Restriction map for
pFN21AB8515
(Circular) (Six-base) MAPSORT of: pFN21AB8515.seq Check: 1088 from: 1 to: 7549
pFN21AB8515 7549 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2387 2387
Size: 7549
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3239 4725 5359 278
Size: 53 83 186 936 1703 1486 634 2468
Fragments arranged by size:
2468 1703 1486 936 634 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4233 4892 1937
Size: 2296 659 4594
Fragments arranged by size:
4594 2296 659
Acc65I G'GTAC_C
Cuts at: 4870 4870
Size: 7549
AclI AA'CG_TT
Cuts at: 1563 5676 6049 1563
Size: 4113 373 3063
Fragments arranged by size:
4113 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 2198 2459 2905 3383 3980 4265 4627
Size: 560 6 261 446 478 597 285 362
Cuts at: 4627 5612 6996 7264 1632
Size: 985 1384 268 1917
Fragments arranged by size:
1917 1384 985 597 560 478 446 362
285 268 261 6
AfeI AGC'GCT
Cuts at: 2743 2743
Size: 7549
AflII C'TTAA_G
Cuts at: 820 1017 3206 820
Size: 197 2189 5163
Fragments arranged by size:
5163 2189 197
AflIII A'CryG_T
Cuts at: 1203 4092 4152 4389 6448 7425 1203
Size: 2889 60 237 2059 977 1327
Fragments arranged by size:
2889 2059 1327 977 237 60
AgeI A'CCGG_T
Cuts at: 6380 6380
Size: 7549
AhdI GACnn_n'nnGTC
Cuts at: 6278 6278
Size: 7549
AleI CACnn'nnGTG
Cuts at: 1166 3224 3566 1166
Size: 2058 342 5149
Fragments arranged by size:
5149 2058 342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7549
AlwNI CAG_nnn'CTG
Cuts at: 2038 2125 2293 2302 2812 5292 6864 2038
Size: 87 168 9 510 2480 1572 2723
Fragments arranged by size:
2723 2480 1572 510 168 87 9
ApaI G_GGCC'C
Cuts at: 2237 2943 2237
Size: 706 6843
ApaLI G'TGCA_C
Cuts at: 5606 6762 5606
Size: 1156 6393
ApoI r'AATT_y
Cuts at: 1493 3356 3498 3920 4858 5084 5236 7345 1493
Size: 1863 142 422 938 226 152 2109 1697
Fragments arranged by size:
2109 1863 1697 938 422 226 152 142
AseI AT'TA_AT
Cuts at: 160 1032 6103 160
Size: 872 5071 1606
Fragments arranged by size:
5071 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7549
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 3117 3782 3981 4874 1870
Size: 73 165 1009 665 199 893 4545
Fragments arranged by size:
4545 1009 893 665 199 165 73
BamHI G'GATC_C
Cuts at: 3638 3727 4879 5246 3638
Size: 89 1152 367 5941
Fragments arranged by size:
5941 1152 367 89
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2679 2714 3611 4677 4870
Size: 325 217 627 892 35 897 1066 193
Cuts at: 4870 6325 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1066 897 892 627 325 217
193 35
BanII G_rGCy'C
Cuts at: 1391 2237 2261 2898 2943 3722 3758 4167 4772
Size: 846 24 637 45 779 36 409 605
Cuts at: 4772 4868 1391
Size: 96 4072
Fragments arranged by size:
4072 846 779 637 605 409 96 45
36 24
BbeI G_GCGC'C
Cuts at: 4681 4681
Size: 7549
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4483 928
Size: 971 2584 3994
Fragments arranged by size:
3994 2584 971
BbvCI CC'TCA_GC
Cuts at: 3438 3438
Size: 7549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4595 4629 5740 5774 1903
Size: 34 2658 34 1111 34 3678
Fragments arranged by size:
3678 2658 1111 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5441 6651 5441
Size: 1210 6339
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7549
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3150 3953 4670
Size: 107 122 71 631 605 1478 803 717
Cuts at: 4670 6160 136
Size: 1490 1525
Fragments arranged by size:
1525 1490 1478 803 717 631 605 122
107 71
BglII A'GATC_T
Cuts at: 7544 7544
Size: 7549
BlpI GC'TnA_GC
Cuts at: 2738 4966 2738
Size: 2228 5321
Bme1580I G_kGCm'C
Cuts at: 1790 2237 2943 3301 3352 3608 4701 5610 6766
Size: 447 706 358 51 256 1093 909 1156
Cuts at: 6766 1790
Size: 2573
Fragments arranged by size:
2573 1156 1093 909 706 447 358 256
51
BmgBI CAC'GTC
Cuts at: 1318 4153 4392 1318
Size: 2835 239 4475
Fragments arranged by size:
4475 2835 239
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2347 3924 4433
Size: 416 53 83 399 24 930 1577 509
Cuts at: 4433 5280 6238 442
Size: 847 958 1753
Fragments arranged by size:
1753 1577 958 930 847 509 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7549
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2067 2912 3297 3492 2067
Size: 845 385 195 6124
Fragments arranged by size:
6124 845 385 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2147 2153 2435 2621 2855 3020 3305
Size: 821 87 6 282 186 234 165 285
Cuts at: 3305 3329 3618 6209 1239
Size: 24 289 2591 2579
Fragments arranged by size:
2591 2579 821 289 285 282 234 186
165 87 24 6
Bpu10I CC'TnA_GC
Cuts at: 2396 3104 3438 3752 2396
Size: 708 334 314 6193
Fragments arranged by size:
6193 708 334 314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5674 6539 6837 5674
Size: 865 298 6386
Fragments arranged by size:
6386 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3018 3029 3791 3800 6212 882
Size: 617 1519 11 762 9 2412 2219
Fragments arranged by size:
2412 2219 1519 762 617 11 9
BsaAI yAC'GTr
Cuts at: 493 1204 2314 3564 4093 5340 493
Size: 711 1110 1250 529 1247 2702
Fragments arranged by size:
2702 1250 1247 1110 711 529
BsaBI GATnn'nnATC
Cuts at: 2555 3548 7543 2555
Size: 993 3995 2561
Fragments arranged by size:
3995 2561 993
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3236 4678 4722 5356
Size: 53 83 186 936 1703 1442 44 634
Cuts at: 5356 5738 6373 275
Size: 382 635 1451
Fragments arranged by size:
1703 1451 1442 936 635 634 382 186
83 53 44
BsaWI w'CCGG_w
Cuts at: 2499 3579 4084 4332 5982 6380 6654 6801 7212
Size: 1080 505 248 1650 398 274 147 411
Cuts at: 7212 2499
Size: 2836
Fragments arranged by size:
2836 1650 1080 505 411 398 274 248
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 4079 4109 1178
Size: 30 165 30 1005 30 1641 30 4618
Fragments arranged by size:
4618 1641 1005 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2251 2899 3902 4496 4499 4754 1186
Size: 1065 648 1003 594 3 255 3981
Fragments arranged by size:
3981 1065 1003 648 594 255 3
BseYI C'CCAG_C
Cuts at: 2077 2520 2812 3112 3883 4198 4594 4656 6752
Size: 443 292 300 771 315 396 62 2096
Cuts at: 6752 7248 2077
Size: 496 2378
Fragments arranged by size:
2378 2096 771 496 443 396 315 300
292 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 2288 3422 3847 4121 1163
Size: 573 531 21 1134 425 274 4591
Fragments arranged by size:
4591 1134 573 531 425 274 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4119 4542 4714 4999 5760 6788
Size: 275 186 2118 423 172 285 761 1028
Cuts at: 6788 1540
Size: 2301
Fragments arranged by size:
2301 2118 1028 761 423 285 275 186
172
BsiHKAI G_wGCw'C
Cuts at: 2700 2898 3722 3758 3912 4092 5610 5695 6766
Size: 198 824 36 154 180 1518 85 1071
Cuts at: 6766 2700
Size: 3483
Fragments arranged by size:
3483 1518 1071 824 198 180 154 85
36
BsmI GAATG_Cn'
Cuts at: 5060 5153 5060
Size: 93 7456
BsmBI CGTCTCn'nnnn_
Cuts at: 3978 3978
Size: 7549
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2261 2700 2898 2943 3301 3352
Size: 399 447 24 439 198 45 358 51
Cuts at: 3352 3608 3722 3758 3912 4092 4167 4701 4772
Size: 256 114 36 154 180 75 534 71
Cuts at: 4772 4868 5610 5695 6766 1391
Size: 96 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 534 447 439 399 358
256 198 180 154 114 96 85 75
71 51 45 36 24
BspEI T'CCGG_A
Cuts at: 3579 7212 3579
Size: 3633 3916
BspHI T'CATG_A
Cuts at: 4557 5438 7168 4557
Size: 881 1730 4938
Fragments arranged by size:
4938 1730 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2205 2286 2387 4904 844
Size: 1361 81 101 2517 3489
Fragments arranged by size:
3489 2517 1361 101 81
BsrBI CCG'CTC
Cuts at: 1394 1823 2137 2143 2839 2986 4588 5436 1394
Size: 429 314 6 696 147 1602 848 3507
Fragments arranged by size:
3507 1602 848 696 429 314 147 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 2488 6044 6218 65
Size: 1183 1240 3556 174 1396
Fragments arranged by size:
3556 1396 1240 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2591 4118 6193 6380 1700
Size: 110 781 1527 2075 187 2869
Fragments arranged by size:
2869 2075 1527 781 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7549
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7549
BssSI C'ACGA_G
Cuts at: 5609 6621 5609
Size: 1012 6537
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2302 2530 2970 3400 794
Size: 1508 228 440 430 4943
Fragments arranged by size:
4943 1508 440 430 228
BstBI TT'CG_AA
Cuts at: 6353 6353
Size: 7549
BstEII G'GTnAC_C
Cuts at: 1184 3442 1184
Size: 2258 5291
BstXI CCAn_nnnn'nTGG
Cuts at: 2218 2360 2218
Size: 142 7407
BstYI r'GATC_y
Cuts at: 1972 3638 3727 4598 4879 5246 5631 5648 7100
Size: 1666 89 871 281 367 385 17 1452
Cuts at: 7100 7186 7198 7544 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1666 1452 871 385 367 346 281
89 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4374 4374
Size: 7549
BtgI C'CryG_G
Cuts at: 513 1065 2004 3044 3600 3654 4800 513
Size: 552 939 1040 556 54 1146 3262
Fragments arranged by size:
3262 1146 1040 939 556 552 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6810
Fragments arranged by size:
6810 683 56
BtsI GCAGTG_nn'
Cuts at: 3774 4035 5065 5859 5879 3774
Size: 261 1030 794 20 5444
Fragments arranged by size:
5444 1030 794 261 20
ClaI AT'CG_AT
Cuts at: 1554 3552 1554
Size: 1998 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7514
DraI TTT'AAA
Cuts at: 4853 4853
Size: 7549
DraIII CAC_nnn'GTG
Cuts at: 2317 2704 2970 2317
Size: 387 266 6896
Fragments arranged by size:
6896 387 266
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 4502 6556 809
Size: 507 3074 112 2054 1802
Fragments arranged by size:
3074 2054 1802 507 112
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2334 2385
Size: 54 1425 50 275 18 255 249 51
Cuts at: 2385 2427 2593 2706 2934 3151 4116 4539 4711
Size: 42 166 113 228 217 965 423 172
Cuts at: 4711 4836 4996 5885 7514 8
Size: 125 160 889 1629 43
Fragments arranged by size:
1629 1425 965 889 423 275 255 249
228 217 172 166 160 125 113 54
51 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 4116 4539 4711 4996 1537
Size: 275 2304 423 172 285 4090
Fragments arranged by size:
4090 2304 423 285 275 172
EarI CTCTTCn'nnn_
Cuts at: 1353 2664 2886 3710 4070 5479 1353
Size: 1311 222 824 360 1409 3423
Fragments arranged by size:
3423 1409 1311 824 360 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2471 2564 4174 6136 6510
Size: 1064 138 435 447 93 1610 1962 374
Cuts at: 6510 6656 387
Size: 146 1280
Fragments arranged by size:
1962 1610 1280 1064 447 435 374 146
138 93
EcoICRI GAG'CTC
Cuts at: 2896 3720 3756 2896
Size: 824 36 6689
Fragments arranged by size:
6689 824 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2147 2153 2192 2198 2435 2459
Size: 393 428 87 6 39 6 237 24
Cuts at: 2459 2621 2855 2905 3020 3305 3329 3383 3618
Size: 162 234 50 115 285 24 54 235
Cuts at: 3618 3980 4265 4627 5612 6209 6996 7264 1239
Size: 362 285 362 985 597 787 268 1524
Fragments arranged by size:
