Restriction map for
pFN21AB8562
(Circular) (Six-base) MAPSORT of: pFN21AB8562.seq Check: 4686 from: 1 to: 7438
pFN21AB8562 7438 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5248 278
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4781 1937
Size: 2844 4594
Acc65I G'GTAC_C
Cuts at: 2162 3743 4759 2162
Size: 1581 1016 4841
Fragments arranged by size:
4841 1581 1016
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4621 4637 5501 6885 7153 1632
Size: 2989 16 864 1384 268 1917
Fragments arranged by size:
2989 1917 1384 864 268 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 3672 6337 7314 1203
Size: 2469 2665 977 1327
Fragments arranged by size:
2665 2469 1327 977
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 3450 4298 6167 3450
Size: 848 1869 4721
Fragments arranged by size:
4721 1869 848
AleI CACnn'nnGTG
Cuts at: 1166 2687 3338 4187 1166
Size: 1521 651 849 4417
Fragments arranged by size:
4417 1521 849 651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2978 1184
Size: 1794 5644
AlwNI CAG_nnn'CTG
Cuts at: 2194 2221 2566 2998 3220 3379 4250 4660 5181
Size: 27 345 432 222 159 871 410 521
Cuts at: 5181 6753 2194
Size: 1572 2879
Fragments arranged by size:
2879 1572 871 521 432 410 345 222
159 27
ApaI G_GGCC'C
Cuts at: 2473 3107 3701 2473
Size: 634 594 6210
Fragments arranged by size:
6210 634 594
ApaLI G'TGCA_C
Cuts at: 5495 6651 5495
Size: 1156 6282
ApoI r'AATT_y
Cuts at: 1493 2649 4747 4973 5125 7234 1493
Size: 1156 2098 226 152 2109 1697
Fragments arranged by size:
2109 2098 1697 1156 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 2158 3939 4763 1870
Size: 73 141 74 1781 824 4545
Fragments arranged by size:
4545 1781 824 141 74 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2146 2179 4439 4472 2146
Size: 33 2260 33 5112
Fragments arranged by size:
5112 2260 33 33
BamHI G'GATC_C
Cuts at: 2551 4768 5135 2551
Size: 2217 367 4854
Fragments arranged by size:
4854 2217 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2782 3010 3165 3682
Size: 325 217 627 375 620 228 155 517
Cuts at: 3682 3743 3904 4759 6214 618
Size: 61 161 855 1455 1842
Fragments arranged by size:
1842 1455 855 627 620 517 375 325
228 217 161 155 61
BanII G_rGCy'C
Cuts at: 1391 2473 3107 3373 3701 4103 4338 4757 1391
Size: 1082 634 266 328 402 235 419 4072
Fragments arranged by size:
4072 1082 634 419 402 328 266 235
BbeI G_GCGC'C
Cuts at: 3169 3169
Size: 7438
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 2076 2335 2436 3662 4018 4496
Size: 971 138 39 259 101 1226 356 478
Cuts at: 4496 928
Size: 3870
Fragments arranged by size:
3870 1226 971 478 356 259 138 101
39
BbvCI CC'TCA_GC
Cuts at: 3934 3934
Size: 7438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3337 3371 5629 5663 1903
Size: 34 1400 34 2258 34 3678
Fragments arranged by size:
3678 2258 1400 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3874 5330 6540 3874
Size: 1456 1210 4772
Fragments arranged by size:
4772 1456 1210
BclI T'GATC_A
Cuts at: 1548 3624 3849 1548
Size: 2076 225 5137
Fragments arranged by size:
5137 2076 225
BfrBI ATG'CAT
Cuts at: 3203 3203
Size: 7438
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2903 4574 4612
Size: 107 122 71 631 605 1231 1671 38
Cuts at: 4612 6049 136
Size: 1437 1525
Fragments arranged by size:
1671 1525 1437 1231 631 605 122 107
71 38
BglII A'GATC_T
Cuts at: 3570 3630 7433 3570
Size: 60 3803 3575
Fragments arranged by size:
3803 3575 60
BlpI GC'TnA_GC
Cuts at: 3372 4403 4855 3372
Size: 1031 452 5955
Fragments arranged by size:
5955 1031 452
Bme1580I G_kGCm'C
Cuts at: 1790 2473 2627 2637 3107 3687 3701 4473 5499
Size: 683 154 10 470 580 14 772 1026
Cuts at: 5499 6655 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1026 772 683 580 470 154
14 10
BmgBI CAC'GTC
Cuts at: 1318 4398 1318
Size: 3080 4358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3971 5169 6127
Size: 416 53 83 399 24 2554 1198 958
Cuts at: 6127 442
Size: 1753
Fragments arranged by size:
2554 1753 1198 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3592 3631 3592
Size: 39 7399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2276 2338 2412 2517 2525 2609 2735
Size: 833 204 62 74 105 8 84 126
Cuts at: 2735 2783 3055 4064 6098 1239
Size: 48 272 1009 2034 2579
Fragments arranged by size:
2579 2034 1009 833 272 204 126 105
84 74 62 48 8
Bpu10I CC'TnA_GC
Cuts at: 3934 4080 3934
Size: 146 7292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5563 6428 6726 5563
Size: 865 298 6275
Fragments arranged by size:
6275 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2372 3437 6101 882
Size: 617 873 1065 2664 2219
Fragments arranged by size:
2664 2219 1065 873 617
BsaAI yAC'GTr
Cuts at: 493 1204 5229 493
Size: 711 4025 2702
Fragments arranged by size:
4025 2702 711
BsaBI GATnn'nnATC
Cuts at: 3629 7432 3629
Size: 3803 3635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3166 5245 5627 6262
Size: 53 83 186 936 1633 2079 382 635
Cuts at: 6262 275
Size: 1451
Fragments arranged by size:
2079 1633 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5871 6269 6543 6690 7101 5871
Size: 398 274 147 411 6208
Fragments arranged by size:
6208 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4640 4670 1178
Size: 30 165 30 3237 30 3946
Fragments arranged by size:
3946 3237 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2057 2114 2273 2471 2572 3710 3922
Size: 838 33 57 159 198 101 1138 212
Cuts at: 3922 4709 1186
Size: 787 3915
Fragments arranged by size:
3915 1138 838 787 212 198 159 101
57 33
BseYI C'CCAG_C
Cuts at: 2244 2728 2809 2841 3364 3945 6641 7137 2244
Size: 484 81 32 523 581 2696 496 2545
Fragments arranged by size:
2696 2545 581 523 496 484 81 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2912 3038 3234 3362 1163
Size: 573 1176 126 196 128 5239
Fragments arranged by size:
5239 1176 573 196 128 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4888 5649 6677 1540
Size: 275 186 2887 761 1028 2301
Fragments arranged by size:
2887 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3735 5499 5584 6655 3735
Size: 1764 85 1071 4518
Fragments arranged by size:
4518 1764 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3348 4949 5042 3203
Size: 145 1601 93 5599
Fragments arranged by size:
5599 1601 145 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2473 2627 2637 3107 3373 3687 3701
Size: 399 683 154 10 470 266 314 14
Cuts at: 3701 3735 4103 4338 4473 4757 5499 5584 6655
Size: 34 368 235 135 284 742 85 1071
Cuts at: 6655 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 683 470 399 368 314
284 266 235 154 135 85 34 14
10
BspEI T'CCGG_A
Cuts at: 7101 7101
Size: 7438
BspHI T'CATG_A
Cuts at: 5327 7057 5327
Size: 1730 5708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2379 4128 4793 844
Size: 1535 1749 665 3489
Fragments arranged by size:
3489 1749 1535 665
BsrBI CCG'CTC
Cuts at: 1394 1823 4630 5325 1394
Size: 429 2807 695 3507
Fragments arranged by size:
3507 2807 695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5933 6107 65
Size: 1183 4685 174 1396
Fragments arranged by size:
4685 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2104 2722 2779 3740 4564 6082 6269
Size: 110 294 618 57 961 824 1518 187
Cuts at: 6269 1700
Size: 2869
Fragments arranged by size:
2869 1518 961 824 618 294 187 110
57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7438
BssHII G'CGCG_C
Cuts at: 1925 2240 1925
Size: 315 7123
BssSI C'ACGA_G
Cuts at: 5498 6510 5498
Size: 1012 6426
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2725 2998 794
Size: 1931 273 5234
Fragments arranged by size:
5234 1931 273
BstBI TT'CG_AA
Cuts at: 3034 6242 3034
Size: 3208 4230
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7438
BstXI CCAn_nnnn'nTGG
Cuts at: 4330 4735 4330
Size: 405 7033
BstYI r'GATC_y
Cuts at: 1972 2436 2551 3570 3630 4260 4768 5135 5520
Size: 464 115 1019 60 630 508 367 385
Cuts at: 5520 5537 6989 7075 7087 7433 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1019 630 508 464 385 367
346 115 86 60 17 12
Bsu36I CC'TnA_GG
Cuts at: 2445 2817 3693 2445
Size: 372 876 6190
Fragments arranged by size:
6190 876 372
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5947
Fragments arranged by size:
5947 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6699
Fragments arranged by size:
6699 683 56
BtsI GCAGTG_nn'
Cuts at: 2996 3333 3345 4545 4954 5748 5768 2996
Size: 337 12 1200 409 794 20 4666
Fragments arranged by size:
4666 1200 794 409 337 20 12
ClaI AT'CG_AT
Cuts at: 1554 2832 1554
Size: 1278 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3948 3983 4048 4083 529
Size: 35 3384 35 65 35 3884
Fragments arranged by size:
3884 3384 65 35 35 35
DraI TTT'AAA
Cuts at: 4742 4742
Size: 7438
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3830 6445 809
Size: 507 2514 2615 1802
Fragments arranged by size:
2615 2514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2848 3738
Size: 54 1425 50 275 18 567 451 890
Cuts at: 3738 4149 4725 4885 5774 7403 8
Size: 411 576 160 889 1629 43
Fragments arranged by size:
1629 1425 890 889 576 567 451 411
275 160 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 3143 3359 5368 1353
Size: 1253 537 216 2009 3423
Fragments arranged by size:
3423 2009 1253 537 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 6025 6399 6545 387
Size: 1064 138 2405 2031 374 146 1280
Fragments arranged by size:
2405 2031 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2276 2338 2412 2517 2525 2609
Size: 393 440 204 62 74 105 8 84
Cuts at: 2609 2735 2783 3055 4064 4621 4637 5501 6098
Size: 126 48 272 1009 557 16 864 597
Cuts at: 6098 6885 7153 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1009 864 787 597 557 440 393
272 268 204 126 105 84 74 62
48 16 8
EcoO109I rG'GnC_Cy
Cuts at: 2280 2470 2509 2813 2821 3085 3103 3145 3217
Size: 190 39 304 8 264 18 42 72
Cuts at: 3217 3401 3697 3698 3832 2280
Size: 184 296 1 134 5886
Fragments arranged by size:
5886 304 296 264 190 184 134 72
42 39 18 8 1
EcoRI G'AATT_C
Cuts at: 2649 4747 5125 2649
Size: 2098 378 4962
Fragments arranged by size:
4962 2098 378
EcoRV GAT'ATC
Cuts at: 3160 3160
Size: 7438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4702 4702
Size: 7438
FspI TGC'GCA
Cuts at: 3770 5944 3770
Size: 2174 5264
HaeII r_GCGC'y
Cuts at: 1678 3043 3169 6585 1678
Size: 1365 126 3416 2531
Fragments arranged by size:
3416 2531 1365 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2428 2460 2969 3001 3436
Size: 32 135 32 856 32 509 32 435
Cuts at: 3436 3468 4284 4316 6079 6111 6153 6185 1373
Size: 32 816 32 1763 32 42 32 2626
Fragments arranged by size:
2626 1763 856 816 509 435 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4782 5028 669
Size: 988 281 2844 246 3079
Fragments arranged by size:
3079 2844 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7438
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KasI G'GCGC_C
Cuts at: 3165 3165
Size: 7438
KpnI G_GTAC'C
Cuts at: 2166 3747 4763 2166
Size: 1581 1016 4841
Fragments arranged by size:
4841 1581 1016
MfeI C'AATT_G
Cuts at: 5037 5037
Size: 7438
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3297 3951 6552 6736 1305
Size: 1992 654 2601 184 2007
Fragments arranged by size:
2601 2007 1992 654 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 4727 10
Size: 54 1425 343 567 2328 2721
Fragments arranged by size:
2721 2328 1425 567 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 2867 3338 3989 4187 4223 4328
Size: 648 1521 180 471 651 198 36 105
Cuts at: 4328 4464 4733 5396 5755 5914 518
Size: 136 269 663 359 159 2042
Fragments arranged by size:
2042 1521 663 651 648 471 359 269
198 180 159 136 105 36
MspA1I CmG'CkG
Cuts at: 1606 2756 4282 4407 4609 4854 5531 6679 6924
Size: 1150 1526 125 202 245 677 1148 245
Cuts at: 6924 1606
Size: 2120
Fragments arranged by size:
2120 1526 1150 1148 677 245 245 202
125
NaeI GCC'GGC
Cuts at: 2106 4566 2106
Size: 2460 4978
NarI GG'CG_CC
Cuts at: 3166 3166
Size: 7438
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5947
Fragments arranged by size:
5947 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7438
NgoMIV G'CCGG_C
Cuts at: 2104 4564 2104
Size: 2460 4978
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7438
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NsiI A_TGCA'T
Cuts at: 3205 3205
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 4504 4796 6341 1132
Size: 97 3275 292 1545 2229
Fragments arranged by size:
3275 2229 1545 292 97
PasI CC'CwG_GG
Cuts at: 1348 2512 4244 1348
Size: 1164 1732 4542
Fragments arranged by size:
4542 1732 1164
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3220 3961 1108
Size: 2112 741 4585
Fragments arranged by size:
4585 2112 741
PfoI T'CCnGG_A
Cuts at: 2712 2946 3636 4041 4263 2712
Size: 234 690 405 222 5887
Fragments arranged by size:
5887 690 405 234 222
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 5507 5539 7020 7052 2867
Size: 32 2608 32 1481 32 3253
Fragments arranged by size:
3253 2608 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2813 3217 3401 3832 2813
Size: 404 184 431 6419
Fragments arranged by size:
6419 431 404 184
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 3663 5008 3663
Size: 1345 6093
PspOMI G'GGCC_C
Cuts at: 2469 3103 3697 2469
Size: 634 594 6210
Fragments arranged by size:
6210 634 594
PstI C_TGCA'G
Cuts at: 830 2019 2190 2533 2748 2957 3123 3340 4255
Size: 1189 171 343 215 209 166 217 915
Cuts at: 4255 4790 830
Size: 535 3478
Fragments arranged by size:
3478 1189 915 535 343 217 215 209
171 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
SalI G'TCGA_C
Cuts at: 1936 4780 1936
Size: 2844 4594
SanDI GG'GwC_CC
Cuts at: 3832 3832
Size: 7438
SapI GCTCTTCn'nnn_
Cuts at: 2606 3143 2606
Size: 537 6901
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 5686 7398 5686
Size: 1712 5726
SfcI C'TryA_G
Cuts at: 826 1045 2015 2186 2529 2744 2953 3119 3336
Size: 219 970 171 343 215 209 166 217
Cuts at: 3336 3650 4251 4786 5921 6602 6793 826
Size: 314 601 535 1135 681 191 1471
Fragments arranged by size:
1471 1135 970 681 601 535 343 314
219 217 215 209 191 171 166
SfiI GGCCn_nnn'nGGCC
Cuts at: 4574 4574
Size: 7438
SfoI GGC'GCC
Cuts at: 3167 3167
Size: 7438
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 2160 3941 4765 1872
Size: 288 1781 824 4545
Fragments arranged by size:
4545 1781 824 288
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5542 6443 6705 820
Size: 197 926 3599 901 262 1553
Fragments arranged by size:
3599 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4504 4796 1132
Size: 3372 292 3774
Fragments arranged by size:
3774 3372 292
SspI AAT'ATT
Cuts at: 5 52 5362 5
Size: 47 5310 2081
Fragments arranged by size:
5310 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7438
StyI C'CwwG_G
Cuts at: 513 1065 2004 2349 4677 4877 513
Size: 552 939 345 2328 200 3074
Fragments arranged by size:
3074 2328 939 552 345 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2236 2466 2855 3660 3905 3972 4130
Size: 1255 656 230 389 805 245 67 158
Cuts at: 4130 4217 4465 4476 4537 5465 5482 5635 5820
Size: 87 248 11 61 928 17 153 185
Cuts at: 5820 6265 325
Size: 445 1498
Fragments arranged by size:
1498 1255 928 805 656 445 389 248
245 230 185 158 153 87 67 61
17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3459 4363 5684
Size: 274 80 33 51 1443 1482 904 1321
Cuts at: 5684 7396 96
Size: 1712 138
Fragments arranged by size:
1712 1482 1443 1321 904 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4640 4672 7398 7430 4640
Size: 32 2726 32 4648
Fragments arranged by size:
4648 2726 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7438
XbaI T'CTAG_A
Cuts at: 3454 4774 3454
Size: 1320 6118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3850 3850
Size: 7438
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7438
XmaI C'CCGG_G
Cuts at: 1870 2158 3939 4763 1870
Size: 288 1781 824 4545
Fragments arranged by size:
4545 1781 824 288
XmnI GAAnn'nnTTC
Cuts at: 2661 5567 2661
Size: 2906 4532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5246 276
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoNI FseI FspAI NruI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SexAI SrfI
SwaI