(Circular) (Six-base) MAPSORT of: pFN21AB8556.seq Check: 3568 from: 1 to: 7435
pFN21AB8556 7435 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3402 5245 278
Size: 53 83 186 936 1866 1843 2468
Fragments arranged by size:
2468 1866 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4778 1937
Size: 2841 4594
Acc65I G'GTAC_C
Cuts at: 4756 4756
Size: 7435
AclI AA'CG_TT
Cuts at: 1563 5562 5935 1563
Size: 3999 373 3063
Fragments arranged by size:
3999 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2876 3623 4219 4239 5498 6882 7150 1632
Size: 1244 747 596 20 1259 1384 268 1917
Fragments arranged by size:
1917 1384 1259 1244 747 596 268 20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7238
AflIII A'CryG_T
Cuts at: 1203 6334 7311 1203
Size: 5131 977 1327
Fragments arranged by size:
5131 1327 977
AgeI A'CCGG_T
Cuts at: 6266 6266
Size: 7435
AhdI GACnn_n'nnGTC
Cuts at: 3478 6164 3478
Size: 2686 4749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7435
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4171 4203 4171
Size: 32 7403
AlwNI CAG_nnn'CTG
Cuts at: 2505 4612 5178 6750 2505
Size: 2107 566 1572 3190
Fragments arranged by size:
3190 2107 1572 566
ApaI G_GGCC'C
Cuts at: 2959 2959
Size: 7435
ApaLI G'TGCA_C
Cuts at: 2250 5492 6648 2250
Size: 3242 1156 3037
Fragments arranged by size:
3242 3037 1156
ApoI r'AATT_y
Cuts at: 1493 2050 2287 2309 2393 4744 4970 5122 7231
Size: 557 237 22 84 2351 226 152 2109
Cuts at: 7231 1493
Size: 1697
Fragments arranged by size:
2351 2109 1697 557 237 226 152 84
22
AseI AT'TA_AT
Cuts at: 160 1032 5989 160
Size: 872 4957 1606
Fragments arranged by size:
4957 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
AvaI C'yCGr_G
Cuts at: 1870 1943 2920 3317 4427 4454 4567 4760 1870
Size: 73 977 397 1110 27 113 193 4545
Fragments arranged by size:
4545 1110 977 397 193 113 73 27
BamHI G'GATC_C
Cuts at: 3856 4072 4765 5132 3856
Size: 216 693 367 6159
Fragments arranged by size:
6159 693 367 216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2666 2815 3722 4756
Size: 325 217 627 224 655 149 907 1034
Cuts at: 4756 6211 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1034 907 655 627 325 224
217 149
BanII G_rGCy'C
Cuts at: 1391 2587 2601 2959 3318 3718 4754 1391
Size: 1196 14 358 359 400 1036 4072
Fragments arranged by size:
4072 1196 1036 400 359 358 14
BbeI G_GCGC'C
Cuts at: 2015 2670 2015
Size: 655 6780
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2657 2864 3495 3879 928
Size: 971 758 207 631 384 4484
Fragments arranged by size:
4484 971 758 631 384 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2525 2559 5626 5660 1903
Size: 34 588 34 3067 34 3678
Fragments arranged by size:
3678 3067 588 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5327 6537 5327
Size: 1210 6225
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7435
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2366 6046
Size: 107 122 71 631 605 337 357 3680
Cuts at: 6046 136
Size: 1525
Fragments arranged by size:
3680 1525 631 605 357 337 122 107
71
BglII A'GATC_T
Cuts at: 2907 7430 2907
Size: 4523 2912
BlpI GC'TnA_GC
Cuts at: 3392 4709 4852 3392
Size: 1317 143 5975
Fragments arranged by size:
5975 1317 143
Bme1580I G_kGCm'C
Cuts at: 1790 2254 2538 2607 2820 2959 5496 6652 1790
Size: 464 284 69 213 139 2537 1156 2573
Fragments arranged by size:
2573 2537 1156 464 284 213 139 69
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 2818 3181
Size: 416 53 83 399 24 957 444 363
Cuts at: 3181 5166 6124 442
Size: 1985 958 1753
Fragments arranged by size:
1985 1753 958 957 444 416 399 363
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2463 2609 3569 3707 4129 6095 1239
Size: 1224 146 960 138 422 1966 2579
Fragments arranged by size:
2579 1966 1224 960 422 146 138
Bpu10I CC'TnA_GC
Cuts at: 3749 3749
Size: 7435
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 2768 3042 3324 5560 6425 6723 2296
Size: 472 274 282 2236 865 298 3008
Fragments arranged by size:
3008 2236 865 472 298 282 274
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6098 882
Size: 617 4599 2219
Fragments arranged by size:
4599 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2884 5226 493
Size: 711 1680 2342 2702
Fragments arranged by size:
2702 2342 1680 711
BsaBI GATnn'nnATC
Cuts at: 7429 7429
Size: 7435
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2667 3399 3759
Size: 53 83 186 936 479 655 732 360
Cuts at: 3759 5242 5624 6259 275
Size: 1483 382 635 1451
Fragments arranged by size:
1483 1451 936 732 655 635 479 382
360 186 83 53
BsaWI w'CCGG_w
Cuts at: 3853 5868 6266 6540 6687 7098 3853
Size: 2015 398 274 147 411 4190
Fragments arranged by size:
4190 2015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2436 2466 1178
Size: 30 165 30 1033 30 6147
Fragments arranged by size:
6147 1033 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3045 4144 4267 4350 4525 4616 1186
Size: 1859 1099 123 83 175 91 4005
Fragments arranged by size:
4005 1859 1099 175 123 91 83
BseYI C'CCAG_C
Cuts at: 2031 3543 3566 3648 4379 6638 7134 2031
Size: 1512 23 82 731 2259 496 2332
Fragments arranged by size:
2332 2259 1512 731 496 82 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3287 1163
Size: 573 1551 5311
Fragments arranged by size:
5311 1551 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4885 5646 6674 1540
Size: 275 186 2884 761 1028 2301
Fragments arranged by size:
2884 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2254 3318 3708 5496 5581 6652 2254
Size: 1064 390 1788 85 1071 3037
Fragments arranged by size:
3037 1788 1071 1064 390 85
BsmI GAATG_Cn'
Cuts at: 3558 4946 5039 3558
Size: 1388 93 5954
Fragments arranged by size:
5954 1388 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3766 3766
Size: 7435
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2254 2538 2587 2601 2607 2820 2959
Size: 399 464 284 49 14 6 213 139
Cuts at: 2959 3318 3708 3718 4754 5496 5581 6652 1391
Size: 359 390 10 1036 742 85 1071 2174
Fragments arranged by size:
2174 1071 1036 742 464 399 390 359
284 213 139 85 49 14 10 6
BspEI T'CCGG_A
Cuts at: 3853 7098 3853
Size: 3245 4190
BspHI T'CATG_A
Cuts at: 2466 5324 7054 2466
Size: 2858 1730 2847
Fragments arranged by size:
2858 2847 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2068 4363 4790 844
Size: 1224 2295 427 3489
Fragments arranged by size:
3489 2295 1224 427
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 2715 5322 1394
Size: 429 194 698 2607 3507
Fragments arranged by size:
3507 2607 698 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 4603 5930 6104 65
Size: 1183 3355 1327 174 1396
Fragments arranged by size:
3355 1396 1327 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6079 6266 1700
Size: 110 4269 187 2869
Fragments arranged by size:
4269 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7435
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7435
BssSI C'ACGA_G
Cuts at: 5495 6507 5495
Size: 1012 6423
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3847 794
Size: 3053 4382
BstBI TT'CG_AA
Cuts at: 4103 6239 4103
Size: 2136 5299
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7435
BstXI CCAn_nnnn'nTGG
Cuts at: 2572 2572
Size: 7435
BstYI r'GATC_y
Cuts at: 1972 2907 3856 3970 4072 4168 4186 4765 5132
Size: 935 949 114 102 96 18 579 367
Cuts at: 5132 5517 5534 6986 7072 7084 7430 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 949 935 579 385 367 346
114 102 96 86 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2728 2820 3718 3792 4012 4364
Size: 552 939 724 92 898 74 220 352
Cuts at: 4364 513
Size: 3584
Fragments arranged by size:
3584 939 898 724 552 352 220 92
74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4687 487
Size: 683 56 3461 3235
Fragments arranged by size:
3461 3235 683 56
BtsI GCAGTG_nn'
Cuts at: 4951 5745 5765 4951
Size: 794 20 6621
Fragments arranged by size:
6621 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7435
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7400
DraI TTT'AAA
Cuts at: 3898 4315 4739 3898
Size: 417 424 6594
Fragments arranged by size:
6594 424 417
DraIII CAC_nnn'GTG
Cuts at: 2258 2575 2258
Size: 317 7118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4526 6442 809
Size: 507 3210 1916 1802
Fragments arranged by size:
3210 1916 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2260 2562
Size: 54 1425 50 275 18 351 79 302
Cuts at: 2562 2890 2961 3741 4698 4882 5771 7400 8
Size: 328 71 780 957 184 889 1629 43
Fragments arranged by size:
1629 1425 957 889 780 351 328 302
275 184 79 71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4882 1537
Size: 275 3070 4090
Fragments arranged by size:
4090 3070 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3231 5365 1353
Size: 1878 2134 3423
Fragments arranged by size:
3423 2134 1878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2599 4658 6022 6396 6542 387
Size: 1064 138 1010 2059 1364 374 146 1280
Fragments arranged by size:
2059 1364 1280 1064 1010 374 146 138
EcoICRI GAG'CTC
Cuts at: 3316 3316
Size: 7435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2463 2609 2876 3569 3623 3707 4129
Size: 393 831 146 267 693 54 84 422
Cuts at: 4129 4219 4239 5498 6095 6882 7150 1239
Size: 90 20 1259 597 787 268 1524
Fragments arranged by size:
1524 1259 831 787 693 597 422 393
268 267 146 90 84 54 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2591 2591
Size: 7435
EcoO109I rG'GnC_Cy
Cuts at: 2509 2956 3985 4203 4432 4501 4687 2509
Size: 447 1029 218 229 69 186 5257
Fragments arranged by size:
5257 1029 447 229 218 186 69
EcoRI G'AATT_C
Cuts at: 4744 5122 4744
Size: 378 7057
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2141 2926 2141
Size: 785 6650
FspI TGC'GCA
Cuts at: 5941 5941
Size: 7435
HaeII r_GCGC'y
Cuts at: 1678 2015 2670 6582 1678
Size: 337 655 3912 2531
Fragments arranged by size:
3912 2531 655 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2436 2468 3464 3496 4571
Size: 32 135 32 864 32 996 32 1075
Cuts at: 4571 4603 6076 6108 6150 6182 1373
Size: 32 1473 32 42 32 2626
Fragments arranged by size:
2626 1473 1075 996 864 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 4779 5025 669
Size: 988 281 1671 1170 246 3079
Fragments arranged by size:
3079 1671 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7435
HpaI GTT'AAC
Cuts at: 5025 5025
Size: 7435
KasI G'GCGC_C
Cuts at: 2011 2666 2011
Size: 655 6780
KpnI G_GTAC'C
Cuts at: 4760 4760
Size: 7435
MfeI C'AATT_G
Cuts at: 5034 5034
Size: 7435
MluI A'CGCG_T
Cuts at: 7311 7311
Size: 7435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3492 6549 6733 1305
Size: 2187 3057 184 2007
Fragments arranged by size:
3057 2187 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2262 2564 2892 3743 4700
Size: 54 1425 343 430 302 328 851 957
Cuts at: 4700 10
Size: 2745
Fragments arranged by size:
2745 1425 957 851 430 343 328 302
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2950 5393 5752 5911 518
Size: 648 1784 2443 359 159 2042
Fragments arranged by size:
2443 2042 1784 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3547 3580 3652 4383 4467 4612 4851 5528
Size: 1941 33 72 731 84 145 239 677
Cuts at: 5528 6676 6921 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1941 1148 731 677 245 239 145
84 72 33
NarI GG'CG_CC
Cuts at: 2012 2667 2012
Size: 655 6780
NcoI C'CATG_G
Cuts at: 513 1065 2004 3718 3792 513
Size: 552 939 1714 74 4156
Fragments arranged by size:
4156 1714 939 552 74
NdeI CA'TA_TG
Cuts at: 387 2951 387
Size: 2564 4871
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7435
NotI GC'GGCC_GC
Cuts at: 4882 4882
Size: 7435
NspI r_CATG'y
Cuts at: 1132 1229 2299 3391 4726 4793 6338 1132
Size: 97 1070 1092 1335 67 1545 2229
Fragments arranged by size:
2229 1545 1335 1092 1070 97 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7435
PciI A'CATG_T
Cuts at: 6334 6334
Size: 7435
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4013 4262 1108
Size: 2905 249 4281
Fragments arranged by size:
4281 2905 249
PmeI GTTT'AAAC
Cuts at: 4739 4739
Size: 7435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5504 5536 7017 7049 5504
Size: 32 1481 32 5890
Fragments arranged by size:
5890 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2509 3985 4203 4501 4687 2509
Size: 1476 218 298 186 5257
Fragments arranged by size:
5257 1476 298 218 186
PshAI GACnn'nnGTC
Cuts at: 1613 3171 1613
Size: 1558 5877
PsiI TTA'TAA
Cuts at: 5005 5005
Size: 7435
PspOMI G'GGCC_C
Cuts at: 2955 2955
Size: 7435
PspXI vC'TCGA_Gb
Cuts at: 3317 3317
Size: 7435
PstI C_TGCA'G
Cuts at: 830 2356 4543 4787 830
Size: 1526 2187 244 3478
Fragments arranged by size:
3478 2187 1526 244
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7435
PvuII CAG'CTG
Cuts at: 3547 3580 3652 4612 3547
Size: 33 72 960 6370
Fragments arranged by size:
6370 960 72 33
SacI G_AGCT'C
Cuts at: 3318 3318
Size: 7435
SalI G'TCGA_C
Cuts at: 1936 4777 1936
Size: 2841 4594
SanDI GG'GwC_CC
Cuts at: 3985 4501 3985
Size: 516 6919
SbfI CC_TGCA'GG
Cuts at: 4787 4787
Size: 7435
ScaI AGT'ACT
Cuts at: 5683 7395 5683
Size: 1712 5723
SexAI A'CCwGG_T
Cuts at: 3699 3699
Size: 7435
SfcI C'TryA_G
Cuts at: 826 1045 2352 4539 4783 5918 6599 6790 826
Size: 219 1307 2187 244 1135 681 191 1471
Fragments arranged by size:
2187 1471 1307 1135 681 244 219 191
SfoI GGC'GCC
Cuts at: 2013 2668 2013
Size: 655 6780
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7435
SmaI CCC'GGG
Cuts at: 1872 4762 1872
Size: 2890 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2275 2783 3057 3317 3339 5539
Size: 197 926 332 508 274 260 22 2200
Cuts at: 5539 6440 6702 820
Size: 901 262 1553
Fragments arranged by size:
2200 1553 926 901 508 332 274 262
260 197 22
SnaBI TAC'GTA
Cuts at: 493 5226 493
Size: 4733 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7435
SphI G_CATG'C
Cuts at: 1132 2299 4793 1132
Size: 1167 2494 3774
Fragments arranged by size:
3774 2494 1167
SspI AAT'ATT
Cuts at: 5 52 3206 5359 5
Size: 47 3154 2153 2081
Fragments arranged by size:
3154 2153 2081 47
StuI AGG'CCT
Cuts at: 1517 4559 1517
Size: 3042 4393
StyI C'CwwG_G
Cuts at: 513 1065 2004 2161 3718 3792 4281 4874 513
Size: 552 939 157 1557 74 489 593 3074
Fragments arranged by size:
3074 1557 939 593 552 489 157 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2800 2876 2945 3749 5462 5479 5632
Size: 1255 1220 76 69 804 1713 17 153
Cuts at: 5632 5817 6262 325
Size: 185 445 1498
Fragments arranged by size:
1713 1498 1255 1220 804 445 185 153
76 69 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4391 5681 7393
Size: 274 80 33 51 1443 2414 1290 1712
Cuts at: 7393 96
Size: 138
Fragments arranged by size:
2414 1712 1443 1290 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7395 7427 7395
Size: 32 7403
Tth111I GACn'n_nGTC
Cuts at: 1316 2450 3077 1316
Size: 1134 627 5674
Fragments arranged by size:
5674 1134 627
XbaI T'CTAG_A
Cuts at: 4771 4771
Size: 7435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2805 3707 2805
Size: 902 6533
XhoI C'TCGA_G
Cuts at: 1943 3317 1943
Size: 1374 6061
XmaI C'CCGG_G
Cuts at: 1870 4760 1870
Size: 2890 4545
XmnI GAAnn'nnTTC
Cuts at: 5564 5564
Size: 7435
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3400 5243 276
Size: 53 83 186 936 1866 1843 2468
Fragments arranged by size:
2468 1866 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PsrI RsrII SacII
SapI SfiI SrfI SwaI