Restriction map for
pFN21AB8537
(Circular) (Six-base) MAPSORT of: pFN21AB8537.seq Check: 1508 from: 1 to: 7621
pFN21AB8537 7621 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3463 3535 3463
Size: 72 7549
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5431 278
Size: 53 83 186 936 3895 2468
Fragments arranged by size:
3895 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4611 4964 1937
Size: 2674 353 4594
Fragments arranged by size:
4594 2674 353
Acc65I G'GTAC_C
Cuts at: 4942 4942
Size: 7621
AclI AA'CG_TT
Cuts at: 1563 2414 5748 6121 1563
Size: 851 3334 373 3063
Fragments arranged by size:
3334 3063 851 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3864 4883 5684 7068 7336 1632
Size: 2232 1019 801 1384 268 1917
Fragments arranged by size:
2232 1917 1384 1019 801 268
AfeI AGC'GCT
Cuts at: 4681 4681
Size: 7621
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7424
AflIII A'CryG_T
Cuts at: 1203 6520 7497 1203
Size: 5317 977 1327
Fragments arranged by size:
5317 1327 977
AgeI A'CCGG_T
Cuts at: 2703 6452 2703
Size: 3749 3872
AhdI GACnn_n'nnGTC
Cuts at: 6350 6350
Size: 7621
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2183 3118 3155 3709 4379 1184
Size: 999 935 37 554 670 4426
Fragments arranged by size:
4426 999 935 670 554 37
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2269 2301 2269
Size: 32 7589
AlwNI CAG_nnn'CTG
Cuts at: 4033 4414 4723 4774 5364 6936 4033
Size: 381 309 51 590 1572 4718
Fragments arranged by size:
4718 1572 590 381 309 51
ApaI G_GGCC'C
Cuts at: 2047 2958 4319 2047
Size: 911 1361 5349
Fragments arranged by size:
5349 1361 911
ApaLI G'TGCA_C
Cuts at: 5678 6834 5678
Size: 1156 6465
ApoI r'AATT_y
Cuts at: 1493 2144 2999 3146 4480 4930 5156 5308 7417
Size: 651 855 147 1334 450 226 152 2109
Cuts at: 7417 1493
Size: 1697
Fragments arranged by size:
2109 1697 1334 855 651 450 226 152
147
AseI AT'TA_AT
Cuts at: 160 1032 6175 160
Size: 872 5143 1606
Fragments arranged by size:
5143 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7621
AvaI C'yCGr_G
Cuts at: 1870 1943 4338 4946 1870
Size: 73 2395 608 4545
Fragments arranged by size:
4545 2395 608 73
BamHI G'GATC_C
Cuts at: 2736 4951 5318 2736
Size: 2215 367 5039
Fragments arranged by size:
5039 2215 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2684 2877 3088 4942
Size: 325 217 627 249 648 193 211 1854
Cuts at: 4942 6397 618
Size: 1455 1842
Fragments arranged by size:
1854 1842 1455 648 627 325 249 217
211 193
BanII G_rGCy'C
Cuts at: 1391 2047 2136 2408 2958 4319 4940 1391
Size: 656 89 272 550 1361 621 4072
Fragments arranged by size:
4072 1361 656 621 550 272 89
BbeI G_GCGC'C
Cuts at: 2881 3092 2881
Size: 211 7410
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3667 3753 4012 4753 928
Size: 971 1768 86 259 741 3796
Fragments arranged by size:
3796 1768 971 741 259 86
BbvCI CC'TCA_GC
Cuts at: 4067 4067
Size: 7621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2009 2043 5812 5846 1903
Size: 34 72 34 3769 34 3678
Fragments arranged by size:
3769 3678 72 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5513 6723 5513
Size: 1210 6411
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7621
BfrBI ATG'CAT
Cuts at: 4844 4844
Size: 7621
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3341 3443 3938
Size: 107 122 71 631 605 1669 102 495
Cuts at: 3938 6232 136
Size: 2294 1525
Fragments arranged by size:
2294 1669 1525 631 605 495 122 107
102 71
BglII A'GATC_T
Cuts at: 2378 2428 4635 7616 2378
Size: 50 2207 2981 2383
Fragments arranged by size:
2981 2383 2207 50
BlpI GC'TnA_GC
Cuts at: 2152 4145 5038 2152
Size: 1993 893 4735
Fragments arranged by size:
4735 1993 893
Bme1580I G_kGCm'C
Cuts at: 1790 2047 2781 2958 4319 5682 6838 1790
Size: 257 734 177 1361 1363 1156 2573
Fragments arranged by size:
2573 1363 1361 1156 734 257 177
BmgBI CAC'GTC
Cuts at: 1318 2077 4015 4548 1318
Size: 759 1938 533 4391
Fragments arranged by size:
4391 1938 759 533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2411 2887 4123
Size: 416 53 83 399 24 994 476 1236
Cuts at: 4123 5352 6310 442
Size: 1229 958 1753
Fragments arranged by size:
1753 1236 1229 994 958 476 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2762 2855 4196 4733 4796 6281 1239
Size: 1181 342 93 1341 537 63 1485 2579
Fragments arranged by size:
2579 1485 1341 1181 537 342 93 63
Bpu10I CC'TnA_GC
Cuts at: 3992 4067 4683 3992
Size: 75 616 6930
Fragments arranged by size:
6930 616 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3341 4225 5746 6611 6909 3341
Size: 884 1521 865 298 4053
Fragments arranged by size:
4053 1521 884 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3131 3149 4539 6284 882
Size: 617 1632 18 1390 1745 2219
Fragments arranged by size:
2219 1745 1632 1390 617 18
BsaAI yAC'GTr
Cuts at: 493 1204 2776 5412 493
Size: 711 1572 2636 2702
Fragments arranged by size:
2702 2636 1572 711
BsaBI GATnn'nnATC
Cuts at: 7615 7615
Size: 7621
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2878 3089 4329 5428
Size: 53 83 186 936 1345 211 1240 1099
Cuts at: 5428 5810 6445 275
Size: 382 635 1451
Fragments arranged by size:
1451 1345 1240 1099 936 635 382 211
186 83 53
BsaWI w'CCGG_w
Cuts at: 2703 4659 6054 6452 6726 6873 7284 2703
Size: 1956 1395 398 274 147 411 3040
Fragments arranged by size:
3040 1956 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2212 2242 2516 2546 3422
Size: 30 165 30 809 30 274 30 876
Cuts at: 3422 3452 3563 3593 1178
Size: 30 111 30 5206
Fragments arranged by size:
5206 876 809 274 165 111 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2220 2540 2852 3479 3482 3575 3578 3760
Size: 1034 320 312 627 3 93 3 182
Cuts at: 3760 4175 1186
Size: 415 4632
Fragments arranged by size:
4632 1034 627 415 320 312 182 93
3 3
BseYI C'CCAG_C
Cuts at: 2092 2842 2862 6824 7320 2092
Size: 750 20 3962 496 2393
Fragments arranged by size:
3962 2393 750 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2756 3125 3402 3679 3705 4391 1163
Size: 573 1020 369 277 277 26 686 4393
Fragments arranged by size:
4393 1020 686 573 369 277 277 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2085 5071 5832 6860 1540
Size: 275 186 84 2986 761 1028 2301
Fragments arranged by size:
2986 2301 1028 761 275 186 84
BsiHKAI G_wGCw'C
Cuts at: 2022 2766 4151 5682 5767 6838 2022
Size: 744 1385 1531 85 1071 2805
Fragments arranged by size:
2805 1531 1385 1071 744 85
BsmI GAATG_Cn'
Cuts at: 2237 5132 5225 2237
Size: 2895 93 4633
Fragments arranged by size:
4633 2895 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2047 2136 2408 2766 2781 2958
Size: 399 232 25 89 272 358 15 177
Cuts at: 2958 4151 4319 4940 5682 5767 6838 1391
Size: 1193 168 621 742 85 1071 2174
Fragments arranged by size:
2174 1193 1071 742 621 399 358 272
232 177 168 89 85 25 15
BspEI T'CCGG_A
