Restriction map for
pFN21AB8539
(Circular) (Six-base) MAPSORT of: pFN21AB8539.seq Check: 1905 from: 1 to: 7570
pFN21AB8539 7570 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2595 4372 4390 4399 4741 2595
Size: 1777 18 9 342 5424
Fragments arranged by size:
5424 1777 342 18 9
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3153 3429 3786 4059
Size: 53 83 186 936 1617 276 357 273
Cuts at: 4059 5380 278
Size: 1321 2468
Fragments arranged by size:
2468 1617 1321 936 357 276 273 186
83 53
AccI GT'mk_AC
Cuts at: 1937 3959 4913 1937
Size: 2022 954 4594
Fragments arranged by size:
4594 2022 954
Acc65I G'GTAC_C
Cuts at: 3237 3390 4891 3237
Size: 153 1501 5916
Fragments arranged by size:
5916 1501 153
AclI AA'CG_TT
Cuts at: 1563 5697 6070 1563
Size: 4134 373 3063
Fragments arranged by size:
4134 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3302 4523 5633 7017 7285 1632
Size: 1670 1221 1110 1384 268 1917
Fragments arranged by size:
1917 1670 1384 1221 1110 268
AfeI AGC'GCT
Cuts at: 4571 4571
Size: 7570
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7373
AflIII A'CryG_T
Cuts at: 1203 6469 7446 1203
Size: 5266 977 1327
Fragments arranged by size:
5266 1327 977
AgeI A'CCGG_T
Cuts at: 6401 6401
Size: 7570
AhdI GACnn_n'nnGTC
Cuts at: 6299 6299
Size: 7570
AleI CACnn'nnGTG
Cuts at: 1166 2607 4845 1166
Size: 1441 2238 3891
Fragments arranged by size:
3891 2238 1441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7570
AlwNI CAG_nnn'CTG
Cuts at: 3016 4127 4219 4255 5313 6885 3016
Size: 1111 92 36 1058 1572 3701
Fragments arranged by size:
3701 1572 1111 1058 92 36
ApaI G_GGCC'C
Cuts at: 4095 4095
Size: 7570
ApaLI G'TGCA_C
Cuts at: 3064 5627 6783 3064
Size: 2563 1156 3851
Fragments arranged by size:
3851 2563 1156
ApoI r'AATT_y
Cuts at: 1493 4306 4879 5105 5257 7366 1493
Size: 2813 573 226 152 2109 1697
Fragments arranged by size:
2813 2109 1697 573 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6124 160
Size: 872 5092 1606
Fragments arranged by size:
5092 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
AvaI C'yCGr_G
Cuts at: 1870 1943 3662 3821 4100 4330 4895 1870
Size: 73 1719 159 279 230 565 4545
Fragments arranged by size:
4545 1719 565 279 230 159 73
BamHI G'GATC_C
Cuts at: 4900 5267 4900
Size: 367 7203
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 3237 3390 4481 4891
Size: 325 217 627 764 686 153 1091 410
Cuts at: 4891 6346 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1091 764 686 627 410 325
217 153
BanII G_rGCy'C
Cuts at: 1391 2043 2886 2976 3942 4095 4779 4830 4889
Size: 652 843 90 966 153 684 51 59
Cuts at: 4889 1391
Size: 4072
Fragments arranged by size:
4072 966 843 684 652 153 90 59
51
BbeI G_GCGC'C
Cuts at: 4485 4485
Size: 7570
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4051 928
Size: 971 2152 4447
Fragments arranged by size:
4447 2152 971
BbvCI CC'TCA_GC
Cuts at: 3761 4115 3761
Size: 354 7216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2426 2460 5761 5795 1903
Size: 34 50 34 405 34 3301 34 3678
Fragments arranged by size:
3678 3301 405 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5462 6672 5462
Size: 1210 6360
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7570
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3296 3617
Size: 107 122 71 631 605 337 1287 321
Cuts at: 3617 4490 6181 136
Size: 873 1691 1525
Fragments arranged by size:
1691 1525 1287 873 631 605 337 321
122 107 71
BglII A'GATC_T
Cuts at: 7565 7565
Size: 7570
BlpI GC'TnA_GC
Cuts at: 4583 4987 4583
Size: 404 7166
Bme1580I G_kGCm'C
