Restriction map for
pFN21AB8516
(Circular) (Six-base) MAPSORT of: pFN21AB8516.seq Check: 2991 from: 1 to: 7318
pFN21AB8516 7318 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4317 4317
Size: 7318
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5128 278
Size: 53 83 186 936 3592 2468
Fragments arranged by size:
3592 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3864 4661 1937
Size: 1927 797 4594
Fragments arranged by size:
4594 1927 797
Acc65I G'GTAC_C
Cuts at: 4480 4639 4480
Size: 159 7159
AclI AA'CG_TT
Cuts at: 1563 5445 5818 1563
Size: 3882 373 3063
Fragments arranged by size:
3882 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3812 3876 3985 4321 5381 6765 7033 1632
Size: 2180 64 109 336 1060 1384 268 1917
Fragments arranged by size:
2180 1917 1384 1060 336 268 109 64
AfeI AGC'GCT
Cuts at: 4241 4241
Size: 7318
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7121
AflIII A'CryG_T
Cuts at: 1203 3279 3281 6217 7194 1203
Size: 2076 2 2936 977 1327
Fragments arranged by size:
2936 2076 1327 977 2
AgeI A'CCGG_T
Cuts at: 6149 6149
Size: 7318
AhdI GACnn_n'nnGTC
Cuts at: 2476 6047 2476
Size: 3571 3747
AleI CACnn'nnGTG
Cuts at: 1166 2355 3518 4124 1166
Size: 1189 1163 606 4360
Fragments arranged by size:
4360 1189 1163 606
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2255 2990 1184
Size: 1071 735 5512
Fragments arranged by size:
5512 1071 735
AlwNI CAG_nnn'CTG
Cuts at: 3484 5061 6633 3484
Size: 1577 1572 4169
Fragments arranged by size:
4169 1577 1572
ApaI G_GGCC'C
Cuts at: 2040 2301 2040
Size: 261 7057
ApaLI G'TGCA_C
Cuts at: 5375 6531 5375
Size: 1156 6162
ApoI r'AATT_y
Cuts at: 1493 3625 4627 4853 5005 7114 1493
Size: 2132 1002 226 152 2109 1697
Fragments arranged by size:
2132 2109 1697 1002 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5872 160
Size: 872 4840 1606
Fragments arranged by size:
4840 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7318
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2060 2481 4399 4643 1870
Size: 73 96 21 421 1918 244 4545
Fragments arranged by size:
4545 1918 421 244 96 73 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3485 3518 3485
Size: 33 7285
BamHI G'GATC_C
Cuts at: 4648 5015 4648
Size: 367 6951
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2222 2239 2290 2336
Size: 325 217 627 288 147 17 51 46
Cuts at: 2336 2949 3204 4480 4531 4639 6094 618
Size: 613 255 1276 51 108 1455 1842
Fragments arranged by size:
1842 1455 1276 627 613 325 288 255
217 147 108 51 51 46 17
BanII G_rGCy'C
Cuts at: 1391 2040 2058 2301 2408 2489 3366 4307 4637
Size: 649 18 243 107 81 877 941 330
Cuts at: 4637 1391
Size: 4072
Fragments arranged by size:
4072 941 877 649 330 243 107 81
18
BbeI G_GCGC'C
Cuts at: 2226 2953 2226
Size: 727 6591
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2440 2585 4078 928
Size: 971 541 145 1493 4168
Fragments arranged by size:
4168 1493 971 541 145
BbvCI CC'TCA_GC
Cuts at: 2424 2424
Size: 7318
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5509 5543 1903
Size: 34 3572 34 3678
Fragments arranged by size:
3678 3572 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5210 6420 5210
Size: 1210 6108
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7318
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2178 3728 5929
Size: 107 122 71 631 605 506 1550 2201
Cuts at: 5929 136
Size: 1525
Fragments arranged by size:
2201 1550 1525 631 605 506 122 107
71
BglII A'GATC_T
Cuts at: 7313 7313
Size: 7318
BlpI GC'TnA_GC
Cuts at: 2052 4061 4735 2052
Size: 2009 674 4635
Fragments arranged by size:
4635 2009 674
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2244 2301 3067 3494 3969 5379 6535
Size: 250 204 57 766 427 475 1410 1156
Cuts at: 6535 1790
Size: 2573
Fragments arranged by size:
2573 1410 1156 766 475 427 250 204
57
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3238 3573 5049
Size: 416 53 83 399 24 1821 335 1476
Cuts at: 5049 6007 442
Size: 958 1753
Fragments arranged by size:
1821 1753 1476 958 416 399 335 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7318
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 7318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 2338 3110 3326 3506 3639 4073 5978
Size: 1043 56 772 216 180 133 434 1905
Cuts at: 5978 1239
Size: 2579
Fragments arranged by size:
2579 1905 1043 772 434 216 180 133
56
Bpu10I CC'TnA_GC
Cuts at: 2424 2609 2424
Size: 185 7133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3041 5443 6308 6606 3041
Size: 2402 865 298 3753
Fragments arranged by size:
3753 2402 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3436 3907 3989 4183 4365 4406 5981
Size: 617 1937 471 82 194 182 41 1575
Cuts at: 5981 882
Size: 2219
Fragments arranged by size:
2219 1937 1575 617 471 194 182 82
41
BsaAI yAC'GTr
Cuts at: 493 1204 2764 3282 5109 493
Size: 711 1560 518 1827 2702
Fragments arranged by size:
2702 1827 1560 711 518
BsaBI GATnn'nnATC
Cuts at: 3155 7312 3155
Size: 4157 3161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 2950 4608 5125
Size: 53 83 186 936 690 727 1658 517
Cuts at: 5125 5507 6142 275
Size: 382 635 1451
Fragments arranged by size:
1658 1451 936 727 690 635 517 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5751 6149 6423 6570 6981 5751
Size: 398 274 147 411 6088
Fragments arranged by size:
6088 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2778 2808 3254 3284 3416
Size: 30 165 30 1375 30 446 30 132
Cuts at: 3416 3446 1178
Size: 30 5050
Fragments arranged by size:
5050 1375 446 165 132 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 2567 3259 4124 1186
Size: 909 472 692 865 4380
Fragments arranged by size:
4380 909 865 692 472
BseYI C'CCAG_C
Cuts at: 6521 7017 6521
Size: 496 6822
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 2869 3943 1163
Size: 573 978 155 1074 4538
Fragments arranged by size:
4538 1074 978 573 155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4768 5529 6557 1540
Size: 275 186 2767 761 1028 2301
Fragments arranged by size:
2767 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 3102 4307 5379 5464 6535 2058
Size: 1044 1205 1072 85 1071 2841
Fragments arranged by size:
2841 1205 1072 1071 1044 85
BsmI GAATG_Cn'
Cuts at: 3772 4829 4922 3772
Size: 1057 93 6168
Fragments arranged by size:
6168 1057 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3415 3694 3415
Size: 279 7039
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2058 2244 2301 2408 2489 3067
Size: 399 250 18 186 57 107 81 578
Cuts at: 3067 3102 3366 3494 3969 4307 4637 5379 5464
Size: 35 264 128 475 338 330 742 85
Cuts at: 5464 6535 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 578 475 399 338 330
264 250 186 128 107 85 81 57
35 18
BspEI T'CCGG_A
Cuts at: 6981 6981
Size: 7318
BspHI T'CATG_A
Cuts at: 3126 3603 3816 3876 5207 6937 3126
Size: 477 213 60 1331 1730 3507
Fragments arranged by size:
3507 1730 1331 477 213 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2591 3054 3537 4317 4673 844
Size: 1747 463 483 780 356 3489
Fragments arranged by size:
3489 1747 780 483 463 356
BsrBI CCG'CTC
Cuts at: 1394 1823 4275 4615 5205 1394
Size: 429 2452 340 590 3507
Fragments arranged by size:
3507 2452 590 429 340
BsrDI GCAATG_nn'
Cuts at: 65 1248 2620 2994 3946 4146 5813 5987 65
Size: 1183 1372 374 952 200 1667 174 1396
Fragments arranged by size:
1667 1396 1372 1183 952 374 200 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2155 2454 2663 5962 6149 1700
Size: 110 345 299 209 3299 187 2869
Fragments arranged by size:
3299 2869 345 299 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 3318 96
Size: 3222 4096
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7318
BssSI C'ACGA_G
Cuts at: 2774 3344 3979 5378 6390 2774
Size: 570 635 1399 1012 3702
Fragments arranged by size:
3702 1399 1012 635 570
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4332 794
Size: 3538 3780
BstBI TT'CG_AA
Cuts at: 6122 6122
Size: 7318
BstEII G'GTnAC_C
Cuts at: 1184 4102 4288 4580 1184
Size: 2918 186 292 3922
Fragments arranged by size:
3922 2918 292 186
BstXI CCAn_nnnn'nTGG
Cuts at: 2810 4042 4228 4427 2810
Size: 1232 186 199 5701
Fragments arranged by size:
5701 1232 199 186
BstYI r'GATC_y
Cuts at: 1972 3156 3309 4648 5015 5400 5417 6869 6955
Size: 1184 153 1339 367 385 17 1452 86
Cuts at: 6955 6967 7313 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1339 1184 385 367 346 153
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4259 4259
Size: 7318
BtgI C'CryG_G
Cuts at: 513 1065 2004 2313 2809 3447 3516 3784 4080
Size: 552 939 309 496 638 69 268 296
Cuts at: 4080 4293 4443 513
Size: 213 150 3388
Fragments arranged by size:
3388 939 638 552 496 309 296 268
213 150 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2202 2635 2719 4578 487
Size: 683 56 976 433 84 1859 3227
Fragments arranged by size:
3227 1859 976 683 433 84 56
BtsI GCAGTG_nn'
Cuts at: 2490 4217 4834 5628 5648 2490
Size: 1727 617 794 20 4160
Fragments arranged by size:
4160 1727 794 617 20
ClaI AT'CG_AT
Cuts at: 1554 2253 1554
Size: 699 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7283
DraI TTT'AAA
Cuts at: 4622 4622
Size: 7318
DraIII CAC_nnn'GTG
Cuts at: 2764 2764
Size: 7318
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6325 809
Size: 507 5009 1802
Fragments arranged by size:
5009 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2617 2991
Size: 54 1425 50 275 18 282 505 374
Cuts at: 2991 3729 4567 4765 5654 7283 8
Size: 738 838 198 889 1629 43
Fragments arranged by size:
1629 1425 889 838 738 505 374 282
275 198 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4765 1537
Size: 275 2953 4090
Fragments arranged by size:
4090 2953 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3077 3285 3563 3638 3839 3878 5248 1353
Size: 1724 208 278 75 201 39 1370 3423
Fragments arranged by size:
3423 1724 1370 278 208 201 75 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2954 5905 6279 6425 387
Size: 1064 138 435 930 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 930 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2056 4305 2056
Size: 2249 5069
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2338 3110 3326 3506 3639 3812
Size: 393 650 56 772 216 180 133 173
Cuts at: 3812 3876 3985 4073 4321 5381 5978 6765 7033
Size: 64 109 88 248 1060 597 787 268
Cuts at: 7033 1239
Size: 1524
Fragments arranged by size:
1524 1060 787 772 650 597 393 268
248 216 180 173 133 109 88 64
56
EcoNI CCTnn'n_nnAGG
Cuts at: 2591 4262 2591
Size: 1671 5647
EcoO109I rG'GnC_Cy
Cuts at: 2024 2036 3568 3804 3841 4396 2024
Size: 12 1532 236 37 555 4946
Fragments arranged by size:
4946 1532 555 236 37 12
EcoRI G'AATT_C
Cuts at: 3625 4627 5005 3625
Size: 1002 378 5938
Fragments arranged by size:
5938 1002 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3850 3850
Size: 7318
FspI TGC'GCA
Cuts at: 2207 5824 2207
Size: 3617 3701
HaeII r_GCGC'y
Cuts at: 1678 2226 2818 2953 4243 6465 1678
Size: 548 592 135 1290 2222 2531
Fragments arranged by size:
2531 2222 1290 592 548 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 2778 2810 3101
Size: 32 135 32 890 32 284 32 291
Cuts at: 3101 3133 3254 3286 3843 3875 4454 4486 5959
Size: 32 121 32 557 32 579 32 1473
Cuts at: 5959 5991 6033 6065 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1473 890 579 557 291 284 135
121 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2908 4662 4908 669
Size: 988 281 970 1754 246 3079
Fragments arranged by size:
3079 1754 988 970 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7318
HpaI GTT'AAC
Cuts at: 4908 4908
Size: 7318
KasI G'GCGC_C
Cuts at: 2222 2949 2222
Size: 727 6591
KpnI G_GTAC'C
Cuts at: 4484 4643 4484
Size: 159 7159
MfeI C'AATT_G
Cuts at: 4917 4917
Size: 7318
MluI A'CGCG_T
Cuts at: 7194 7194
Size: 7318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6432 6616 1305
Size: 5127 184 2007
Fragments arranged by size:
5127 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 2993 4569 10
Size: 54 1425 343 282 879 1576 2759
Fragments arranged by size:
2759 1576 1425 879 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2355 2636 3425 3518 3932 4040 4124
Size: 648 1189 281 789 93 414 108 84
Cuts at: 4124 5276 5635 5794 518
Size: 1152 359 159 2042
Fragments arranged by size:
2042 1189 1152 789 648 414 359 281
159 108 93 84
MspA1I CmG'CkG
Cuts at: 1606 3484 3655 4560 4734 5411 6559 6804 1606
Size: 1878 171 905 174 677 1148 245 2120
Fragments arranged by size:
2120 1878 1148 905 677 245 174 171
NarI GG'CG_CC
Cuts at: 2223 2950 2223
Size: 727 6591
NcoI C'CATG_G
Cuts at: 513 1065 2004 2313 3447 513
Size: 552 939 309 1134 4384
Fragments arranged by size:
4384 1134 939 552 309
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7318
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7318
NotI GC'GGCC_GC
Cuts at: 4765 4765
Size: 7318
NruI TCG'CGA
Cuts at: 2962 2962
Size: 7318
NspI r_CATG'y
Cuts at: 1132 1229 2947 2984 4144 4676 6221 1132
Size: 97 1718 37 1160 532 1545 2229
Fragments arranged by size:
2229 1718 1545 1160 532 97 37
PasI CC'CwG_GG
Cuts at: 1348 2782 1348
Size: 1434 5884
PciI A'CATG_T
Cuts at: 6217 6217
Size: 7318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2189 3484 4378 1108
Size: 1081 1295 894 4048
Fragments arranged by size:
4048 1295 1081 894
PfoI T'CCnGG_A
Cuts at: 4014 4227 4014
Size: 213 7105
PmeI GTTT'AAAC
Cuts at: 4622 4622
Size: 7318
PmlI CAC'GTG
Cuts at: 3282 3282
Size: 7318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 5387 5419 6900 6932 4493
Size: 32 862 32 1481 32 4879
Fragments arranged by size:
4879 1481 862 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3568 3804 3841 4396 3568
Size: 236 37 555 6490
Fragments arranged by size:
6490 555 236 37
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7318
PsiI TTA'TAA
Cuts at: 4888 4888
Size: 7318
PspOMI G'GGCC_C
Cuts at: 2036 2297 2036
Size: 261 7057
PstI C_TGCA'G
Cuts at: 830 4212 4670 830
Size: 3382 458 3478
Fragments arranged by size:
3478 3382 458
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7318
PvuII CAG'CTG
Cuts at: 3655 3655
Size: 7318
SacI G_AGCT'C
Cuts at: 2058 4307 2058
Size: 2249 5069
SalI G'TCGA_C
Cuts at: 1936 4660 1936
Size: 2724 4594
SapI GCTCTTCn'nnn_
Cuts at: 3563 3878 3563
Size: 315 7003
SbfI CC_TGCA'GG
Cuts at: 4670 4670
Size: 7318
ScaI AGT'ACT
Cuts at: 5566 7278 5566
Size: 1712 5606
SfcI C'TryA_G
Cuts at: 826 1045 3808 4208 4666 5801 6482 6673 826
Size: 219 2763 400 458 1135 681 191 1471
Fragments arranged by size:
2763 1471 1135 681 458 400 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3728 3728
Size: 7318
SfoI GGC'GCC
Cuts at: 2224 2951 2224
Size: 727 6591
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7318
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7318
SmaI CCC'GGG
Cuts at: 1872 2041 2062 2483 4401 4645 1872
Size: 169 21 421 1918 244 4545
Fragments arranged by size:
4545 1918 421 244 169 21
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3056 5422 6323 6585 820
Size: 197 926 1113 2366 901 262 1553
Fragments arranged by size:
2366 1553 1113 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5109 493
Size: 4616 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7318
SphI G_CATG'C
Cuts at: 1132 2984 4676 1132
Size: 1852 1692 3774
Fragments arranged by size:
3774 1852 1692
SspI AAT'ATT
Cuts at: 5 52 5242 5
Size: 47 5190 2081
Fragments arranged by size:
5190 2081 47
StuI AGG'CCT
Cuts at: 1517 2597 3296 3722 4268 1517
Size: 1080 699 426 546 4567
Fragments arranged by size:
4567 1080 699 546 426
StyI C'CwwG_G
Cuts at: 513 1065 2004 2313 2592 2829 3447 4182 4757
Size: 552 939 309 279 237 618 735 575
Cuts at: 4757 513
Size: 3074
Fragments arranged by size:
3074 939 735 618 575 552 309 279
237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2326 5345 5362 5515 5700 6145 325
Size: 1255 746 3019 17 153 185 445 1498
Fragments arranged by size:
3019 1498 1255 746 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3318 5564 7276
Size: 274 80 33 51 1443 1341 2246 1712
Cuts at: 7276 96
Size: 138
Fragments arranged by size:
2246 1712 1443 1341 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7278 7310 7278
Size: 32 7286
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7318
XbaI T'CTAG_A
Cuts at: 4654 4654
Size: 7318
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2497 4078 4225 2257
Size: 240 1581 147 5350
Fragments arranged by size:
5350 1581 240 147
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7318
XmaI C'CCGG_G
Cuts at: 1870 2039 2060 2481 4399 4643 1870
Size: 169 21 421 1918 244 4545
Fragments arranged by size:
4545 1918 421 244 169 21
XmnI GAAnn'nnTTC
Cuts at: 4268 5447 4268
Size: 1179 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5126 276
Size: 53 83 186 936 3592 2468
Fragments arranged by size:
3592 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FseI
FspAI NaeI NgoMIV NsiI PacI PspXI PsrI RsrII
SacII SanDI SexAI SrfI SwaI