1524 985 787 597 428 393 362 362
285 285 268 237 235 234 162 115
87 54 50 39 24 24 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4307 4642 4307
Size: 335 7214
EcoO109I rG'GnC_Cy
Cuts at: 2234 2752 2940 2994 3071 3078 3121 3340 3815
Size: 518 188 54 77 7 43 219 475
Cuts at: 3815 4126 4240 4353 4756 2234
Size: 311 114 113 403 5027
Fragments arranged by size:
5027 518 475 403 311 219 188 114
113 77 54 43 7
EcoRI G'AATT_C
Cuts at: 3920 4858 5236 3920
Size: 938 378 6233
Fragments arranged by size:
6233 938 378
FseI GG_CCGG'CC
Cuts at: 2595 2595
Size: 7549
FspI TGC'GCA
Cuts at: 2663 6055 2663
Size: 3392 4157
HaeII r_GCGC'y
Cuts at: 1678 2585 2745 4681 6696 1678
Size: 907 160 1936 2015 2531
Fragments arranged by size:
2531 2015 1936 907 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4142 4174 6190 6222 6264
Size: 32 135 32 2570 32 2016 32 42
Cuts at: 6264 6296 1373
Size: 32 2626
Fragments arranged by size:
2626 2570 2016 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4507 4893 5139 669
Size: 988 281 2569 386 246 3079
Fragments arranged by size:
3079 2569 988 386 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7549
HpaI GTT'AAC
Cuts at: 5139 5139
Size: 7549
KasI G'GCGC_C
Cuts at: 4677 4677
Size: 7549
KpnI G_GTAC'C
Cuts at: 4874 4874
Size: 7549
MfeI C'AATT_G
Cuts at: 5148 5148
Size: 7549
MluI A'CGCG_T
Cuts at: 7425 7425
Size: 7549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2826 3348 3508 4230 6663 6847 1305
Size: 1521 522 160 722 2433 184 2007
Fragments arranged by size:
2433 2007 1521 722 522 184 160
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2429 10
Size: 54 1425 343 597 5130
Fragments arranged by size:
5130 1425 597 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 3224 3566 3668 5507 5866 6025
Size: 648 1398 660 342 102 1839 359 159
Cuts at: 6025 518
Size: 2042
Fragments arranged by size:
2042 1839 1398 660 648 359 342 159
102
MspA1I CmG'CkG
Cuts at: 1606 2058 2077 2125 2293 2599 2915 3337 3406
Size: 452 19 48 168 306 316 422 69
Cuts at: 3406 4802 4965 5642 6790 7035 1606
Size: 1396 163 677 1148 245 2120
Fragments arranged by size:
2120 1396 1148 677 452 422 316 306
245 168 163 69 48 19
NaeI GCC'GGC
Cuts at: 2593 2593
Size: 7549
NarI GG'CG_CC
Cuts at: 4678 4678
Size: 7549
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6058
Fragments arranged by size:
6058 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7549
NgoMIV G'CCGG_C
Cuts at: 2591 2591
Size: 7549
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7549
NotI GC'GGCC_GC
Cuts at: 4996 4996
Size: 7549
NspI r_CATG'y
Cuts at: 1132 1229 2569 4907 6452 1132
Size: 97 1340 2338 1545 2229
Fragments arranged by size:
2338 2229 1545 1340 97
PasI CC'CwG_GG
Cuts at: 1348 3421 3623 4469 1348
Size: 2073 202 846 4428
Fragments arranged by size:
4428 2073 846 202
PciI A'CATG_T
Cuts at: 6448 6448
Size: 7549
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2812 3045 3264 1108
Size: 1704 233 219 5393
Fragments arranged by size:
5393 1704 233 219
PfoI T'CCnGG_A
Cuts at: 3634 3765 4263 4818 3634
Size: 131 498 555 6365
Fragments arranged by size:
6365 555 498 131
PmeI GTTT'AAAC
Cuts at: 4853 4853
Size: 7549
PmlI CAC'GTG
Cuts at: 2314 3564 4093 2314
Size: 1250 529 5770
Fragments arranged by size:
5770 1250 529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5618 5650 7131 7163 5618
Size: 32 1481 32 6004
Fragments arranged by size:
6004 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2994 3071 3078 4126 4240 4353 2994
Size: 77 7 1048 114 113 6190
Fragments arranged by size:
6190 1048 114 113 77 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7549
PsiI TTA'TAA
Cuts at: 5119 5119
Size: 7549
PspOMI G'GGCC_C
Cuts at: 2233 2939 2233
Size: 706 6843
PstI C_TGCA'G
Cuts at: 830 2298 2589 3023 4901 830
Size: 1468 291 434 1878 3478
Fragments arranged by size:
3478 1878 1468 434 291
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7549
PvuII CAG'CTG
Cuts at: 2058 2077 2125 2293 2599 3406 2058
Size: 19 48 168 306 807 6201
Fragments arranged by size:
6201 807 306 168 48 19
RsrII CG'GwC_CG
Cuts at: 2758 2758
Size: 7549
SacI G_AGCT'C
Cuts at: 2898 3722 3758 2898
Size: 824 36 6689
Fragments arranged by size:
6689 824 36
SacII CC_GC'GG
Cuts at: 4803 4803
Size: 7549
SalI G'TCGA_C
Cuts at: 1936 4891 1936
Size: 2955 4594
SanDI GG'GwC_CC
Cuts at: 2994 3078 4353 2994
Size: 84 1275 6190
Fragments arranged by size:
6190 1275 84
SapI GCTCTTCn'nnn_
Cuts at: 2664 2886 3710 2664
Size: 222 824 6503
Fragments arranged by size:
6503 824 222
SbfI CC_TGCA'GG
Cuts at: 4901 4901
Size: 7549
ScaI AGT'ACT
Cuts at: 4112 5797 7509 4112
Size: 1685 1712 4152
Fragments arranged by size:
4152 1712 1685
SfcI C'TryA_G
Cuts at: 826 1045 2294 2476 2585 2959 3019 4205 4234
Size: 219 1249 182 109 374 60 1186 29
Cuts at: 4234 4897 6032 6713 6904 826
Size: 663 1135 681 191 1471
Fragments arranged by size:
1471 1249 1186 1135 681 663 374 219
191 182 109 60 29
SfoI GGC'GCC
Cuts at: 4679 4679
Size: 7549
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7549
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7549
SmaI CCC'GGG
Cuts at: 1872 2110 3119 4876 1872
Size: 238 1009 1757 4545
Fragments arranged by size:
4545 1757 1009 238
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3206 5653 6554 6816 820
Size: 197 926 1263 2447 901 262 1553
Fragments arranged by size:
2447 1553 1263 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5340 493
Size: 4847 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7549
SphI G_CATG'C
Cuts at: 1132 2569 4907 1132
Size: 1437 2338 3774
Fragments arranged by size:
3774 2338 1437
SspI AAT'ATT
Cuts at: 5 52 5473 5
Size: 47 5421 2081
Fragments arranged by size:
5421 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7549
StyI C'CwwG_G
Cuts at: 513 1065 2004 2493 2629 2748 2871 4988 513
Size: 552 939 489 136 119 123 2117 3074
Fragments arranged by size:
3074 2117 939 552 489 136 123 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 2642 3556 4415 5576 5593 5746
Size: 1255 774 288 914 859 1161 17 153
Cuts at: 5746 5931 6376 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1161 914 859 774 445 288
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4110 5795 7507
Size: 274 80 33 51 1443 2133 1685 1712
Cuts at: 7507 96
Size: 138
Fragments arranged by size:
2133 1712 1685 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3235 3267 3643 3675 4079 4111 4413 4445 7509
Size: 32 376 32 404 32 302 32 3064
Cuts at: 7509 7541 3235
Size: 32 3243
Fragments arranged by size:
3243 3064 404 376 302 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7549
XbaI T'CTAG_A
Cuts at: 4885 4885
Size: 7549
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2215 2809 2215
Size: 594 6955
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7549
XmaI C'CCGG_G
Cuts at: 1870 2108 3117 4874 1870
Size: 238 1009 1757 4545
Fragments arranged by size:
4545 1757 1009 238
XmnI GAAnn'nnTTC
Cuts at: 3500 3524 4064 5678 3500
Size: 24 540 1614 5371
Fragments arranged by size:
5371 1614 540 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3237 4723 5357 276
Size: 53 83 186 936 1703 1486 634 2468
Fragments arranged by size:
2468 1703 1486 936 634 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FspAI NruI NsiI PacI PspXI PsrI SexAI
SfiI SrfI SwaI