Cuts at: 1790 2232 2556 3068 4095 5631 6787 1790
Size: 442 324 512 1027 1536 1156 2573
Fragments arranged by size:
2573 1536 1156 1027 512 442 324
BmgBI CAC'GTC
Cuts at: 1318 2676 3724 1318
Size: 1358 1048 5164
Fragments arranged by size:
5164 1358 1048
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3557 4505 5301
Size: 416 53 83 399 24 2140 948 796
Cuts at: 5301 6259 442
Size: 958 1753
Fragments arranged by size:
2140 1753 958 948 796 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4608 4849 4608
Size: 241 7329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2720 2799 2876 3074 3263 3449 3538 3821
Size: 1481 79 77 198 189 186 89 283
Cuts at: 3821 4325 4793 6230 1239
Size: 504 468 1437 2579
Fragments arranged by size:
2579 1481 1437 504 468 283 198 189
186 89 79 77
Bpu10I CC'TnA_GC
Cuts at: 2702 3761 4115 2702
Size: 1059 354 6157
Fragments arranged by size:
6157 1059 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3119 5695 6560 6858 3119
Size: 2576 865 298 3831
Fragments arranged by size:
3831 2576 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2933 3023 3659 4329 6233 882
Size: 617 1434 90 636 670 1904 2219
Fragments arranged by size:
2219 1904 1434 670 636 617 90
BsaAI yAC'GTr
Cuts at: 493 1204 3331 3373 5361 493
Size: 711 2127 42 1988 2702
Fragments arranged by size:
2702 2127 1988 711 42
BsaBI GATnn'nnATC
Cuts at: 7564 7564
Size: 7570
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3150 3426 3687 3783
Size: 53 83 186 936 1617 276 261 96
Cuts at: 3783 3962 4056 4445 4482 5377 5759 6394 275
Size: 179 94 389 37 895 382 635 1451
Fragments arranged by size:
1617 1451 936 895 635 389 382 276
261 186 179 96 94 83 53 37
BsaWI w'CCGG_w
Cuts at: 2064 3198 4150 4470 6003 6401 6675 6822 7233
Size: 1134 952 320 1533 398 274 147 411
Cuts at: 7233 2064
Size: 2401
Fragments arranged by size:
2401 1533 1134 952 411 398 320 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 3047 3077 3374
Size: 30 165 30 708 30 906 30 297
Cuts at: 3374 3404 3536 3566 1178
Size: 30 132 30 5182
Fragments arranged by size:
5182 906 708 297 165 132 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2707 3170 3284 3395 3818 4118 1186
Size: 1521 463 114 111 423 300 4638
Fragments arranged by size:
4638 1521 463 423 300 114 111
BseYI C'CCAG_C
Cuts at: 2761 3004 3539 3705 3908 3947 4077 4082 6773
Size: 243 535 166 203 39 130 5 2691
Cuts at: 6773 7269 2761
Size: 496 3062
Fragments arranged by size:
3062 2691 535 496 243 203 166 130
39 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 2924 3587 3622 4316 4379 4424
Size: 573 648 540 663 35 694 63 45
Cuts at: 4424 1163
Size: 4309
Fragments arranged by size:
4309 694 663 648 573 540 63 45
35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2622 4074 5020 5781 6809
Size: 275 186 12 609 1452 946 761 1028
Cuts at: 6809 1540
Size: 2301
Fragments arranged by size:
2301 1452 1028 946 761 609 275 186
12
BsiHKAI G_wGCw'C
Cuts at: 2868 3068 4416 5631 5716 6787 2868
Size: 200 1348 1215 85 1071 3651
Fragments arranged by size:
3651 1348 1215 1071 200 85
BsmI GAATG_Cn'
Cuts at: 2908 5081 5174 2908
Size: 2173 93 5304
Fragments arranged by size:
5304 2173 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2686 2719 3239 4837 2686
Size: 33 520 1598 5419
Fragments arranged by size:
5419 1598 520 33
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2232 2556 2868 2886 2976 3068
Size: 399 253 189 324 312 18 90 92
Cuts at: 3068 3942 4095 4416 4779 4830 4889 5631 5716
Size: 874 153 321 363 51 59 742 85
Cuts at: 5716 6787 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 874 742 399 363 324 321
312 253 189 153 92 90 85 59
51 18
BspEI T'CCGG_A
Cuts at: 2064 4150 7233 2064
Size: 2086 3083 2401
Fragments arranged by size:
3083 2401 2086
BspHI T'CATG_A
Cuts at: 5459 7189 5459
Size: 1730 5840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2595 3045 4372 4390 4399 4741 4925 844
Size: 1751 450 1327 18 9 342 184 3489
Fragments arranged by size:
3489 1751 1327 450 342 184 18 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2127 3145 3552 5457 1394
Size: 429 304 1018 407 1905 3507
Fragments arranged by size:
3507 1905 1018 429 407 304
BsrDI GCAATG_nn'
Cuts at: 65 1248 3211 3357 3721 6065 6239 65
Size: 1183 1963 146 364 2344 174 1396
Fragments arranged by size:
2344 1963 1396 1183 364 174 146
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 3698 3875 4800 6214 6401 1700
Size: 110 198 1690 177 925 1414 187 2869
Fragments arranged by size:
2869 1690 1414 925 198 187 177 110
BsrGI T'GTAC_A
Cuts at: 96 2529 96
Size: 2433 5137
BssHII G'CGCG_C
Cuts at: 1925 4395 1925
Size: 2470 5100
BssSI C'ACGA_G
Cuts at: 3482 5630 6642 3482
Size: 2148 1012 4410
Fragments arranged by size:
4410 2148 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4411 794
Size: 3617 3953
BstBI TT'CG_AA
Cuts at: 6374 6374
Size: 7570
BstEII G'GTnAC_C
Cuts at: 1184 2285 1184
Size: 1101 6469
BstXI CCAn_nnnn'nTGG
Cuts at: 2769 2983 3799 4372 4546 4663 2769
Size: 214 816 573 174 117 5676
Fragments arranged by size:
5676 816 573 214 174 117
BstYI r'GATC_y
Cuts at: 1972 4900 5267 5652 5669 7121 7207 7219 7565
Size: 2928 367 385 17 1452 86 12 346
Cuts at: 7565 1972
Size: 1977
Fragments arranged by size:
2928 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 4128 4128
Size: 7570
BtgI C'CryG_G
Cuts at: 513 1065 2556 3312 3744 3792 4632 513
Size: 552 1491 756 432 48 840 3451
Fragments arranged by size:
3451 1491 840 756 552 432 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2040 2392 487
Size: 683 56 814 352 5665
Fragments arranged by size:
5665 814 683 352 56
BtsI GCAGTG_nn'
Cuts at: 2401 2807 5086 5880 5900 2401
Size: 406 2279 794 20 4071
Fragments arranged by size:
4071 2279 794 406 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7570
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7535
DraI TTT'AAA
Cuts at: 4874 4874
Size: 7570
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6577 809
Size: 507 5261 1802
Fragments arranged by size:
5261 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2115 2209
Size: 54 1425 50 275 18 180 105 94
Cuts at: 2209 2364 2472 2619 3072 3288 3297 3609 3771
Size: 155 108 147 453 216 9 312 162
Cuts at: 3771 3789 3843 3895 4071 5017 5906 7535 8
Size: 18 54 52 176 946 889 1629 43
Fragments arranged by size:
1629 1425 946 889 453 312 275 216
180 176 162 155 147 108 105 94
54 54 52 50 43 18 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2010 2619 4071 5017 1537
Size: 275 198 609 1452 946 4090
Fragments arranged by size:
4090 1452 946 609 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2939 3662 4277 5500 1353
Size: 1586 723 615 1223 3423
Fragments arranged by size:
3423 1586 1223 723 615
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2102 2321 3964 6157 6531 6677
Size: 1064 138 513 219 1643 2193 374 146
Cuts at: 6677 387
Size: 1280
Fragments arranged by size:
2193 1643 1280 1064 513 374 219 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2720 2799 2876 3074 3263 3302 3449
Size: 393 1088 79 77 198 189 39 147
Cuts at: 3449 3538 3821 4325 4523 4793 5633 6230 7017
Size: 89 283 504 198 270 840 597 787
Cuts at: 7017 7285 1239
Size: 268 1524
Fragments arranged by size:
1524 1088 840 787 597 504 393 283
270 268 198 198 189 147 89 79
77 39
EcoO109I rG'GnC_Cy
Cuts at: 3469 3544 3904 4091 4124 4198 4492 3469
Size: 75 360 187 33 74 294 6547
Fragments arranged by size:
6547 360 294 187 75 74 33
EcoRI G'AATT_C
Cuts at: 4879 5257 4879
Size: 378 7192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3469 3469
Size: 7570
FspI TGC'GCA
Cuts at: 3713 4814 6076 3713
Size: 1101 1262 5207
Fragments arranged by size:
5207 1262 1101
HaeII r_GCGC'y
Cuts at: 1678 3464 3623 3885 4485 4573 6717 1678
Size: 1786 159 262 600 88 2144 2531
Fragments arranged by size:
2531 2144 1786 600 262 159 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3536 3568 3815 3847 6211
Size: 32 135 32 1964 32 247 32 2364
Cuts at: 6211 6243 6285 6317 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2364 1964 247 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3960 4914 5160 669
Size: 988 281 2022 954 246 3079
Fragments arranged by size:
3079 2022 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 4658 748
Size: 3910 3660
HpaI GTT'AAC
Cuts at: 5160 5160
Size: 7570
KasI G'GCGC_C
Cuts at: 4481 4481
Size: 7570
KpnI G_GTAC'C
Cuts at: 3241 3394 4895 3241
Size: 153 1501 5916
Fragments arranged by size:
5916 1501 153
MfeI C'AATT_G
Cuts at: 5169 5169
Size: 7570
MluI A'CGCG_T
Cuts at: 7446 7446
Size: 7570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2082 2937 3204 4270 6684 6868 1305
Size: 777 855 267 1066 2414 184 2007
Fragments arranged by size:
2414 2007 1066 855 777 267 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2211 2366 3074 3290
Size: 54 1425 343 285 94 155 708 216
Cuts at: 3290 3611 3773 3791 3845 10
Size: 321 162 18 54 3735
Fragments arranged by size:
3735 1425 708 343 321 285 216 162
155 94 54 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 2384 2468 2607 2609 4544 4845
Size: 648 1134 84 84 139 2 1935 301
Cuts at: 4845 5528 5887 6046 518
Size: 683 359 159 2042
Fragments arranged by size:
2042 1935 1134 683 648 359 301 159
139 84 84 2
MspA1I CmG'CkG
Cuts at: 1606 2450 3004 3166 3324 3709 4216 4351 4453
Size: 844 554 162 158 385 507 135 102
Cuts at: 4453 4582 4986 5663 6811 7056 1606
Size: 129 404 677 1148 245 2120
Fragments arranged by size:
2120 1148 844 677 554 507 404 385
245 162 158 135 129 102
NaeI GCC'GGC
Cuts at: 2010 3700 3877 2010
Size: 1690 177 5703
Fragments arranged by size:
5703 1690 177
NarI GG'CG_CC
Cuts at: 4482 4482
Size: 7570
NcoI C'CATG_G
Cuts at: 513 1065 2556 4632 513
Size: 552 1491 2076 3451
Fragments arranged by size:
3451 2076 1491 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7570
NgoMIV G'CCGG_C
Cuts at: 2008 3698 3875 2008
Size: 1690 177 5703
Fragments arranged by size:
5703 1690 177
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7570
NotI GC'GGCC_GC
Cuts at: 5017 5017
Size: 7570
NspI r_CATG'y
Cuts at: 1132 1229 3064 4267 4928 6473 1132
Size: 97 1835 1203 661 1545 2229
Fragments arranged by size:
2229 1835 1545 1203 661 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7570
PciI A'CATG_T
Cuts at: 6469 6469
Size: 7570
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 4633 1108
Size: 1497 2028 4045
Fragments arranged by size:
4045 2028 1497
PfoI T'CCnGG_A
Cuts at: 2736 4683 2736
Size: 1947 5623
PmeI GTTT'AAAC
Cuts at: 4874 4874
Size: 7570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2303 2335 5639 5671 7152 7184 2303
Size: 32 3304 32 1481 32 2689
Fragments arranged by size:
3304 2689 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3544 3904 4124 4198 3544
Size: 360 220 74 6916
Fragments arranged by size:
6916 360 220 74
PshAI GACnn'nnGTC
Cuts at: 1613 2678 1613
Size: 1065 6505
PsiI TTA'TAA
Cuts at: 5140 5140
Size: 7570
PspOMI G'GGCC_C
Cuts at: 4091 4091
Size: 7570
PstI C_TGCA'G
Cuts at: 830 2802 3021 3735 4033 4224 4752 4922 830
Size: 1972 219 714 298 191 528 170 3478
Fragments arranged by size:
3478 1972 714 528 298 219 191 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7570
PvuII CAG'CTG
Cuts at: 3004 3324 3709 4216 4351 4582 3004
Size: 320 385 507 135 231 5992
Fragments arranged by size:
5992 507 385 320 231 135
SalI G'TCGA_C
Cuts at: 1936 3958 4912 1936
Size: 2022 954 4594
Fragments arranged by size:
4594 2022 954
SanDI GG'GwC_CC
Cuts at: 3544 3904 3544
Size: 360 7210
SbfI CC_TGCA'GG
Cuts at: 4752 4922 4752
Size: 170 7400
ScaI AGT'ACT
Cuts at: 5818 7530 5818
Size: 1712 5858
SexAI A'CCwGG_T
Cuts at: 2547 2547
Size: 7570
SfcI C'TryA_G
Cuts at: 826 1045 2161 2798 3017 3319 3401 3731 4029
Size: 219 1116 637 219 302 82 330 298
Cuts at: 4029 4220 4748 4918 6053 6734 6925 826
Size: 191 528 170 1135 681 191 1471
Fragments arranged by size:
1471 1135 1116 681 637 528 330 302
298 219 219 191 191 170 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 3296 3296
Size: 7570
SfoI GGC'GCC
Cuts at: 4483 4483
Size: 7570
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7570
SmaI CCC'GGG
Cuts at: 1872 4332 4897 1872
Size: 2460 565 4545
Fragments arranged by size:
4545 2460 565
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3134 5674 6575 6837 820
Size: 197 926 1191 2540 901 262 1553
Fragments arranged by size:
2540 1553 1191 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5361 493
Size: 4868 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7570
SphI G_CATG'C
Cuts at: 1132 4928 1132
Size: 3796 3774
SspI AAT'ATT
Cuts at: 5 52 5494 5
Size: 47 5442 2081
Fragments arranged by size:
5442 2081 47
StuI AGG'CCT
Cuts at: 1517 2441 2537 4109 1517
Size: 924 96 1572 4978
Fragments arranged by size:
4978 1572 924 96
StyI C'CwwG_G
Cuts at: 513 1065 2019 2151 2556 3514 3669 4632 5009
Size: 552 954 132 405 958 155 963 377
Cuts at: 5009 513
Size: 3074
Fragments arranged by size:
3074 963 958 954 552 405 377 155
132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2597 4330 5597 5614 5767 5952 6397
Size: 1255 1017 1733 1267 17 153 185 445
Cuts at: 6397 325
Size: 1498
Fragments arranged by size:
1733 1498 1267 1255 1017 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2529 5816 7528
Size: 274 80 33 51 1443 552 3287 1712
Cuts at: 7528 96
Size: 138
Fragments arranged by size:
3287 1712 1443 552 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2560 2592 3047 3079 7530 7562 2560
Size: 32 455 32 4451 32 2568
Fragments arranged by size:
4451 2568 455 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2492 3119 1316
Size: 1176 627 5767
Fragments arranged by size:
5767 1176 627
XbaI T'CTAG_A
Cuts at: 4906 4906
Size: 7570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4630 4630
Size: 7570
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7570
XmaI C'CCGG_G
Cuts at: 1870 4330 4895 1870
Size: 2460 565 4545
Fragments arranged by size:
4545 2460 565
XmnI GAAnn'nnTTC
Cuts at: 4613 5699 4613
Size: 1086 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3151 3427 3784 4057
Size: 53 83 186 936 1617 276 357 273
Cuts at: 4057 5378 276
Size: 1321 2468
Fragments arranged by size:
2468 1617 1321 936 357 276 273 186
83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SapI SrfI
SwaI