Restriction map for
pFN21AB8521
(Circular) (Six-base) MAPSORT of: pFN21AB8521.seq Check: 1185 from: 1 to: 7573
pFN21AB8521 7573 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4462 4509 4462
Size: 47 7526
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5383 278
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4916 1937
Size: 2979 4594
Acc65I G'GTAC_C
Cuts at: 3719 3921 4894 3719
Size: 202 973 6398
Fragments arranged by size:
6398 973 202
AclI AA'CG_TT
Cuts at: 1563 5700 6073 1563
Size: 4137 373 3063
Fragments arranged by size:
4137 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2439 4013 4127 4429 4631 4707 5636 7020
Size: 807 1574 114 302 202 76 929 1384
Cuts at: 7020 7288 1632
Size: 268 1917
Fragments arranged by size:
1917 1574 1384 929 807 302 268 202
114 76
AfeI AGC'GCT
Cuts at: 4034 4034
Size: 7573
AflII C'TTAA_G
Cuts at: 820 1017 3146 820
Size: 197 2129 5247
Fragments arranged by size:
5247 2129 197
AflIII A'CryG_T
Cuts at: 1203 2160 6472 7449 1203
Size: 957 4312 977 1327
Fragments arranged by size:
4312 1327 977 957
AgeI A'CCGG_T
Cuts at: 6404 6404
Size: 7573
AhdI GACnn_n'nnGTC
Cuts at: 6302 6302
Size: 7573
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7573
AlwNI CAG_nnn'CTG
Cuts at: 3556 3991 4089 4210 4222 5316 6888 3556
Size: 435 98 121 12 1094 1572 4241
Fragments arranged by size:
4241 1572 1094 435 121 98 12
ApaI G_GGCC'C
Cuts at: 3769 4587 3769
Size: 818 6755
ApaLI G'TGCA_C
Cuts at: 4463 5630 6786 4463
Size: 1167 1156 5250
Fragments arranged by size:
5250 1167 1156
ApoI r'AATT_y
Cuts at: 1493 2413 2975 4882 5108 5260 7369 1493
Size: 920 562 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 920 562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6127 160
Size: 872 5095 1606
Fragments arranged by size:
5095 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
AvaI C'yCGr_G
Cuts at: 1870 1943 2644 2891 3650 4898 1870
Size: 73 701 247 759 1248 4545
Fragments arranged by size:
4545 1248 759 701 247 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2169 2202 2911 2944 3703 3736 2169
Size: 33 709 33 759 33 6006
Fragments arranged by size:
6006 759 709 33 33 33
BamHI G'GATC_C
Cuts at: 4903 5270 4903
Size: 367 7206
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2036 2713 3719 3921
Size: 325 217 627 242 7 677 1006 202
Cuts at: 3921 4537 4894 6349 618
Size: 616 357 1455 1842
Fragments arranged by size:
1842 1455 1006 677 627 616 357 325
242 217 202 7
BanII G_rGCy'C
Cuts at: 1391 2870 3769 4331 4587 4859 4892 1391
Size: 1479 899 562 256 272 33 4072
Fragments arranged by size:
4072 1479 899 562 272 256 33
BbeI G_GCGC'C
Cuts at: 2033 2040 2033
Size: 7 7566
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 2844 3740 4695 928
Size: 971 567 378 896 955 3806
Fragments arranged by size:
3806 971 955 896 567 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5764 5798 1903
Size: 34 50 34 3743 34 3678
Fragments arranged by size:
3743 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5465 6675 5465
Size: 1210 6363
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2896 3101 3251
Size: 107 122 71 631 605 1224 205 150
Cuts at: 3251 6184 136
Size: 2933 1525
Fragments arranged by size:
2933 1525 1224 631 605 205 150 122
107 71
BglII A'GATC_T
Cuts at: 7568 7568
Size: 7573
BlpI GC'TnA_GC
Cuts at: 4301 4990 4301
Size: 689 6884
Bme1580I G_kGCm'C
Cuts at: 1790 2657 3769 3915 4467 4542 4587 4598 5634
Size: 867 1112 146 552 75 45 11 1036
Cuts at: 5634 6790 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1112 1036 867 552 146 75
45 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7573
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2475 3724 4871
Size: 416 53 83 399 24 1058 1249 1147
Cuts at: 4871 5304 6262 442
Size: 433 958 1753
Fragments arranged by size:
1753 1249 1147 1058 958 433 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2039 2416 2541 3269 3485 4160 6233
Size: 791 9 377 125 728 216 675 2073
Cuts at: 6233 1239
Size: 2579
Fragments arranged by size:
2579 2073 791 728 675 377 216 125
9
Bpu10I CC'TnA_GC
Cuts at: 2864 4023 2864
Size: 1159 6414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3032 3197 3281 4382 5698 6563 6861 3032
Size: 165 84 1101 1316 865 298 3744
Fragments arranged by size:
3744 1316 1101 865 298 165 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2519 2743 3750 4136 6236 882
Size: 617 1020 224 1007 386 2100 2219
Fragments arranged by size:
2219 2100 1020 1007 617 386 224
BsaAI yAC'GTr
Cuts at: 493 1204 3056 4462 5364 493
Size: 711 1852 1406 902 2702
Fragments arranged by size:
2702 1852 1406 902 711
BsaBI GATnn'nnATC
Cuts at: 7567 7567
Size: 7573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2037 3451 4169
Size: 53 83 186 936 497 7 1414 718
Cuts at: 4169 5380 5762 6397 275
Size: 1211 382 635 1451
Fragments arranged by size:
1451 1414 1211 936 718 635 497 382
186 83 53 7
BsaWI w'CCGG_w
Cuts at: 3563 4802 6006 6404 6678 6825 7236 3563
Size: 1239 1204 398 274 147 411 3900
Fragments arranged by size:
3900 1239 1204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 3827 3857 1178
Size: 30 165 30 603 30 1791 30 4894
Fragments arranged by size:
4894 1791 603 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2570 2573 2602 3104 3125 3233 3488
Size: 841 543 3 29 502 21 108 255
Cuts at: 3488 3670 3824 3839 3965 4175 4207 4733 4736
Size: 182 154 15 126 210 32 526 3
Cuts at: 4736 4745 4748 1186
Size: 9 3 4011
Fragments arranged by size:
4011 841 543 526 502 255 210 182
154 126 108 32 29 21 15 9
3 3 3
BseYI C'CCAG_C
Cuts at: 2687 4215 4361 4586 4808 6776 7272 2687
Size: 1528 146 225 222 1968 496 2988
Fragments arranged by size:
2988 1968 1528 496 225 222 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2601 3078 4217 4331 1163
Size: 573 865 477 1139 114 4405
Fragments arranged by size:
4405 1139 865 573 477 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5023 5784 6812 1540
Size: 275 186 3022 761 1028 2301
Fragments arranged by size:
3022 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2345 2870 3432 4284 4467 4500 5634 5719 6790
Size: 525 562 852 183 33 1134 85 1071
Cuts at: 6790 2345
Size: 3128
Fragments arranged by size:
3128 1134 1071 852 562 525 183 85
33
BsmI GAATG_Cn'
Cuts at: 3595 5084 5177 3595
Size: 1489 93 5991
Fragments arranged by size:
5991 1489 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4191 4191
Size: 7573
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2345 2657 2870 3432 3769 3915 4284
Size: 399 555 312 213 562 337 146 369
Cuts at: 4284 4331 4467 4500 4542 4587 4598 4859 4892
Size: 47 136 33 42 45 11 261 33
Cuts at: 4892 5634 5719 6790 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 555 399 369 337
312 261 213 146 136 85 47 45
42 33 33 11
BspEI T'CCGG_A
Cuts at: 3563 4802 7236 3563
Size: 1239 2434 3900
Fragments arranged by size:
3900 2434 1239
BspHI T'CATG_A
Cuts at: 4650 5462 7192 4650
Size: 812 1730 5031
Fragments arranged by size:
5031 1730 812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4353 4462 4509 4928 844
Size: 3509 109 47 419 3489
Fragments arranged by size:
3509 3489 419 109 47
BsrBI CCG'CTC
Cuts at: 1394 1823 3223 3513 4228 5460 1394
Size: 429 1400 290 715 1232 3507
Fragments arranged by size:
3507 1400 1232 715 429 290
BsrDI GCAATG_nn'
Cuts at: 65 1248 4246 6068 6242 65
Size: 1183 2998 1822 174 1396
Fragments arranged by size:
2998 1822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3389 3546 3702 4150 4313 4832 6217
Size: 110 1579 157 156 448 163 519 1385
Cuts at: 6217 6404 1700
Size: 187 2869
Fragments arranged by size:
2869 1579 1385 519 448 187 163 157
156 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7573
BssHII G'CGCG_C
Cuts at: 1925 3750 1925
Size: 1825 5748
BssSI C'ACGA_G
Cuts at: 3340 5633 6645 3340
Size: 2293 1012 4268
Fragments arranged by size:
4268 2293 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4635 794
Size: 3841 3732
BstBI TT'CG_AA
Cuts at: 6377 6377
Size: 7573
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7573
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 3385 2059
Size: 1326 6247
BstYI r'GATC_y
Cuts at: 1972 4206 4903 5270 5655 5672 7124 7210 7222
Size: 2234 697 367 385 17 1452 86 12
Cuts at: 7222 7568 1972
Size: 346 1977
Fragments arranged by size:
2234 1977 1452 697 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3557 3557
Size: 7573
BtgI C'CryG_G
Cuts at: 513 1065 2058 2615 3916 4322 4578 513
Size: 552 993 557 1301 406 256 3508
Fragments arranged by size:
3508 1301 993 557 552 406 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4113 487
Size: 683 56 2887 3947
Fragments arranged by size:
3947 2887 683 56
BtsI GCAGTG_nn'
Cuts at: 4282 5089 5883 5903 4282
Size: 807 794 20 5952
Fragments arranged by size:
5952 807 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7538
DraI TTT'AAA
Cuts at: 4877 4877
Size: 7573
DraIII CAC_nnn'GTG
Cuts at: 4865 4865
Size: 7573
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6580 809
Size: 507 5264 1802
Fragments arranged by size:
5264 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2335 2449 3387
Size: 54 1425 50 275 18 505 114 938
Cuts at: 3387 4672 5020 5909 7538 8
Size: 1285 348 889 1629 43
Fragments arranged by size:
1629 1425 1285 938 889 505 348 275
114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5020 1537
Size: 275 3208 4090
Fragments arranged by size:
4090 3208 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2881 3129 3279 3300 3816 4607 4722 5503
Size: 1528 248 150 21 516 791 115 781
Cuts at: 5503 1353
Size: 3423
Fragments arranged by size:
3423 1528 791 781 516 248 150 115
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3212 3971 6160 6534 6680 387
Size: 1064 138 1623 759 2189 374 146 1280
Fragments arranged by size:
2189 1623 1280 1064 759 374 146 138
EcoICRI GAG'CTC
Cuts at: 2868 2868
Size: 7573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2039 2416 2439 2541 3269 3485
Size: 393 398 9 377 23 102 728 216
Cuts at: 3485 4013 4127 4160 4429 4631 4707 5636 6233
Size: 528 114 33 269 202 76 929 597
Cuts at: 6233 7020 7288 1239
Size: 787 268 1524
Fragments arranged by size:
1524 929 787 728 597 528 398 393
377 269 268 216 202 114 102 76
33 23 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3929 4022 3929
Size: 93 7480
EcoO109I rG'GnC_Cy
Cuts at: 2291 3373 4583 2291
Size: 1082 1210 5281
Fragments arranged by size:
5281 1210 1082
EcoRI G'AATT_C
Cuts at: 4882 5260 4882
Size: 378 7195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 7573
FspI TGC'GCA
Cuts at: 4093 6079 4093
Size: 1986 5587
HaeII r_GCGC'y
Cuts at: 1678 2033 2040 2128 4036 6720 1678
Size: 355 7 88 1908 2684 2531
Fragments arranged by size:
2684 2531 1908 355 88 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 3827 3859 6214
Size: 32 135 32 575 32 1648 32 2355
Cuts at: 6214 6246 6288 6320 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2355 1648 575 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2281 4917 5163 669
Size: 988 281 343 2636 246 3079
Fragments arranged by size:
3079 2636 988 343 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7573
HpaI GTT'AAC
Cuts at: 5163 5163
Size: 7573
KasI G'GCGC_C
Cuts at: 2029 2036 2029
Size: 7 7566
KpnI G_GTAC'C
Cuts at: 3723 3925 4898 3723
Size: 202 973 6398
Fragments arranged by size:
6398 973 202
MfeI C'AATT_G
Cuts at: 5172 5172
Size: 7573
MluI A'CGCG_T
Cuts at: 7449 7449
Size: 7573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2657 3434 6687 6871 1305
Size: 1352 777 3253 184 2007
Fragments arranged by size:
3253 2007 1352 777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2337 2451 10
Size: 54 1425 343 505 114 5132
Fragments arranged by size:
5132 1425 505 343 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 4239 5531 5890 6049 518
Size: 648 891 2182 1292 359 159 2042
Fragments arranged by size:
2182 2042 1292 891 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3208 3487 4219 4324 4625 4989 5666 6814
Size: 1602 279 732 105 301 364 677 1148
Cuts at: 6814 7059 1606
Size: 245 2120
Fragments arranged by size:
2120 1602 1148 732 677 364 301 279
245 105
NaeI GCC'GGC
Cuts at: 3391 3548 4152 3391
Size: 157 604 6812
Fragments arranged by size:
6812 604 157
NarI GG'CG_CC
Cuts at: 2030 2037 2030
Size: 7 7566
NcoI C'CATG_G
Cuts at: 513 1065 2058 3916 513
Size: 552 993 1858 4170
Fragments arranged by size:
4170 1858 993 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7573
NgoMIV G'CCGG_C
Cuts at: 3389 3546 4150 3389
Size: 157 604 6812
Fragments arranged by size:
6812 604 157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7573
NotI GC'GGCC_GC
Cuts at: 5020 5020
Size: 7573
NspI r_CATG'y
Cuts at: 1132 1229 2164 4636 4931 6476 1132
Size: 97 935 2472 295 1545 2229
Fragments arranged by size:
2472 2229 1545 935 295 97
PasI CC'CwG_GG
Cuts at: 1348 3762 1348
Size: 2414 5159
PciI A'CATG_T
Cuts at: 2160 6472 2160
Size: 4312 3261
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4005 1108
Size: 2897 4676
PfoI T'CCnGG_A
Cuts at: 2518 3783 4137 4266 4275 4761 2518
Size: 1265 354 129 9 486 5330
Fragments arranged by size:
5330 1265 486 354 129 9
PmeI GTTT'AAAC
Cuts at: 4877 4877
Size: 7573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5642 5674 7155 7187 5642
Size: 32 1481 32 6028
Fragments arranged by size:
6028 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2291 2291
Size: 7573
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7573
PsiI TTA'TAA
Cuts at: 5143 5143
Size: 7573
PspOMI G'GGCC_C
Cuts at: 3765 4583 3765
Size: 818 6755
PstI C_TGCA'G
Cuts at: 830 3482 3687 4119 4925 830
Size: 2652 205 432 806 3478
Fragments arranged by size:
3478 2652 806 432 205
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7573
PvuII CAG'CTG
Cuts at: 3487 4219 3487
Size: 732 6841
RsrII CG'GwC_CG
Cuts at: 3456 3456
Size: 7573
SacI G_AGCT'C
Cuts at: 2870 2870
Size: 7573
SacII CC_GC'GG
Cuts at: 4325 4325
Size: 7573
SalI G'TCGA_C
Cuts at: 1936 4915 1936
Size: 2979 4594
SanDI GG'GwC_CC
Cuts at: 2291 2291
Size: 7573
SbfI CC_TGCA'GG
Cuts at: 4925 4925
Size: 7573
ScaI AGT'ACT
Cuts at: 5821 7533 5821
Size: 1712 5861
SfcI C'TryA_G
Cuts at: 826 1045 2991 3478 3683 4115 4478 4921 6056
Size: 219 1946 487 205 432 363 443 1135
Cuts at: 6056 6737 6928 826
Size: 681 191 1471
Fragments arranged by size:
1946 1471 1135 681 487 443 432 363
219 205 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3101 3101
Size: 7573
SfoI GGC'GCC
Cuts at: 2031 2038 2031
Size: 7 7566
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7573
SmaI CCC'GGG
Cuts at: 1872 4900 1872
Size: 3028 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3047 3146 3212 3260 4397 5677
Size: 197 926 1104 99 66 48 1137 1280
Cuts at: 5677 6578 6840 820
Size: 901 262 1553
Fragments arranged by size:
1553 1280 1137 1104 926 901 262 197
99 66 48
SnaBI TAC'GTA
Cuts at: 493 5364 493
Size: 4871 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7573
SphI G_CATG'C
Cuts at: 1132 4931 1132
Size: 3799 3774
SspI AAT'ATT
Cuts at: 5 52 5497 5
Size: 47 5445 2081
Fragments arranged by size:
5445 2081 47
StuI AGG'CCT
Cuts at: 1517 2213 4178 1517
Size: 696 1965 4912
Fragments arranged by size:
4912 1965 696
StyI C'CwwG_G
Cuts at: 513 1065 2058 2226 2785 3105 3916 3999 4173
Size: 552 993 168 559 320 811 83 174
Cuts at: 4173 5012 513
Size: 839 3074
Fragments arranged by size:
3074 993 839 811 559 552 320 174
168 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2284 4658 4853 5600 5617 5770 5955
Size: 1255 704 2374 195 747 17 153 185
Cuts at: 5955 6400 325
Size: 445 1498
Fragments arranged by size:
2374 1498 1255 747 704 445 195 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2845 5819 7531
Size: 274 80 33 51 1443 868 2974 1712
Cuts at: 7531 96
Size: 138
Fragments arranged by size:
2974 1712 1443 868 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 7533 7565 2006
Size: 32 5495 32 2014
Fragments arranged by size:
5495 2014 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7573
XbaI T'CTAG_A
Cuts at: 4909 4909
Size: 7573
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4787 4787
Size: 7573
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7573
XmaI C'CCGG_G
Cuts at: 1870 4898 1870
Size: 3028 4545
XmnI GAAnn'nnTTC
Cuts at: 5702 5702
Size: 7573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5381 276
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BfrBI BplI BsiWI BstZ17I
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI SapI SexAI SrfI SwaI