Restriction map for
pFN21AB8550
(Circular) (Six-base) MAPSORT of: pFN21AB8550.seq Check: 7893 from: 1 to: 7423
pFN21AB8550 7423 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2506 3022 2506
Size: 516 6907
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5233 278
Size: 53 83 186 936 3697 2468
Fragments arranged by size:
3697 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2442 4766 1937
Size: 505 2324 4594
Fragments arranged by size:
4594 2324 505
Acc65I G'GTAC_C
Cuts at: 4744 4744
Size: 7423
AclI AA'CG_TT
Cuts at: 1563 5550 5923 1563
Size: 3987 373 3063
Fragments arranged by size:
3987 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 3667 4006 5486 6870 7138 1632
Size: 725 1310 339 1480 1384 268 1917
Fragments arranged by size:
1917 1480 1384 1310 725 339 268
AfeI AGC'GCT
Cuts at: 4402 4402
Size: 7423
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7226
AflIII A'CryG_T
Cuts at: 1203 6322 7299 1203
Size: 5119 977 1327
Fragments arranged by size:
5119 1327 977
AgeI A'CCGG_T
Cuts at: 2033 6254 2033
Size: 4221 3202
AhdI GACnn_n'nnGTC
Cuts at: 6152 6152
Size: 7423
AleI CACnn'nnGTG
Cuts at: 1166 2843 1166
Size: 1677 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2034 1184
Size: 850 6573
AlwNI CAG_nnn'CTG
Cuts at: 2501 2578 2923 5166 6738 2501
Size: 77 345 2243 1572 3186
Fragments arranged by size:
3186 2243 1572 345 77
ApaI G_GGCC'C
Cuts at: 2314 2314
Size: 7423
ApaLI G'TGCA_C
Cuts at: 5480 6636 5480
Size: 1156 6267
ApoI r'AATT_y
Cuts at: 1493 2435 2729 4732 4958 5110 7219 1493
Size: 942 294 2003 226 152 2109 1697
Fragments arranged by size:
2109 2003 1697 942 294 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5977 160
Size: 872 4945 1606
Fragments arranged by size:
4945 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7423
AvaI C'yCGr_G
Cuts at: 1870 1943 2829 3402 4748 1870
Size: 73 886 573 1346 4545
Fragments arranged by size:
4545 1346 886 573 73
AvrII C'CTAG_G
Cuts at: 2041 2041
Size: 7423
BamHI G'GATC_C
Cuts at: 4753 5120 4753
Size: 367 7056
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2744 4744 6199 618
Size: 325 217 627 270 687 2000 1455 1842
Fragments arranged by size:
2000 1842 1455 687 627 325 270 217
BanII G_rGCy'C
Cuts at: 1391 2160 2314 3025 3403 4742 1391
Size: 769 154 711 378 1339 4072
Fragments arranged by size:
4072 1339 769 711 378 154
BbeI G_GCGC'C
Cuts at: 2748 2748
Size: 7423
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3580 928
Size: 971 1681 4771
Fragments arranged by size:
4771 1681 971
BbvCI CC'TCA_GC
Cuts at: 2907 4412 2907
Size: 1505 5918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5614 5648 1903
Size: 34 50 34 3593 34 3678
Fragments arranged by size:
3678 3593 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2485 2549 5315 6525 2485
Size: 64 2766 1210 3383
Fragments arranged by size:
3383 2766 1210 64
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7423
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3905 6034 136
Size: 107 122 71 631 605 2233 2129 1525
Fragments arranged by size:
2233 2129 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7418 7418
Size: 7423
BlpI GC'TnA_GC
Cuts at: 4840 4840
Size: 7423
Bme1580I G_kGCm'C
Cuts at: 1790 2314 5484 6640 1790
Size: 524 3170 1156 2573
Fragments arranged by size:
3170 2573 1156 524
BmgBI CAC'GTC
Cuts at: 1318 2320 1318
Size: 1002 6421
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2227 5154 6112
Size: 416 53 83 399 24 810 2927 958
Cuts at: 6112 442
Size: 1753
Fragments arranged by size:
2927 1753 958 810 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 3392 4178 6083 1239
Size: 1361 792 786 1905 2579
Fragments arranged by size:
2579 1905 1361 792 786
Bpu10I CC'TnA_GC
Cuts at: 2907 4412 2907
Size: 1505 5918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3254 5548 6413 6711 3254
Size: 2294 865 298 3966
Fragments arranged by size:
3966 2294 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2826 2851 3399 6086 882
Size: 617 1327 25 548 2687 2219
Fragments arranged by size:
2687 2219 1327 617 548 25
BsaAI yAC'GTr
Cuts at: 493 1204 3106 3352 5214 493
Size: 711 1902 246 1862 2702
Fragments arranged by size:
2702 1902 1862 711 246
BsaBI GATnn'nnATC
Cuts at: 3011 3548 7417 3011
Size: 537 3869 3017
Fragments arranged by size:
3869 3017 537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2745 3082 3855 5230
Size: 53 83 186 936 1212 337 773 1375
Cuts at: 5230 5612 6247 275
Size: 382 635 1451
Fragments arranged by size:
1451 1375 1212 936 773 635 382 337
186 83 53
BsaWI w'CCGG_w
Cuts at: 2033 2559 5856 6254 6528 6675 7086 2033
Size: 526 3297 398 274 147 411 2370
Fragments arranged by size:
3297 2370 526 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3432 3462 3642 3672 1178
Size: 30 165 30 2029 30 180 30 4929
Fragments arranged by size:
4929 2029 180 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 3877 4121 1186
Size: 1378 1313 244 4488
Fragments arranged by size:
4488 1378 1313 244
BseYI C'CCAG_C
Cuts at: 2963 2983 3194 6626 7122 2963
Size: 20 211 3432 496 3264
Fragments arranged by size:
3432 3264 496 211 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2014 3083 3972 1163
Size: 573 278 1069 889 4614
Fragments arranged by size:
4614 1069 889 573 278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2461 4873 5634 6662 1540
Size: 275 186 460 2412 761 1028 2301
Fragments arranged by size:
2412 2301 1028 761 460 275 186
BsiHKAI G_wGCw'C
Cuts at: 3025 5484 5569 6640 3025
Size: 2459 85 1071 3808
Fragments arranged by size:
3808 2459 1071 85
BsmI GAATG_Cn'
Cuts at: 2965 4934 5027 2965
Size: 1969 93 5361
Fragments arranged by size:
5361 1969 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2314 3025 3403 4742 5484 5569
Size: 399 370 154 711 378 1339 742 85
Cuts at: 5569 6640 1391
Size: 1071 2174
Fragments arranged by size:
2174 1339 1071 742 711 399 378 370
154 85
BspEI T'CCGG_A
Cuts at: 7086 7086
Size: 7423
BspHI T'CATG_A
Cuts at: 3552 5312 7042 3552
Size: 1760 1730 3933
Fragments arranged by size:
3933 1760 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2506 3022 4778 844
Size: 1662 516 1756 3489
Fragments arranged by size:
3489 1756 1662 516
BsrBI CCG'CTC
Cuts at: 1394 1823 2596 5310 1394
Size: 429 773 2714 3507
Fragments arranged by size:
3507 2714 773 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3304 4245 4423 5918 6092 65
Size: 1183 2056 941 178 1495 174 1396
Fragments arranged by size:
2056 1495 1396 1183 941 178 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 6067 6254 1700
Size: 110 223 4034 187 2869
Fragments arranged by size:
4034 2869 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7423
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7423
BssSI C'ACGA_G
Cuts at: 5483 6495 5483
Size: 1012 6411
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3034 3341 794
Size: 2240 307 4876
Fragments arranged by size:
4876 2240 307
BstBI TT'CG_AA
Cuts at: 6227 6227
Size: 7423
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7423
BstXI CCAn_nnnn'nTGG
Cuts at: 3352 4213 4720 3352
Size: 861 507 6055
Fragments arranged by size:
6055 861 507
BstYI r'GATC_y
Cuts at: 1972 3253 3543 4004 4753 5120 5505 5522 6974
Size: 1281 290 461 749 367 385 17 1452
Cuts at: 6974 7060 7072 7418 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1281 749 461 385 367 346
290 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3921 4713 513
Size: 552 2856 792 3223
Fragments arranged by size:
3223 2856 792 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6684
Fragments arranged by size:
6684 683 56
BtsI GCAGTG_nn'
Cuts at: 2816 4299 4624 4939 5733 5753 2816
Size: 1483 325 315 794 20 4486
Fragments arranged by size:
4486 1483 794 325 315 20
ClaI AT'CG_AT
Cuts at: 1554 4670 1554
Size: 3116 4307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2722 2757 529
Size: 35 2158 35 5195
Fragments arranged by size:
5195 2158 35 35
DraI TTT'AAA
Cuts at: 4727 4727
Size: 7423
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6430 809
Size: 507 5114 1802
Fragments arranged by size:
5114 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2793 3906 4870
Size: 54 1425 50 275 18 963 1113 964
Cuts at: 4870 5759 7388 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1113 964 963 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4870 1537
Size: 275 3058 4090
Fragments arranged by size:
4090 3058 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2849 3451 3671 3702 5353 1353
Size: 1496 602 220 31 1651 3423
Fragments arranged by size:
3423 1651 1496 602 220 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6010 6384 6530 387
Size: 1064 138 4421 374 146 1280
Fragments arranged by size:
4421 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3023 3023
Size: 7423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2600 3392 3667 4006 4178 5486
Size: 393 725 243 792 275 339 172 1308
Cuts at: 5486 6083 6870 7138 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1308 792 787 725 597 393 339
275 268 243 172
EcoO109I rG'GnC_Cy
Cuts at: 2310 2311 2575 3828 3958 2310
Size: 1 264 1253 130 5775
Fragments arranged by size:
5775 1253 264 130 1
EcoRI G'AATT_C
Cuts at: 4732 5110 4732
Size: 378 7045
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4036 4036
Size: 7423
FspI TGC'GCA
Cuts at: 5929 5929
Size: 7423
HaeII r_GCGC'y
Cuts at: 1678 2748 4404 6570 1678
Size: 1070 1656 2166 2531
Fragments arranged by size:
2531 2166 1656 1070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2603 2635 3432 3464 3922
Size: 32 135 32 1031 32 797 32 458
Cuts at: 3922 3954 4387 4419 6064 6096 6138 6170 1373
Size: 32 433 32 1645 32 42 32 2626
Fragments arranged by size:
2626 1645 1031 797 458 433 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 2530 4767 5013 669
Size: 988 281 349 243 2237 246 3079
Fragments arranged by size:
3079 2237 988 349 281 246 243
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7423
HpaI GTT'AAC
Cuts at: 5013 5013
Size: 7423
KasI G'GCGC_C
Cuts at: 2744 2744
Size: 7423
KpnI G_GTAC'C
Cuts at: 4748 4748
Size: 7423
MfeI C'AATT_G
Cuts at: 5022 5022
Size: 7423
MluI A'CGCG_T
Cuts at: 7299 7299
Size: 7423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6537 6721 1305
Size: 5232 184 2007
Fragments arranged by size:
5232 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5601
Fragments arranged by size:
5601 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2843 3650 4211 5381 5740 5899 518
Size: 648 1677 807 561 1170 359 159 2042
Fragments arranged by size:
2042 1677 1170 807 648 561 359 159
MspA1I CmG'CkG
Cuts at: 1606 2377 2498 2767 2987 4189 4371 4593 4611
Size: 771 121 269 220 1202 182 222 18
Cuts at: 4611 4839 5516 6664 6909 1606
Size: 228 677 1148 245 2120
Fragments arranged by size:
2120 1202 1148 771 677 269 245 228
222 220 182 121 18
NarI GG'CG_CC
Cuts at: 2745 2745
Size: 7423
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6871
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7423
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7423
NotI GC'GGCC_GC
Cuts at: 4870 4870
Size: 7423
NspI r_CATG'y
Cuts at: 1132 1229 2123 2293 4781 6326 1132
Size: 97 894 170 2488 1545 2229
Fragments arranged by size:
2488 2229 1545 894 170 97
PasI CC'CwG_GG
Cuts at: 1348 2994 3787 1348
Size: 1646 793 4984
Fragments arranged by size:
4984 1646 793
PciI A'CATG_T
Cuts at: 6322 6322
Size: 7423
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7423
PfoI T'CCnGG_A
Cuts at: 2487 3498 4155 2487
Size: 1011 657 5755
Fragments arranged by size:
5755 1011 657
PmeI GTTT'AAAC
Cuts at: 4727 4727
Size: 7423
PmlI CAC'GTG
Cuts at: 3106 3352 3106
Size: 246 7177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4675 4707 5492 5524 7005 7037 4675
Size: 32 785 32 1481 32 5061
Fragments arranged by size:
5061 1481 785 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2575 3828 3958 2575
Size: 1253 130 6040
Fragments arranged by size:
6040 1253 130
PshAI GACnn'nnGTC
Cuts at: 1613 2525 1613
Size: 912 6511
PsiI TTA'TAA
Cuts at: 2654 4993 2654
Size: 2339 5084
PspOMI G'GGCC_C
Cuts at: 2310 2310
Size: 7423
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 7391
PstI C_TGCA'G
Cuts at: 830 2394 2481 3030 3178 4775 830
Size: 1564 87 549 148 1597 3478
Fragments arranged by size:
3478 1597 1564 549 148 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7423
PvuII CAG'CTG
Cuts at: 2377 2498 2767 2987 2377
Size: 121 269 220 6813
Fragments arranged by size:
6813 269 220 121
SacI G_AGCT'C
Cuts at: 3025 3025
Size: 7423
SalI G'TCGA_C
Cuts at: 1936 4765 1936
Size: 2829 4594
SapI GCTCTTCn'nnn_
Cuts at: 3451 3451
Size: 7423
SbfI CC_TGCA'GG
Cuts at: 4775 4775
Size: 7423
ScaI AGT'ACT
Cuts at: 5671 7383 5671
Size: 1712 5711
SfcI C'TryA_G
Cuts at: 826 1045 2218 2390 2477 3026 3174 4771 5906
Size: 219 1173 172 87 549 148 1597 1135
Cuts at: 5906 6587 6778 826
Size: 681 191 1471
Fragments arranged by size:
1597 1471 1173 1135 681 549 219 191
172 148 87
SfoI GGC'GCC
Cuts at: 2746 2746
Size: 7423
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7423
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7423
SmaI CCC'GGG
Cuts at: 1872 4750 1872
Size: 2878 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2829 3269 5527 6428 6690 820
Size: 197 926 886 440 2258 901 262 1553
Fragments arranged by size:
2258 1553 926 901 886 440 262 197
SnaBI TAC'GTA
Cuts at: 493 5214 493
Size: 4721 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7423
SphI G_CATG'C
Cuts at: 1132 4781 1132
Size: 3649 3774
SspI AAT'ATT
Cuts at: 5 52 5347 5
Size: 47 5295 2081
Fragments arranged by size:
5295 2081 47
StuI AGG'CCT
Cuts at: 1517 3422 1517
Size: 1905 5518
StyI C'CwwG_G
Cuts at: 513 1065 2041 3001 4257 4386 4704 4862 513
Size: 552 976 960 1256 129 318 158 3074
Fragments arranged by size:
3074 1256 976 960 552 318 158 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4270 5450 5467 5620 5805 6250 325
Size: 1255 2690 1180 17 153 185 445 1498
Fragments arranged by size:
2690 1498 1255 1180 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2865 4176 5669
Size: 274 80 33 51 1443 888 1311 1493
Cuts at: 5669 7381 96
Size: 1712 138
Fragments arranged by size:
1712 1493 1443 1311 888 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2806 2838 4183 4215 7383 7415 2806
Size: 32 1345 32 3168 32 2814
Fragments arranged by size:
3168 2814 1345 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7423
XbaI T'CTAG_A
Cuts at: 4759 4759
Size: 7423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2991 2991
Size: 7423
XhoI C'TCGA_G
Cuts at: 1943 2829 1943
Size: 886 6537
XmaI C'CCGG_G
Cuts at: 1870 4748 1870
Size: 2878 4545
XmnI GAAnn'nnTTC
Cuts at: 5552 5552
Size: 7423
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5231 276
Size: 53 83 186 936 3697 2468
Fragments arranged by size:
3697 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PspXI RsrII SacII SanDI SexAI
SfiI SrfI SwaI