Cuts at: 7284 7284
Size: 7621
BspHI T'CATG_A
Cuts at: 5510 7240 5510
Size: 1730 5891
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3463 3535 4747 4976 844
Size: 2619 72 1212 229 3489
Fragments arranged by size:
3489 2619 1212 229 72
BsrBI CCG'CTC
Cuts at: 1394 1823 3280 3646 5508 1394
Size: 429 1457 366 1862 3507
Fragments arranged by size:
3507 1862 1457 429 366
BsrDI GCAATG_nn'
Cuts at: 65 1248 2058 2645 3432 3826 4176 6116 6290
Size: 1183 810 587 787 394 350 1940 174
Cuts at: 6290 65
Size: 1396
Fragments arranged by size:
1940 1396 1183 810 787 587 394 350
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2703 6265 6452 1700
Size: 110 893 3562 187 2869
Fragments arranged by size:
3562 2869 893 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7621
BssHII G'CGCG_C
Cuts at: 1925 3969 1925
Size: 2044 5577
BssSI C'ACGA_G
Cuts at: 3557 4736 5681 6693 3557
Size: 1179 945 1012 4485
Fragments arranged by size:
4485 1179 1012 945
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3478 794
Size: 2684 4937
BstBI TT'CG_AA
Cuts at: 6425 6425
Size: 7621
BstEII G'GTnAC_C
Cuts at: 1184 2723 3710 1184
Size: 1539 987 5095
Fragments arranged by size:
5095 1539 987
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 3864 2077
Size: 1787 5834
BstYI r'GATC_y
Cuts at: 1972 2378 2428 2736 4344 4635 4951 5318 5703
Size: 406 50 308 1608 291 316 367 385
Cuts at: 5703 5720 7172 7258 7270 7616 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1608 1452 406 385 367 346 316
308 291 86 50 17 12
Bsu36I CC'TnA_GG
Cuts at: 2232 4515 2232
Size: 2283 5338
BtgI C'CryG_G
Cuts at: 513 1065 2032 3403 3942 513
Size: 552 967 1371 539 4192
Fragments arranged by size:
4192 1371 967 552 539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2163 3217 487
Size: 683 56 937 1054 4891
Fragments arranged by size:
4891 1054 937 683 56
BtsI GCAGTG_nn'
Cuts at: 2167 3100 3798 4724 4795 5137 5931 5951 2167
Size: 933 698 926 71 342 794 20 3837
Fragments arranged by size:
3837 933 926 794 698 342 71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7621
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3301 3336 529
Size: 35 2737 35 4814
Fragments arranged by size:
4814 2737 35 35
DraI TTT'AAA
Cuts at: 2965 4925 2965
Size: 1960 5661
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6628 809
Size: 507 5312 1802
Fragments arranged by size:
5312 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 3390 3912
Size: 54 1425 50 275 18 252 1308 522
Cuts at: 3912 4416 5068 5957 7586 8
Size: 504 652 889 1629 43
Fragments arranged by size:
1629 1425 1308 889 652 522 504 275
252 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2082 5068 1537
Size: 275 270 2986 4090
Fragments arranged by size:
4090 2986 275 270
EarI CTCTTCn'nnn_
Cuts at: 1353 3147 3840 4388 4798 5551 1353
Size: 1794 693 548 410 753 3423
Fragments arranged by size:
3423 1794 753 693 548 410
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6208 6582 6728 387
Size: 1064 138 4619 374 146 1280
Fragments arranged by size:
4619 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2762 2855 3864 4196 4733 4796
Size: 393 788 342 93 1009 332 537 63
Cuts at: 4796 4883 5684 6281 7068 7336 1239
Size: 87 801 597 787 268 1524
Fragments arranged by size:
1524 1009 801 788 787 597 537 393
342 332 268 93 87 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2804 4893 2804
Size: 2089 5532
EcoO109I rG'GnC_Cy
Cuts at: 2043 2954 2955 3874 4303 4771 2043
Size: 911 1 919 429 468 4893
Fragments arranged by size:
4893 919 911 468 429 1
EcoRI G'AATT_C
Cuts at: 4930 5308 4930
Size: 378 7243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2342 2342
Size: 7621
FspI TGC'GCA
Cuts at: 4787 6127 4787
Size: 1340 6281
HaeII r_GCGC'y
Cuts at: 1678 2881 3092 4248 4683 6768 1678
Size: 1203 211 1156 435 2085 2531
Fragments arranged by size:
2531 2085 1203 1156 435 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2548 3038 3070 3269
Size: 32 135 32 944 32 490 32 199
Cuts at: 3269 3301 3752 3784 6262 6294 6336 6368 1373
Size: 32 451 32 2478 32 42 32 2626
Fragments arranged by size:
2626 2478 944 490 451 199 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4612 4965 5211 669
Size: 988 281 2674 353 246 3079
Fragments arranged by size:
3079 2674 988 353 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7621
HpaI GTT'AAC
Cuts at: 5211 5211
Size: 7621
KasI G'GCGC_C
Cuts at: 2877 3088 2877
Size: 211 7410
KpnI G_GTAC'C
Cuts at: 4946 4946
Size: 7621
MfeI C'AATT_G
Cuts at: 5220 5220
Size: 7621
MluI A'CGCG_T
Cuts at: 7497 7497
Size: 7621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2735 4807 6735 6919 1305
Size: 1430 2072 1928 184 2007
Fragments arranged by size:
2072 2007 1928 1430 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3392 3914 10
Size: 54 1425 343 1560 522 3717
Fragments arranged by size:
3717 1560 1425 522 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2504 3503 4457 4703 4740 5579
Size: 648 909 429 999 954 246 37 839
Cuts at: 5579 5938 6097 518
Size: 359 159 2042
Fragments arranged by size:
2042 999 954 909 839 648 429 359
246 159 37
MspA1I CmG'CkG
Cuts at: 1606 2059 3361 3693 3886 4321 4671 4861 5037
Size: 453 1302 332 193 435 350 190 176
Cuts at: 5037 5714 6862 7107 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1302 1148 677 453 435 350 332
245 193 190 176
NarI GG'CG_CC
Cuts at: 2878 3089 2878
Size: 211 7410
NcoI C'CATG_G
Cuts at: 513 1065 2032 3403 3942 513
Size: 552 967 1371 539 4192
Fragments arranged by size:
4192 1371 967 552 539
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7621
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7621
NotI GC'GGCC_GC
Cuts at: 5068 5068
Size: 7621
NruI TCG'CGA
Cuts at: 4284 4284
Size: 7621
NsiI A_TGCA'T
Cuts at: 4846 4846
Size: 7621
NspI r_CATG'y
Cuts at: 1132 1229 2092 2255 2701 3807 4261 4360 4592
Size: 97 863 163 446 1106 454 99 232
Cuts at: 4592 4786 4979 6524 1132
Size: 194 193 1545 2229
Fragments arranged by size:
2229 1545 1106 863 454 446 232 194
193 163 99 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7621
PciI A'CATG_T
Cuts at: 6520 6520
Size: 7621
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4774 1108
Size: 3666 3955
PfoI T'CCnGG_A
Cuts at: 2436 2739 2436
Size: 303 7318
PmeI GTTT'AAAC
Cuts at: 4925 4925
Size: 7621
PmlI CAC'GTG
Cuts at: 2776 2776
Size: 7621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5690 5722 7203 7235 5690
Size: 32 1481 32 6076
Fragments arranged by size:
6076 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4303 4303
Size: 7621
PshAI GACnn'nnGTC
Cuts at: 1613 2345 1613
Size: 732 6889
PsiI TTA'TAA
Cuts at: 5191 5191
Size: 7621
PspOMI G'GGCC_C
Cuts at: 2043 2954 4315 2043
Size: 911 1361 5349
Fragments arranged by size:
5349 1361 911
PstI C_TGCA'G
Cuts at: 830 2479 3546 3639 4002 4860 4973 830
Size: 1649 1067 93 363 858 113 3478
Fragments arranged by size:
3478 1649 1067 858 363 113 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7621
PvuII CAG'CTG
Cuts at: 3361 3693 4671 4861 3361
Size: 332 978 190 6121
Fragments arranged by size:
6121 978 332 190
SalI G'TCGA_C
Cuts at: 1936 4610 4963 1936
Size: 2674 353 4594
Fragments arranged by size:
4594 2674 353
SanDI GG'GwC_CC
Cuts at: 4303 4303
Size: 7621
SbfI CC_TGCA'GG
Cuts at: 3546 4973 3546
Size: 1427 6194
ScaI AGT'ACT
Cuts at: 5869 7581 5869
Size: 1712 5909
SfcI C'TryA_G
Cuts at: 826 1045 2475 3542 3635 3998 4856 4969 6104
Size: 219 1430 1067 93 363 858 113 1135
Cuts at: 6104 6785 6976 826
Size: 681 191 1471
Fragments arranged by size:
1471 1430 1135 1067 858 681 363 219
191 113 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 3938 3938
Size: 7621
SfoI GGC'GCC
Cuts at: 2879 3090 2879
Size: 211 7410
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7621
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7621
SmaI CCC'GGG
Cuts at: 1872 4340 4948 1872
Size: 2468 608 4545
Fragments arranged by size:
4545 2468 608
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3320 4204 5725 6626 6888 820
Size: 197 926 1377 884 1521 901 262 1553
Fragments arranged by size:
1553 1521 1377 926 901 884 262 197
SnaBI TAC'GTA
Cuts at: 493 5412 493
Size: 4919 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7621
SphI G_CATG'C
Cuts at: 1132 2255 3807 4360 4592 4786 4979 1132
Size: 1123 1552 553 232 194 193 3774
Fragments arranged by size:
3774 1552 1123 553 232 194 193
SspI AAT'ATT
Cuts at: 5 52 5545 5
Size: 47 5493 2081
Fragments arranged by size:
5493 2081 47
StuI AGG'CCT
Cuts at: 1517 3446 4888 1517
Size: 1929 1442 4250
Fragments arranged by size:
4250 1929 1442
StyI C'CwwG_G
Cuts at: 513 1065 2032 3106 3222 3297 3403 3441 3942
Size: 552 967 1074 116 75 106 38 501
Cuts at: 3942 4189 5060 513
Size: 247 871 3074
Fragments arranged by size:
3074 1074 967 871 552 501 247 116
106 75 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2906 2948 4521 4781 5648 5665 5818
Size: 1255 1326 42 1573 260 867 17 153
Cuts at: 5818 6003 6448 325
Size: 185 445 1498
Fragments arranged by size:
1573 1498 1326 1255 867 445 260 185
153 42 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2946 5867 7579
Size: 274 80 33 51 1443 969 2921 1712
Cuts at: 7579 96
Size: 138
Fragments arranged by size:
2921 1712 1443 969 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2212 2244 3563 3595 7581 7613 2212
Size: 32 1319 32 3986 32 2220
Fragments arranged by size:
3986 2220 1319 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2557 2570 3074 4016 1316
Size: 1241 13 504 942 4921
Fragments arranged by size:
4921 1241 942 504 13
XbaI T'CTAG_A
Cuts at: 4957 4957
Size: 7621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 3940 4120 2074
Size: 1866 180 5575
Fragments arranged by size:
5575 1866 180
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7621
XmaI C'CCGG_G
Cuts at: 1870 4338 4946 1870
Size: 2468 608 4545
Fragments arranged by size:
4545 2468 608
XmnI GAAnn'nnTTC
Cuts at: 3003 4868 5750 3003
Size: 1865 882 4874
Fragments arranged by size:
4874 1865 882
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5429 276
Size: 53 83 186 936 3895 2468
Fragments arranged by size:
3895 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI NaeI NgoMIV PacI PspXI
PsrI RsrII SacI SacII SapI SexAI SrfI SwaI