Restriction map for
pFN21AB8549
(Circular) (Six-base) MAPSORT of: pFN21AB8549.seq Check: 1431 from: 1 to: 7675
pFN21AB8549 7675 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3208 3390 3831 4287 3208
Size: 182 441 456 6596
Fragments arranged by size:
6596 456 441 182
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5485 278
Size: 53 83 186 936 3949 2468
Fragments arranged by size:
3949 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5018 1937
Size: 3081 4594
Acc65I G'GTAC_C
Cuts at: 2454 4996 2454
Size: 2542 5133
AclI AA'CG_TT
Cuts at: 1563 5802 6175 1563
Size: 4239 373 3063
Fragments arranged by size:
4239 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 2742 2918 3770 3788 4262 4454 4559
Size: 824 286 176 852 18 474 192 105
Cuts at: 4559 4698 5738 7122 7390 1632
Size: 139 1040 1384 268 1917
Fragments arranged by size:
1917 1384 1040 852 824 474 286 268
192 176 139 105 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7478
AflIII A'CryG_T
Cuts at: 1203 2396 6574 7551 1203
Size: 1193 4178 977 1327
Fragments arranged by size:
4178 1327 1193 977
AgeI A'CCGG_T
Cuts at: 6506 6506
Size: 7675
AhdI GACnn_n'nnGTC
Cuts at: 6404 6404
Size: 7675
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7675
AlwNI CAG_nnn'CTG
Cuts at: 3271 3814 3853 3889 4630 5418 6990 3271
Size: 543 39 36 741 788 1572 3956
Fragments arranged by size:
3956 1572 788 741 543 39 36
ApaI G_GGCC'C
Cuts at: 2417 3342 4030 4369 4723 2417
Size: 925 688 339 354 5369
Fragments arranged by size:
5369 925 688 354 339
ApaLI G'TGCA_C
Cuts at: 3221 5732 6888 3221
Size: 2511 1156 4008
Fragments arranged by size:
4008 2511 1156
ApoI r'AATT_y
Cuts at: 1493 4984 5210 5362 7471 1493
Size: 3491 226 152 2109 1697
Fragments arranged by size:
3491 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6229 160
Size: 872 5197 1606
Fragments arranged by size:
5197 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7675
AvaI C'yCGr_G
Cuts at: 1870 1943 4887 5000 1870
Size: 73 2944 113 4545
Fragments arranged by size:
4545 2944 113 73
BamHI G'GATC_C
Cuts at: 2640 5005 5372 2640
Size: 2365 367 4943
Fragments arranged by size:
4943 2365 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2454 4266 4548 4996
Size: 325 217 627 462 205 1812 282 448
Cuts at: 4996 6451 618
Size: 1455 1842
Fragments arranged by size:
1842 1812 1455 627 462 448 325 282
217 205
BanII G_rGCy'C
Cuts at: 1391 2417 2602 2782 3330 3342 3507 3603 4030
Size: 1026 185 180 548 12 165 96 427
Cuts at: 4030 4369 4413 4710 4723 4994 1391
Size: 339 44 297 13 271 4072
Fragments arranged by size:
4072 1026 548 427 339 297 271 185
180 165 96 44 13 12
BbeI G_GCGC'C
Cuts at: 4270 4270
Size: 7675
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6704
BbvCI CC'TCA_GC
Cuts at: 4128 4128
Size: 7675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3663 3697 4850 4884 5866 5900 1903
Size: 34 1726 34 1153 34 982 34 3678
Fragments arranged by size:
3678 1726 1153 982 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2167 5567 6777 2167
Size: 3400 1210 3065
Fragments arranged by size:
3400 3065 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7675
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3956 4178 4785
Size: 107 122 71 631 605 2284 222 607
Cuts at: 4785 6286 136
Size: 1501 1525
Fragments arranged by size:
2284 1525 1501 631 607 605 222 122
107 71
BglII A'GATC_T
Cuts at: 2725 7670 2725
Size: 4945 2730
BlpI GC'TnA_GC
Cuts at: 3375 3618 3740 5092 3375
Size: 243 122 1352 5958
Fragments arranged by size:
5958 1352 243 122
Bme1580I G_kGCm'C
Cuts at: 1790 2417 3088 3225 3342 4030 4369 4723 5736
Size: 627 671 137 117 688 339 354 1013
Cuts at: 5736 6892 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1013 688 671 627 354 339
137 117
BmgBI CAC'GTC
Cuts at: 1318 3694 1318
Size: 2376 5299
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4024 5406 6364
Size: 416 53 83 399 24 2607 1382 958
Cuts at: 6364 442
Size: 1753
Fragments arranged by size:
2607 1753 1382 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2468 1056
Size: 1412 6263
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2870
Size: 7675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 2467 2882 3293 3416 3503 3509 3563
Size: 1226 2 415 411 123 87 6 54
Cuts at: 3563 4178 4500 4637 6335 1239
Size: 615 322 137 1698 2579
Fragments arranged by size:
2579 1698 1226 615 415 411 322 137
123 87 54 6 2
Bpu10I CC'TnA_GC
Cuts at: 3933 4128 3933
Size: 195 7480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3466 3587 5800 6665 6963 3466
Size: 121 2213 865 298 4178
Fragments arranged by size:
4178 2213 865 298 121
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3539 3798 4349 6338 882
Size: 617 2040 259 551 1989 2219
Fragments arranged by size:
2219 2040 1989 617 551 259
BsaAI yAC'GTr
Cuts at: 493 1204 4232 5466 493
Size: 711 3028 1234 2702
Fragments arranged by size:
3028 2702 1234 711
BsaBI GATnn'nnATC
Cuts at: 7669 7669
Size: 7675
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2850 4267 5482 5864
Size: 53 83 186 936 1317 1417 1215 382
Cuts at: 5864 6499 275
Size: 635 1451
Fragments arranged by size:
1451 1417 1317 1215 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6108 6506 6780 6927 7338 6108
Size: 398 274 147 411 6445
Fragments arranged by size:
6445 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 1178
Size: 30 165 30 1035 30 6385
Fragments arranged by size:
6385 1035 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3092 3251 3485 4094 4097 4100 4109 4112
Size: 1906 159 234 609 3 3 9 3
Cuts at: 4112 4115 4118 4121 4124 4226 4229 4577 4610
Size: 3 3 3 3 102 3 348 33
Cuts at: 4610 1186
Size: 4251
Fragments arranged by size:
4251 1906 609 348 234 159 102 33
9 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 3331 3706 3718 3900 4005 4318 4723 6878 7374
Size: 375 12 182 105 313 405 2155 496
Cuts at: 7374 3331
Size: 3632
Fragments arranged by size:
3632 2155 496 405 375 313 182 105
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4830 1163
Size: 573 3094 4008
Fragments arranged by size:
4008 3094 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3501 3562 4654 5125 5886 6914
Size: 275 186 1500 61 1092 471 761 1028
Cuts at: 6914 1540
Size: 2301
Fragments arranged by size:
2301 1500 1092 1028 761 471 275 186
61
BsiHKAI G_wGCw'C
Cuts at: 3225 3381 3507 3603 4413 5736 5821 6892 3225
Size: 156 126 96 810 1323 85 1071 4008
Fragments arranged by size:
4008 1323 1071 810 156 126 96 85
BsmI GAATG_Cn'
Cuts at: 2585 4920 5186 5279 2585
Size: 2335 266 93 4981
Fragments arranged by size:
4981 2335 266 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2841 3269 2841
Size: 428 7247
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2417 2602 2782 3088 3225 3330 3342
Size: 399 627 185 180 306 137 105 12
Cuts at: 3342 3381 3507 3603 4030 4369 4413 4710 4723
Size: 39 126 96 427 339 44 297 13
Cuts at: 4723 4994 5736 5821 6892 1391
Size: 271 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 627 427 399 339 306
297 271 185 180 137 126 105 96
85 44 39 13 12
BspEI T'CCGG_A
Cuts at: 7338 7338
Size: 7675
BspHI T'CATG_A
Cuts at: 2284 5564 7294 2284
Size: 3280 1730 2665
Fragments arranged by size:
3280 2665 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3100 3208 3390 3831 4287 5030 844
Size: 2256 108 182 441 456 743 3489
Fragments arranged by size:
3489 2256 743 456 441 182 108
BsrBI CCG'CTC
Cuts at: 1394 1823 2776 3082 3493 3630 4546 5562 1394
Size: 429 953 306 411 137 916 1016 3507
Fragments arranged by size:
3507 1016 953 916 429 411 306 137
BsrDI GCAATG_nn'
Cuts at: 65 1248 2893 4506 6170 6344 65
Size: 1183 1645 1613 1664 174 1396
Fragments arranged by size:
1664 1645 1613 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2451 3030 4263 4784 6319 6506 1700
Size: 110 641 579 1233 521 1535 187 2869
Fragments arranged by size:
2869 1535 1233 641 579 521 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7675
BssHII G'CGCG_C
Cuts at: 1925 3527 1925
Size: 1602 6073
BssSI C'ACGA_G
Cuts at: 3905 5735 6747 3905
Size: 1830 1012 4833
Fragments arranged by size:
4833 1830 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2426 3199 4255 4858 4917 794
Size: 1632 773 1056 603 59 3552
Fragments arranged by size:
3552 1632 1056 773 603 59
BstBI TT'CG_AA
Cuts at: 6479 6479
Size: 7675
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7675
BstXI CCAn_nnnn'nTGG
Cuts at: 4478 4739 4478
Size: 261 7414
BstYI r'GATC_y
Cuts at: 1972 2440 2640 2725 5005 5372 5757 5774 7226
Size: 468 200 85 2280 367 385 17 1452
Cuts at: 7226 7312 7324 7670 1972
Size: 86 12 346 1977
Fragments arranged by size:
2280 1977 1452 468 385 367 346 200
86 85 17 12
Bsu36I CC'TnA_GG
Cuts at: 2169 2603 2855 4305 2169
Size: 434 252 1450 5539
Fragments arranged by size:
5539 1450 434 252
BtgI C'CryG_G
Cuts at: 513 1065 3072 4173 4380 513
Size: 552 2007 1101 207 3808
Fragments arranged by size:
3808 2007 1101 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4445 487
Size: 683 56 3219 3717
Fragments arranged by size:
3717 3219 683 56
BtsI GCAGTG_nn'
Cuts at: 3674 4539 4795 4842 5191 5985 6005 3674
Size: 865 256 47 349 794 20 5344
Fragments arranged by size:
5344 865 794 349 256 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7675
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7640
DraI TTT'AAA
Cuts at: 4979 4979
Size: 7675
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6682 809
Size: 507 5366 1802
Fragments arranged by size:
5366 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2730 3498
Size: 54 1425 50 275 18 204 696 768
Cuts at: 3498 3837 3948 5122 6011 7640 8
Size: 339 111 1174 889 1629 43
Fragments arranged by size:
1629 1425 1174 889 768 696 339 275
204 111 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3498 5122 1537
Size: 275 1686 1624 4090
Fragments arranged by size:
4090 1686 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2916 3764 5605 1353
Size: 1563 848 1841 3423
Fragments arranged by size:
3423 1841 1563 848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3536 6262 6636 6782 387
Size: 1064 138 1947 2726 374 146 1280
Fragments arranged by size:
2726 1947 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3505 3601 4411 3505
Size: 96 810 6769
Fragments arranged by size:
6769 810 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 2465 2467 2742 2882 2918 3293
Size: 393 824 9 2 275 140 36 375
Cuts at: 3293 3416 3503 3509 3563 3770 3788 4178 4262
Size: 123 87 6 54 207 18 390 84
Cuts at: 4262 4454 4500 4559 4637 4698 5738 6335 7122
Size: 192 46 59 78 61 1040 597 787
Cuts at: 7122 7390 1239
Size: 268 1524
Fragments arranged by size:
1524 1040 824 787 597 393 390 375
275 268 207 192 140 123 87 84
78 61 59 54 46 36 18 9
6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 3107 3386 4056 4803 3107
Size: 279 670 747 5979
Fragments arranged by size:
5979 747 670 279
EcoO109I rG'GnC_Cy
Cuts at: 2413 2414 2893 2935 3338 3339 4153 4366 4719
Size: 1 479 42 403 1 814 213 353
Cuts at: 4719 4720 2413
Size: 1 5368
Fragments arranged by size:
5368 814 479 403 353 213 42 1
1 1
EcoRI G'AATT_C
Cuts at: 4984 5362 4984
Size: 378 7297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2945 2945
Size: 7675
FspI TGC'GCA
Cuts at: 6181 6181
Size: 7675
HaeII r_GCGC'y
Cuts at: 1678 3183 3991 4270 4897 6822 1678
Size: 1505 808 279 627 1925 2531
Fragments arranged by size:
2531 1925 1505 808 627 279
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2079 6316 6348 6390
Size: 32 135 32 475 32 4237 32 42
Cuts at: 6390 6422 1373
Size: 32 2626
Fragments arranged by size:
4237 2626 475 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2835 5019 5265 669
Size: 988 281 897 2184 246 3079
Fragments arranged by size:
3079 2184 988 897 281 246
HindIII A'AGCT_T
Cuts at: 748 2927 748
Size: 2179 5496
HpaI GTT'AAC
Cuts at: 2835 5265 2835
Size: 2430 5245
KasI G'GCGC_C
Cuts at: 4266 4266
Size: 7675
KpnI G_GTAC'C
Cuts at: 2458 5000 2458
Size: 2542 5133
MfeI C'AATT_G
Cuts at: 5274 5274
Size: 7675
MluI A'CGCG_T
Cuts at: 7551 7551
Size: 7675
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2541 2751 3663 6789 6973 1305
Size: 684 552 210 912 3126 184 2007
Fragments arranged by size:
3126 2007 912 684 552 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2732 10
Size: 54 1425 343 900 4953
Fragments arranged by size:
4953 1425 900 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 5633 5992 6151 518
Size: 648 1098 3369 359 159 2042
Fragments arranged by size:
3369 2042 1098 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3091 3613 3853 3916 4196 4240 4685 4864
Size: 1485 522 240 63 280 44 445 179
Cuts at: 4864 5091 5768 6916 7161 1606
Size: 227 677 1148 245 2120
Fragments arranged by size:
2120 1485 1148 677 522 445 280 245
240 227 179 63 44
NaeI GCC'GGC
Cuts at: 4265 4786 4265
Size: 521 7154
NarI GG'CG_CC
Cuts at: 4267 4267
Size: 7675
NcoI C'CATG_G
Cuts at: 513 1065 3072 4173 4380 513
Size: 552 2007 1101 207 3808
Fragments arranged by size:
3808 2007 1101 552 207
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7675
NgoMIV G'CCGG_C
Cuts at: 4263 4784 4263
Size: 521 7154
NheI G'CTAG_C
Cuts at: 1052 2464 1052
Size: 1412 6263
NotI GC'GGCC_GC
Cuts at: 5122 5122
Size: 7675
NspI r_CATG'y
Cuts at: 1132 1229 2400 2696 2962 5033 6578 1132
Size: 97 1171 296 266 2071 1545 2229
Fragments arranged by size:
2229 2071 1545 1171 296 266 97
PasI CC'CwG_GG
Cuts at: 1348 4052 4325 1348
Size: 2704 273 4698
Fragments arranged by size:
4698 2704 273
PciI A'CATG_T
Cuts at: 2396 6574 2396
Size: 4178 3497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3814 3907 1108
Size: 2706 93 4876
Fragments arranged by size:
4876 2706 93
PfoI T'CCnGG_A
Cuts at: 3540 4146 3540
Size: 606 7069
PmeI GTTT'AAAC
Cuts at: 4979 4979
Size: 7675
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5744 5776 7257 7289 5744
Size: 32 1481 32 6130
Fragments arranged by size:
6130 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2893 2935 4153 2893
Size: 42 1218 6415
Fragments arranged by size:
6415 1218 42
PshAI GACnn'nnGTC
Cuts at: 1613 2399 1613
Size: 786 6889
PsiI TTA'TAA
Cuts at: 5245 5245
Size: 7675
PspOMI G'GGCC_C
Cuts at: 2413 3338 4026 4365 4719 2413
Size: 925 688 339 354 5369
Fragments arranged by size:
5369 925 688 354 339
PstI C_TGCA'G
Cuts at: 830 2745 3627 3681 3732 3795 3828 3897 3921
Size: 1915 882 54 51 63 33 69 24
Cuts at: 3921 4374 4395 4626 5027 830
Size: 453 21 231 401 3478
Fragments arranged by size:
3478 1915 882 453 401 231 69 63
54 51 33 24 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7675
PvuII CAG'CTG
Cuts at: 3091 3613 3853 3916 4196 4240 4685 4864 3091
Size: 522 240 63 280 44 445 179 5902
Fragments arranged by size:
5902 522 445 280 240 179 63 44
SacI G_AGCT'C
Cuts at: 3507 3603 4413 3507
Size: 96 810 6769
Fragments arranged by size:
6769 810 96
SalI G'TCGA_C
Cuts at: 1936 5017 1936
Size: 3081 4594
SapI GCTCTTCn'nnn_
Cuts at: 3764 3764
Size: 7675
SbfI CC_TGCA'GG
Cuts at: 4374 5027 4374
Size: 653 7022
ScaI AGT'ACT
Cuts at: 5923 7635 5923
Size: 1712 5963
SfcI C'TryA_G
Cuts at: 826 1045 2091 2741 3623 3677 3728 3791 3824
Size: 219 1046 650 882 54 51 63 33
Cuts at: 3824 3893 3917 4197 4370 4391 4622 5023 6158
Size: 69 24 280 173 21 231 401 1135
Cuts at: 6158 6839 7030 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1046 882 681 650 401 280
231 219 191 173 69 63 54 51
33 24 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 3956 3956
Size: 7675
SfoI GGC'GCC
Cuts at: 4268 4268
Size: 7675
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7675
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7675
SmaI CCC'GGG
Cuts at: 1872 4889 5002 1872
Size: 3017 113 4545
Fragments arranged by size:
4545 3017 113
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3445 3602 5779 6680 6942 820
Size: 197 926 1502 157 2177 901 262 1553
Fragments arranged by size:
2177 1553 1502 926 901 262 197 157
SnaBI TAC'GTA
Cuts at: 493 5466 493
Size: 4973 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7675
SphI G_CATG'C
Cuts at: 1132 2696 2962 5033 1132
Size: 1564 266 2071 3774
Fragments arranged by size:
3774 2071 1564 266
SspI AAT'ATT
Cuts at: 5 52 5599 5
Size: 47 5547 2081
Fragments arranged by size:
5547 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7675
StyI C'CwwG_G
Cuts at: 513 1065 2181 3072 4173 4380 4804 5114 513
Size: 552 1116 891 1101 207 424 310 3074
Fragments arranged by size:
3074 1116 1101 891 552 424 310 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 2894 4019 5702 5719 5872 6057
Size: 1255 614 700 1125 1683 17 153 185
Cuts at: 6057 6502 325
Size: 445 1498
Fragments arranged by size:
1683 1498 1255 1125 700 614 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4503 4566 5921
Size: 274 80 33 51 1443 2526 63 1355
Cuts at: 5921 7633 96
Size: 1712 138
Fragments arranged by size:
2526 1712 1443 1355 274 138 80 63
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4321 4353 7635 7667 4321
Size: 32 3282 32 4329
Fragments arranged by size:
4329 3282 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2297 1316
Size: 981 6694
XbaI T'CTAG_A
Cuts at: 5011 5011
Size: 7675
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2369 2369
Size: 7675
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7675
XmaI C'CCGG_G
Cuts at: 1870 4887 5000 1870
Size: 3017 113 4545
Fragments arranged by size:
4545 3017 113
XmnI GAAnn'nnTTC
Cuts at: 5804 5804
Size: 7675
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5483 276
Size: 53 83 186 936 3949 2468
Fragments arranged by size:
3949 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI