Restriction map for
pFN21AB8544
(Circular) (Six-base) MAPSORT of: pFN21AB8544.seq Check: 2172 from: 1 to: 7411
pFN21AB8544 7411 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4663 5221 278
Size: 53 83 186 936 3127 558 2468
Fragments arranged by size:
3127 2468 936 558 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4283 4754 1937
Size: 2346 471 4594
Fragments arranged by size:
4594 2346 471
Acc65I G'GTAC_C
Cuts at: 4732 4732
Size: 7411
AclI AA'CG_TT
Cuts at: 1563 5538 5911 1563
Size: 3975 373 3063
Fragments arranged by size:
3975 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2931 3162 3201 3671 3875 5474 6858
Size: 678 621 231 39 470 204 1599 1384
Cuts at: 6858 7126 1632
Size: 268 1917
Fragments arranged by size:
1917 1599 1384 678 621 470 268 231
204 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7214
AflIII A'CryG_T
Cuts at: 1203 4452 4454 6310 7287 1203
Size: 3249 2 1856 977 1327
Fragments arranged by size:
3249 1856 1327 977 2
AgeI A'CCGG_T
Cuts at: 6242 6242
Size: 7411
AhdI GACnn_n'nnGTC
Cuts at: 6140 6140
Size: 7411
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7411
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7411
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2781 2813 2781
Size: 32 7379
AlwNI CAG_nnn'CTG
Cuts at: 2288 5154 6726 2288
Size: 2866 1572 2973
Fragments arranged by size:
2973 2866 1572
ApaLI G'TGCA_C
Cuts at: 5468 6624 5468
Size: 1156 6255
ApoI r'AATT_y
Cuts at: 1493 2177 2667 3548 3759 4393 4470 4567 4720
Size: 684 490 881 211 634 77 97 153
Cuts at: 4720 4946 5098 7207 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 881 684 634 490 226 211
153 152 97 77
AseI AT'TA_AT
Cuts at: 160 1032 5965 160
Size: 872 4933 1606
Fragments arranged by size:
4933 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2973 4736 1870
Size: 73 71 959 1763 4545
Fragments arranged by size:
4545 1763 959 73 71
AvrII C'CTAG_G
Cuts at: 3250 3250
Size: 7411
BamHI G'GATC_C
Cuts at: 4741 5108 4741
Size: 367 7044
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4732 6187 618
Size: 325 217 627 2945 1455 1842
Fragments arranged by size:
2945 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2834 2850 3312 3663 4730 1391
Size: 1443 16 462 351 1067 4072
Fragments arranged by size:
4072 1443 1067 462 351 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2481 2622 3587 4712 928
Size: 971 582 141 965 1125 3627
Fragments arranged by size:
3627 1125 971 965 582 141
BbvCI CC'TCA_GC
Cuts at: 2201 2201
Size: 7411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5602 5636 1903
Size: 34 3665 34 3678
Fragments arranged by size:
3678 3665 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3958 5303 6513 3958
Size: 1345 1210 4856
Fragments arranged by size:
4856 1345 1210
BclI T'GATC_A
Cuts at: 1548 2437 1548
Size: 889 6522
BfrBI ATG'CAT
Cuts at: 3678 3678
Size: 7411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6022 136
Size: 107 122 71 631 605 4350 1525
Fragments arranged by size:
4350 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4630 7406 4630
Size: 2776 4635
BlpI GC'TnA_GC
Cuts at: 2457 4828 2457
Size: 2371 5040
Bme1580I G_kGCm'C
Cuts at: 1790 3154 4452 5472 6628 1790
Size: 1364 1298 1020 1156 2573
Fragments arranged by size:
2573 1364 1298 1156 1020
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5142 6100 442
Size: 416 53 83 399 24 3725 958 1753
Fragments arranged by size:
3725 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 3693 6071 1239
Size: 1163 1291 2378 2579
Fragments arranged by size:
2579 2378 1291 1163
Bpu10I CC'TnA_GC
Cuts at: 2201 2201
Size: 7411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2802 2940 3589 4510 5536 6401 6699 2802
Size: 138 649 921 1026 865 298 3514
Fragments arranged by size:
3514 1026 921 865 649 298 138
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6074 882
Size: 617 4575 2219
Fragments arranged by size:
4575 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3776 4455 5202 493
Size: 711 2572 679 747 2702
Fragments arranged by size:
2702 2572 747 711 679
BsaBI GATnn'nnATC
Cuts at: 3136 3197 4635 7405 3136
Size: 61 1438 2770 3142
Fragments arranged by size:
3142 2770 1438 61
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4660 5218 5600 6235
Size: 53 83 186 936 3127 558 382 635
Cuts at: 6235 275
Size: 1451
Fragments arranged by size:
3127 1451 936 635 558 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5844 6242 6516 6663 7074 5844
Size: 398 274 147 411 6181
Fragments arranged by size:
6181 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3444 3474 4050 4080 4235
Size: 30 165 30 2041 30 576 30 155
Cuts at: 4235 4265 4295 1178
Size: 30 30 4294
Fragments arranged by size:
4294 2041 576 165 155 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2940 2943 4071 4286 4442 1186
Size: 1754 3 1128 215 156 4155
Fragments arranged by size:
4155 1754 1128 215 156 3
BseYI C'CCAG_C
Cuts at: 6614 7110 6614
Size: 496 6915
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2301 3616 1163
Size: 573 565 1315 4958
Fragments arranged by size:
4958 1315 573 565
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4861 5622 6650 1540
Size: 275 186 2860 761 1028 2301
Fragments arranged by size:
2860 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2592 2850 5472 5557 6628 2592
Size: 258 2622 85 1071 3375
Fragments arranged by size:
3375 2622 1071 258 85
BsmI GAATG_Cn'
Cuts at: 3680 4922 5015 3680
Size: 1242 93 6076
Fragments arranged by size:
6076 1242 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2592 2834 2850 3154 3312 3663 4452
Size: 399 802 242 16 304 158 351 789
Cuts at: 4452 4730 5472 5557 6628 1391
Size: 278 742 85 1071 2174
Fragments arranged by size:
2174 1071 802 789 742 399 351 304
278 242 158 85 16
BspEI T'CCGG_A
Cuts at: 7074 7074
Size: 7411
BspHI T'CATG_A
Cuts at: 2095 2224 3829 5300 7030 2095
Size: 129 1605 1471 1730 2476
Fragments arranged by size:
2476 1730 1605 1471 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4766 844
Size: 3922 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5298 1394
Size: 429 3475 3507
Fragments arranged by size:
3507 3475 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5906 6080 65
Size: 1183 4658 174 1396
Fragments arranged by size:
4658 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2526 6055 6242 1700
Size: 110 716 3529 187 2869
Fragments arranged by size:
3529 2869 716 187 110
BsrGI T'GTAC_A
Cuts at: 96 4079 96
Size: 3983 3428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7411
BssSI C'ACGA_G
Cuts at: 5471 6483 5471
Size: 1012 6399
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7411
BstBI TT'CG_AA
Cuts at: 6215 6215
Size: 7411
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7411
BstYI r'GATC_y
Cuts at: 1972 3478 3976 4630 4741 5108 5493 5510 6962
Size: 1506 498 654 111 367 385 17 1452
Cuts at: 6962 7048 7060 7406 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1506 1452 654 498 385 367 346
111 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3862 513
Size: 552 2797 4062
Fragments arranged by size:
4062 2797 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6672
Fragments arranged by size:
6672 683 56
BtsI GCAGTG_nn'
Cuts at: 4927 5721 5741 4927
Size: 794 20 6597
Fragments arranged by size:
6597 794 20
ClaI AT'CG_AT
Cuts at: 1554 3490 1554
Size: 1936 5475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7376
DraI TTT'AAA
Cuts at: 3757 4363 4715 3757
Size: 606 352 6453
Fragments arranged by size:
6453 606 352
DraIII CAC_nnn'GTG
Cuts at: 2596 2596
Size: 7411
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6418 809
Size: 507 5102 1802
Fragments arranged by size:
5102 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2709 4858 5747
Size: 54 1425 50 275 18 879 2149 889
Cuts at: 5747 7376 8
Size: 1629 43
Fragments arranged by size:
2149 1629 1425 889 879 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4858 1537
Size: 275 3046 4090
Fragments arranged by size:
4090 3046 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4215 4263 5341 1353
Size: 2862 48 1078 3423
Fragments arranged by size:
3423 2862 1078 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5998 6372 6518 387
Size: 1064 138 4409 374 146 1280
Fragments arranged by size:
4409 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2848 2848
Size: 7411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2402 2931 3162 3201 3671 3693
Size: 393 678 92 529 231 39 470 22
Cuts at: 3693 3875 5474 6071 6858 7126 1239
Size: 182 1599 597 787 268 1524
Fragments arranged by size:
1599 1524 787 678 597 529 470 393
268 231 182 92 39 22
EcoO109I rG'GnC_Cy
Cuts at: 2068 2822 2068
Size: 754 6657
EcoRI G'AATT_C
Cuts at: 2177 4720 5098 2177
Size: 2543 378 4490
Fragments arranged by size:
4490 2543 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3552 3581 3552
Size: 29 7382
FspI TGC'GCA
Cuts at: 5917 5917
Size: 7411
HaeII r_GCGC'y
Cuts at: 1678 6558 1678
Size: 4880 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2735 2767 2938 2970 3961
Size: 32 135 32 1163 32 171 32 991
Cuts at: 3961 3993 4214 4235 4246 4265 4267 4297 6052
Size: 32 221 21 11 19 2 30 1755
Cuts at: 6052 6084 6126 6158 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1755 1163 991 221 171 135 42
32 32 32 32 32 32 32 30
21 19 11 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2524 4755 5001 669
Size: 988 281 586 2231 246 3079
Fragments arranged by size:
3079 2231 988 586 281 246
HindIII A'AGCT_T
Cuts at: 748 2402 3390 748
Size: 1654 988 4769
Fragments arranged by size:
4769 1654 988
HpaI GTT'AAC
Cuts at: 2524 5001 2524
Size: 2477 4934
KpnI G_GTAC'C
Cuts at: 4736 4736
Size: 7411
MfeI C'AATT_G
Cuts at: 5010 5010
Size: 7411
MluI A'CGCG_T
Cuts at: 7287 7287
Size: 7411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6525 6709 1305
Size: 5220 184 2007
Fragments arranged by size:
5220 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2711 10
Size: 54 1425 343 879 4710
Fragments arranged by size:
4710 1425 879 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3673 4308 5369 5728 5887 518
Size: 648 2507 635 1061 359 159 2042
Fragments arranged by size:
2507 2042 1061 648 635 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2200 2456 4827 5504 6652 6897 1606
Size: 419 175 256 2371 677 1148 245 2120
Fragments arranged by size:
2371 2120 1148 677 419 256 245 175
NcoI C'CATG_G
Cuts at: 513 1065 3862 513
Size: 552 2797 4062
Fragments arranged by size:
4062 2797 552
NdeI CA'TA_TG
Cuts at: 387 2298 387
Size: 1911 5500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7411
NotI GC'GGCC_GC
Cuts at: 4858 4858
Size: 7411
NsiI A_TGCA'T
Cuts at: 3680 3680
Size: 7411
NspI r_CATG'y
Cuts at: 1132 1229 4769 6314 1132
Size: 97 3540 1545 2229
Fragments arranged by size:
3540 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3653 1348
Size: 2305 5106
PciI A'CATG_T
Cuts at: 6310 6310
Size: 7411
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2860 1108
Size: 1752 5659
PfoI T'CCnGG_A
Cuts at: 3979 3979
Size: 7411
PmeI GTTT'AAAC
Cuts at: 4715 4715
Size: 7411
PmlI CAC'GTG
Cuts at: 3776 4455 3776
Size: 679 6732
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3808 3840 5480 5512 6993 7025 3808
Size: 32 1640 32 1481 32 4194
Fragments arranged by size:
4194 1640 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2822 2068
Size: 754 6657
PshAI GACnn'nnGTC
Cuts at: 1613 2694 1613
Size: 1081 6330
PsiI TTA'TAA
Cuts at: 3227 4981 3227
Size: 1754 5657
PspXI vC'TCGA_Gb
Cuts at: 2973 2973
Size: 7411
PstI C_TGCA'G
Cuts at: 830 2047 4763 830
Size: 1217 2716 3478
Fragments arranged by size:
3478 2716 1217
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7411
PvuII CAG'CTG
Cuts at: 2025 2200 2456 2025
Size: 175 256 6980
Fragments arranged by size:
6980 256 175
SacI G_AGCT'C
Cuts at: 2850 2850
Size: 7411
SalI G'TCGA_C
Cuts at: 1936 4753 1936
Size: 2817 4594
SbfI CC_TGCA'GG
Cuts at: 4763 4763
Size: 7411
ScaI AGT'ACT
Cuts at: 5659 7371 5659
Size: 1712 5699
SexAI A'CCwGG_T
Cuts at: 3851 3851
Size: 7411
SfcI C'TryA_G
Cuts at: 826 1045 2043 2384 2766 4759 5894 6575 6766
Size: 219 998 341 382 1993 1135 681 191
Cuts at: 6766 826
Size: 1471
Fragments arranged by size:
1993 1471 1135 998 681 382 341 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7411
SmaI CCC'GGG
Cuts at: 1872 4738 1872
Size: 2866 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2817 2955 2973 3604 4525 5515
Size: 197 926 874 138 18 631 921 990
Cuts at: 5515 6416 6678 820
Size: 901 262 1553
Fragments arranged by size:
1553 990 926 921 901 874 631 262
197 138 18
SnaBI TAC'GTA
Cuts at: 493 5202 493
Size: 4709 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7411
SphI G_CATG'C
Cuts at: 1132 4769 1132
Size: 3637 3774
SspI AAT'ATT
Cuts at: 5 52 4680 5335 5
Size: 47 4628 655 2081
Fragments arranged by size:
4628 2081 655 47
StuI AGG'CCT
Cuts at: 1517 3617 1517
Size: 2100 5311
StyI C'CwwG_G
Cuts at: 513 1065 2836 3250 3523 3862 4850 513
Size: 552 1771 414 273 339 988 3074
Fragments arranged by size:
3074 1771 988 552 414 339 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4567 5438 5455 5608 5793 6238 325
Size: 1255 2987 871 17 153 185 445 1498
Fragments arranged by size:
2987 1498 1255 871 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2107 4079 5657
Size: 274 80 33 51 1443 130 1972 1578
Cuts at: 5657 7369 96
Size: 1712 138
Fragments arranged by size:
1972 1712 1578 1443 274 138 130 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3444 3476 4050 4082 7371 7403 3444
Size: 32 574 32 3289 32 3452
Fragments arranged by size:
3452 3289 574 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4328 1316
Size: 3012 4399
XbaI T'CTAG_A
Cuts at: 3113 4747 3113
Size: 1634 5777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4569 4569
Size: 7411
XhoI C'TCGA_G
Cuts at: 1943 2973 1943
Size: 1030 6381
XmaI C'CCGG_G
Cuts at: 1870 4736 1870
Size: 2866 4545
XmnI GAAnn'nnTTC
Cuts at: 2620 4098 5540 2620
Size: 1478 1442 4491
Fragments arranged by size:
4491 1478 1442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4661 5219 276
Size: 53 83 186 936 3127 558 2468
Fragments arranged by size:
3127 2468 936 558 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI