(Circular) (Six-base) MAPSORT of: pFN21AB8516.seq Check: 2991 from: 1 to: 7318 pFN21AB8516 7318 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4317 4317 Size: 7318 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5128 278 Size: 53 83 186 936 3592 2468 Fragments arranged by size: 3592 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3864 4661 1937 Size: 1927 797 4594 Fragments arranged by size: 4594 1927 797 Acc65I G'GTAC_C Cuts at: 4480 4639 4480 Size: 159 7159 AclI AA'CG_TT Cuts at: 1563 5445 5818 1563 Size: 3882 373 3063 Fragments arranged by size: 3882 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3812 3876 3985 4321 5381 6765 7033 1632 Size: 2180 64 109 336 1060 1384 268 1917 Fragments arranged by size: 2180 1917 1384 1060 336 268 109 64 AfeI AGC'GCT Cuts at: 4241 4241 Size: 7318 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7121 AflIII A'CryG_T Cuts at: 1203 3279 3281 6217 7194 1203 Size: 2076 2 2936 977 1327 Fragments arranged by size: 2936 2076 1327 977 2 AgeI A'CCGG_T Cuts at: 6149 6149 Size: 7318 AhdI GACnn_n'nnGTC Cuts at: 2476 6047 2476 Size: 3571 3747 AleI CACnn'nnGTG Cuts at: 1166 2355 3518 4124 1166 Size: 1189 1163 606 4360 Fragments arranged by size: 4360 1189 1163 606 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2255 2990 1184 Size: 1071 735 5512 Fragments arranged by size: 5512 1071 735 AlwNI CAG_nnn'CTG Cuts at: 3484 5061 6633 3484 Size: 1577 1572 4169 Fragments arranged by size: 4169 1577 1572 ApaI G_GGCC'C Cuts at: 2040 2301 2040 Size: 261 7057 ApaLI G'TGCA_C Cuts at: 5375 6531 5375 Size: 1156 6162 ApoI r'AATT_y Cuts at: 1493 3625 4627 4853 5005 7114 1493 Size: 2132 1002 226 152 2109 1697 Fragments arranged by size: 2132 2109 1697 1002 226 152 AseI AT'TA_AT Cuts at: 160 1032 5872 160 Size: 872 4840 1606 Fragments arranged by size: 4840 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7318 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2060 2481 4399 4643 1870 Size: 73 96 21 421 1918 244 4545 Fragments arranged by size: 4545 1918 421 244 96 73 21 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3485 3518 3485 Size: 33 7285 BamHI G'GATC_C Cuts at: 4648 5015 4648 Size: 367 6951 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2222 2239 2290 2336 Size: 325 217 627 288 147 17 51 46 Cuts at: 2336 2949 3204 4480 4531 4639 6094 618 Size: 613 255 1276 51 108 1455 1842 Fragments arranged by size: 1842 1455 1276 627 613 325 288 255 217 147 108 51 51 46 17 BanII G_rGCy'C Cuts at: 1391 2040 2058 2301 2408 2489 3366 4307 4637 Size: 649 18 243 107 81 877 941 330 Cuts at: 4637 1391 Size: 4072 Fragments arranged by size: 4072 941 877 649 330 243 107 81 18 BbeI G_GCGC'C Cuts at: 2226 2953 2226 Size: 727 6591 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2440 2585 4078 928 Size: 971 541 145 1493 4168 Fragments arranged by size: 4168 1493 971 541 145 BbvCI CC'TCA_GC Cuts at: 2424 2424 Size: 7318 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5509 5543 1903 Size: 34 3572 34 3678 Fragments arranged by size: 3678 3572 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5210 6420 5210 Size: 1210 6108 BclI T'GATC_A Cuts at: 1548 1548 Size: 7318 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2178 3728 5929 Size: 107 122 71 631 605 506 1550 2201 Cuts at: 5929 136 Size: 1525 Fragments arranged by size: 2201 1550 1525 631 605 506 122 107 71 BglII A'GATC_T Cuts at: 7313 7313 Size: 7318 BlpI GC'TnA_GC Cuts at: 2052 4061 4735 2052 Size: 2009 674 4635 Fragments arranged by size: 4635 2009 674 Bme1580I G_kGCm'C Cuts at: 1790 2040 2244 2301 3067 3494 3969 5379 6535 Size: 250 204 57 766 427 475 1410 1156 Cuts at: 6535 1790 Size: 2573 Fragments arranged by size: 2573 1410 1156 766 475 427 250 204 57 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7318 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3238 3573 5049 Size: 416 53 83 399 24 1821 335 1476 Cuts at: 5049 6007 442 Size: 958 1753 Fragments arranged by size: 1821 1753 1476 958 416 399 335 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7318 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2155 2155 Size: 7318 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 2338 3110 3326 3506 3639 4073 5978 Size: 1043 56 772 216 180 133 434 1905 Cuts at: 5978 1239 Size: 2579 Fragments arranged by size: 2579 1905 1043 772 434 216 180 133 56 Bpu10I CC'TnA_GC Cuts at: 2424 2609 2424 Size: 185 7133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3041 5443 6308 6606 3041 Size: 2402 865 298 3753 Fragments arranged by size: 3753 2402 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3436 3907 3989 4183 4365 4406 5981 Size: 617 1937 471 82 194 182 41 1575 Cuts at: 5981 882 Size: 2219 Fragments arranged by size: 2219 1937 1575 617 471 194 182 82 41 BsaAI yAC'GTr Cuts at: 493 1204 2764 3282 5109 493 Size: 711 1560 518 1827 2702 Fragments arranged by size: 2702 1827 1560 711 518 BsaBI GATnn'nnATC Cuts at: 3155 7312 3155 Size: 4157 3161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2223 2950 4608 5125 Size: 53 83 186 936 690 727 1658 517 Cuts at: 5125 5507 6142 275 Size: 382 635 1451 Fragments arranged by size: 1658 1451 936 727 690 635 517 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5751 6149 6423 6570 6981 5751 Size: 398 274 147 411 6088 Fragments arranged by size: 6088 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2778 2808 3254 3284 3416 Size: 30 165 30 1375 30 446 30 132 Cuts at: 3416 3446 1178 Size: 30 5050 Fragments arranged by size: 5050 1375 446 165 132 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2095 2567 3259 4124 1186 Size: 909 472 692 865 4380 Fragments arranged by size: 4380 909 865 692 472 BseYI C'CCAG_C Cuts at: 6521 7017 6521 Size: 496 6822 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2714 2869 3943 1163 Size: 573 978 155 1074 4538 Fragments arranged by size: 4538 1074 978 573 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4768 5529 6557 1540 Size: 275 186 2767 761 1028 2301 Fragments arranged by size: 2767 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2058 3102 4307 5379 5464 6535 2058 Size: 1044 1205 1072 85 1071 2841 Fragments arranged by size: 2841 1205 1072 1071 1044 85 BsmI GAATG_Cn' Cuts at: 3772 4829 4922 3772 Size: 1057 93 6168 Fragments arranged by size: 6168 1057 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3415 3694 3415 Size: 279 7039 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2058 2244 2301 2408 2489 3067 Size: 399 250 18 186 57 107 81 578 Cuts at: 3067 3102 3366 3494 3969 4307 4637 5379 5464 Size: 35 264 128 475 338 330 742 85 Cuts at: 5464 6535 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 578 475 399 338 330 264 250 186 128 107 85 81 57 35 18 BspEI T'CCGG_A Cuts at: 6981 6981 Size: 7318 BspHI T'CATG_A Cuts at: 3126 3603 3816 3876 5207 6937 3126 Size: 477 213 60 1331 1730 3507 Fragments arranged by size: 3507 1730 1331 477 213 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2591 3054 3537 4317 4673 844 Size: 1747 463 483 780 356 3489 Fragments arranged by size: 3489 1747 780 483 463 356 BsrBI CCG'CTC Cuts at: 1394 1823 4275 4615 5205 1394 Size: 429 2452 340 590 3507 Fragments arranged by size: 3507 2452 590 429 340 BsrDI GCAATG_nn' Cuts at: 65 1248 2620 2994 3946 4146 5813 5987 65 Size: 1183 1372 374 952 200 1667 174 1396 Fragments arranged by size: 1667 1396 1372 1183 952 374 200 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2155 2454 2663 5962 6149 1700 Size: 110 345 299 209 3299 187 2869 Fragments arranged by size: 3299 2869 345 299 209 187 110 BsrGI T'GTAC_A Cuts at: 96 3318 96 Size: 3222 4096 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7318 BssSI C'ACGA_G Cuts at: 2774 3344 3979 5378 6390 2774 Size: 570 635 1399 1012 3702 Fragments arranged by size: 3702 1399 1012 635 570 BstAPI GCAn_nnn'nTGC Cuts at: 794 4332 794 Size: 3538 3780 BstBI TT'CG_AA Cuts at: 6122 6122 Size: 7318 BstEII G'GTnAC_C Cuts at: 1184 4102 4288 4580 1184 Size: 2918 186 292 3922 Fragments arranged by size: 3922 2918 292 186 BstXI CCAn_nnnn'nTGG Cuts at: 2810 4042 4228 4427 2810 Size: 1232 186 199 5701 Fragments arranged by size: 5701 1232 199 186 BstYI r'GATC_y Cuts at: 1972 3156 3309 4648 5015 5400 5417 6869 6955 Size: 1184 153 1339 367 385 17 1452 86 Cuts at: 6955 6967 7313 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1339 1184 385 367 346 153 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4259 4259 Size: 7318 BtgI C'CryG_G Cuts at: 513 1065 2004 2313 2809 3447 3516 3784 4080 Size: 552 939 309 496 638 69 268 296 Cuts at: 4080 4293 4443 513 Size: 213 150 3388 Fragments arranged by size: 3388 939 638 552 496 309 296 268 213 150 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2202 2635 2719 4578 487 Size: 683 56 976 433 84 1859 3227 Fragments arranged by size: 3227 1859 976 683 433 84 56 BtsI GCAGTG_nn' Cuts at: 2490 4217 4834 5628 5648 2490 Size: 1727 617 794 20 4160 Fragments arranged by size: 4160 1727 794 617 20 ClaI AT'CG_AT Cuts at: 1554 2253 1554 Size: 699 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7283 DraI TTT'AAA Cuts at: 4622 4622 Size: 7318 DraIII CAC_nnn'GTG Cuts at: 2764 2764 Size: 7318 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6325 809 Size: 507 5009 1802 Fragments arranged by size: 5009 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 2617 2991 Size: 54 1425 50 275 18 282 505 374 Cuts at: 2991 3729 4567 4765 5654 7283 8 Size: 738 838 198 889 1629 43 Fragments arranged by size: 1629 1425 889 838 738 505 374 282 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4765 1537 Size: 275 2953 4090 Fragments arranged by size: 4090 2953 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3077 3285 3563 3638 3839 3878 5248 1353 Size: 1724 208 278 75 201 39 1370 3423 Fragments arranged by size: 3423 1724 1370 278 208 201 75 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2954 5905 6279 6425 387 Size: 1064 138 435 930 2951 374 146 1280 Fragments arranged by size: 2951 1280 1064 930 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2056 4305 2056 Size: 2249 5069 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 2338 3110 3326 3506 3639 3812 Size: 393 650 56 772 216 180 133 173 Cuts at: 3812 3876 3985 4073 4321 5381 5978 6765 7033 Size: 64 109 88 248 1060 597 787 268 Cuts at: 7033 1239 Size: 1524 Fragments arranged by size: 1524 1060 787 772 650 597 393 268 248 216 180 173 133 109 88 64 56 EcoNI CCTnn'n_nnAGG Cuts at: 2591 4262 2591 Size: 1671 5647 EcoO109I rG'GnC_Cy Cuts at: 2024 2036 3568 3804 3841 4396 2024 Size: 12 1532 236 37 555 4946 Fragments arranged by size: 4946 1532 555 236 37 12 EcoRI G'AATT_C Cuts at: 3625 4627 5005 3625 Size: 1002 378 5938 Fragments arranged by size: 5938 1002 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3850 3850 Size: 7318 FspI TGC'GCA Cuts at: 2207 5824 2207 Size: 3617 3701 HaeII r_GCGC'y Cuts at: 1678 2226 2818 2953 4243 6465 1678 Size: 548 592 135 1290 2222 2531 Fragments arranged by size: 2531 2222 1290 592 548 135 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 2778 2810 3101 Size: 32 135 32 890 32 284 32 291 Cuts at: 3101 3133 3254 3286 3843 3875 4454 4486 5959 Size: 32 121 32 557 32 579 32 1473 Cuts at: 5959 5991 6033 6065 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1473 890 579 557 291 284 135 121 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2908 4662 4908 669 Size: 988 281 970 1754 246 3079 Fragments arranged by size: 3079 1754 988 970 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7318 HpaI GTT'AAC Cuts at: 4908 4908 Size: 7318 KasI G'GCGC_C Cuts at: 2222 2949 2222 Size: 727 6591 KpnI G_GTAC'C Cuts at: 4484 4643 4484 Size: 159 7159 MfeI C'AATT_G Cuts at: 4917 4917 Size: 7318 MluI A'CGCG_T Cuts at: 7194 7194 Size: 7318 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6432 6616 1305 Size: 5127 184 2007 Fragments arranged by size: 5127 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2114 2993 4569 10 Size: 54 1425 343 282 879 1576 2759 Fragments arranged by size: 2759 1576 1425 879 343 282 54 MslI CAynn'nnrTG Cuts at: 518 1166 2355 2636 3425 3518 3932 4040 4124 Size: 648 1189 281 789 93 414 108 84 Cuts at: 4124 5276 5635 5794 518 Size: 1152 359 159 2042 Fragments arranged by size: 2042 1189 1152 789 648 414 359 281 159 108 93 84 MspA1I CmG'CkG Cuts at: 1606 3484 3655 4560 4734 5411 6559 6804 1606 Size: 1878 171 905 174 677 1148 245 2120 Fragments arranged by size: 2120 1878 1148 905 677 245 174 171 NarI GG'CG_CC Cuts at: 2223 2950 2223 Size: 727 6591 NcoI C'CATG_G Cuts at: 513 1065 2004 2313 3447 513 Size: 552 939 309 1134 4384 Fragments arranged by size: 4384 1134 939 552 309 NdeI CA'TA_TG Cuts at: 387 387 Size: 7318 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7318 NotI GC'GGCC_GC Cuts at: 4765 4765 Size: 7318 NruI TCG'CGA Cuts at: 2962 2962 Size: 7318 NspI r_CATG'y Cuts at: 1132 1229 2947 2984 4144 4676 6221 1132 Size: 97 1718 37 1160 532 1545 2229 Fragments arranged by size: 2229 1718 1545 1160 532 97 37 PasI CC'CwG_GG Cuts at: 1348 2782 1348 Size: 1434 5884 PciI A'CATG_T Cuts at: 6217 6217 Size: 7318 PflMI CCAn_nnn'nTGG Cuts at: 1108 2189 3484 4378 1108 Size: 1081 1295 894 4048 Fragments arranged by size: 4048 1295 1081 894 PfoI T'CCnGG_A Cuts at: 4014 4227 4014 Size: 213 7105 PmeI GTTT'AAAC Cuts at: 4622 4622 Size: 7318 PmlI CAC'GTG Cuts at: 3282 3282 Size: 7318 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4493 4525 5387 5419 6900 6932 4493 Size: 32 862 32 1481 32 4879 Fragments arranged by size: 4879 1481 862 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3568 3804 3841 4396 3568 Size: 236 37 555 6490 Fragments arranged by size: 6490 555 236 37 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7318 PsiI TTA'TAA Cuts at: 4888 4888 Size: 7318 PspOMI G'GGCC_C Cuts at: 2036 2297 2036 Size: 261 7057 PstI C_TGCA'G Cuts at: 830 4212 4670 830 Size: 3382 458 3478 Fragments arranged by size: 3478 3382 458 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7318 PvuII CAG'CTG Cuts at: 3655 3655 Size: 7318 SacI G_AGCT'C Cuts at: 2058 4307 2058 Size: 2249 5069 SalI G'TCGA_C Cuts at: 1936 4660 1936 Size: 2724 4594 SapI GCTCTTCn'nnn_ Cuts at: 3563 3878 3563 Size: 315 7003 SbfI CC_TGCA'GG Cuts at: 4670 4670 Size: 7318 ScaI AGT'ACT Cuts at: 5566 7278 5566 Size: 1712 5606 SfcI C'TryA_G Cuts at: 826 1045 3808 4208 4666 5801 6482 6673 826 Size: 219 2763 400 458 1135 681 191 1471 Fragments arranged by size: 2763 1471 1135 681 458 400 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3728 3728 Size: 7318 SfoI GGC'GCC Cuts at: 2224 2951 2224 Size: 727 6591 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7318 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7318 SmaI CCC'GGG Cuts at: 1872 2041 2062 2483 4401 4645 1872 Size: 169 21 421 1918 244 4545 Fragments arranged by size: 4545 1918 421 244 169 21 SmlI C'TyrA_G Cuts at: 820 1017 1943 3056 5422 6323 6585 820 Size: 197 926 1113 2366 901 262 1553 Fragments arranged by size: 2366 1553 1113 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5109 493 Size: 4616 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7318 SphI G_CATG'C Cuts at: 1132 2984 4676 1132 Size: 1852 1692 3774 Fragments arranged by size: 3774 1852 1692 SspI AAT'ATT Cuts at: 5 52 5242 5 Size: 47 5190 2081 Fragments arranged by size: 5190 2081 47 StuI AGG'CCT Cuts at: 1517 2597 3296 3722 4268 1517 Size: 1080 699 426 546 4567 Fragments arranged by size: 4567 1080 699 546 426 StyI C'CwwG_G Cuts at: 513 1065 2004 2313 2592 2829 3447 4182 4757 Size: 552 939 309 279 237 618 735 575 Cuts at: 4757 513 Size: 3074 Fragments arranged by size: 3074 939 735 618 575 552 309 279 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2326 5345 5362 5515 5700 6145 325 Size: 1255 746 3019 17 153 185 445 1498 Fragments arranged by size: 3019 1498 1255 746 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3318 5564 7276 Size: 274 80 33 51 1443 1341 2246 1712 Cuts at: 7276 96 Size: 138 Fragments arranged by size: 2246 1712 1443 1341 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7278 7310 7278 Size: 32 7286 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7318 XbaI T'CTAG_A Cuts at: 4654 4654 Size: 7318 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2497 4078 4225 2257 Size: 240 1581 147 5350 Fragments arranged by size: 5350 1581 240 147 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7318 XmaI C'CCGG_G Cuts at: 1870 2039 2060 2481 4399 4643 1870 Size: 169 21 421 1918 244 4545 Fragments arranged by size: 4545 1918 421 244 169 21 XmnI GAAnn'nnTTC Cuts at: 4268 5447 4268 Size: 1179 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5126 276 Size: 53 83 186 936 3592 2468 Fragments arranged by size: 3592 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI