Restriction map for
pFN21AB8506
(Circular) (Six-base) MAPSORT of: pFN21AB8506.seq Check: 1848 from: 1 to: 7303
pFN21AB8506 7303 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5113 278
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2856 4646 1937
Size: 919 1790 4594
Fragments arranged by size:
4594 1790 919
Acc65I G'GTAC_C
Cuts at: 4624 4624
Size: 7303
AclI AA'CG_TT
Cuts at: 1563 4602 5430 5803 1563
Size: 3039 828 373 3063
Fragments arranged by size:
3063 3039 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 2675 2685 3041 3126 3219 5366 6750
Size: 968 75 10 356 85 93 2147 1384
Cuts at: 6750 7018 1632
Size: 268 1917
Fragments arranged by size:
2147 1917 1384 968 356 268 93 85
75 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7106
AflIII A'CryG_T
Cuts at: 1203 2125 2135 6202 7179 1203
Size: 922 10 4067 977 1327
Fragments arranged by size:
4067 1327 977 922 10
AgeI A'CCGG_T
Cuts at: 6134 6134
Size: 7303
AhdI GACnn_n'nnGTC
Cuts at: 2863 6032 2863
Size: 3169 4134
AleI CACnn'nnGTG
Cuts at: 1166 3227 3711 1166
Size: 2061 484 4758
Fragments arranged by size:
4758 2061 484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2043 1184
Size: 859 6444
AlwNI CAG_nnn'CTG
Cuts at: 2195 2416 3466 4483 5046 6618 2195
Size: 221 1050 1017 563 1572 2880
Fragments arranged by size:
2880 1572 1050 1017 563 221
ApaI G_GGCC'C
Cuts at: 4538 4538
Size: 7303
ApaLI G'TGCA_C
Cuts at: 5360 6516 5360
Size: 1156 6147
ApoI r'AATT_y
Cuts at: 1493 3152 3797 4612 4838 4990 7099 1493
Size: 1659 645 815 226 152 2109 1697
Fragments arranged by size:
2109 1697 1659 815 645 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3492 5857 160
Size: 872 2460 2365 1606
Fragments arranged by size:
2460 2365 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 3430 4628 1870
Size: 73 1230 257 1198 4545
Fragments arranged by size:
4545 1230 1198 257 73
BamHI G'GATC_C
Cuts at: 2498 2641 4633 5000 2498
Size: 143 1992 367 4801
Fragments arranged by size:
4801 1992 367 143
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4624 6079 618
Size: 325 217 627 2837 1455 1842
Fragments arranged by size:
2837 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3681 4082 4538 4622 1391
Size: 2290 401 456 84 4072
Fragments arranged by size:
4072 2290 456 401 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2354 928
Size: 971 455 5877
Fragments arranged by size:
5877 971 455
BbvCI CC'TCA_GC
Cuts at: 3353 3353
Size: 7303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5494 5528 1903
Size: 34 3557 34 3678
Fragments arranged by size:
3678 3557 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5195 6405 5195
Size: 1210 6093
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7303
BfrBI ATG'CAT
Cuts at: 4518 4518
Size: 7303
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5914 136
Size: 107 122 71 631 605 4242 1525
Fragments arranged by size:
4242 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2482 7298 2482
Size: 4816 2487
BlpI GC'TnA_GC
Cuts at: 3573 4720 3573
Size: 1147 6156
Bme1580I G_kGCm'C
Cuts at: 1790 3985 4538 5364 6520 1790
Size: 2195 553 826 1156 2573
Fragments arranged by size:
2573 2195 1156 826 553
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2215 2401 3085
Size: 416 53 83 399 24 798 186 684
Cuts at: 3085 5034 5992 442
Size: 1949 958 1753
Fragments arranged by size:
1949 1753 958 798 684 416 399 186
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3487 3644 3487
Size: 157 7146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2478 3033 3236 3278 3665 4242 5963
Size: 953 286 555 203 42 387 577 1721
Cuts at: 5963 1239
Size: 2579
Fragments arranged by size:
2579 1721 953 577 555 387 286 203
42
Bpu10I CC'TnA_GC
Cuts at: 3353 3353
Size: 7303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5428 6293 6591 5428
Size: 865 298 6140
Fragments arranged by size:
6140 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2192 2871 5966 882
Size: 617 693 679 3095 2219
Fragments arranged by size:
3095 2219 693 679 617
BsaAI yAC'GTr
Cuts at: 493 1204 5094 493
Size: 711 3890 2702
Fragments arranged by size:
3890 2702 711
BsaBI GATnn'nnATC
Cuts at: 7297 7297
Size: 7303
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5110 5492 6127 275
Size: 53 83 186 936 3577 382 635 1451
Fragments arranged by size:
3577 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5736 6134 6408 6555 6966 5736
Size: 398 274 147 411 6073
Fragments arranged by size:
6073 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7078
Fragments arranged by size:
7078 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3351 4018 4039 4207 1186
Size: 2165 667 21 168 4282
Fragments arranged by size:
4282 2165 667 168 21
BseYI C'CCAG_C
Cuts at: 2067 3659 4072 6506 7002 2067
Size: 1592 413 2434 496 2368
Fragments arranged by size:
2434 2368 1592 496 413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3029 1163
Size: 573 1293 5437
Fragments arranged by size:
5437 1293 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4753 5514 6542 1540
Size: 275 186 2752 761 1028 2301
Fragments arranged by size:
2752 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3373 3681 4438 5364 5449 6520 3373
Size: 308 757 926 85 1071 4156
Fragments arranged by size:
4156 1071 926 757 308 85
BsmI GAATG_Cn'
Cuts at: 2817 3833 4520 4814 4907 2817
Size: 1016 687 294 93 5213
Fragments arranged by size:
5213 1016 687 294 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3373 3681 3985 4082 4438 4538 4622
Size: 399 1583 308 304 97 356 100 84
Cuts at: 4622 5364 5449 6520 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1583 1071 742 399 356 308 304
100 97 85 84
BspEI T'CCGG_A
Cuts at: 6966 6966
Size: 7303
BspHI T'CATG_A
Cuts at: 5192 6922 5192
Size: 1730 5573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4658 844
Size: 3814 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5190 1394
Size: 429 3367 3507
Fragments arranged by size:
3507 3367 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 5798 5972 65
Size: 1183 1239 3311 174 1396
Fragments arranged by size:
3311 1396 1239 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 3804 5947 6134 1700
Size: 110 226 1768 2143 187 2869
Fragments arranged by size:
2869 2143 1768 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7303
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7303
BssSI C'ACGA_G
Cuts at: 3372 3443 5363 6375 3372
Size: 71 1920 1012 4300
Fragments arranged by size:
4300 1920 1012 71
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7303
BstBI TT'CG_AA
Cuts at: 6107 6107
Size: 7303
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7303
BstXI CCAn_nnnn'nTGG
Cuts at: 4094 4094
Size: 7303
BstYI r'GATC_y
Cuts at: 1972 2191 2482 2498 2641 2877 4421 4633 5000
Size: 219 291 16 143 236 1544 212 367
Cuts at: 5000 5385 5402 6854 6940 6952 7298 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1544 1452 385 367 346 291 236
219 212 143 86 17 16 12
Bsu36I CC'TnA_GG
Cuts at: 3452 3452
Size: 7303
BtgI C'CryG_G
Cuts at: 513 1065 3306 3851 513
Size: 552 2241 545 3965
Fragments arranged by size:
3965 2241 552 545
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4233 487
Size: 683 56 3007 3557
Fragments arranged by size:
3557 3007 683 56
BtsI GCAGTG_nn'
Cuts at: 3017 3222 3234 3405 4819 5613 5633 3017
Size: 205 12 171 1414 794 20 4687
Fragments arranged by size:
4687 1414 794 205 171 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3079 3114 529
Size: 35 2515 35 4718
Fragments arranged by size:
4718 2515 35 35
DraI TTT'AAA
Cuts at: 2122 4607 2122
Size: 2485 4818
DraIII CAC_nnn'GTG
Cuts at: 3959 3959
Size: 7303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2511 6310 809
Size: 507 1195 3799 1802
Fragments arranged by size:
3799 1802 1195 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2748 3343 4750
Size: 54 1425 50 275 18 918 595 1407
Cuts at: 4750 5639 7268 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1407 918 889 595 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4750 1537
Size: 275 2938 4090
Fragments arranged by size:
4090 2938 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 2760 2766 3848 4225 5233 1353
Size: 693 714 6 1082 377 1008 3423
Fragments arranged by size:
3423 1082 1008 714 693 377 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4030 5890 6264 6410 387
Size: 1064 138 2441 1860 374 146 1280
Fragments arranged by size:
2441 1860 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3679 3679
Size: 7303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2478 2600 2675 2685 3033 3041
Size: 393 560 286 122 75 10 348 8
Cuts at: 3041 3126 3219 3236 3278 3665 4242 5366 5963
Size: 85 93 17 42 387 577 1124 597
Cuts at: 5963 6750 7018 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1124 787 597 577 560 393 387
348 286 268 122 93 85 75 42
17 10 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3620 4460 3620
Size: 840 6463
EcoO109I rG'GnC_Cy
Cuts at: 2455 3448 3593 3625 4480 4535 2455
Size: 993 145 32 855 55 5223
Fragments arranged by size:
5223 993 855 145 55 32
EcoRI G'AATT_C
Cuts at: 3797 4612 4990 3797
Size: 815 378 6110
Fragments arranged by size:
6110 815 378
EcoRV GAT'ATC
Cuts at: 4242 4242
Size: 7303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3538 3538
Size: 7303
FspI TGC'GCA
Cuts at: 5809 5809
Size: 7303
HaeII r_GCGC'y
Cuts at: 1678 6450 1678
Size: 4772 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2176 2208 2369
Size: 32 135 32 452 32 120 32 161
Cuts at: 2369 2401 2849 2881 3680 3712 5944 5976 6018
Size: 32 448 32 799 32 2232 32 42
Cuts at: 6018 6050 1373
Size: 32 2626
Fragments arranged by size:
2626 2232 799 452 448 161 135 120
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4647 4893 669
Size: 988 281 2709 246 3079
Fragments arranged by size:
3079 2709 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2517 748
Size: 1769 5534
HpaI GTT'AAC
Cuts at: 4893 4893
Size: 7303
KpnI G_GTAC'C
Cuts at: 4628 4628
Size: 7303
MfeI C'AATT_G
Cuts at: 4902 4902
Size: 7303
MluI A'CGCG_T
Cuts at: 7179 7179
Size: 7303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6417 6601 1305
Size: 5112 184 2007
Fragments arranged by size:
5112 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2750 10
Size: 54 1425 343 918 4563
Fragments arranged by size:
4563 1425 918 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3227 3711 5261 5620 5779 518
Size: 648 2061 484 1550 359 159 2042
Fragments arranged by size:
2061 2042 1550 648 484 359 159
MspA1I CmG'CkG
Cuts at: 1606 2446 3256 3685 4076 4719 5396 6544 6789
Size: 840 810 429 391 643 677 1148 245
Cuts at: 6789 1606
Size: 2120
Fragments arranged by size:
2120 1148 840 810 677 643 429 391
245
NaeI GCC'GGC
Cuts at: 2038 3806 2038
Size: 1768 5535
NcoI C'CATG_G
Cuts at: 513 1065 3306 513
Size: 552 2241 4510
Fragments arranged by size:
4510 2241 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7303
NgoMIV G'CCGG_C
Cuts at: 2036 3804 2036
Size: 1768 5535
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7303
NotI GC'GGCC_GC
Cuts at: 4750 4750
Size: 7303
NsiI A_TGCA'T
Cuts at: 4520 4520
Size: 7303
NspI r_CATG'y
Cuts at: 1132 1229 2129 2430 4661 6206 1132
Size: 97 900 301 2231 1545 2229
Fragments arranged by size:
2231 2229 1545 900 301 97
PasI CC'CwG_GG
Cuts at: 1348 4087 1348
Size: 2739 4564
PciI A'CATG_T
Cuts at: 2125 6202 2125
Size: 4077 3226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2328 3959 1108
Size: 1220 1631 4452
Fragments arranged by size:
4452 1631 1220
PfoI T'CCnGG_A
Cuts at: 2494 2523 2494
Size: 29 7274
PmeI GTTT'AAAC
Cuts at: 4607 4607
Size: 7303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5372 5404 6885 6917 5372
Size: 32 1481 32 5758
Fragments arranged by size:
5758 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3593 3625 3593
Size: 32 7271
PshAI GACnn'nnGTC
Cuts at: 1613 2943 1613
Size: 1330 5973
PsiI TTA'TAA
Cuts at: 4873 4873
Size: 7303
PspOMI G'GGCC_C
Cuts at: 4534 4534
Size: 7303
PspXI vC'TCGA_Gb
Cuts at: 3173 3430 3173
Size: 257 7046
PstI C_TGCA'G
Cuts at: 830 3229 4655 830
Size: 2399 1426 3478
Fragments arranged by size:
3478 2399 1426
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7303
PvuII CAG'CTG
Cuts at: 3256 3685 3256
Size: 429 6874
SacI G_AGCT'C
Cuts at: 3681 3681
Size: 7303
SalI G'TCGA_C
Cuts at: 1936 4645 1936
Size: 2709 4594
SapI GCTCTTCn'nnn_
Cuts at: 2766 2766
Size: 7303
SbfI CC_TGCA'GG
Cuts at: 4655 4655
Size: 7303
ScaI AGT'ACT
Cuts at: 2918 5551 7263 2918
Size: 2633 1712 2958
Fragments arranged by size:
2958 2633 1712
SexAI A'CCwGG_T
Cuts at: 3955 3955
Size: 7303
SfcI C'TryA_G
Cuts at: 826 1045 2553 2853 2889 3225 4178 4651 5786
Size: 219 1508 300 36 336 953 473 1135
Cuts at: 5786 6467 6658 826
Size: 681 191 1471
Fragments arranged by size:
1508 1471 1135 953 681 473 336 300
219 191 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7303
SmaI CCC'GGG
Cuts at: 1872 4630 1872
Size: 2758 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3173 3430 5407 6308 6570 820
Size: 197 926 1230 257 1977 901 262 1553
Fragments arranged by size:
1977 1553 1230 926 901 262 257 197
SnaBI TAC'GTA
Cuts at: 493 5094 493
Size: 4601 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7303
SphI G_CATG'C
Cuts at: 1132 4661 1132
Size: 3529 3774
SspI AAT'ATT
Cuts at: 5 52 3503 4452 5227 5
Size: 47 3451 949 775 2081
Fragments arranged by size:
3451 2081 949 775 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7303
StyI C'CwwG_G
Cuts at: 513 1065 3306 4742 513
Size: 552 2241 1436 3074
Fragments arranged by size:
3074 2241 1436 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2316 3836 4244 5330 5347 5500 5685
Size: 1255 736 1520 408 1086 17 153 185
Cuts at: 5685 6130 325
Size: 445 1498
Fragments arranged by size:
1520 1498 1255 1086 736 445 408 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2916 3889 4154
Size: 274 80 33 51 1443 939 973 265
Cuts at: 4154 5549 7261 96
Size: 1395 1712 138
Fragments arranged by size:
1712 1443 1395 973 939 274 265 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2089 2121 7263 7295 2089
Size: 32 5142 32 2097
Fragments arranged by size:
5142 2097 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7303
XbaI T'CTAG_A
Cuts at: 2080 4639 2080
Size: 2559 4744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4469 4469
Size: 7303
XhoI C'TCGA_G
Cuts at: 1943 3173 3430 1943
Size: 1230 257 5816
Fragments arranged by size:
5816 1230 257
XmaI C'CCGG_G
Cuts at: 1870 4628 1870
Size: 2758 4545
XmnI GAAnn'nnTTC
Cuts at: 4362 5432 4362
Size: 1070 6233
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5111 276
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I FseI FspAI KasI NarI NruI PacI
PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI