(Circular) (Six-base) MAPSORT of: pFN21AB8525.seq Check: 7692 from: 1 to: 7588 pFN21AB8525 7588 bp With 182 enzymes: SgfI * October 20, 2009 14:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4260 5398 278 Size: 53 83 186 936 2724 1138 2468 Fragments arranged by size: 2724 2468 1138 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4931 1937 Size: 2994 4594 Acc65I G'GTAC_C Cuts at: 2130 4909 2130 Size: 2779 4809 AclI AA'CG_TT Cuts at: 1563 5715 6088 1563 Size: 4152 373 3063 Fragments arranged by size: 4152 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3086 3132 3614 5651 7035 7303 1632 Size: 1454 46 482 2037 1384 268 1917 Fragments arranged by size: 2037 1917 1454 1384 482 268 46 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7391 AflIII A'CryG_T Cuts at: 1203 3856 6487 7464 1203 Size: 2653 2631 977 1327 Fragments arranged by size: 2653 2631 1327 977 AgeI A'CCGG_T Cuts at: 6419 6419 Size: 7588 AhdI GACnn_n'nnGTC Cuts at: 6317 6317 Size: 7588 AleI CACnn'nnGTG Cuts at: 1166 2759 1166 Size: 1593 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4465 1184 Size: 3281 4307 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3536 3568 3536 Size: 32 7556 AlwNI CAG_nnn'CTG Cuts at: 2289 3739 5331 6903 2289 Size: 1450 1592 1572 2974 Fragments arranged by size: 2974 1592 1572 1450 ApaI G_GGCC'C Cuts at: 2232 2232 Size: 7588 ApaLI G'TGCA_C Cuts at: 5645 6801 5645 Size: 1156 6432 ApoI r'AATT_y Cuts at: 1493 3338 4897 5123 5275 7384 1493 Size: 1845 1559 226 152 2109 1697 Fragments arranged by size: 2109 1845 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 6142 160 Size: 872 5110 1606 Fragments arranged by size: 5110 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7588 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2145 2379 4590 4913 1870 Size: 73 104 98 234 2211 323 4545 Fragments arranged by size: 4545 2211 323 234 104 98 73 BamHI G'GATC_C Cuts at: 4918 5285 4918 Size: 367 7221 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2130 3403 4779 4909 Size: 325 217 627 239 104 1273 1376 130 Cuts at: 4909 6364 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1376 1273 627 325 239 217 130 104 BanII G_rGCy'C Cuts at: 1391 2232 2380 2637 3951 4839 4907 1391 Size: 841 148 257 1314 888 68 4072 Fragments arranged by size: 4072 1314 888 841 257 148 68 BbeI G_GCGC'C Cuts at: 2030 4783 2030 Size: 2753 4835 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2950 3928 4069 4118 4634 928 Size: 971 1051 978 141 49 516 3882 Fragments arranged by size: 3882 1051 978 971 516 141 49 BbvCI CC'TCA_GC Cuts at: 2766 2766 Size: 7588 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5779 5813 1903 Size: 34 3842 34 3678 Fragments arranged by size: 3842 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5480 6690 5480 Size: 1210 6378 BclI T'GATC_A Cuts at: 1548 4405 4584 1548 Size: 2857 179 4552 Fragments arranged by size: 4552 2857 179 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 3428 6199 Size: 107 122 71 631 605 377 1379 2771 Cuts at: 6199 136 Size: 1525 Fragments arranged by size: 2771 1525 1379 631 605 377 122 107 71 BglII A'GATC_T Cuts at: 3210 7583 3210 Size: 4373 3215 BlpI GC'TnA_GC Cuts at: 4850 5005 4850 Size: 155 7433 Bme1580I G_kGCm'C Cuts at: 1790 2232 2527 3054 4569 5649 6805 1790 Size: 442 295 527 1515 1080 1156 2573 Fragments arranged by size: 2573 1515 1156 1080 527 442 295 BmgBI CAC'GTC Cuts at: 1318 2599 1318 Size: 1281 6307 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5319 6277 442 Size: 416 53 83 399 24 3902 958 1753 Fragments arranged by size: 3902 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7588 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3583 4131 4747 3583 Size: 548 616 6424 Fragments arranged by size: 6424 616 548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 2794 3002 3480 3509 3784 4276 4298 Size: 1373 182 208 478 29 275 492 22 Cuts at: 4298 4736 6248 1239 Size: 438 1512 2579 Fragments arranged by size: 2579 1512 1373 492 478 438 275 208 182 29 22 Bpu10I CC'TnA_GC Cuts at: 2766 4833 2766 Size: 2067 5521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2621 5713 6578 6876 2621 Size: 3092 865 298 3333 Fragments arranged by size: 3333 3092 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 6251 882 Size: 617 2868 1884 2219 Fragments arranged by size: 2868 2219 1884 617 BsaAI yAC'GTr Cuts at: 493 1204 5379 493 Size: 711 4175 2702 Fragments arranged by size: 4175 2702 711 BsaBI GATnn'nnATC Cuts at: 7582 7582 Size: 7588 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 4257 4780 5395 Size: 53 83 186 936 494 2230 523 615 Cuts at: 5395 5777 6412 275 Size: 382 635 1451 Fragments arranged by size: 2230 1451 936 635 615 523 494 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2391 4209 6021 6419 6693 6840 7251 2391 Size: 1818 1812 398 274 147 411 2728 Fragments arranged by size: 2728 1818 1812 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2022 2052 2367 2397 2422 Size: 30 165 30 619 30 315 30 25 Cuts at: 2422 2452 4649 4679 1178 Size: 30 2197 30 4087 Fragments arranged by size: 4087 2197 619 315 165 30 30 30 30 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3395 3477 4525 4670 4673 4736 1186 Size: 2209 82 1048 145 3 63 4038 Fragments arranged by size: 4038 2209 1048 145 82 63 3 BseYI C'CCAG_C Cuts at: 2282 2362 3724 4272 4351 6791 7287 2282 Size: 80 1362 548 79 2440 496 2583 Fragments arranged by size: 2583 2440 1362 548 496 80 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3217 4190 1163 Size: 573 1481 973 4561 Fragments arranged by size: 4561 1481 973 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3472 4846 5038 5799 6827 1540 Size: 275 186 1471 1374 192 761 1028 2301 Fragments arranged by size: 2301 1471 1374 1028 761 275 192 186 BsiHKAI G_wGCw'C Cuts at: 2459 2637 4560 5649 5734 6805 2459 Size: 178 1923 1089 85 1071 3242 Fragments arranged by size: 3242 1923 1089 1071 178 85 BsmI GAATG_Cn' Cuts at: 5099 5192 5099 Size: 93 7495 BsmBI CGTCTCn'nnnn_ Cuts at: 3155 3793 4264 3155 Size: 638 471 6479 Fragments arranged by size: 6479 638 471 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2380 2459 2527 2637 3054 3951 Size: 399 442 148 79 68 110 417 897 Cuts at: 3951 4560 4569 4839 4907 5649 5734 6805 1391 Size: 609 9 270 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 897 742 609 442 417 399 270 148 110 85 79 68 68 9 BspEI T'CCGG_A Cuts at: 7251 7251 Size: 7588 BspHI T'CATG_A Cuts at: 5477 7207 5477 Size: 1730 5858 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4943 844 Size: 4099 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2541 4773 5475 1394 Size: 429 718 2232 702 3507 Fragments arranged by size: 3507 2232 718 702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3546 4282 4707 6083 6257 65 Size: 1183 2298 736 425 1376 174 1396 Fragments arranged by size: 2298 1396 1376 1183 736 425 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2832 4845 6232 6419 1700 Size: 110 1022 2013 1387 187 2869 Fragments arranged by size: 2869 2013 1387 1022 187 110 BsrGI T'GTAC_A Cuts at: 96 2514 3138 96 Size: 2418 624 4546 Fragments arranged by size: 4546 2418 624 BssHII G'CGCG_C Cuts at: 1925 2506 1925 Size: 581 7007 BssSI C'ACGA_G Cuts at: 5648 6660 5648 Size: 1012 6576 BstAPI GCAn_nnn'nTGC Cuts at: 794 4505 794 Size: 3711 3877 BstBI TT'CG_AA Cuts at: 6392 6392 Size: 7588 BstEII G'GTnAC_C Cuts at: 1184 3956 1184 Size: 2772 4816 BstXI CCAn_nnnn'nTGG Cuts at: 2761 2761 Size: 7588 BstYI r'GATC_y Cuts at: 1972 3040 3210 4918 5285 5670 5687 7139 7225 Size: 1068 170 1708 367 385 17 1452 86 Cuts at: 7225 7237 7583 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1708 1452 1068 385 367 346 170 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3378 4242 3378 Size: 864 6724 BtgI C'CryG_G Cuts at: 513 1065 2574 513 Size: 552 1509 5527 Fragments arranged by size: 5527 1509 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6849 Fragments arranged by size: 6849 683 56 BtsI GCAGTG_nn' Cuts at: 2470 2517 4458 5104 5898 5918 2470 Size: 47 1941 646 794 20 4140 Fragments arranged by size: 4140 1941 794 646 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7588 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7553 DraI TTT'AAA Cuts at: 4892 4892 Size: 7588 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6595 809 Size: 507 5279 1802 Fragments arranged by size: 5279 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2549 3469 Size: 54 1425 50 275 18 420 299 920 Cuts at: 3469 3571 5035 5924 7553 8 Size: 102 1464 889 1629 43 Fragments arranged by size: 1629 1464 1425 920 889 420 299 275 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3469 5035 1537 Size: 275 1657 1566 4090 Fragments arranged by size: 4090 1657 1566 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 3553 5518 1353 Size: 686 1514 1965 3423 Fragments arranged by size: 3423 1965 1514 686 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 3011 6175 6549 6695 387 Size: 1064 138 441 981 3164 374 146 1280 Fragments arranged by size: 3164 1280 1064 981 441 374 146 138 EcoICRI GAG'CTC Cuts at: 2635 2635 Size: 7588 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2612 2794 3002 3086 3132 3480 3509 Size: 393 980 182 208 84 46 348 29 Cuts at: 3509 3614 3784 4276 4298 4736 5651 6248 7035 Size: 105 170 492 22 438 915 597 787 Cuts at: 7035 7303 1239 Size: 268 1524 Fragments arranged by size: 1524 980 915 787 597 492 438 393 348 268 208 182 170 105 84 46 29 22 EcoO109I rG'GnC_Cy Cuts at: 2137 2228 2356 3717 4813 4826 2137 Size: 91 128 1361 1096 13 4899 Fragments arranged by size: 4899 1361 1096 128 91 13 EcoRI G'AATT_C Cuts at: 4897 5275 4897 Size: 378 7210 FspI TGC'GCA Cuts at: 4577 6094 4577 Size: 1517 6071 HaeII r_GCGC'y Cuts at: 1678 2030 2591 3073 4783 6735 1678 Size: 352 561 482 1710 1952 2531 Fragments arranged by size: 2531 1952 1710 561 482 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4390 4422 4649 4681 6229 Size: 32 135 32 2818 32 227 32 1548 Cuts at: 6229 6261 6303 6335 1373 Size: 32 42 32 2626 Fragments arranged by size: 2818 2626 1548 227 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4932 5178 669 Size: 988 281 2994 246 3079 Fragments arranged by size: 3079 2994 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7588 HpaI GTT'AAC Cuts at: 5178 5178 Size: 7588 KasI G'GCGC_C Cuts at: 2026 4779 2026 Size: 2753 4835 KpnI G_GTAC'C Cuts at: 2134 4913 2134 Size: 2779 4809 MfeI C'AATT_G Cuts at: 3199 5187 3199 Size: 1988 5600 MluI A'CGCG_T Cuts at: 7464 7464 Size: 7588 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3345 4587 4656 6702 6886 1305 Size: 2040 1242 69 2046 184 2007 Fragments arranged by size: 2046 2040 2007 1242 184 69 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 2551 3573 10 Size: 54 1425 343 420 299 1022 4025 Fragments arranged by size: 4025 1425 1022 420 343 299 54 MslI CAynn'nnrTG Cuts at: 518 1166 2729 2759 5546 5905 6064 518 Size: 648 1563 30 2787 359 159 2042 Fragments arranged by size: 2787 2042 1563 648 359 159 30 MspA1I CmG'CkG Cuts at: 1606 2289 2576 3047 3409 3594 3728 4276 5004 Size: 683 287 471 362 185 134 548 728 Cuts at: 5004 5681 6829 7074 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 728 683 677 548 471 362 287 245 185 134 NarI GG'CG_CC Cuts at: 2027 4780 2027 Size: 2753 4835 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7036 NdeI CA'TA_TG Cuts at: 387 387 Size: 7588 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7588 NotI GC'GGCC_GC Cuts at: 5035 5035 Size: 7588 NspI r_CATG'y Cuts at: 1132 1229 3860 4946 6491 1132 Size: 97 2631 1086 1545 2229 Fragments arranged by size: 2631 2229 1545 1086 97 PasI CC'CwG_GG Cuts at: 1348 2112 3943 4821 1348 Size: 764 1831 878 4115 Fragments arranged by size: 4115 1831 878 764 PciI A'CATG_T Cuts at: 3856 6487 3856 Size: 2631 4957 PflMI CCAn_nnn'nTGG Cuts at: 1108 2416 3739 4059 1108 Size: 1308 1323 320 4637 Fragments arranged by size: 4637 1323 1308 320 PfoI T'CCnGG_A Cuts at: 2261 2426 3396 4292 2261 Size: 165 970 896 5557 Fragments arranged by size: 5557 970 896 165 PmeI GTTT'AAAC Cuts at: 4892 4892 Size: 7588 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5657 5689 7170 7202 5657 Size: 32 1481 32 6043 Fragments arranged by size: 6043 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2356 3717 4826 2356 Size: 1361 1109 5118 Fragments arranged by size: 5118 1361 1109 PshAI GACnn'nnGTC Cuts at: 1613 3740 1613 Size: 2127 5461 PsiI TTA'TAA Cuts at: 4884 5158 4884 Size: 274 7314 PspOMI G'GGCC_C Cuts at: 2228 2228 Size: 7588 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3158 3190 3158 Size: 32 7556 PstI C_TGCA'G Cuts at: 830 2744 4940 830 Size: 1914 2196 3478 Fragments arranged by size: 3478 2196 1914 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7588 PvuII CAG'CTG Cuts at: 3047 3728 4276 3047 Size: 681 548 6359 Fragments arranged by size: 6359 681 548 SacI G_AGCT'C Cuts at: 2637 2637 Size: 7588 SacII CC_GC'GG Cuts at: 2577 2577 Size: 7588 SalI G'TCGA_C Cuts at: 1936 4930 1936 Size: 2994 4594 SanDI GG'GwC_CC Cuts at: 3717 4826 3717 Size: 1109 6479 SbfI CC_TGCA'GG Cuts at: 4940 4940 Size: 7588 ScaI AGT'ACT Cuts at: 5836 7548 5836 Size: 1712 5876 SexAI A'CCwGG_T Cuts at: 2151 2151 Size: 7588 SfcI C'TryA_G Cuts at: 826 1045 2437 2740 4936 6071 6752 6943 826 Size: 219 1392 303 2196 1135 681 191 1471 Fragments arranged by size: 2196 1471 1392 1135 681 303 219 191 SfoI GGC'GCC Cuts at: 2028 4781 2028 Size: 2753 4835 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7588 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7588 SmaI CCC'GGG Cuts at: 1872 2049 2381 4915 1872 Size: 177 332 2534 4545 Fragments arranged by size: 4545 2534 332 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 2636 5692 6593 6855 820 Size: 197 926 693 3056 901 262 1553 Fragments arranged by size: 3056 1553 926 901 693 262 197 SnaBI TAC'GTA Cuts at: 493 5379 493 Size: 4886 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7588 SphI G_CATG'C Cuts at: 1132 4946 1132 Size: 3814 3774 SspI AAT'ATT Cuts at: 5 52 5512 5 Size: 47 5460 2081 Fragments arranged by size: 5460 2081 47 StuI AGG'CCT Cuts at: 1517 2564 1517 Size: 1047 6541 StyI C'CwwG_G Cuts at: 513 1065 2232 3432 3672 4230 5027 513 Size: 552 1167 1200 240 558 797 3074 Fragments arranged by size: 3074 1200 1167 797 558 552 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2296 2880 3174 4800 5615 5632 5785 Size: 1255 716 584 294 1626 815 17 153 Cuts at: 5785 5970 6415 325 Size: 185 445 1498 Fragments arranged by size: 1626 1498 1255 815 716 584 445 294 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 3138 5834 Size: 274 80 33 51 1443 537 624 2696 Cuts at: 5834 7546 96 Size: 1712 138 Fragments arranged by size: 2696 1712 1443 624 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 2367 2378 2399 2410 7548 7580 2022 Size: 32 313 11 21 11 5138 32 2030 Fragments arranged by size: 5138 2030 313 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 4633 1316 Size: 3317 4271 XbaI T'CTAG_A Cuts at: 4924 4924 Size: 7588 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3430 3430 Size: 7588 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7588 XmaI C'CCGG_G Cuts at: 1870 2047 2379 4913 1870 Size: 177 332 2534 4545 Fragments arranged by size: 4545 2534 332 177 XmnI GAAnn'nnTTC Cuts at: 2339 5717 2339 Size: 3378 4210 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4258 5396 276 Size: 53 83 186 936 2724 1138 2468 Fragments arranged by size: 2724 2468 1138 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SapI SfiI SrfI SwaI