Restriction map for
pFN21AB8518
(Circular) (Six-base) MAPSORT of: pFN21AB8518.seq Check: 1309 from: 1 to: 7312
pFN21AB8518 7312 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4117 4117
Size: 7312
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3407 5122 278
Size: 53 83 186 936 1871 1715 2468
Fragments arranged by size:
2468 1871 1715 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4655 1937
Size: 2718 4594
Acc65I G'GTAC_C
Cuts at: 4633 4633
Size: 7312
AclI AA'CG_TT
Cuts at: 1563 5439 5812 1563
Size: 3876 373 3063
Fragments arranged by size:
3876 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2041 5375 6759 7027 1632
Size: 409 3334 1384 268 1917
Fragments arranged by size:
3334 1917 1384 409 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7115
AflIII A'CryG_T
Cuts at: 1203 6211 7188 1203
Size: 5008 977 1327
Fragments arranged by size:
5008 1327 977
AgeI A'CCGG_T
Cuts at: 6143 6143
Size: 7312
AhdI GACnn_n'nnGTC
Cuts at: 6041 6041
Size: 7312
AleI CACnn'nnGTG
Cuts at: 1166 3818 1166
Size: 2652 4660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7312
AlwNI CAG_nnn'CTG
Cuts at: 3113 4194 4352 5055 6627 3113
Size: 1081 158 703 1572 3798
Fragments arranged by size:
3798 1572 1081 703 158
ApaI G_GGCC'C
Cuts at: 4086 4086
Size: 7312
ApaLI G'TGCA_C
Cuts at: 5369 6525 5369
Size: 1156 6156
ApoI r'AATT_y
Cuts at: 1493 2199 2494 3899 4566 4621 4847 4999 7108
Size: 706 295 1405 667 55 226 152 2109
Cuts at: 7108 1493
Size: 1697
Fragments arranged by size:
2109 1697 1405 706 667 295 226 152
55
AseI AT'TA_AT
Cuts at: 160 1032 5866 160
Size: 872 4834 1606
Fragments arranged by size:
4834 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7312
AvaI C'yCGr_G
Cuts at: 1870 1943 2591 4637 1870
Size: 73 648 2046 4545
Fragments arranged by size:
4545 2046 648 73
BamHI G'GATC_C
Cuts at: 2643 4642 5009 2643
Size: 1999 367 4946
Fragments arranged by size:
4946 1999 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3784 4633 6088 618
Size: 325 217 627 1997 849 1455 1842
Fragments arranged by size:
1997 1842 1455 849 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 2347 2656 3095 3216 3633 4066 4086
Size: 625 331 309 439 121 417 433 20
Cuts at: 4086 4631 1391
Size: 545 4072
Fragments arranged by size:
4072 625 545 439 433 417 331 309
121 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 3257 928
Size: 971 381 977 4983
Fragments arranged by size:
4983 977 971 381
BbvCI CC'TCA_GC
Cuts at: 3114 3114
Size: 7312
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5503 5537 1903
Size: 34 3566 34 3678
Fragments arranged by size:
3678 3566 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3723 5204 6414 3723
Size: 1481 1210 4621
Fragments arranged by size:
4621 1481 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7312
BfrBI ATG'CAT
Cuts at: 2850 3283 3325 2850
Size: 433 42 6837
Fragments arranged by size:
6837 433 42
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3893 4203 5923
Size: 107 122 71 631 605 2221 310 1720
Cuts at: 5923 136
Size: 1525
Fragments arranged by size:
2221 1720 1525 631 605 310 122 107
71
BglII A'GATC_T
Cuts at: 4386 7307 4386
Size: 2921 4391
BlpI GC'TnA_GC
Cuts at: 3701 4104 4729 3701
Size: 403 625 6284
Fragments arranged by size:
6284 625 403
Bme1580I G_kGCm'C
Cuts at: 1790 3787 4086 5373 6529 1790
Size: 1997 299 1287 1156 2573
Fragments arranged by size:
2573 1997 1287 1156 299
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7312
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2953 3089 4539
Size: 416 53 83 399 24 1536 136 1450
Cuts at: 4539 5043 6001 442
Size: 504 958 1753
Fragments arranged by size:
1753 1536 1450 958 504 416 399 136
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4408 4408
Size: 7312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3177 3728 4057 4228 5972 1239
Size: 1938 551 329 171 1744 2579
Fragments arranged by size:
2579 1938 1744 551 329 171
Bpu10I CC'TnA_GC
Cuts at: 3114 4065 3114
Size: 951 6361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2548 2791 5437 6302 6600 2548
Size: 243 2646 865 298 3260
Fragments arranged by size:
3260 2646 865 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4075 5975 882
Size: 617 2576 1900 2219
Fragments arranged by size:
2576 2219 1900 617
BsaAI yAC'GTr
Cuts at: 493 1204 4447 5103 493
Size: 711 3243 656 2702
Fragments arranged by size:
3243 2702 711 656
BsaBI GATnn'nnATC
Cuts at: 7306 7306
Size: 7312
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3404 5119 5501 6136
Size: 53 83 186 936 1871 1715 382 635
Cuts at: 6136 275
Size: 1451
Fragments arranged by size:
1871 1715 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5745 6143 6417 6564 6975 5745
Size: 398 274 147 411 6082
Fragments arranged by size:
6082 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2313 2343 2365 2395 4451
Size: 30 165 30 910 30 22 30 2056
Cuts at: 4451 4481 1178
Size: 30 4009
Fragments arranged by size:
4009 2056 910 165 30 30 30 30
30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2091 2243 2337 3053 3436 3439 4445 1186
Size: 905 152 94 716 383 3 1006 4053
Fragments arranged by size:
4053 1006 905 716 383 152 94 3
BseYI C'CCAG_C
Cuts at: 2169 3317 6515 7011 2169
Size: 1148 3198 496 2470
Fragments arranged by size:
3198 2470 1148 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2172 2284 2681 3169 4051 1163
Size: 573 436 112 397 488 882 4424
Fragments arranged by size:
4424 882 573 488 436 397 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4762 5523 6551 1540
Size: 275 186 2761 761 1028 2301
Fragments arranged by size:
2761 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 3216 3633 5373 5458 6529 2016
Size: 1200 417 1740 85 1071 2799
Fragments arranged by size:
2799 1740 1200 1071 417 85
BsmI GAATG_Cn'
Cuts at: 2715 3308 4823 4916 2715
Size: 593 1515 93 5111
Fragments arranged by size:
5111 1515 593 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2347 2656 3095 3216 3633 3787
Size: 399 226 331 309 439 121 417 154
Cuts at: 3787 4066 4086 4631 5373 5458 6529 1391
Size: 279 20 545 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 439 417 399 331
309 279 226 154 121 85 20
BspEI T'CCGG_A
Cuts at: 6975 6975
Size: 7312
BspHI T'CATG_A
Cuts at: 5201 6931 5201
Size: 1730 5582
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4117 4667 844
Size: 3273 550 3489
Fragments arranged by size:
3489 3273 550
BsrBI CCG'CTC
Cuts at: 1394 1823 3679 5199 1394
Size: 429 1856 1520 3507
Fragments arranged by size:
3507 1856 1520 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4116 5807 5981 65
Size: 1183 2868 1691 174 1396
Fragments arranged by size:
2868 1691 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3102 5956 6143 1700
Size: 110 1292 2854 187 2869
Fragments arranged by size:
2869 2854 1292 187 110
BsrGI T'GTAC_A
Cuts at: 96 2702 4497 96
Size: 2606 1795 2911
Fragments arranged by size:
2911 2606 1795
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7312
BssSI C'ACGA_G
Cuts at: 5372 6384 5372
Size: 1012 6300
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2295 2999 794
Size: 1501 704 5107
Fragments arranged by size:
5107 1501 704
BstBI TT'CG_AA
Cuts at: 6116 6116
Size: 7312
BstEII G'GTnAC_C
Cuts at: 1184 3986 1184
Size: 2802 4510
BstXI CCAn_nnnn'nTGG
Cuts at: 4129 4129
Size: 7312
BstYI r'GATC_y
Cuts at: 1972 2643 3272 4386 4642 5009 5394 5411 6863
Size: 671 629 1114 256 367 385 17 1452
Cuts at: 6863 6949 6961 7307 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1114 671 629 385 367 346
256 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3036 3036
Size: 7312
BtgI C'CryG_G
Cuts at: 513 1065 3336 4488 513
Size: 552 2271 1152 3337
Fragments arranged by size:
3337 2271 1152 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6573
Fragments arranged by size:
6573 683 56
BtsI GCAGTG_nn'
Cuts at: 2160 3617 4828 5622 5642 2160
Size: 1457 1211 794 20 3830
Fragments arranged by size:
3830 1457 1211 794 20
ClaI AT'CG_AT
Cuts at: 1554 2569 1554
Size: 1015 6297
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2086 2121 529
Size: 35 1522 35 5720
Fragments arranged by size:
5720 1522 35 35
DraI TTT'AAA
Cuts at: 4616 4616
Size: 7312
DraIII CAC_nnn'GTG
Cuts at: 4027 4029 4027
Size: 2 7310
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6319 809
Size: 507 5003 1802
Fragments arranged by size:
5003 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2304 3104 4759
Size: 54 1425 50 275 18 474 800 1655
Cuts at: 4759 5648 7277 8
Size: 889 1629 43
Fragments arranged by size:
1655 1629 1425 889 800 474 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4759 1537
Size: 275 2947 4090
Fragments arranged by size:
4090 2947 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3221 3240 3457 5242 1353
Size: 1868 19 217 1785 3423
Fragments arranged by size:
3423 1868 1785 217 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5899 6273 6419 387
Size: 1064 138 4310 374 146 1280
Fragments arranged by size:
4310 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2014 3214 3631 2014
Size: 1200 417 5695
Fragments arranged by size:
5695 1200 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 3177 3728 4057 4228 5375 5972
Size: 393 409 1136 551 329 171 1147 597
Cuts at: 5972 6759 7027 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1147 1136 787 597 551 409 393
329 268 171
EcoO109I rG'GnC_Cy
Cuts at: 3331 4083 3331
Size: 752 6560
EcoRI G'AATT_C
Cuts at: 4566 4621 4999 4566
Size: 55 378 6879
Fragments arranged by size:
6879 378 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3020 3232 3020
Size: 212 7100
FspI TGC'GCA
Cuts at: 3223 5818 3223
Size: 2595 4717
HaeII r_GCGC'y
Cuts at: 1678 6459 1678
Size: 4781 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2001 2033 5953 5985 6027
Size: 32 135 32 429 32 3920 32 42
Cuts at: 6027 6059 1373
Size: 32 2626
Fragments arranged by size:
3920 2626 429 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2020 4656 4902 669
Size: 988 281 82 2636 246 3079
Fragments arranged by size:
3079 2636 988 281 246 82
HindIII A'AGCT_T
Cuts at: 748 3311 748
Size: 2563 4749
HpaI GTT'AAC
Cuts at: 4902 4902
Size: 7312
KpnI G_GTAC'C
Cuts at: 4637 4637
Size: 7312
MfeI C'AATT_G
Cuts at: 4911 4911
Size: 7312
MluI A'CGCG_T
Cuts at: 7188 7188
Size: 7312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4286 4499 6426 6610 1305
Size: 2981 213 1927 184 2007
Fragments arranged by size:
2981 2007 1927 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5490
Fragments arranged by size:
5490 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 3818 4217 5270 5629 5788 518
Size: 648 1224 1428 399 1053 359 159 2042
Fragments arranged by size:
2042 1428 1224 1053 648 399 359 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2818 3110 4136 4728 5405 6553 6798
Size: 455 757 292 1026 592 677 1148 245
Cuts at: 6798 1606
Size: 2120
Fragments arranged by size:
2120 1148 1026 757 677 592 455 292
245
NaeI GCC'GGC
Cuts at: 3104 3104
Size: 7312
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6760
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7312
NgoMIV G'CCGG_C
Cuts at: 3102 3102
Size: 7312
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7312
NotI GC'GGCC_GC
Cuts at: 4759 4759
Size: 7312
NsiI A_TGCA'T
Cuts at: 2852 3285 3327 2852
Size: 433 42 6837
Fragments arranged by size:
6837 433 42
NspI r_CATG'y
Cuts at: 1132 1229 2850 3325 4015 4582 4670 6215 1132
Size: 97 1621 475 690 567 88 1545 2229
Fragments arranged by size:
2229 1621 1545 690 567 475 97 88
PasI CC'CwG_GG
Cuts at: 1348 4079 1348
Size: 2731 4581
PciI A'CATG_T
Cuts at: 6211 6211
Size: 7312
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2736 1108
Size: 1628 5684
PfoI T'CCnGG_A
Cuts at: 2183 2235 3141 3154 4244 2183
Size: 52 906 13 1090 5251
Fragments arranged by size:
5251 1090 906 52 13
PmeI GTTT'AAAC
Cuts at: 4616 4616
Size: 7312
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5381 5413 6894 6926 4241
Size: 32 1108 32 1481 32 4627
Fragments arranged by size:
4627 1481 1108 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3331 3331
Size: 7312
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7312
PsiI TTA'TAA
Cuts at: 3369 4332 4882 3369
Size: 963 550 5799
Fragments arranged by size:
5799 963 550
PspOMI G'GGCC_C
Cuts at: 4082 4082
Size: 7312
PstI C_TGCA'G
Cuts at: 830 2817 2899 4407 4537 4664 830
Size: 1987 82 1508 130 127 3478
Fragments arranged by size:
3478 1987 1508 130 127 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7312
PvuII CAG'CTG
Cuts at: 2818 3110 4136 2818
Size: 292 1026 5994
Fragments arranged by size:
5994 1026 292
SacI G_AGCT'C
Cuts at: 2016 3216 3633 2016
Size: 1200 417 5695
Fragments arranged by size:
5695 1200 417
SalI G'TCGA_C
Cuts at: 1936 4654 1936
Size: 2718 4594
SanDI GG'GwC_CC
Cuts at: 3331 3331
Size: 7312
SapI GCTCTTCn'nnn_
Cuts at: 3221 3221
Size: 7312
SbfI CC_TGCA'GG
Cuts at: 4664 4664
Size: 7312
ScaI AGT'ACT
Cuts at: 2884 3194 5560 7272 2884
Size: 310 2366 1712 2924
Fragments arranged by size:
2924 2366 1712 310
SfcI C'TryA_G
Cuts at: 826 1045 2813 2895 3268 4344 4403 4533 4660
Size: 219 1768 82 373 1076 59 130 127
Cuts at: 4660 5795 6476 6667 826
Size: 1135 681 191 1471
Fragments arranged by size:
1768 1471 1135 1076 681 373 219 191
130 127 82 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7312
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7312
SmaI CCC'GGG
Cuts at: 1872 4639 1872
Size: 2767 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2527 2806 5416 6317 6579 820
Size: 197 926 584 279 2610 901 262 1553
Fragments arranged by size:
2610 1553 926 901 584 279 262 197
SnaBI TAC'GTA
Cuts at: 493 5103 493
Size: 4610 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7312
SphI G_CATG'C
Cuts at: 1132 3325 4670 1132
Size: 2193 1345 3774
Fragments arranged by size:
3774 2193 1345
SspI AAT'ATT
Cuts at: 5 52 3522 5236 5
Size: 47 3470 1714 2081
Fragments arranged by size:
3470 2081 1714 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7312
StyI C'CwwG_G
Cuts at: 513 1065 2889 3716 4751 513
Size: 552 1824 827 1035 3074
Fragments arranged by size:
3074 1824 1035 827 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2808 5339 5356 5509 5694 6139 325
Size: 1255 1228 2531 17 153 185 445 1498
Fragments arranged by size:
2531 1498 1255 1228 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2429 2702 2882
Size: 274 80 33 51 1443 452 273 180
Cuts at: 2882 2961 3192 4497 5558 7270 96
Size: 79 231 1305 1061 1712 138
Fragments arranged by size:
1712 1443 1305 1061 452 274 273 231
180 138 80 79 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2313 2345 4565 4597 7272 7304 2313
Size: 32 2220 32 2675 32 2321
Fragments arranged by size:
2675 2321 2220 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7312
XbaI T'CTAG_A
Cuts at: 3361 4648 3361
Size: 1287 6025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 2314 2460 4195 2266
Size: 48 146 1735 5383
Fragments arranged by size:
5383 1735 146 48
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7312
XmaI C'CCGG_G
Cuts at: 1870 4637 1870
Size: 2767 4545
XmnI GAAnn'nnTTC
Cuts at: 5441 5441
Size: 7312
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3405 5120 276
Size: 53 83 186 936 1871 1715 2468
Fragments arranged by size:
2468 1871 1715 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI