Restriction map for
pFN21AB8511
(Circular) (Six-base) MAPSORT of: pFN21AB8511.seq Check: 7459 from: 1 to: 7528
pFN21AB8511 7528 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3575 3697 3575
Size: 122 7406
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5338 278
Size: 53 83 186 936 3802 2468
Fragments arranged by size:
3802 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4871 1937
Size: 2934 4594
Acc65I G'GTAC_C
Cuts at: 4849 4849
Size: 7528
AclI AA'CG_TT
Cuts at: 1563 5655 6028 1563
Size: 4092 373 3063
Fragments arranged by size:
4092 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3273 4337 4400 4526 5591 6975 7243 1632
Size: 1641 1064 63 126 1065 1384 268 1917
Fragments arranged by size:
1917 1641 1384 1065 1064 268 126 63
AfeI AGC'GCT
Cuts at: 4703 4703
Size: 7528
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7331
AflIII A'CryG_T
Cuts at: 1203 6427 7404 1203
Size: 5224 977 1327
Fragments arranged by size:
5224 1327 977
AgeI A'CCGG_T
Cuts at: 6359 6359
Size: 7528
AhdI GACnn_n'nnGTC
Cuts at: 2801 6257 2801
Size: 3456 4072
AleI CACnn'nnGTG
Cuts at: 1166 3302 4237 1166
Size: 2136 935 4457
Fragments arranged by size:
4457 2136 935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7528
AlwNI CAG_nnn'CTG
Cuts at: 5271 6843 5271
Size: 1572 5956
ApaI G_GGCC'C
Cuts at: 2112 3988 2112
Size: 1876 5652
ApaLI G'TGCA_C
Cuts at: 3678 5585 6741 3678
Size: 1907 1156 4465
Fragments arranged by size:
4465 1907 1156
ApoI r'AATT_y
Cuts at: 1493 2836 4837 5063 5215 7324 1493
Size: 1343 2001 226 152 2109 1697
Fragments arranged by size:
2109 2001 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6082 160
Size: 872 5050 1606
Fragments arranged by size:
5050 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7528
AvaI C'yCGr_G
Cuts at: 1870 1943 2486 3194 3771 4029 4284 4463 4853
Size: 73 543 708 577 258 255 179 390
Cuts at: 4853 1870
Size: 4545
Fragments arranged by size:
4545 708 577 543 390 258 255 179
73
AvrII C'CTAG_G
Cuts at: 2029 2029
Size: 7528
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4420 4453 4420
Size: 33 7495
BamHI G'GATC_C
Cuts at: 2605 3522 4858 5225 2605
Size: 917 1336 367 4908
Fragments arranged by size:
4908 1336 917 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 3845 4849 6304 618
Size: 325 217 627 288 1770 1004 1455 1842
Fragments arranged by size:
1842 1770 1455 1004 627 325 288 217
BanII G_rGCy'C
Cuts at: 1391 2112 3312 3988 4847 1391
Size: 721 1200 676 859 4072
Fragments arranged by size:
4072 1200 859 721 676
BbeI G_GCGC'C
Cuts at: 2079 3849 2079
Size: 1770 5758
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4265 4299 5719 5753 1903
Size: 34 2328 34 1420 34 3678
Fragments arranged by size:
3678 2328 1420 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5420 6630 4448
Size: 972 1210 5346
Fragments arranged by size:
5346 1210 972
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7528
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3911 6139 136
Size: 107 122 71 631 605 2239 2228 1525
Fragments arranged by size:
2239 2228 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3933 4090 7523 3933
Size: 157 3433 3938
Fragments arranged by size:
3938 3433 157
BlpI GC'TnA_GC
Cuts at: 2477 2768 3272 3417 4945 2477
Size: 291 504 145 1528 5060
Fragments arranged by size:
5060 1528 504 291 145
Bme1580I G_kGCm'C
Cuts at: 1790 2112 3023 3682 3988 4021 4427 5589 6745
Size: 322 911 659 306 33 406 1162 1156
Cuts at: 6745 1790
Size: 2573
Fragments arranged by size:
2573 1162 1156 911 659 406 322 306
33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7528
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2979 5259 6217
Size: 416 53 83 399 24 1562 2280 958
Cuts at: 6217 442
Size: 1753
Fragments arranged by size:
2280 1753 1562 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7528
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2766 2766
Size: 7528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3353 3443 3692 3977 4076 4280 4382 6188
Size: 2114 90 249 285 99 204 102 1806
Cuts at: 6188 1239
Size: 2579
Fragments arranged by size:
2579 2114 1806 285 249 204 102 99
90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2257 2423 2882 5653 6518 6816 2257
Size: 166 459 2771 865 298 2969
Fragments arranged by size:
2969 2771 865 459 298 166
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3329 6191 882
Size: 617 1830 2862 2219
Fragments arranged by size:
2862 2219 1830 617
BsaAI yAC'GTr
Cuts at: 493 1204 2056 5319 493
Size: 711 852 3263 2702
Fragments arranged by size:
3263 2702 852 711
BsaBI GATnn'nnATC
Cuts at: 3131 7522 3131
Size: 4391 3137
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2684 3846 5335
Size: 53 83 186 936 543 608 1162 1489
Cuts at: 5335 5717 6352 275
Size: 382 635 1451
Fragments arranged by size:
1489 1451 1162 936 635 608 543 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2582 4433 5961 6359 6633 6780 7191 2582
Size: 1851 1528 398 274 147 411 2919
Fragments arranged by size:
2919 1851 1528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4724 4754 1178
Size: 30 165 30 3321 30 3952
Fragments arranged by size:
3952 3321 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2874 2885 3623 3944 4115 4175 4277 4495
Size: 1688 11 738 321 171 60 102 218
Cuts at: 4495 4691 4703 1186
Size: 196 12 4011
Fragments arranged by size:
4011 1688 738 321 218 196 171 102
60 12 11
BseYI C'CCAG_C
Cuts at: 2065 2258 3551 3716 6731 7227 2065
Size: 193 1293 165 3015 496 2366
Fragments arranged by size:
3015 2366 1293 496 193 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4734 1163
Size: 573 2998 3957
Fragments arranged by size:
3957 2998 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3469 4810 4978 5739 6767 1540
Size: 275 186 1468 1341 168 761 1028 2301
Fragments arranged by size:
2301 1468 1341 1028 761 275 186 168
BsiHKAI G_wGCw'C
Cuts at: 3312 3682 4291 4536 5589 5674 6745 3312
Size: 370 609 245 1053 85 1071 4095
Fragments arranged by size:
4095 1071 1053 609 370 245 85
BsmI GAATG_Cn'
Cuts at: 5039 5132 5039
Size: 93 7435
BsmBI CGTCTCn'nnnn_
Cuts at: 2906 2906
Size: 7528
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 3023 3312 3682 3988 4021 4291
Size: 399 322 911 289 370 306 33 270
Cuts at: 4291 4427 4536 4847 5589 5674 6745 1391
Size: 136 109 311 742 85 1071 2174
Fragments arranged by size:
2174 1071 911 742 399 370 322 311
306 289 270 136 109 85 33
BspEI T'CCGG_A
Cuts at: 7191 7191
Size: 7528
BspHI T'CATG_A
Cuts at: 3000 4038 5417 7147 3000
Size: 1038 1379 1730 3381
Fragments arranged by size:
3381 1730 1379 1038
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3575 3697 4883 844
Size: 2731 122 1186 3489
Fragments arranged by size:
3489 2731 1186 122
BsrBI CCG'CTC
Cuts at: 1394 1823 3982 5415 1394
Size: 429 2159 1433 3507
Fragments arranged by size:
3507 2159 1433 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6023 6197 65
Size: 1183 4775 174 1396
Fragments arranged by size:
4775 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4805 6172 6359 1700
Size: 110 2995 1367 187 2869
Fragments arranged by size:
2995 2869 1367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7528
BssHII G'CGCG_C
Cuts at: 1925 3739 1925
Size: 1814 5714
BssSI C'ACGA_G
Cuts at: 3681 5588 6600 3681
Size: 1907 1012 4609
Fragments arranged by size:
4609 1907 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7528
BstBI TT'CG_AA
Cuts at: 6332 6332
Size: 7528
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7528
BstXI CCAn_nnnn'nTGG
Cuts at: 3085 3304 3499 3862 4495 3085
Size: 219 195 363 633 6118
Fragments arranged by size:
6118 633 363 219 195
BstYI r'GATC_y
Cuts at: 1972 2218 2605 3522 3933 4090 4858 5225 5610
Size: 246 387 917 411 157 768 367 385
Cuts at: 5610 5627 7079 7165 7177 7523 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 917 768 411 387 385 367
346 246 157 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4793 4793
Size: 7528
BtgI C'CryG_G
Cuts at: 513 1065 3084 3763 4542 513
Size: 552 2019 679 779 3499
Fragments arranged by size:
3499 2019 779 679 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2511 4372 4744 487
Size: 683 56 1285 1861 372 3271
Fragments arranged by size:
3271 1861 1285 683 372 56
BtsI GCAGTG_nn'
Cuts at: 3524 3811 4021 5044 5838 5858 3524
Size: 287 210 1023 794 20 5194
Fragments arranged by size:
5194 1023 794 287 210 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7528
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7493
DraI TTT'AAA
Cuts at: 2835 4832 2835
Size: 1997 5531
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6535 809
Size: 507 5219 1802
Fragments arranged by size:
5219 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2734 3561 3999
Size: 54 1425 50 275 18 904 827 438
Cuts at: 3999 4404 4539 4545 4803 4807 4975 5864 7493
Size: 405 135 6 258 4 168 889 1629
Cuts at: 7493 8
Size: 43
Fragments arranged by size:
1629 1425 904 889 827 438 405 275
258 168 135 54 50 43 18 6
4
EagI C'GGCC_G
Cuts at: 1537 1812 4807 4975 1537
Size: 275 2995 168 4090
Fragments arranged by size:
4090 2995 275 168
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 2463 3719 5458 1353
Size: 920 190 1256 1739 3423
Fragments arranged by size:
3423 1739 1256 920 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6115 6489 6635 387
Size: 1064 138 4526 374 146 1280
Fragments arranged by size:
4526 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3310 3310
Size: 7528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3273 3353 3443 3692 3977 4076 4280
Size: 393 1641 80 90 249 285 99 204
Cuts at: 4280 4337 4382 4400 4526 5591 6188 6975 7243
Size: 57 45 18 126 1065 597 787 268
Cuts at: 7243 1239
Size: 1524
Fragments arranged by size:
1641 1524 1065 787 597 393 285 268
249 204 126 99 90 80 57 45
18
EcoNI CCTnn'n_nnAGG
Cuts at: 2706 2706
Size: 7528
EcoO109I rG'GnC_Cy
Cuts at: 4105 4473 4105
Size: 368 7160
EcoRI G'AATT_C
Cuts at: 4837 5215 4837
Size: 378 7150
EcoRV GAT'ATC
Cuts at: 2875 2875
Size: 7528
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4840 4840
Size: 7528
FseI GG_CCGG'CC
Cuts at: 4809 4809
Size: 7528
FspI TGC'GCA
Cuts at: 6034 6034
Size: 7528
HaeII r_GCGC'y
Cuts at: 1678 2079 3849 4415 4705 6675 1678
Size: 401 1770 566 290 1970 2531
Fragments arranged by size:
2531 1970 1770 566 401 290
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2787 2819 2850 2880 2882
Size: 32 135 32 1215 32 31 30 2
Cuts at: 2882 2905 2912 2937 6169 6201 6243 6275 1373
Size: 23 7 25 3232 32 42 32 2626
Fragments arranged by size:
3232 2626 1215 135 42 32 32 32
32 32 31 30 25 23 7 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2809 4872 5118 669
Size: 988 281 871 2063 246 3079
Fragments arranged by size:
3079 2063 988 871 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7528
HpaI GTT'AAC
Cuts at: 5118 5118
Size: 7528
KasI G'GCGC_C
Cuts at: 2075 3845 2075
Size: 1770 5758
KpnI G_GTAC'C
Cuts at: 4853 4853
Size: 7528
MfeI C'AATT_G
Cuts at: 5127 5127
Size: 7528
MluI A'CGCG_T
Cuts at: 7404 7404
Size: 7528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2443 4326 6642 6826 1305
Size: 1138 1883 2316 184 2007
Fragments arranged by size:
2316 2007 1883 1138 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2736 3563 4001 4406 4547
Size: 54 1425 343 904 827 438 405 141
Cuts at: 4547 10
Size: 2991
Fragments arranged by size:
2991 1425 904 827 438 405 343 141
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2753 3302 3446 4237 5486 5845 6004
Size: 648 1587 549 144 791 1249 359 159
Cuts at: 6004 518
Size: 2042
Fragments arranged by size:
2042 1587 1249 791 648 549 359 159
144
MspA1I CmG'CkG
Cuts at: 1606 2518 4567 4944 5621 6769 7014 1606
Size: 912 2049 377 677 1148 245 2120
Fragments arranged by size:
2120 2049 1148 912 677 377 245
NaeI GCC'GGC
Cuts at: 4807 4807
Size: 7528
NarI GG'CG_CC
Cuts at: 2076 3846 2076
Size: 1770 5758
NcoI C'CATG_G
Cuts at: 513 1065 3763 513
Size: 552 2698 4278
Fragments arranged by size:
4278 2698 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7528
NgoMIV G'CCGG_C
Cuts at: 4805 4805
Size: 7528
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7528
NotI GC'GGCC_GC
Cuts at: 4975 4975
Size: 7528
NspI r_CATG'y
Cuts at: 1132 1229 3203 4886 6431 1132
Size: 97 1974 1683 1545 2229
Fragments arranged by size:
2229 1974 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4334 1348
Size: 2986 4542
PciI A'CATG_T
Cuts at: 6427 6427
Size: 7528
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4137 4590 1108
Size: 3029 453 4046
Fragments arranged by size:
4046 3029 453
PfoI T'CCnGG_A
Cuts at: 3525 3525
Size: 7528
PmeI GTTT'AAAC
Cuts at: 4832 4832
Size: 7528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2582 2614 5597 5629 7110 7142 2582
Size: 32 2983 32 1481 32 2968
Fragments arranged by size:
2983 2968 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7528
PsiI TTA'TAA
Cuts at: 5098 5098
Size: 7528
PspOMI G'GGCC_C
Cuts at: 2108 3984 2108
Size: 1876 5652
PspXI vC'TCGA_Gb
Cuts at: 3194 4284 4463 3194
Size: 1090 179 6259
Fragments arranged by size:
6259 1090 179
PstI C_TGCA'G
Cuts at: 830 3708 3798 3883 4419 4665 4880 830
Size: 2878 90 85 536 246 215 3478
Fragments arranged by size:
3478 2878 536 246 215 90 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7528
SacI G_AGCT'C
Cuts at: 3312 3312
Size: 7528
SalI G'TCGA_C
Cuts at: 1936 4870 1936
Size: 2934 4594
SbfI CC_TGCA'GG
Cuts at: 3708 4880 3708
Size: 1172 6356
ScaI AGT'ACT
Cuts at: 5776 7488 5776
Size: 1712 5816
SfcI C'TryA_G
Cuts at: 826 1045 3387 3704 3794 3879 4415 4661 4876
Size: 219 2342 317 90 85 536 246 215
Cuts at: 4876 6011 6692 6883 826
Size: 1135 681 191 1471
Fragments arranged by size:
2342 1471 1135 681 536 317 246 219
215 191 90 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 3911 3911
Size: 7528
SfoI GGC'GCC
Cuts at: 2077 3847 2077
Size: 1770 5758
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7528
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7528
SmaI CCC'GGG
Cuts at: 1872 4031 4855 1872
Size: 2159 824 4545
Fragments arranged by size:
4545 2159 824
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2272 2438 2897 3194 4284 4463
Size: 197 926 329 166 459 297 1090 179
Cuts at: 4463 5632 6533 6795 820
Size: 1169 901 262 1553
Fragments arranged by size:
1553 1169 1090 926 901 459 329 297
262 197 179 166
SnaBI TAC'GTA
Cuts at: 493 5319 493
Size: 4826 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7528
SphI G_CATG'C
Cuts at: 1132 4886 1132
Size: 3754 3774
SspI AAT'ATT
Cuts at: 5 52 5452 5
Size: 47 5400 2081
Fragments arranged by size:
5400 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7528
StyI C'CwwG_G
Cuts at: 513 1065 2029 3078 3763 4002 4407 4578 4752
Size: 552 964 1049 685 239 405 171 174
Cuts at: 4752 4967 513
Size: 215 3074
Fragments arranged by size:
3074 1049 964 685 552 405 239 215
174 171
SwaI ATTT'AAAT
Cuts at: 2835 2835
Size: 7528
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3408 3483 4051 5555 5572 5725 5910
Size: 1255 1828 75 568 1504 17 153 185
Cuts at: 5910 6355 325
Size: 445 1498
Fragments arranged by size:
1828 1504 1498 1255 568 445 185 153
75 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5774 7486 96
Size: 274 80 33 51 1443 3797 1712 138
Fragments arranged by size:
3797 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2629 2661 4690 4722 4724 4756 7488 7520 2629
Size: 32 2029 32 2 32 2732 32 2637
Fragments arranged by size:
2732 2637 2029 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7528
XbaI T'CTAG_A
Cuts at: 4864 4864
Size: 7528
XhoI C'TCGA_G
Cuts at: 1943 3194 4284 4463 1943
Size: 1251 1090 179 5008
Fragments arranged by size:
5008 1251 1090 179
XmaI C'CCGG_G
Cuts at: 1870 4029 4853 1870
Size: 2159 824 4545
Fragments arranged by size:
4545 2159 824
XmnI GAAnn'nnTTC
Cuts at: 2024 4823 5657 2024
Size: 2799 834 3895
Fragments arranged by size:
3895 2799 834
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5336 276
Size: 53 83 186 936 3802 2468
Fragments arranged by size:
3802 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI SacI SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII
FspAI NruI NsiI PacI PmlI PpuMI PsrI PvuII
RsrII SacII SanDI SapI SexAI SrfI XcmI