Restriction map for
pFN21AB8505
(Circular) (Six-base) MAPSORT of: pFN21AB8505.seq Check: 3062 from: 1 to: 7456
pFN21AB8505 7456 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5266 278
Size: 53 83 186 936 3730 2468
Fragments arranged by size:
3730 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4799 1937
Size: 2862 4594
Acc65I G'GTAC_C
Cuts at: 4777 4777
Size: 7456
AclI AA'CG_TT
Cuts at: 1563 3025 3405 5583 5956 1563
Size: 1462 380 2178 373 3063
Fragments arranged by size:
3063 2178 1462 380 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 2461 3744 5519 6903 7171 1632
Size: 436 393 1283 1775 1384 268 1917
Fragments arranged by size:
1917 1775 1384 1283 436 393 268
AflII C'TTAA_G
Cuts at: 820 1017 3811 820
Size: 197 2794 4465
Fragments arranged by size:
4465 2794 197
AflIII A'CryG_T
Cuts at: 1203 3980 6355 7332 1203
Size: 2777 2375 977 1327
Fragments arranged by size:
2777 2375 1327 977
AgeI A'CCGG_T
Cuts at: 6287 6287
Size: 7456
AhdI GACnn_n'nnGTC
Cuts at: 2592 6185 2592
Size: 3593 3863
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4403 1184
Size: 3219 4237
AlwNI CAG_nnn'CTG
Cuts at: 3383 5199 6771 3383
Size: 1816 1572 4068
Fragments arranged by size:
4068 1816 1572
ApaI G_GGCC'C
Cuts at: 4718 4718
Size: 7456
ApaLI G'TGCA_C
Cuts at: 4065 5513 6669 4065
Size: 1448 1156 4852
Fragments arranged by size:
4852 1448 1156
ApoI r'AATT_y
Cuts at: 1493 2344 3340 4765 4991 5143 7252 1493
Size: 851 996 1425 226 152 2109 1697
Fragments arranged by size:
2109 1697 1425 996 851 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2411 6010 160
Size: 872 1379 3599 1606
Fragments arranged by size:
3599 1606 1379 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
AvaI C'yCGr_G
Cuts at: 1870 1943 4781 1870
Size: 73 2838 4545
Fragments arranged by size:
4545 2838 73
AvrII C'CTAG_G
Cuts at: 3398 3398
Size: 7456
BamHI G'GATC_C
Cuts at: 4786 5153 4786
Size: 367 7089
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4777 6232 618
Size: 325 217 627 2990 1455 1842
Fragments arranged by size:
2990 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2426 4440 4718 4775 1391
Size: 1035 2014 278 57 4072
Fragments arranged by size:
4072 2014 1035 278 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2588 3157 3651 4479 928
Size: 971 689 569 494 828 3905
Fragments arranged by size:
3905 971 828 689 569 494
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5647 5681 1903
Size: 34 3710 34 3678
Fragments arranged by size:
3710 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5348 6558 5348
Size: 1210 6246
BclI T'GATC_A
Cuts at: 1548 3952 1548
Size: 2404 5052
BfrBI ATG'CAT
Cuts at: 4586 4586
Size: 7456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3368 6067 136
Size: 107 122 71 631 605 1696 2699 1525
Fragments arranged by size:
2699 1696 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7451 7451
Size: 7456
BlpI GC'TnA_GC
Cuts at: 2669 4873 2669
Size: 2204 5252
Bme1580I G_kGCm'C
Cuts at: 1790 2176 4069 4718 5517 6673 1790
Size: 386 1893 649 799 1156 2573
Fragments arranged by size:
2573 1893 1156 799 649 386
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 2721 3568
Size: 416 53 83 399 24 762 542 847
Cuts at: 3568 3931 4712 5187 6145 442
Size: 363 781 475 958 1753
Fragments arranged by size:
1753 958 847 781 762 542 475 416
399 363 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7456
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3469 3469
Size: 7456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 4762 6116 1239
Size: 1172 2351 1354 2579
Fragments arranged by size:
2579 2351 1354 1172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2499 4091 4298 5581 6446 6744 2499
Size: 1592 207 1283 865 298 3211
Fragments arranged by size:
3211 1592 1283 865 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6119 882
Size: 617 4620 2219
Fragments arranged by size:
4620 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5247 493
Size: 711 4043 2702
Fragments arranged by size:
4043 2702 711
BsaBI GATnn'nnATC
Cuts at: 3522 7450 3522
Size: 3928 3528
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4424 5263 5645 6280
Size: 53 83 186 936 2891 839 382 635
Cuts at: 6280 275
Size: 1451
Fragments arranged by size:
2891 1451 936 839 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4553 5889 6287 6561 6708 7119 4553
Size: 1336 398 274 147 411 4890
Fragments arranged by size:
4890 1336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4622 4652 1178
Size: 30 165 30 3219 30 3982
Fragments arranged by size:
3982 3219 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2202 4088 1186
Size: 1016 1886 4554
Fragments arranged by size:
4554 1886 1016
BseYI C'CCAG_C
Cuts at: 6659 7155 6659
Size: 496 6960
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4275 1163
Size: 573 2539 4344
Fragments arranged by size:
4344 2539 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4906 5667 6695 1540
Size: 275 186 2905 761 1028 2301
Fragments arranged by size:
2905 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2426 4069 4440 5517 5602 6673 2426
Size: 1643 371 1077 85 1071 3209
Fragments arranged by size:
3209 1643 1077 1071 371 85
BsmI GAATG_Cn'
Cuts at: 4967 5060 4967
Size: 93 7363
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 2426 4069 4440 4718 4775 5517
Size: 399 386 250 1643 371 278 57 742
Cuts at: 5517 5602 6673 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1643 1071 742 399 386 371 278
250 85 57
BspEI T'CCGG_A
Cuts at: 7119 7119
Size: 7456
BspHI T'CATG_A
Cuts at: 2977 3019 4726 5345 7075 2977
Size: 42 1707 619 1730 3358
Fragments arranged by size:
3358 1730 1707 619 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4811 844
Size: 3967 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5343 1394
Size: 429 3520 3507
Fragments arranged by size:
3520 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2558 2955 3884 4096 4284 5951 6125
Size: 1183 1310 397 929 212 188 1667 174
Cuts at: 6125 65
Size: 1396
Fragments arranged by size:
1667 1396 1310 1183 929 397 212 188
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6100 6287 1700
Size: 110 4290 187 2869
Fragments arranged by size:
4290 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7456
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7456
BssSI C'ACGA_G
Cuts at: 5516 6528 5516
Size: 1012 6444
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7456
BstBI TT'CG_AA
Cuts at: 3850 6260 3850
Size: 2410 5046
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7456
BstYI r'GATC_y
Cuts at: 1972 3292 3889 4077 4786 5153 5538 5555 7007
Size: 1320 597 188 709 367 385 17 1452
Cuts at: 7007 7093 7105 7451 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1320 709 597 385 367 346
188 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2430 2430
Size: 7456
BtgI C'CryG_G
Cuts at: 513 1065 2004 2445 513
Size: 552 939 441 5524
Fragments arranged by size:
5524 939 552 441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6717
Fragments arranged by size:
6717 683 56
BtsI GCAGTG_nn'
Cuts at: 3378 4972 5766 5786 3378
Size: 1594 794 20 5048
Fragments arranged by size:
5048 1594 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7456
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7421
DraI TTT'AAA
Cuts at: 2014 4760 2014
Size: 2746 4710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6463 809
Size: 507 5147 1802
Fragments arranged by size:
5147 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 4903 5792
Size: 54 1425 50 275 18 552 2521 889
Cuts at: 5792 7421 8
Size: 1629 43
Fragments arranged by size:
2521 1629 1425 889 552 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4903 1537
Size: 275 3091 4090
Fragments arranged by size:
4090 3091 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2202 3501 5386 1353
Size: 734 115 1299 1885 3423
Fragments arranged by size:
3423 1885 1299 734 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6043 6417 6563 387
Size: 1064 138 4454 374 146 1280
Fragments arranged by size:
4454 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2424 4438 2424
Size: 2014 5442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2411 2461 3744 4762 5519 6116
Size: 393 436 343 50 1283 1018 757 597
Cuts at: 6116 6903 7171 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1283 1018 787 757 597 436 393
343 268 50
EcoO109I rG'GnC_Cy
Cuts at: 4122 4714 4122
Size: 592 6864
EcoRI G'AATT_C
Cuts at: 4765 5143 4765
Size: 378 7078
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2915 3119 2915
Size: 204 7252
FspI TGC'GCA
Cuts at: 5962 5962
Size: 7456
HaeII r_GCGC'y
Cuts at: 1678 6603 1678
Size: 4925 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2578 2610 2690 2722 3494
Size: 32 135 32 1006 32 80 32 772
Cuts at: 3494 3526 4409 4441 6097 6129 6171 6203 1373
Size: 32 883 32 1656 32 42 32 2626
Fragments arranged by size:
2626 1656 1006 883 772 135 80 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4402 4800 5046 669
Size: 988 281 2464 398 246 3079
Fragments arranged by size:
3079 2464 988 398 281 246
HindIII A'AGCT_T
Cuts at: 748 3615 4026 4207 748
Size: 2867 411 181 3997
Fragments arranged by size:
3997 2867 411 181
HpaI GTT'AAC
Cuts at: 4402 5046 4402
Size: 644 6812
KpnI G_GTAC'C
Cuts at: 4781 4781
Size: 7456
MfeI C'AATT_G
Cuts at: 2070 4263 4359 5055 2070
Size: 2193 96 696 4471
Fragments arranged by size:
4471 2193 696 96
MluI A'CGCG_T
Cuts at: 7332 7332
Size: 7456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 6570 6754 1305
Size: 1008 4257 184 2007
Fragments arranged by size:
4257 2007 1008 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 10
Size: 54 1425 343 552 5082
Fragments arranged by size:
5082 1425 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2539 4731 5414 5773 5932 518
Size: 648 1373 2192 683 359 159 2042
Fragments arranged by size:
2192 2042 1373 683 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3668 4872 5549 6697 6942 1606
Size: 2062 1204 677 1148 245 2120
Fragments arranged by size:
2120 2062 1204 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2445 513
Size: 552 939 441 5524
Fragments arranged by size:
5524 939 552 441
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7456
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7456
NotI GC'GGCC_GC
Cuts at: 4903 4903
Size: 7456
NsiI A_TGCA'T
Cuts at: 4588 4588
Size: 7456
NspI r_CATG'y
Cuts at: 1132 1229 2935 3079 3984 4089 4814 6359 1132
Size: 97 1706 144 905 105 725 1545 2229
Fragments arranged by size:
2229 1706 1545 905 725 144 105 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7456
PciI A'CATG_T
Cuts at: 3980 6355 3980
Size: 2375 5081
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7456
PfoI T'CCnGG_A
Cuts at: 4212 4544 4212
Size: 332 7124
PmeI GTTT'AAAC
Cuts at: 4760 4760
Size: 7456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5525 5557 7038 7070 5525
Size: 32 1481 32 5911
Fragments arranged by size:
5911 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4122 4122
Size: 7456
PshAI GACnn'nnGTC
Cuts at: 1613 2946 1613
Size: 1333 6123
PsiI TTA'TAA
Cuts at: 3927 5026 3927
Size: 1099 6357
PspOMI G'GGCC_C
Cuts at: 4714 4714
Size: 7456
PstI C_TGCA'G
Cuts at: 830 3315 4808 830
Size: 2485 1493 3478
Fragments arranged by size:
3478 2485 1493
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7456
PvuII CAG'CTG
Cuts at: 3668 3668
Size: 7456
SacI G_AGCT'C
Cuts at: 2426 4440 2426
Size: 2014 5442
SalI G'TCGA_C
Cuts at: 1936 4798 1936
Size: 2862 4594
SapI GCTCTTCn'nnn_
Cuts at: 2202 2202
Size: 7456
SbfI CC_TGCA'GG
Cuts at: 4808 4808
Size: 7456
ScaI AGT'ACT
Cuts at: 4560 5704 7416 4560
Size: 1144 1712 4600
Fragments arranged by size:
4600 1712 1144
SfcI C'TryA_G
Cuts at: 826 1045 3311 4804 5939 6620 6811 826
Size: 219 2266 1493 1135 681 191 1471
Fragments arranged by size:
2266 1493 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7456
SmaI CCC'GGG
Cuts at: 1872 4783 1872
Size: 2911 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2514 3811 4070 4313 5560 6461
Size: 197 926 571 1297 259 243 1247 901
Cuts at: 6461 6723 820
Size: 262 1553
Fragments arranged by size:
1553 1297 1247 926 901 571 262 259
243 197
SnaBI TAC'GTA
Cuts at: 493 5247 493
Size: 4754 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7456
SphI G_CATG'C
Cuts at: 1132 4814 1132
Size: 3682 3774
SspI AAT'ATT
Cuts at: 5 52 2783 3046 3626 5380 5
Size: 47 2731 263 580 1754 2081
Fragments arranged by size:
2731 2081 1754 580 263 47
StuI AGG'CCT
Cuts at: 1517 4319 1517
Size: 2802 4654
StyI C'CwwG_G
Cuts at: 513 1065 2004 2445 3108 3398 4895 513
Size: 552 939 441 663 290 1497 3074
Fragments arranged by size:
3074 1497 939 663 552 441 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5483 5500 5653 5838 6283 325
Size: 1255 3903 17 153 185 445 1498
Fragments arranged by size:
3903 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2545 4558 5702
Size: 274 80 33 51 1443 568 2013 1144
Cuts at: 5702 7414 96
Size: 1712 138
Fragments arranged by size:
2013 1712 1443 1144 568 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4208 4240 4622 4654 7416 7448 4208
Size: 32 382 32 2762 32 4216
Fragments arranged by size:
4216 2762 382 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7456
XbaI T'CTAG_A
Cuts at: 3583 4009 4792 3583
Size: 426 783 6247
Fragments arranged by size:
6247 783 426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2758 3370 2758
Size: 612 6844
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7456
XmaI C'CCGG_G
Cuts at: 1870 4781 1870
Size: 2911 4545
XmnI GAAnn'nnTTC
Cuts at: 2367 3099 3407 3657 4027 5585 2367
Size: 732 308 250 370 1558 4238
Fragments arranged by size:
4238 1558 732 370 308 250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5264 276
Size: 53 83 186 936 3730 2468
Fragments arranged by size:
3730 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI