 2330 2902 2938 4954 5748 5768 2135
Size: 195 572 36 2016 794 20 3805
Fragments arranged by size:
3805 2016 794 572 195 36 20
ClaI AT'CG_AT
Cuts at: 1554 2433 1554
Size: 879 6559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3664 3678 3699 3713 529
Size: 35 3100 14 21 14 4254
Fragments arranged by size:
4254 3100 35 21 14 14
DraI TTT'AAA
Cuts at: 4742 4742
Size: 7438
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2248 6445 809
Size: 507 932 4197 1802
Fragments arranged by size:
4197 1802 932 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2192 3927 4173
Size: 54 1425 50 275 18 362 1735 246
Cuts at: 4173 4486 4885 5774 7403 8
Size: 313 399 889 1629 43
Fragments arranged by size:
1735 1629 1425 889 399 362 313 275
246 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4486 4885 1537
Size: 275 2674 399 4090
Fragments arranged by size:
4090 2674 399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3005 3011 3339 3366 3387 3408 5368 1353
Size: 1652 6 328 27 21 21 1960 3423
Fragments arranged by size:
3423 1960 1652 328 27 21 21 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6025 6399 6545 387
Size: 1064 138 4436 374 146 1280
Fragments arranged by size:
4436 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3460 4640 3460
Size: 1180 6258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2781 3185 3249 3845 3858 4365 5501
Size: 393 1149 404 64 596 13 507 1136
Cuts at: 5501 6098 6885 7153 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1149 1136 787 597 596 507 404
393 268 64 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2048
Size: 7438
EcoO109I rG'GnC_Cy
Cuts at: 2526 3283 3473 3733 3802 3829 3870 4039 4600
Size: 757 190 260 69 27 41 169 561
Cuts at: 4600 4657 4733 2526
Size: 57 76 5231
Fragments arranged by size:
5231 757 561 260 190 169 76 69
57 41 27
EcoRI G'AATT_C
Cuts at: 4747 5125 4747
Size: 378 7060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 7438
FspI TGC'GCA
Cuts at: 2117 5944 2117
Size: 3827 3611
FspAI rTGC'GCAy
Cuts at: 2117 2117
Size: 7438
HaeII r_GCGC'y
Cuts at: 1678 2160 2375 2968 3533 3912 4098 4703 6585
Size: 482 215 593 565 379 186 605 1882
Cuts at: 6585 1678
Size: 2531
Fragments arranged by size:
2531 1882 605 593 565 482 379 215
186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2280 2312 2544 2576 3188
Size: 32 135 32 708 32 232 32 612
Cuts at: 3188 3220 3566 3598 6079 6111 6153 6185 1373
Size: 32 346 32 2481 32 42 32 2626
Fragments arranged by size:
2626 2481 708 612 346 232 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4782 5028 669
Size: 988 281 2844 246 3079
Fragments arranged by size:
3079 2844 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3588 748
Size: 2840 4598
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KasI G'GCGC_C
Cuts at: 2156 3529 4094 2156
Size: 1373 565 5500
Fragments arranged by size:
5500 1373 565
KpnI G_GTAC'C
Cuts at: 3326 3816 4222 4763 3326
Size: 490 406 541 6001
Fragments arranged by size:
6001 541 490 406
MfeI C'AATT_G
Cuts at: 5037 5037
Size: 7438
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2610 3126 3138 4260 6552 6736 1305
Size: 1305 516 12 1122 2292 184 2007
Fragments arranged by size:
2292 2007 1305 1122 516 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4175 10
Size: 54 1425 343 2343 3273
Fragments arranged by size:
3273 2343 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3975 5396 5755 5914 518
Size: 648 2809 1421 359 159 2042
Fragments arranged by size:
2809 2042 1421 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2230 3178 4011 4567 4854 5531 6679
Size: 408 216 948 833 556 287 677 1148
Cuts at: 6679 6924 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 948 833 677 556 408 287
245 216
NarI GG'CG_CC
Cuts at: 2157 3530 4095 2157
Size: 1373 565 5500
Fragments arranged by size:
5500 1373 565
NcoI C'CATG_G
Cuts at: 513 1065 2004 3318 3610 513
Size: 552 939 1314 292 4341
Fragments arranged by size:
4341 1314 939 552 292
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7438
NheI G'CTAG_C
Cuts at: 1052 2920 1052
Size: 1868 5570
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 3987 4796 6341 1132
Size: 97 2758 809 1545 2229
Fragments arranged by size:
2758 2229 1545 809 97
PasI CC'CwG_GG
Cuts at: 1348 2912 3642 4316 1348
Size: 1564 730 674 4470
Fragments arranged by size:
4470 1564 730 674
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7438
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5507 5539 7020 7052 5507
Size: 32 1481 32 5893
Fragments arranged by size:
5893 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3733 3870 4039 4657 4733 3733
Size: 137 169 618 76 6438
Fragments arranged by size:
6438 618 169 137 76
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 5008 5008
Size: 7438
PspOMI G'GGCC_C
Cuts at: 2526 3472 3801 2526
Size: 946 329 6163
Fragments arranged by size:
6163 946 329
PspXI vC'TCGA_Gb
Cuts at: 3738 3738
Size: 7438
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2297 2329 2297
Size: 32 7406
PstI C_TGCA'G
Cuts at: 830 2062 2142 2909 3177 3552 4790 830
Size: 1232 80 767 268 375 1238 3478
Fragments arranged by size:
3478 1238 1232 767 375 268 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 2014 3178 2014
Size: 1164 6274
SacI G_AGCT'C
Cuts at: 3462 4642 3462
Size: 1180 6258
SacII CC_GC'GG
Cuts at: 2231 2231
Size: 7438
SalI G'TCGA_C
Cuts at: 1936 4780 1936
Size: 2844 4594
SanDI GG'GwC_CC
Cuts at: 3733 3733
Size: 7438
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 5686 7398 5686
Size: 1712 5726
SfcI C'TryA_G
Cuts at: 826 1045 2058 2138 2905 3173 3548 4786 5921
Size: 219 1013 80 767 268 375 1238 1135
Cuts at: 5921 6602 6793 826
Size: 681 191 1471
Fragments arranged by size:
1471 1238 1135 1013 767 681 375 268
219 191 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 2534 2534
Size: 7438
SfoI GGC'GCC
Cuts at: 2158 3531 4096 2158
Size: 1373 565 5500
Fragments arranged by size:
5500 1373 565
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 2255 2701 3471 4765 1872
Size: 383 446 770 1294 4545
Fragments arranged by size:
4545 1294 770 446 383
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3738 4476 5542 6443 6705 820
Size: 197 926 1795 738 1066 901 262 1553
Fragments arranged by size:
1795 1553 1066 926 901 738 262 197
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4796 1132
Size: 3664 3774
SrfI GCCC'GGGC
Cuts at: 3471 3471
Size: 7438
SspI AAT'ATT
Cuts at: 5 52 5362 5
Size: 47 5310 2081
Fragments arranged by size:
5310 2081 47
StuI AGG'CCT
Cuts at: 1517 2312 2537 2672 3098 3482 3598 1517
Size: 795 225 135 426 384 116 5357
Fragments arranged by size:
5357 795 426 384 225 135 116
StyI C'CwwG_G
Cuts at: 513 1065 2004 2593 3287 3318 3602 3610 4877
Size: 552 939 589 694 31 284 8 1267
Cuts at: 4877 513
Size: 3074
Fragments arranged by size:
3074 1267 939 694 589 552 284 31
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2777 2971 4662 5465 5482 5635 5820
Size: 1255 1197 194 1691 803 17 153 185
Cuts at: 5820 6265 325
Size: 445 1498
Fragments arranged by size:
1691 1498 1255 1197 803 445 194 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2748 5684 7396
Size: 274 80 33 51 1443 771 2936 1712
Cuts at: 7396 96
Size: 138
Fragments arranged by size:
2936 1712 1443 771 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 3915 3947 4217 4249 7398 7430 2701
Size: 32 1182 32 270 32 3149 32 2709
Fragments arranged by size:
3149 2709 1182 270 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3953 1316
Size: 2637 4801
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2969 3232 2969
Size: 263 7175
XhoI C'TCGA_G
Cuts at: 1943 3738 1943
Size: 1795 5643
XmaI C'CCGG_G
Cuts at: 1870 2253 2699 3469 4763 1870
Size: 383 446 770 1294 4545
Fragments arranged by size:
4545 1294 770 446 383
XmnI GAAnn'nnTTC
Cuts at: 5567 5567
Size: 7438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2362 4670 5246 276
Size: 53 83 186 936 828 2308 576 2468
Fragments arranged by size:
2468 2308 936 828 576 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FseI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI RsrII
SapI SexAI SwaI