Restriction map for
pFN21AB8532
(Circular) (Six-base) MAPSORT of: pFN21AB8532.seq Check: 5716 from: 1 to: 7399
pFN21AB8532 7399 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5209 278
Size: 53 83 186 936 3673 2468
Fragments arranged by size:
3673 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 4742 1937
Size: 517 2288 4594
Fragments arranged by size:
4594 2288 517
Acc65I G'GTAC_C
Cuts at: 4720 4720
Size: 7399
AclI AA'CG_TT
Cuts at: 1563 5526 5899 1563
Size: 3963 373 3063
Fragments arranged by size:
3963 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2693 3260 3438 5462 6846 7114 1632
Size: 1061 567 178 2024 1384 268 1917
Fragments arranged by size:
2024 1917 1384 1061 567 268 178
AfeI AGC'GCT
Cuts at: 3677 3677
Size: 7399
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7202
AflIII A'CryG_T
Cuts at: 1203 6298 7275 1203
Size: 5095 977 1327
Fragments arranged by size:
5095 1327 977
AgeI A'CCGG_T
Cuts at: 4189 6230 4189
Size: 2041 5358
AhdI GACnn_n'nnGTC
Cuts at: 6128 6128
Size: 7399
AleI CACnn'nnGTG
Cuts at: 1166 2720 1166
Size: 1554 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2738 2873 1184
Size: 1554 135 5710
Fragments arranged by size:
5710 1554 135
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2093 2125 4577 4609 2093
Size: 32 2452 32 4883
Fragments arranged by size:
4883 2452 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5142 6714 5142
Size: 1572 5827
ApaI G_GGCC'C
Cuts at: 3282 3348 3282
Size: 66 7333
ApaLI G'TGCA_C
Cuts at: 2713 5456 6612 2713
Size: 2743 1156 3500
Fragments arranged by size:
3500 2743 1156
ApoI r'AATT_y
Cuts at: 1493 2019 3854 4433 4708 4934 5086 7195 1493
Size: 526 1835 579 275 226 152 2109 1697
Fragments arranged by size:
2109 1835 1697 579 526 275 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5953 160
Size: 872 4921 1606
Fragments arranged by size:
4921 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7399
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 3430 4255 4394 4597 4724 1870
Size: 73 813 674 825 139 203 127 4545
Fragments arranged by size:
4545 825 813 674 203 139 127 73
AvrII C'CTAG_G
Cuts at: 3004 3004
Size: 7399
BamHI G'GATC_C
Cuts at: 4409 4729 5096 4409
Size: 320 367 6712
Fragments arranged by size:
6712 367 320
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3992 4192 4720 6175 618
Size: 325 217 627 2205 200 528 1455 1842
Fragments arranged by size:
2205 1842 1455 627 528 325 217 200
BanII G_rGCy'C
Cuts at: 1391 2763 3201 3282 3348 3633 3645 3829 4304
Size: 1372 438 81 66 285 12 184 475
Cuts at: 4304 4718 1391
Size: 414 4072
Fragments arranged by size:
4072 1372 475 438 414 285 184 81
66 12
BbeI G_GCGC'C
Cuts at: 3996 3996
Size: 7399
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2252 2433 928
Size: 971 353 181 5894
Fragments arranged by size:
5894 971 353 181
BbvCI CC'TCA_GC
Cuts at: 3453 3453
Size: 7399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2444 2478 3313 3347 5590
Size: 34 50 34 423 34 835 34 2243
Cuts at: 5590 5624 1903
Size: 34 3678
Fragments arranged by size:
3678 2243 835 423 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5291 6501 5291
Size: 1210 6189
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7399
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4464 6010 136
Size: 107 122 71 631 605 2792 1546 1525
Fragments arranged by size:
2792 1546 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7394 7394
Size: 7399
BlpI GC'TnA_GC
Cuts at: 3864 4816 3864
Size: 952 6447
Bme1580I G_kGCm'C
Cuts at: 1790 2208 2717 2727 3282 3348 3918 4197 5460
Size: 418 509 10 555 66 570 279 1263
Cuts at: 5460 6616 1790
Size: 1156 2573
Fragments arranged by size:
2573 1263 1156 570 555 509 418 279
66 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7399
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3053 3285 3488
Size: 416 53 83 399 24 1636 232 203
Cuts at: 3488 3593 3824 5130 6088 442
Size: 105 231 1306 958 1753
Fragments arranged by size:
1753 1636 1306 958 416 399 232 231
203 105 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7399
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3664 3664
Size: 7399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2354 2580 2852 6059 1239
Size: 869 246 226 272 3207 2579
Fragments arranged by size:
3207 2579 869 272 246 226
Bpu10I CC'TnA_GC
Cuts at: 3453 4483 3453
Size: 1030 6369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 2722 3617 5524 6389 6687 2300
Size: 422 895 1907 865 298 3012
Fragments arranged by size:
3012 1907 895 865 422 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3452 3647 6062 882
Size: 617 1953 195 2415 2219
Fragments arranged by size:
2415 2219 1953 617 195
BsaAI yAC'GTr
Cuts at: 493 1204 3373 5190 493
Size: 711 2169 1817 2702
Fragments arranged by size:
2702 2169 1817 711
BsaBI GATnn'nnATC
Cuts at: 7393 7393
Size: 7399
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2627 2843 3993 5206
Size: 53 83 186 936 1094 216 1150 1213
Cuts at: 5206 5588 6223 275
Size: 382 635 1451
Fragments arranged by size:
1451 1213 1150 1094 936 635 382 216
186 83 53
BsaWI w'CCGG_w
Cuts at: 4189 5832 6230 6504 6651 7062 4189
Size: 1643 398 274 147 411 4526
Fragments arranged by size:
4526 1643 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3131 3161 4364 4394 4577
Size: 30 165 30 1728 30 1203 30 183
Cuts at: 4577 4607 1178
Size: 30 3970
Fragments arranged by size:
3970 1728 1203 183 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 3143 3155 3200 3470 4135 4537 1186
Size: 910 1047 12 45 270 665 402 4048
Fragments arranged by size:
4048 1047 910 665 402 270 45 12
BseYI C'CCAG_C
Cuts at: 2234 3152 4025 4208 4628 6602 7098 2234
Size: 918 873 183 420 1974 496 2535
Fragments arranged by size:
2535 1974 918 873 496 420 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3353 1163
Size: 573 1617 5209
Fragments arranged by size:
5209 1617 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4849 5610 6638 1540
Size: 275 186 2848 761 1028 2301
Fragments arranged by size:
2848 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 2717 2763 3645 5460 5545 6616 2256
Size: 461 46 882 1815 85 1071 3039
Fragments arranged by size:
3039 1815 1071 882 461 85 46
BsmI GAATG_Cn'
Cuts at: 2056 3220 4021 4099 4114 4910 5003 2056
Size: 1164 801 78 15 796 93 4452
Fragments arranged by size:
4452 1164 801 796 93 78 15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2256 2717 2727 2763 3201 3282
Size: 399 418 48 461 10 36 438 81
Cuts at: 3282 3348 3633 3645 3829 3918 4197 4304 4718
Size: 66 285 12 184 89 279 107 414
Cuts at: 4718 5460 5545 6616 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 461 438 418 414 399
285 279 184 107 89 85 81 66
48 36 12 10
BspEI T'CCGG_A
Cuts at: 7062 7062
Size: 7399
BspHI T'CATG_A
Cuts at: 5288 7018 5288
Size: 1730 5669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4754 844
Size: 3910 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2122 5286 1394
Size: 429 299 3164 3507
Fragments arranged by size:
3507 3164 429 299
BsrDI GCAATG_nn'
Cuts at: 65 1248 5894 6068 65
Size: 1183 4646 174 1396
Fragments arranged by size:
4646 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4019 4189 6043 6230 1700
Size: 110 2209 170 1854 187 2869
Fragments arranged by size:
2869 2209 1854 187 170 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7399
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7399
BssSI C'ACGA_G
Cuts at: 5459 6471 5459
Size: 1012 6387
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2968 794
Size: 2174 5225
BstBI TT'CG_AA
Cuts at: 6203 6203
Size: 7399
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7399
BstXI CCAn_nnnn'nTGG
Cuts at: 3310 3722 3310
Size: 412 6987
BstYI r'GATC_y
Cuts at: 1972 2329 3078 4409 4729 5096 5481 5498 6950
Size: 357 749 1331 320 367 385 17 1452
Cuts at: 6950 7036 7048 7394 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1331 749 385 367 357 346
320 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4000 4351 513
Size: 552 2935 351 3561
Fragments arranged by size:
3561 2935 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6660
Fragments arranged by size:
6660 683 56
BtsI GCAGTG_nn'
Cuts at: 2966 3483 3543 4915 5709 5729 2966
Size: 517 60 1372 794 20 4636
Fragments arranged by size:
4636 1372 794 517 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7399
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4426 4461 529
Size: 35 3862 35 3467
Fragments arranged by size:
3862 3467 35 35
DraI TTT'AAA
Cuts at: 4703 4703
Size: 7399
DraIII CAC_nnn'GTG
Cuts at: 2954 2954
Size: 7399
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6406 809
Size: 507 5090 1802
Fragments arranged by size:
5090 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2388 2400 2787
Size: 54 1425 50 275 18 558 12 387
Cuts at: 2787 2986 3360 3375 4606 4846 5735 7364 8
Size: 199 374 15 1231 240 889 1629 43
Fragments arranged by size:
1629 1425 1231 889 558 387 374 275
240 199 54 50 43 18 15 12
EagI C'GGCC_G
Cuts at: 1537 1812 4846 1537
Size: 275 3034 4090
Fragments arranged by size:
4090 3034 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3614 3815 3940 5329 1353
Size: 2261 201 125 1389 3423
Fragments arranged by size:
3423 2261 1389 201 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4422 4542 5986 6360 6506 387
Size: 1064 138 2833 120 1444 374 146 1280
Fragments arranged by size:
2833 1444 1280 1064 374 146 138 120
EcoICRI GAG'CTC
Cuts at: 2761 3643 2761
Size: 882 6517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2354 2580 2693 2852 3260 3438
Size: 393 476 246 226 113 159 408 178
Cuts at: 3438 5462 6059 6846 7114 1239
Size: 2024 597 787 268 1524
Fragments arranged by size:
2024 1524 787 597 476 408 393 268
246 226 178 159 113
EcoNI CCTnn'n_nnAGG
Cuts at: 2315 2381 2315
Size: 66 7333
EcoO109I rG'GnC_Cy
Cuts at: 2305 2707 2735 2815 3345 3896 2305
Size: 402 28 80 530 551 5808
Fragments arranged by size:
5808 551 530 402 80 28
EcoRI G'AATT_C
Cuts at: 4708 5086 4708
Size: 378 7021
EcoRV GAT'ATC
Cuts at: 2944 2944
Size: 7399
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 7399
FspI TGC'GCA
Cuts at: 2958 5905 2958
Size: 2947 4452
HaeII r_GCGC'y
Cuts at: 1678 2129 3679 3996 6546 1678
Size: 451 1550 317 2550 2531
Fragments arranged by size:
2550 2531 1550 451 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3131 3163 6040 6072 6114
Size: 32 135 32 1559 32 2877 32 42
Cuts at: 6114 6146 1373
Size: 32 2626
Fragments arranged by size:
2877 2626 1559 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2455 2578 4743 4989 669
Size: 988 281 517 123 2165 246 3079
Fragments arranged by size:
3079 2165 988 517 281 246 123
HindIII A'AGCT_T
Cuts at: 748 3776 748
Size: 3028 4371
HpaI GTT'AAC
Cuts at: 4989 4989
Size: 7399
KasI G'GCGC_C
Cuts at: 3992 3992
Size: 7399
KpnI G_GTAC'C
Cuts at: 4724 4724
Size: 7399
MfeI C'AATT_G
Cuts at: 4998 4998
Size: 7399
MluI A'CGCG_T
Cuts at: 7275 7275
Size: 7399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2096 2166 2659 3753 4437 6513 6697 1305
Size: 791 70 493 1094 684 2076 184 2007
Fragments arranged by size:
2076 2007 1094 791 684 493 184 70
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2402 10
Size: 54 1425 343 570 5007
Fragments arranged by size:
5007 1425 570 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2720 2855 3308 5357 5716 5875
Size: 648 1035 519 135 453 2049 359 159
Cuts at: 5875 518
Size: 2042
Fragments arranged by size:
2049 2042 1035 648 519 453 359 159
135
MspA1I CmG'CkG
Cuts at: 1606 3238 4815 5492 6640 6885 1606
Size: 1632 1577 677 1148 245 2120
Fragments arranged by size:
2120 1632 1577 1148 677 245
NarI GG'CG_CC
Cuts at: 3993 3993
Size: 7399
NcoI C'CATG_G
Cuts at: 513 1065 4000 4351 513
Size: 552 2935 351 3561
Fragments arranged by size:
3561 2935 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7399
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7399
NotI GC'GGCC_GC
Cuts at: 4846 4846
Size: 7399
NspI r_CATG'y
Cuts at: 1132 1229 4757 6302 1132
Size: 97 3528 1545 2229
Fragments arranged by size:
3528 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4505 1348
Size: 3157 4242
PciI A'CATG_T
Cuts at: 6298 6298
Size: 7399
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 2104 1108
Size: 939 57 6403
Fragments arranged by size:
6403 939 57
PfoI T'CCnGG_A
Cuts at: 2376 2376
Size: 7399
PmeI GTTT'AAAC
Cuts at: 4703 4703
Size: 7399
PmlI CAC'GTG
Cuts at: 3373 3373
Size: 7399
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4577 4609 5468 5500 6981 7013 4577
Size: 32 859 32 1481 32 4963
Fragments arranged by size:
4963 1481 859 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2707 2735 2707
Size: 28 7371
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7399
PsiI TTA'TAA
Cuts at: 4969 4969
Size: 7399
PspOMI G'GGCC_C
Cuts at: 3278 3344 3278
Size: 66 7333
PspXI vC'TCGA_Gb
Cuts at: 3430 3430
Size: 7399
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4676 4708 4676
Size: 32 7367
PstI C_TGCA'G
Cuts at: 830 3255 4751 830
Size: 2425 1496 3478
Fragments arranged by size:
3478 2425 1496
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7399
PvuII CAG'CTG
Cuts at: 3238 3238
Size: 7399
SacI G_AGCT'C
Cuts at: 2763 3645 2763
Size: 882 6517
SalI G'TCGA_C
Cuts at: 1936 2453 4741 1936
Size: 517 2288 4594
Fragments arranged by size:
4594 2288 517
SanDI GG'GwC_CC
Cuts at: 2735 2735
Size: 7399
SbfI CC_TGCA'GG
Cuts at: 4751 4751
Size: 7399
ScaI AGT'ACT
Cuts at: 5647 7359 5647
Size: 1712 5687
SfcI C'TryA_G
Cuts at: 826 1045 3251 4072 4084 4306 4441 4615 4747
Size: 219 2206 821 12 222 135 174 132
Cuts at: 4747 5882 6563 6754 826
Size: 1135 681 191 1471
Fragments arranged by size:
2206 1471 1135 821 681 222 219 191
174 135 132 12
SfoI GGC'GCC
Cuts at: 3994 3994
Size: 7399
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7399
SgrAI Cr'CCGG_yG
Cuts at: 1700 4189 1700
Size: 2489 4910
SmaI CCC'GGG
Cuts at: 1872 4396 4599 4726 1872
Size: 2524 203 127 4545
Fragments arranged by size:
4545 2524 203 127
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 2701 2756 3430 3632 5503
Size: 197 926 372 386 55 674 202 1871
Cuts at: 5503 6404 6666 820
Size: 901 262 1553
Fragments arranged by size:
1871 1553 926 901 674 386 372 262
202 197 55
SnaBI TAC'GTA
Cuts at: 493 5190 493
Size: 4697 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7399
SphI G_CATG'C
Cuts at: 1132 4757 1132
Size: 3625 3774
SspI AAT'ATT
Cuts at: 5 52 5323 5
Size: 47 5271 2081
Fragments arranged by size:
5271 2081 47
StuI AGG'CCT
Cuts at: 1517 3338 3749 1517
Size: 1821 411 5167
Fragments arranged by size:
5167 1821 411
StyI C'CwwG_G
Cuts at: 513 1065 2622 3004 3715 4000 4168 4351 4555
Size: 552 1557 382 711 285 168 183 204
Cuts at: 4555 4838 513
Size: 283 3074
Fragments arranged by size:
3074 1557 711 552 382 285 283 204
183 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2792 2812 3476 3865 5426 5443 5596
Size: 1255 1212 20 664 389 1561 17 153
Cuts at: 5596 5781 6226 325
Size: 185 445 1498
Fragments arranged by size:
1561 1498 1255 1212 664 445 389 185
153 20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3255 4692 5645
Size: 274 80 33 51 1443 1278 1437 953
Cuts at: 5645 7357 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1437 1278 953 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2504 2536 2719 2751 4099 4131 7359 7391 2504
Size: 32 183 32 1348 32 3228 32 2512
Fragments arranged by size:
3228 2512 1348 183 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7399
XbaI T'CTAG_A
Cuts at: 2920 4735 2920
Size: 1815 5584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2797 3370 3736 4616 2797
Size: 573 366 880 5580
Fragments arranged by size:
5580 880 573 366
XhoI C'TCGA_G
Cuts at: 1943 2756 3430 1943
Size: 813 674 5912
Fragments arranged by size:
5912 813 674
XmaI C'CCGG_G
Cuts at: 1870 4394 4597 4724 1870
Size: 2524 203 127 4545
Fragments arranged by size:
4545 2524 203 127
XmnI GAAnn'nnTTC
Cuts at: 5528 5528
Size: 7399
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5207 276
Size: 53 83 186 936 3673 2468
Fragments arranged by size:
3673 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I
FseI FspAI NaeI NgoMIV NruI NsiI PacI RsrII
SacII SapI SexAI SfiI SrfI SwaI