Restriction map for
pFN21AB8529
(Circular) (Six-base) MAPSORT of: pFN21AB8529.seq Check: 3504 from: 1 to: 7594
pFN21AB8529 7594 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4282 4282
Size: 7594
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5404 278
Size: 53 83 186 936 3868 2468
Fragments arranged by size:
3868 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4148 4937 1937
Size: 2211 789 4594
Fragments arranged by size:
4594 2211 789
Acc65I G'GTAC_C
Cuts at: 4915 4915
Size: 7594
AclI AA'CG_TT
Cuts at: 1563 5721 6094 1563
Size: 4158 373 3063
Fragments arranged by size:
4158 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2389 2675 3261 4661 4770 5657 7041 7309
Size: 757 286 586 1400 109 887 1384 268
Cuts at: 7309 1632
Size: 1917
Fragments arranged by size:
1917 1400 1384 887 757 586 286 268
109
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7397
AflIII A'CryG_T
Cuts at: 1203 3123 4036 6493 7470 1203
Size: 1920 913 2457 977 1327
Fragments arranged by size:
2457 1920 1327 977 913
AgeI A'CCGG_T
Cuts at: 6425 6425
Size: 7594
AhdI GACnn_n'nnGTC
Cuts at: 4144 6323 4144
Size: 2179 5415
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7594
AlwNI CAG_nnn'CTG
Cuts at: 2630 4572 5337 6909 2630
Size: 1942 765 1572 3315
Fragments arranged by size:
3315 1942 1572 765
ApaLI G'TGCA_C
Cuts at: 5651 6807 5651
Size: 1156 6438
ApoI r'AATT_y
Cuts at: 1493 2666 3271 3319 3819 3838 4099 4343 4886
Size: 1173 605 48 500 19 261 244 543
Cuts at: 4886 4903 5129 5281 7390 1493
Size: 17 226 152 2109 1697
Fragments arranged by size:
2109 1697 1173 605 543 500 261 244
226 152 48 19 17
AseI AT'TA_AT
Cuts at: 160 1032 3312 6148 160
Size: 872 2280 2836 1606
Fragments arranged by size:
2836 2280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
AvaI C'yCGr_G
Cuts at: 1870 1943 4919 1870
Size: 73 2976 4545
Fragments arranged by size:
4545 2976 73
BamHI G'GATC_C
Cuts at: 4924 5291 4924
Size: 367 7227
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 4915 6370 618
Size: 325 217 627 312 2816 1455 1842
Fragments arranged by size:
2816 1842 1455 627 325 312 217
BanII G_rGCy'C
Cuts at: 1391 4363 4913 1391
Size: 2972 550 4072
Fragments arranged by size:
4072 2972 550
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6623
BbvCI CC'TCA_GC
Cuts at: 3149 3149
Size: 7594
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5785 5819 1903
Size: 34 3848 34 3678
Fragments arranged by size:
3848 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2018 4529 5486 6696 2018
Size: 2511 957 1210 2916
Fragments arranged by size:
2916 2511 1210 957
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7594
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6205 136
Size: 107 122 71 631 605 4533 1525
Fragments arranged by size:
4533 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2371 7589 2371
Size: 5218 2376
BlpI GC'TnA_GC
Cuts at: 5011 5011
Size: 7594
Bme1580I G_kGCm'C
Cuts at: 1790 2241 5655 6811 1790
Size: 451 3414 1156 2573
Fragments arranged by size:
3414 2573 1156 451
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7594
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2982 4582 4607
Size: 416 53 83 399 24 1565 1600 25
Cuts at: 4607 5325 6283 442
Size: 718 958 1753
Fragments arranged by size:
1753 1600 1565 958 718 416 399 83
53 25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2460 2565 3630 4190 4549 6254 1239
Size: 1221 105 1065 560 359 1705 2579
Fragments arranged by size:
2579 1705 1221 1065 560 359 105
Bpu10I CC'TnA_GC
Cuts at: 3149 3149
Size: 7594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5719 6584 6882 5719
Size: 865 298 6431
Fragments arranged by size:
6431 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6257 882
Size: 617 4758 2219
Fragments arranged by size:
4758 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5385 493
Size: 711 4181 2702
Fragments arranged by size:
4181 2702 711
BsaBI GATnn'nnATC
Cuts at: 7588 7588
Size: 7594
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 5401 5783 6418
Size: 53 83 186 936 477 3391 382 635
Cuts at: 6418 275
Size: 1451
Fragments arranged by size:
3391 1451 936 635 477 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3130 4114 4718 6027 6425 6699 6846 7257 3130
Size: 984 604 1309 398 274 147 411 3467
Fragments arranged by size:
3467 1309 984 604 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4236 4266 1178
Size: 30 165 30 2833 30 4506
Fragments arranged by size:
4506 2833 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 1186
Size: 1618 5976
BseYI C'CCAG_C
Cuts at: 6797 7293 6797
Size: 496 7098
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 1163
Size: 573 771 6250
Fragments arranged by size:
6250 771 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5044 5805 6833 1540
Size: 275 186 3043 761 1028 2301
Fragments arranged by size:
3043 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4363 5655 5740 6811 4363
Size: 1292 85 1071 5146
Fragments arranged by size:
5146 1292 1071 85
BsmI GAATG_Cn'
Cuts at: 2431 5105 5198 2431
Size: 2674 93 4827
Fragments arranged by size:
4827 2674 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 4363 4913 5655 5740 6811 1391
Size: 399 451 2122 550 742 85 1071 2174
Fragments arranged by size:
2174 2122 1071 742 550 451 399 85
BspEI T'CCGG_A
Cuts at: 3130 7257 3130
Size: 4127 3467
BspHI T'CATG_A
Cuts at: 5483 7213 5483
Size: 1730 5864
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2460 2531 3566 3993 4282 4949 844
Size: 1616 71 1035 427 289 667 3489
Fragments arranged by size:
3489 1616 1035 667 427 289 71
BsrBI CCG'CTC
Cuts at: 1394 1823 5481 1394
Size: 429 3658 3507
Fragments arranged by size:
3658 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2233 2295 3519 6089 6263 65
Size: 1183 985 62 1224 2570 174 1396
Fragments arranged by size:
2570 1396 1224 1183 985 174 62
BsrFI r'CCGG_y
Cuts at: 1700 1810 3968 6238 6425 1700
Size: 110 2158 2270 187 2869
Fragments arranged by size:
2869 2270 2158 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7594
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7594
BssSI C'ACGA_G
Cuts at: 4338 5654 6666 4338
Size: 1316 1012 5266
Fragments arranged by size:
5266 1316 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2626 794
Size: 1832 5762
BstBI TT'CG_AA
Cuts at: 3259 6398 3259
Size: 3139 4455
BstEII G'GTnAC_C
Cuts at: 1184 2447 3808 1184
Size: 1263 1361 4970
Fragments arranged by size:
4970 1361 1263
BstYI r'GATC_y
Cuts at: 1972 2371 2531 4924 5291 5676 5693 7145 7231
Size: 399 160 2393 367 385 17 1452 86
Cuts at: 7231 7243 7589 1972
Size: 12 346 1977
Fragments arranged by size:
2393 1977 1452 399 385 367 346 160
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3032 3357 3032
Size: 325 7269
BtgI C'CryG_G
Cuts at: 513 1065 2004 4133 513
Size: 552 939 2129 3974
Fragments arranged by size:
3974 2129 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6855
Fragments arranged by size:
6855 683 56
BtsI GCAGTG_nn'
Cuts at: 2325 2739 3658 5110 5904 5924 2325
Size: 414 919 1452 794 20 3995
Fragments arranged by size:
3995 1452 919 794 414 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7594
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3729 3764 529
Size: 35 3165 35 4359
Fragments arranged by size:
4359 3165 35 35
DraI TTT'AAA
Cuts at: 3427 3783 4057 4898 3427
Size: 356 274 841 6123
Fragments arranged by size:
6123 841 356 274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2831 6601 809
Size: 507 1515 3770 1802
Fragments arranged by size:
3770 1802 1515 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5041 5930 7559
Size: 54 1425 50 275 18 3211 889 1629
Cuts at: 7559 8
Size: 43
Fragments arranged by size:
3211 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5041 1537
Size: 275 3229 4090
Fragments arranged by size:
4090 3229 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2822 4360 5524 1353
Size: 1469 1538 1164 3423
Fragments arranged by size:
3423 1538 1469 1164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6181 6555 6701 387
Size: 1064 138 4592 374 146 1280
Fragments arranged by size:
4592 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4361 4361
Size: 7594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2389 2460 2565 2675 3261 3630 4190
Size: 393 757 71 105 110 586 369 560
Cuts at: 4190 4549 4661 4770 5657 6254 7041 7309 1239
Size: 359 112 109 887 597 787 268 1524
Fragments arranged by size:
1524 887 787 757 597 586 560 393
369 359 268 112 110 109 105 71
EcoRI G'AATT_C
Cuts at: 2666 3271 3319 4099 4343 4903 5281 2666
Size: 605 48 780 244 560 378 4979
Fragments arranged by size:
4979 780 605 560 378 244 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 3869 4413 2095
Size: 1774 544 5276
Fragments arranged by size:
5276 1774 544
FspI TGC'GCA
Cuts at: 6100 6100
Size: 7594
HaeII r_GCGC'y
Cuts at: 1678 6741 1678
Size: 5063 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3452 3484 3501 3533 3705
Size: 32 135 32 1880 32 17 32 172
Cuts at: 3705 3737 4130 4162 4236 4253 4268 4285 6235
Size: 32 393 32 74 17 15 17 1950
Cuts at: 6235 6267 6309 6341 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1950 1880 393 172 135 74 42
32 32 32 32 32 32 32 32
17 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 2970 3166 4938 5184 669
Size: 988 281 445 587 196 1772 246 3079
Fragments arranged by size:
3079 1772 988 587 445 281 246 196
HindIII A'AGCT_T
Cuts at: 748 2498 3707 4412 748
Size: 1750 1209 705 3930
Fragments arranged by size:
3930 1750 1209 705
HpaI GTT'AAC
Cuts at: 2970 5184 2970
Size: 2214 5380
KpnI G_GTAC'C
Cuts at: 4919 4919
Size: 7594
MfeI C'AATT_G
Cuts at: 4197 5193 4197
Size: 996 6598
MluI A'CGCG_T
Cuts at: 7470 7470
Size: 7594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 2390 2872 2938 3400 6708 6892 1305
Size: 816 269 482 66 462 3308 184 2007
Fragments arranged by size:
3308 2007 816 482 462 269 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5772
Fragments arranged by size:
5772 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2855 5552 5911 6070 518
Size: 648 1689 2697 359 159 2042
Fragments arranged by size:
2697 2042 1689 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3446 5010 5687 6835 7080 1606
Size: 1840 1564 677 1148 245 2120
Fragments arranged by size:
2120 1840 1564 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4133 513
Size: 552 939 2129 3974
Fragments arranged by size:
3974 2129 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7594
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7594
NotI GC'GGCC_GC
Cuts at: 5041 5041
Size: 7594
NspI r_CATG'y
Cuts at: 1132 1229 3127 4040 4952 6497 1132
Size: 97 1898 913 912 1545 2229
Fragments arranged by size:
2229 1898 1545 913 912 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7594
PciI A'CATG_T
Cuts at: 3123 4036 6493 3123
Size: 913 2457 4224
Fragments arranged by size:
4224 2457 913
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2438 3653 4572 1108
Size: 1330 1215 919 4130
Fragments arranged by size:
4130 1330 1215 919
PfoI T'CCnGG_A
Cuts at: 2437 2743 4687 4762 2437
Size: 306 1944 75 5269
Fragments arranged by size:
5269 1944 306 75
PmeI GTTT'AAAC
Cuts at: 4898 4898
Size: 7594
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3999 4031 5663 5695 7176 7208 3999
Size: 32 1632 32 1481 32 4385
Fragments arranged by size:
4385 1632 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7594
PsiI TTA'TAA
Cuts at: 5164 5164
Size: 7594
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4026 4058 4026
Size: 32 7562
PstI C_TGCA'G
Cuts at: 830 2457 2510 3529 3577 4279 4498 4946 830
Size: 1627 53 1019 48 702 219 448 3478
Fragments arranged by size:
3478 1627 1019 702 448 219 53 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7594
PvuII CAG'CTG
Cuts at: 3446 3446
Size: 7594
SacI G_AGCT'C
Cuts at: 4363 4363
Size: 7594
SalI G'TCGA_C
Cuts at: 1936 4936 1936
Size: 3000 4594
SbfI CC_TGCA'GG
Cuts at: 4279 4946 4279
Size: 667 6927
ScaI AGT'ACT
Cuts at: 5842 7554 5842
Size: 1712 5882
SfcI C'TryA_G
Cuts at: 826 1045 2453 2506 3096 3525 3573 4275 4494
Size: 219 1408 53 590 429 48 702 219
Cuts at: 4494 4942 6077 6758 6949 826
Size: 448 1135 681 191 1471
Fragments arranged by size:
1471 1408 1135 702 681 590 448 429
219 219 191 53 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7594
SmaI CCC'GGG
Cuts at: 1872 4921 1872
Size: 3049 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5698 6599 6861 820
Size: 197 926 3755 901 262 1553
Fragments arranged by size:
3755 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5385 493
Size: 4892 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7594
SphI G_CATG'C
Cuts at: 1132 4952 1132
Size: 3820 3774
SspI AAT'ATT
Cuts at: 5 52 2780 5518 5
Size: 47 2728 2738 2081
Fragments arranged by size:
2738 2728 2081 47
StuI AGG'CCT
Cuts at: 1517 4329 1517
Size: 2812 4782
StyI C'CwwG_G
Cuts at: 513 1065 2004 4133 4707 5033 513
Size: 552 939 2129 574 326 3074
Fragments arranged by size:
3074 2129 939 574 552 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2611 4589 5621 5638 5791 5976 6421
Size: 1255 1031 1978 1032 17 153 185 445
Cuts at: 6421 325
Size: 1498
Fragments arranged by size:
1978 1498 1255 1032 1031 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5840 7552 96
Size: 274 80 33 51 1443 3863 1712 138
Fragments arranged by size:
3863 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4665 4697 7554 7586 4665
Size: 32 2857 32 4673
Fragments arranged by size:
4673 2857 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4250 1316
Size: 2934 4660
XbaI T'CTAG_A
Cuts at: 4930 4930
Size: 7594
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7594
XmaI C'CCGG_G
Cuts at: 1870 4919 1870
Size: 3049 4545
XmnI GAAnn'nnTTC
Cuts at: 2078 2572 4014 5723 2078
Size: 494 1442 1709 3949
Fragments arranged by size:
3949 1709 1442 494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5402 276
Size: 53 83 186 936 3868 2468
Fragments arranged by size:
3868 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI