Restriction map for
pFN21AB8541
(Circular) (Six-base) MAPSORT of: pFN21AB8541.seq Check: 5018 from: 1 to: 7642
pFN21AB8541 7642 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2574 2818 3262 3749 4119 2574
Size: 244 444 487 370 6097
Fragments arranged by size:
6097 487 444 370 244
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5452 278
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4985 1937
Size: 3048 4594
Acc65I G'GTAC_C
Cuts at: 4963 4963
Size: 7642
AclI AA'CG_TT
Cuts at: 1563 5769 6142 1563
Size: 4206 373 3063
Fragments arranged by size:
4206 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2719 2739 4283 4592 5705 7089 7357 1632
Size: 1087 20 1544 309 1113 1384 268 1917
Fragments arranged by size:
1917 1544 1384 1113 1087 309 268 20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7445
AflIII A'CryG_T
Cuts at: 1203 3498 6541 7518 1203
Size: 2295 3043 977 1327
Fragments arranged by size:
3043 2295 1327 977
AgeI A'CCGG_T
Cuts at: 6473 6473
Size: 7642
AhdI GACnn_n'nnGTC
Cuts at: 6371 6371
Size: 7642
AleI CACnn'nnGTG
Cuts at: 1166 3746 4731 1166
Size: 2580 985 4077
Fragments arranged by size:
4077 2580 985
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3015 1184
Size: 1831 5811
AlwNI CAG_nnn'CTG
Cuts at: 2142 3016 3103 3577 3895 5385 6957 2142
Size: 874 87 474 318 1490 1572 2827
Fragments arranged by size:
2827 1572 1490 874 474 318 87
ApaI G_GGCC'C
Cuts at: 3865 4361 3865
Size: 496 7146
ApaLI G'TGCA_C
Cuts at: 3494 4724 5699 6855 3494
Size: 1230 975 1156 4281
Fragments arranged by size:
4281 1230 1156 975
ApoI r'AATT_y
Cuts at: 1493 4951 5177 5329 7438 1493
Size: 3458 226 152 2109 1697
Fragments arranged by size:
3458 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6196 160
Size: 872 5164 1606
Fragments arranged by size:
5164 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2089 4967 1870
Size: 73 90 56 2878 4545
Fragments arranged by size:
4545 2878 90 73 56
BamHI G'GATC_C
Cuts at: 2039 4972 5339 2039
Size: 2933 367 4342
Fragments arranged by size:
4342 2933 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2010 2392 2881 3274 4487
Size: 325 217 627 223 382 489 393 1213
Cuts at: 4487 4754 4963 6418 618
Size: 267 209 1455 1842
Fragments arranged by size:
1842 1455 1213 627 489 393 382 325
267 223 217 209
BanII G_rGCy'C
Cuts at: 1391 2367 2681 3645 3865 4361 4961 1391
Size: 976 314 964 220 496 600 4072
Fragments arranged by size:
4072 976 964 600 496 314 220
BbeI G_GCGC'C
Cuts at: 2014 2014
Size: 7642
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6671
BbvCI CC'TCA_GC
Cuts at: 3391 4622 3391
Size: 1231 6411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4310 4344 4402 4436 5833
Size: 34 50 34 2289 34 58 34 1397
Cuts at: 5833 5867 1903
Size: 34 3678
Fragments arranged by size:
3678 2289 1397 58 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5534 6744 5534
Size: 1210 6432
BclI T'GATC_A
Cuts at: 1548 3433 1548
Size: 1885 5757
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2094 3357 6253
Size: 107 122 71 631 605 422 1263 2896
Cuts at: 6253 136
Size: 1525
Fragments arranged by size:
2896 1525 1263 631 605 422 122 107
71
BglII A'GATC_T
Cuts at: 7637 7637
Size: 7642
BlpI GC'TnA_GC
Cuts at: 2078 2538 3486 5059 2078
Size: 460 948 1573 4661
Fragments arranged by size:
4661 1573 948 460
Bme1580I G_kGCm'C
Cuts at: 1790 2116 3498 3865 4361 4643 4728 5703 6859
Size: 326 1382 367 496 282 85 975 1156
Cuts at: 6859 1790
Size: 2573
Fragments arranged by size:
2573 1382 1156 975 496 367 326 282
85
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2231 2305 3365
Size: 416 53 83 399 24 814 74 1060
Cuts at: 3365 3976 5373 6331 442
Size: 611 1397 958 1753
Fragments arranged by size:
1753 1397 1060 958 814 611 416 399
83 74 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3091 4507 3091
Size: 1416 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2436 2647 3241 3494 4850 6302 1239
Size: 1197 211 594 253 1356 1452 2579
Fragments arranged by size:
2579 1452 1356 1197 594 253 211
Bpu10I CC'TnA_GC
Cuts at: 3066 3391 3446 4622 3066
Size: 325 55 1176 6086
Fragments arranged by size:
6086 1176 325 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4910 5767 6632 6930 4910
Size: 857 865 298 5622
Fragments arranged by size:
5622 865 857 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 2054 2395 2674 6305 882
Size: 617 537 18 341 279 3631 2219
Fragments arranged by size:
3631 2219 617 537 341 279 18
BsaAI yAC'GTr
Cuts at: 493 1204 4504 5433 493
Size: 711 3300 929 2702
Fragments arranged by size:
3300 2702 929 711
BsaBI GATnn'nnATC
Cuts at: 4814 7636 4814
Size: 2822 4820
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2011 5449 5831 6466
Size: 53 83 186 936 478 3438 382 635
Cuts at: 6466 275
Size: 1451
Fragments arranged by size:
3438 1451 936 635 478 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3949 6075 6473 6747 6894 7305 3949
Size: 2126 398 274 147 411 4286
Fragments arranged by size:
4286 2126 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3123 3153 1178
Size: 30 165 30 1720 30 5667
Fragments arranged by size:
5667 1720 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2506 3280 3692 4058 1186
Size: 865 455 774 412 366 4770
Fragments arranged by size:
4770 865 774 455 412 366
BseYI C'CCAG_C
Cuts at: 2463 2504 2814 2867 2983 3400 3767 4010 6845
Size: 41 310 53 116 417 367 243 2835
Cuts at: 6845 7341 2463
Size: 496 2764
Fragments arranged by size:
2835 2764 496 417 367 310 243 116
53 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3011 3135 4396 4601 1163
Size: 573 1275 124 1261 205 4204
Fragments arranged by size:
4204 1275 1261 573 205 124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5092 5853 6881 1540
Size: 275 186 3091 761 1028 2301
Fragments arranged by size:
3091 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 2565 3498 4533 4728 5703 5788 6859 2265
Size: 300 933 1035 195 975 85 1071 3048
Fragments arranged by size:
3048 1071 1035 975 933 300 195 85
BsmI GAATG_Cn'
Cuts at: 4921 5153 5246 4921
Size: 232 93 7317
Fragments arranged by size:
7317 232 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4586 4586
Size: 7642
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2265 2367 2565 2681 3498 3645
Size: 399 326 149 102 198 116 817 147
Cuts at: 3645 3865 4361 4533 4643 4728 4961 5703 5788
Size: 220 496 172 110 85 233 742 85
Cuts at: 5788 6859 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 817 742 496 399 326 233
220 198 172 149 147 116 110 102
85 85
BspEI T'CCGG_A
Cuts at: 7305 7305
Size: 7642
BspHI T'CATG_A
Cuts at: 5531 7261 5531
Size: 1730 5912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2574 2620 2818 3262 3749 4119 4997 844
Size: 1730 46 198 444 487 370 878 3489
Fragments arranged by size:
3489 1730 878 487 444 370 198 46
BsrBI CCG'CTC
Cuts at: 1394 1823 4407 4707 4866 5529 1394
Size: 429 2584 300 159 663 3507
Fragments arranged by size:
3507 2584 663 429 300 159
BsrDI GCAATG_nn'
Cuts at: 65 1248 6137 6311 65
Size: 1183 4889 174 1396
Fragments arranged by size:
4889 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3178 4048 4218 4630 4645 4751 6286
Size: 110 1368 870 170 412 15 106 1535
Cuts at: 6286 6473 1700
Size: 187 2869
Fragments arranged by size:
2869 1535 1368 870 412 187 170 110
106 15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7642
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7642
BssSI C'ACGA_G
Cuts at: 5702 6714 5702
Size: 1012 6630
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2142 2569 3103 794
Size: 1348 427 534 5333
Fragments arranged by size:
5333 1348 534 427
BstBI TT'CG_AA
Cuts at: 6446 6446
Size: 7642
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7642
BstXI CCAn_nnnn'nTGG
Cuts at: 2645 4853 2645
Size: 2208 5434
BstYI r'GATC_y
Cuts at: 1972 2039 3477 4972 5339 5724 5741 7193 7279
Size: 67 1438 1495 367 385 17 1452 86
Cuts at: 7279 7291 7637 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1495 1452 1438 385 367 346 86
67 17 12
Bsu36I CC'TnA_GG
Cuts at: 3505 3855 3505
Size: 350 7292
BtgI C'CryG_G
Cuts at: 513 1065 2015 2116 3372 3865 4074 4899 513
Size: 552 950 101 1256 493 209 825 3256
Fragments arranged by size:
3256 1256 950 825 552 493 209 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4360 4801 487
Size: 683 56 3134 441 3328
Fragments arranged by size:
3328 3134 683 441 56
BtsI GCAGTG_nn'
Cuts at: 2192 3240 3976 4466 4726 4738 5158 5952 5972
Size: 1048 736 490 260 12 420 794 20
Cuts at: 5972 2192
Size: 3862
Fragments arranged by size:
3862 1048 794 736 490 420 260 20
12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7607
DraI TTT'AAA
Cuts at: 4946 4946
Size: 7642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6649 809
Size: 507 5333 1802
Fragments arranged by size:
5333 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2776 3018 3912
Size: 54 1425 50 275 18 946 242 894
Cuts at: 3912 4475 4628 5089 5978 7607 8
Size: 563 153 461 889 1629 43
Fragments arranged by size:
1629 1425 946 894 889 563 461 275
242 153 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5089 1537
Size: 275 3277 4090
Fragments arranged by size:
4090 3277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2738 5572 1353
Size: 1385 2834 3423
Fragments arranged by size:
3423 2834 1385
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2440 4919 6229 6603 6749 387
Size: 1064 138 851 2479 1310 374 146 1280
Fragments arranged by size:
2479 1310 1280 1064 851 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2436 2647 2719 2739 3241 3494 4283
Size: 393 804 211 72 20 502 253 789
Cuts at: 4283 4592 4850 5705 6302 7089 7357 1239
Size: 309 258 855 597 787 268 1524
Fragments arranged by size:
1524 855 804 789 787 597 502 393
309 268 258 253 211 72 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2711 4133 4937 2711
Size: 1422 804 5416
Fragments arranged by size:
5416 1422 804
EcoO109I rG'GnC_Cy
Cuts at: 2791 2801 3851 3861 3892 4147 4357 4930 2791
Size: 10 1050 10 31 255 210 573 5503
Fragments arranged by size:
5503 1050 573 255 210 31 10 10
EcoRI G'AATT_C
Cuts at: 4951 5329 4951
Size: 378 7264
EcoRV GAT'ATC
Cuts at: 3157 3157
Size: 7642
FspI TGC'GCA
Cuts at: 3708 6148 3708
Size: 2440 5202
HaeII r_GCGC'y
Cuts at: 1678 2014 2106 3838 6789 1678
Size: 336 92 1732 2951 2531
Fragments arranged by size:
2951 2531 1732 336 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2392 2424 3141 3173 3528
Size: 32 135 32 820 32 717 32 355
Cuts at: 3528 3560 6283 6315 6357 6389 1373
Size: 32 2723 32 42 32 2626
Fragments arranged by size:
2723 2626 820 717 355 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4986 5232 669
Size: 988 281 3048 246 3079
Fragments arranged by size:
3079 3048 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7642
HpaI GTT'AAC
Cuts at: 5232 5232
Size: 7642
KasI G'GCGC_C
Cuts at: 2010 2010
Size: 7642
KpnI G_GTAC'C
Cuts at: 4967 4967
Size: 7642
MfeI C'AATT_G
Cuts at: 5241 5241
Size: 7642
MluI A'CGCG_T
Cuts at: 7518 7518
Size: 7642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2269 2290 3127 4506 6756 6940 1305
Size: 964 21 837 1379 2250 184 2007
Fragments arranged by size:
2250 2007 1379 964 837 184 21
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2778 3914 4477 10
Size: 54 1425 343 946 1136 563 3175
Fragments arranged by size:
3175 1425 1136 946 563 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3527 3746 4424 4506 4721 4731 4772
Size: 648 2361 219 678 82 215 10 41
Cuts at: 4772 4839 5600 5959 6118 518
Size: 67 761 359 159 2042
Fragments arranged by size:
2361 2042 761 678 648 359 219 215
159 82 67 41 10
MspA1I CmG'CkG
Cuts at: 1606 2017 2987 3023 3058 3686 4363 4438 4626
Size: 411 970 36 35 628 677 75 188
Cuts at: 4626 4849 5058 5735 6883 7128 1606
Size: 223 209 677 1148 245 2120
Fragments arranged by size:
2120 1148 970 677 677 628 411 245
223 209 188 75 36 35
NaeI GCC'GGC
Cuts at: 4050 4632 4050
Size: 582 7060
NarI GG'CG_CC
Cuts at: 2011 2011
Size: 7642
NcoI C'CATG_G
Cuts at: 513 1065 3865 513
Size: 552 2800 4290
Fragments arranged by size:
4290 2800 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7642
NgoMIV G'CCGG_C
Cuts at: 4048 4630 4048
Size: 582 7060
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7642
NotI GC'GGCC_GC
Cuts at: 5089 5089
Size: 7642
NspI r_CATG'y
Cuts at: 1132 1229 3494 3502 3815 4838 5000 6545 1132
Size: 97 2265 8 313 1023 162 1545 2229
Fragments arranged by size:
2265 2229 1545 1023 313 162 97 8
PasI CC'CwG_GG
Cuts at: 1348 2684 3847 1348
Size: 1336 1163 5143
Fragments arranged by size:
5143 1336 1163
PciI A'CATG_T
Cuts at: 3498 6541 3498
Size: 3043 4599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3910 4140 1108
Size: 2802 230 4610
Fragments arranged by size:
4610 2802 230
PfoI T'CCnGG_A
Cuts at: 3127 3127
Size: 7642
PmeI GTTT'AAAC
Cuts at: 4946 4946
Size: 7642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 7224 7256 5711
Size: 32 1481 32 6097
Fragments arranged by size:
6097 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2791 3892 2791
Size: 1101 6541
PshAI GACnn'nnGTC
Cuts at: 1613 3329 1613
Size: 1716 5926
PsiI TTA'TAA
Cuts at: 5212 5212
Size: 7642
PspOMI G'GGCC_C
Cuts at: 3861 4357 3861
Size: 496 7146
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4679 4711 4679
Size: 32 7610
PstI C_TGCA'G
Cuts at: 830 2102 2535 2625 3012 3099 3460 3691 4733
Size: 1272 433 90 387 87 361 231 1042
Cuts at: 4733 4994 830
Size: 261 3478
Fragments arranged by size:
3478 1272 1042 433 387 361 261 231
90 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7642
PvuII CAG'CTG
Cuts at: 2987 3058 3686 4626 2987
Size: 71 628 940 6003
Fragments arranged by size:
6003 940 628 71
SacII CC_GC'GG
Cuts at: 2018 2018
Size: 7642
SalI G'TCGA_C
Cuts at: 1936 4984 1936
Size: 3048 4594
SbfI CC_TGCA'GG
Cuts at: 4994 4994
Size: 7642
ScaI AGT'ACT
Cuts at: 5890 7602 5890
Size: 1712 5930
SexAI A'CCwGG_T
Cuts at: 2404 4518 2404
Size: 2114 5528
SfcI C'TryA_G
Cuts at: 826 1045 2098 2531 2621 3008 3095 3456 3687
Size: 219 1053 433 90 387 87 361 231
Cuts at: 3687 4729 4990 6125 6806 6997 826
Size: 1042 261 1135 681 191 1471
Fragments arranged by size:
1471 1135 1053 1042 681 433 387 361
261 231 219 191 90 87
SfoI GGC'GCC
Cuts at: 2012 2012
Size: 7642
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7642
SmaI CCC'GGG
Cuts at: 1872 2091 4969 1872
Size: 219 2878 4545
Fragments arranged by size:
4545 2878 219
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4925 5746 6647 6909 820
Size: 197 926 2982 821 901 262 1553
Fragments arranged by size:
2982 1553 926 901 821 262 197
SnaBI TAC'GTA
Cuts at: 493 5433 493
Size: 4940 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7642
SphI G_CATG'C
Cuts at: 1132 3815 5000 1132
Size: 2683 1185 3774
Fragments arranged by size:
3774 2683 1185
SspI AAT'ATT
Cuts at: 5 52 5566 5
Size: 47 5514 2081
Fragments arranged by size:
5514 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7642
StyI C'CwwG_G
Cuts at: 513 1065 2281 2372 2712 2751 2885 3108 3865
Size: 552 1216 91 340 39 134 223 757
Cuts at: 3865 4134 5081 513
Size: 269 947 3074
Fragments arranged by size:
3074 1216 947 757 552 340 269 223
134 91 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2324 2410 2881 3736 5669 5686 5839
Size: 1255 744 86 471 855 1933 17 153
Cuts at: 5839 6024 6469 325
Size: 185 445 1498
Fragments arranged by size:
1933 1498 1255 855 744 471 445 185
153 86 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5888 7600 96
Size: 274 80 33 51 1443 3911 1712 138
Fragments arranged by size:
3911 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2680 2712 7602 7634 2680
Size: 32 4890 32 2688
Fragments arranged by size:
4890 2688 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2406 4775 1316
Size: 1090 2369 4183
Fragments arranged by size:
4183 2369 1090
XbaI T'CTAG_A
Cuts at: 4978 4978
Size: 7642
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7642
XmaI C'CCGG_G
Cuts at: 1870 2089 4967 1870
Size: 219 2878 4545
Fragments arranged by size:
4545 2878 219
XmnI GAAnn'nnTTC
Cuts at: 5771 5771
Size: 7642
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5450 276
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI
PmlI PspXI RsrII SacI SanDI SapI SfiI SrfI
SwaI XcmI