Restriction map for
pFN21AB8525
(Circular) (Six-base) MAPSORT of: pFN21AB8525.seq Check: 7692 from: 1 to: 7588
pFN21AB8525 7588 bp
With 182 enzymes: SgfI *
October 20, 2009 14:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4260 5398 278
Size: 53 83 186 936 2724 1138 2468
Fragments arranged by size:
2724 2468 1138 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4931 1937
Size: 2994 4594
Acc65I G'GTAC_C
Cuts at: 2130 4909 2130
Size: 2779 4809
AclI AA'CG_TT
Cuts at: 1563 5715 6088 1563
Size: 4152 373 3063
Fragments arranged by size:
4152 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3086 3132 3614 5651 7035 7303 1632
Size: 1454 46 482 2037 1384 268 1917
Fragments arranged by size:
2037 1917 1454 1384 482 268 46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7391
AflIII A'CryG_T
Cuts at: 1203 3856 6487 7464 1203
Size: 2653 2631 977 1327
Fragments arranged by size:
2653 2631 1327 977
AgeI A'CCGG_T
Cuts at: 6419 6419
Size: 7588
AhdI GACnn_n'nnGTC
Cuts at: 6317 6317
Size: 7588
AleI CACnn'nnGTG
Cuts at: 1166 2759 1166
Size: 1593 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4465 1184
Size: 3281 4307
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 7556
AlwNI CAG_nnn'CTG
Cuts at: 2289 3739 5331 6903 2289
Size: 1450 1592 1572 2974
Fragments arranged by size:
2974 1592 1572 1450
ApaI G_GGCC'C
Cuts at: 2232 2232
Size: 7588
ApaLI G'TGCA_C
Cuts at: 5645 6801 5645
Size: 1156 6432
ApoI r'AATT_y
Cuts at: 1493 3338 4897 5123 5275 7384 1493
Size: 1845 1559 226 152 2109 1697
Fragments arranged by size:
2109 1845 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6142 160
Size: 872 5110 1606
Fragments arranged by size:
5110 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7588
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2145 2379 4590 4913 1870
Size: 73 104 98 234 2211 323 4545
Fragments arranged by size:
4545 2211 323 234 104 98 73
BamHI G'GATC_C
Cuts at: 4918 5285 4918
Size: 367 7221
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2130 3403 4779 4909
Size: 325 217 627 239 104 1273 1376 130
Cuts at: 4909 6364 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1376 1273 627 325 239 217
130 104
BanII G_rGCy'C
Cuts at: 1391 2232 2380 2637 3951 4839 4907 1391
Size: 841 148 257 1314 888 68 4072
Fragments arranged by size:
4072 1314 888 841 257 148 68
BbeI G_GCGC'C
Cuts at: 2030 4783 2030
Size: 2753 4835
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2950 3928 4069 4118 4634 928
Size: 971 1051 978 141 49 516 3882
Fragments arranged by size:
3882 1051 978 971 516 141 49
BbvCI CC'TCA_GC
Cuts at: 2766 2766
Size: 7588
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5779 5813 1903
Size: 34 3842 34 3678
Fragments arranged by size:
3842 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5480 6690 5480
Size: 1210 6378
BclI T'GATC_A
Cuts at: 1548 4405 4584 1548
Size: 2857 179 4552
Fragments arranged by size:
4552 2857 179
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 3428 6199
Size: 107 122 71 631 605 377 1379 2771
Cuts at: 6199 136
Size: 1525
Fragments arranged by size:
2771 1525 1379 631 605 377 122 107
71
BglII A'GATC_T
Cuts at: 3210 7583 3210
Size: 4373 3215
BlpI GC'TnA_GC
Cuts at: 4850 5005 4850
Size: 155 7433
Bme1580I G_kGCm'C
Cuts at: 1790 2232 2527 3054 4569 5649 6805 1790
Size: 442 295 527 1515 1080 1156 2573
Fragments arranged by size:
2573 1515 1156 1080 527 442 295
BmgBI CAC'GTC
Cuts at: 1318 2599 1318
Size: 1281 6307
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5319 6277 442
Size: 416 53 83 399 24 3902 958 1753
Fragments arranged by size:
3902 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7588
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3583 4131 4747 3583
Size: 548 616 6424
Fragments arranged by size:
6424 616 548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 2794 3002 3480 3509 3784 4276 4298
Size: 1373 182 208 478 29 275 492 22
Cuts at: 4298 4736 6248 1239
Size: 438 1512 2579
Fragments arranged by size:
2579 1512 1373 492 478 438 275 208
182 29 22
Bpu10I CC'TnA_GC
Cuts at: 2766 4833 2766
Size: 2067 5521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2621 5713 6578 6876 2621
Size: 3092 865 298 3333
Fragments arranged by size:
3333 3092 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 6251 882
Size: 617 2868 1884 2219
Fragments arranged by size:
2868 2219 1884 617
BsaAI yAC'GTr
Cuts at: 493 1204 5379 493
Size: 711 4175 2702
Fragments arranged by size:
4175 2702 711
BsaBI GATnn'nnATC
Cuts at: 7582 7582
Size: 7588
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 4257 4780 5395
Size: 53 83 186 936 494 2230 523 615
Cuts at: 5395 5777 6412 275
Size: 382 635 1451
Fragments arranged by size:
2230 1451 936 635 615 523 494 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2391 4209 6021 6419 6693 6840 7251 2391
Size: 1818 1812 398 274 147 411 2728
Fragments arranged by size:
2728 1818 1812 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 2367 2397 2422
Size: 30 165 30 619 30 315 30 25
Cuts at: 2422 2452 4649 4679 1178
Size: 30 2197 30 4087
Fragments arranged by size:
4087 2197 619 315 165 30 30 30
30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3395 3477 4525 4670 4673 4736 1186
Size: 2209 82 1048 145 3 63 4038
Fragments arranged by size:
4038 2209 1048 145 82 63 3
BseYI C'CCAG_C
Cuts at: 2282 2362 3724 4272 4351 6791 7287 2282
Size: 80 1362 548 79 2440 496 2583
Fragments arranged by size:
2583 2440 1362 548 496 80 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3217 4190 1163
Size: 573 1481 973 4561
Fragments arranged by size:
4561 1481 973 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3472 4846 5038 5799 6827 1540
Size: 275 186 1471 1374 192 761 1028 2301
Fragments arranged by size:
2301 1471 1374 1028 761 275 192 186
BsiHKAI G_wGCw'C
Cuts at: 2459 2637 4560 5649 5734 6805 2459
Size: 178 1923 1089 85 1071 3242
Fragments arranged by size:
3242 1923 1089 1071 178 85
BsmI GAATG_Cn'
Cuts at: 5099 5192 5099
Size: 93 7495
BsmBI CGTCTCn'nnnn_
Cuts at: 3155 3793 4264 3155
Size: 638 471 6479
Fragments arranged by size:
6479 638 471
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2380 2459 2527 2637 3054 3951
Size: 399 442 148 79 68 110 417 897
Cuts at: 3951 4560 4569 4839 4907 5649 5734 6805 1391
Size: 609 9 270 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 897 742 609 442 417 399
270 148 110 85 79 68 68 9
BspEI T'CCGG_A
Cuts at: 7251 7251
Size: 7588
BspHI T'CATG_A
Cuts at: 5477 7207 5477
Size: 1730 5858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4943 844
Size: 4099 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2541 4773 5475 1394
Size: 429 718 2232 702 3507
Fragments arranged by size:
3507 2232 718 702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3546 4282 4707 6083 6257 65
Size: 1183 2298 736 425 1376 174 1396
Fragments arranged by size:
2298 1396 1376 1183 736 425 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2832 4845 6232 6419 1700
Size: 110 1022 2013 1387 187 2869
Fragments arranged by size:
2869 2013 1387 1022 187 110
BsrGI T'GTAC_A
Cuts at: 96 2514 3138 96
Size: 2418 624 4546
Fragments arranged by size:
4546 2418 624
BssHII G'CGCG_C
Cuts at: 1925 2506 1925
Size: 581 7007
BssSI C'ACGA_G
Cuts at: 5648 6660 5648
Size: 1012 6576
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4505 794
Size: 3711 3877
BstBI TT'CG_AA
Cuts at: 6392 6392
Size: 7588
BstEII G'GTnAC_C
Cuts at: 1184 3956 1184
Size: 2772 4816
BstXI CCAn_nnnn'nTGG
Cuts at: 2761 2761
Size: 7588
BstYI r'GATC_y
Cuts at: 1972 3040 3210 4918 5285 5670 5687 7139 7225
Size: 1068 170 1708 367 385 17 1452 86
Cuts at: 7225 7237 7583 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1708 1452 1068 385 367 346 170
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3378 4242 3378
Size: 864 6724
BtgI C'CryG_G
Cuts at: 513 1065 2574 513
Size: 552 1509 5527
Fragments arranged by size:
5527 1509 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6849
Fragments arranged by size:
6849 683 56
BtsI GCAGTG_nn'
Cuts at: 2470 2517 4458 5104 5898 5918 2470
Size: 47 1941 646 794 20 4140
Fragments arranged by size:
4140 1941 794 646 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7588
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7553
DraI TTT'AAA
Cuts at: 4892 4892
Size: 7588
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6595 809
Size: 507 5279 1802
Fragments arranged by size:
5279 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2549 3469
Size: 54 1425 50 275 18 420 299 920
Cuts at: 3469 3571 5035 5924 7553 8
Size: 102 1464 889 1629 43
Fragments arranged by size:
1629 1464 1425 920 889 420 299 275
102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3469 5035 1537
Size: 275 1657 1566 4090
Fragments arranged by size:
4090 1657 1566 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 3553 5518 1353
Size: 686 1514 1965 3423
Fragments arranged by size:
3423 1965 1514 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 3011 6175 6549 6695 387
Size: 1064 138 441 981 3164 374 146 1280
Fragments arranged by size:
3164 1280 1064 981 441 374 146 138
EcoICRI GAG'CTC
Cuts at: 2635 2635
Size: 7588
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2612 2794 3002 3086 3132 3480 3509
Size: 393 980 182 208 84 46 348 29
Cuts at: 3509 3614 3784 4276 4298 4736 5651 6248 7035
Size: 105 170 492 22 438 915 597 787
Cuts at: 7035 7303 1239
Size: 268 1524
Fragments arranged by size:
1524 980 915 787 597 492 438 393
348 268 208 182 170 105 84 46
29 22
EcoO109I rG'GnC_Cy
Cuts at: 2137 2228 2356 3717 4813 4826 2137
Size: 91 128 1361 1096 13 4899
Fragments arranged by size:
4899 1361 1096 128 91 13
EcoRI G'AATT_C
Cuts at: 4897 5275 4897
Size: 378 7210
FspI TGC'GCA
Cuts at: 4577 6094 4577
Size: 1517 6071
HaeII r_GCGC'y
Cuts at: 1678 2030 2591 3073 4783 6735 1678
Size: 352 561 482 1710 1952 2531
Fragments arranged by size:
2531 1952 1710 561 482 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4390 4422 4649 4681 6229
Size: 32 135 32 2818 32 227 32 1548
Cuts at: 6229 6261 6303 6335 1373
Size: 32 42 32 2626
Fragments arranged by size:
2818 2626 1548 227 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4932 5178 669
Size: 988 281 2994 246 3079
Fragments arranged by size:
3079 2994 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7588
HpaI GTT'AAC
Cuts at: 5178 5178
Size: 7588
KasI G'GCGC_C
Cuts at: 2026 4779 2026
Size: 2753 4835
KpnI G_GTAC'C
Cuts at: 2134 4913 2134
Size: 2779 4809
MfeI C'AATT_G
Cuts at: 3199 5187 3199
Size: 1988 5600
MluI A'CGCG_T
Cuts at: 7464 7464
Size: 7588
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3345 4587 4656 6702 6886 1305
Size: 2040 1242 69 2046 184 2007
Fragments arranged by size:
2046 2040 2007 1242 184 69
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 2551 3573 10
Size: 54 1425 343 420 299 1022 4025
Fragments arranged by size:
4025 1425 1022 420 343 299 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2729 2759 5546 5905 6064 518
Size: 648 1563 30 2787 359 159 2042
Fragments arranged by size:
2787 2042 1563 648 359 159 30
MspA1I CmG'CkG
Cuts at: 1606 2289 2576 3047 3409 3594 3728 4276 5004
Size: 683 287 471 362 185 134 548 728
Cuts at: 5004 5681 6829 7074 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 728 683 677 548 471 362
287 245 185 134
NarI GG'CG_CC
Cuts at: 2027 4780 2027
Size: 2753 4835
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7036
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7588
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7588
NotI GC'GGCC_GC
Cuts at: 5035 5035
Size: 7588
NspI r_CATG'y
Cuts at: 1132 1229 3860 4946 6491 1132
Size: 97 2631 1086 1545 2229
Fragments arranged by size:
2631 2229 1545 1086 97
PasI CC'CwG_GG
Cuts at: 1348 2112 3943 4821 1348
Size: 764 1831 878 4115
Fragments arranged by size:
4115 1831 878 764
PciI A'CATG_T
Cuts at: 3856 6487 3856
Size: 2631 4957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2416 3739 4059 1108
Size: 1308 1323 320 4637
Fragments arranged by size:
4637 1323 1308 320
PfoI T'CCnGG_A
Cuts at: 2261 2426 3396 4292 2261
Size: 165 970 896 5557
Fragments arranged by size:
5557 970 896 165
PmeI GTTT'AAAC
Cuts at: 4892 4892
Size: 7588
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5657 5689 7170 7202 5657
Size: 32 1481 32 6043
Fragments arranged by size:
6043 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2356 3717 4826 2356
Size: 1361 1109 5118
Fragments arranged by size:
5118 1361 1109
PshAI GACnn'nnGTC
Cuts at: 1613 3740 1613
Size: 2127 5461
PsiI TTA'TAA
Cuts at: 4884 5158 4884
Size: 274 7314
PspOMI G'GGCC_C
Cuts at: 2228 2228
Size: 7588
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 7556
PstI C_TGCA'G
Cuts at: 830 2744 4940 830
Size: 1914 2196 3478
Fragments arranged by size:
3478 2196 1914
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7588
PvuII CAG'CTG
Cuts at: 3047 3728 4276 3047
Size: 681 548 6359
Fragments arranged by size:
6359 681 548
SacI G_AGCT'C
Cuts at: 2637 2637
Size: 7588
SacII CC_GC'GG
Cuts at: 2577 2577
Size: 7588
SalI G'TCGA_C
Cuts at: 1936 4930 1936
Size: 2994 4594
SanDI GG'GwC_CC
Cuts at: 3717 4826 3717
Size: 1109 6479
SbfI CC_TGCA'GG
Cuts at: 4940 4940
Size: 7588
ScaI AGT'ACT
Cuts at: 5836 7548 5836
Size: 1712 5876
SexAI A'CCwGG_T
Cuts at: 2151 2151
Size: 7588
SfcI C'TryA_G
Cuts at: 826 1045 2437 2740 4936 6071 6752 6943 826
Size: 219 1392 303 2196 1135 681 191 1471
Fragments arranged by size:
2196 1471 1392 1135 681 303 219 191
SfoI GGC'GCC
Cuts at: 2028 4781 2028
Size: 2753 4835
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7588
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7588
SmaI CCC'GGG
Cuts at: 1872 2049 2381 4915 1872
Size: 177 332 2534 4545
Fragments arranged by size:
4545 2534 332 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2636 5692 6593 6855 820
Size: 197 926 693 3056 901 262 1553
Fragments arranged by size:
3056 1553 926 901 693 262 197
SnaBI TAC'GTA
Cuts at: 493 5379 493
Size: 4886 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7588
SphI G_CATG'C
Cuts at: 1132 4946 1132
Size: 3814 3774
SspI AAT'ATT
Cuts at: 5 52 5512 5
Size: 47 5460 2081
Fragments arranged by size:
5460 2081 47
StuI AGG'CCT
Cuts at: 1517 2564 1517
Size: 1047 6541
StyI C'CwwG_G
Cuts at: 513 1065 2232 3432 3672 4230 5027 513
Size: 552 1167 1200 240 558 797 3074
Fragments arranged by size:
3074 1200 1167 797 558 552 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2296 2880 3174 4800 5615 5632 5785
Size: 1255 716 584 294 1626 815 17 153
Cuts at: 5785 5970 6415 325
Size: 185 445 1498
Fragments arranged by size:
1626 1498 1255 815 716 584 445 294
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 3138 5834
Size: 274 80 33 51 1443 537 624 2696
Cuts at: 5834 7546 96
Size: 1712 138
Fragments arranged by size:
2696 1712 1443 624 537 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 2367 2378 2399 2410 7548 7580 2022
Size: 32 313 11 21 11 5138 32 2030
Fragments arranged by size:
5138 2030 313 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 4633 1316
Size: 3317 4271
XbaI T'CTAG_A
Cuts at: 4924 4924
Size: 7588
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3430 3430
Size: 7588
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7588
XmaI C'CCGG_G
Cuts at: 1870 2047 2379 4913 1870
Size: 177 332 2534 4545
Fragments arranged by size:
4545 2534 332 177
XmnI GAAnn'nnTTC
Cuts at: 2339 5717 2339
Size: 3378 4210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4258 5396 276
Size: 53 83 186 936 2724 1138 2468
Fragments arranged by size:
2724 2468 1138 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspXI RsrII SapI SfiI
SrfI SwaI