Restriction map for
pFN21AB8501
(Circular) (Six-base) MAPSORT of: pFN21AB8501.seq Check: 6591 from: 1 to: 7513
pFN21AB8501 7513 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5323 278
Size: 53 83 186 936 3787 2468
Fragments arranged by size:
3787 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2372 3049 4490 4856 1937
Size: 435 677 1441 366 4594
Fragments arranged by size:
4594 1441 677 435 366
Acc65I G'GTAC_C
Cuts at: 4834 4834
Size: 7513
AclI AA'CG_TT
Cuts at: 1563 5640 6013 1563
Size: 4077 373 3063
Fragments arranged by size:
4077 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2921 3651 4827 5576 6960 7228 1632
Size: 1289 730 1176 749 1384 268 1917
Fragments arranged by size:
1917 1384 1289 1176 749 730 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7316
AflIII A'CryG_T
Cuts at: 1203 4450 6412 7389 1203
Size: 3247 1962 977 1327
Fragments arranged by size:
3247 1962 1327 977
AgeI A'CCGG_T
Cuts at: 6344 6344
Size: 7513
AhdI GACnn_n'nnGTC
Cuts at: 3853 6242 3853
Size: 2389 5124
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7513
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4104 4136 4104
Size: 32 7481
AlwNI CAG_nnn'CTG
Cuts at: 2180 5256 6828 2180
Size: 3076 1572 2865
Fragments arranged by size:
3076 2865 1572
ApaLI G'TGCA_C
Cuts at: 5570 6726 5570
Size: 1156 6357
ApoI r'AATT_y
Cuts at: 1493 2299 2610 2662 2695 2786 2864 2875 3216
Size: 806 311 52 33 91 78 11 341
Cuts at: 3216 3262 3491 3671 3697 3920 4198 4617 4822
Size: 46 229 180 26 223 278 419 205
Cuts at: 4822 5048 5200 7309 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 806 419 341 311 278 229
226 223 205 180 152 91 78 52
46 33 26 11
AseI AT'TA_AT
Cuts at: 160 1032 2976 3411 4274 6067 160
Size: 872 1944 435 863 1793 1606
Fragments arranged by size:
1944 1793 1606 872 863 435
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7513
AvaI C'yCGr_G
Cuts at: 1870 1943 3606 4838 1870
Size: 73 1663 1232 4545
Fragments arranged by size:
4545 1663 1232 73
BamHI G'GATC_C
Cuts at: 3298 4717 4843 5210 3298
Size: 1419 126 367 5601
Fragments arranged by size:
5601 1419 367 126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4834 6289 618
Size: 325 217 627 3047 1455 1842
Fragments arranged by size:
3047 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4832 1391
Size: 3441 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3372 3569 928
Size: 971 1473 197 4872
Fragments arranged by size:
4872 1473 971 197
BbvCI CC'TCA_GC
Cuts at: 2009 2009
Size: 7513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5704 5738 1903
Size: 34 50 34 3683 34 3678
Fragments arranged by size:
3683 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4425 5405 6615 4425
Size: 980 1210 5323
Fragments arranged by size:
5323 1210 980
BclI T'GATC_A
Cuts at: 1548 2595 2685 3655 4032 1548
Size: 1047 90 970 377 5029
Fragments arranged by size:
5029 1047 970 377 90
BfrBI ATG'CAT
Cuts at: 2778 4435 2778
Size: 1657 5856
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6124 136
Size: 107 122 71 631 605 4452 1525
Fragments arranged by size:
4452 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2019 7508 2019
Size: 5489 2024
BlpI GC'TnA_GC
Cuts at: 4930 4930
Size: 7513
Bme1580I G_kGCm'C
Cuts at: 1790 5574 6730 1790
Size: 3784 1156 2573
Fragments arranged by size:
3784 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2172 1318
Size: 854 6659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2557 5244 6202
Size: 416 53 83 399 24 1140 2687 958
Cuts at: 6202 442
Size: 1753
Fragments arranged by size:
2687 1753 1140 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 3060 6173 1239
Size: 854 967 3113 2579
Fragments arranged by size:
3113 2579 967 854
Bpu10I CC'TnA_GC
Cuts at: 2009 2009
Size: 7513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2229 4450 4736 5638 6503 6801 2229
Size: 2221 286 902 865 298 2941
Fragments arranged by size:
2941 2221 902 865 298 286
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2152 6176 882
Size: 617 653 4024 2219
Fragments arranged by size:
4024 2219 653 617
BsaAI yAC'GTr
Cuts at: 493 1204 5304 493
Size: 711 4100 2702
Fragments arranged by size:
4100 2702 711
BsaBI GATnn'nnATC
Cuts at: 7507 7507
Size: 7513
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5320 5702 6337 275
Size: 53 83 186 936 3787 382 635 1451
Fragments arranged by size:
3787 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2190 5946 6344 6618 6765 7176 2190
Size: 3756 398 274 147 411 2527
Fragments arranged by size:
3756 2527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2798 2828 3019 3049 1178
Size: 30 165 30 1395 30 191 30 5642
Fragments arranged by size:
5642 1395 191 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7513
BseYI C'CCAG_C
Cuts at: 6716 7212 6716
Size: 496 7017
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2313 2439 3877 1163
Size: 573 577 126 1438 4799
Fragments arranged by size:
4799 1438 577 573 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2191 4963 5724 6752 1540
Size: 275 186 190 2772 761 1028 2301
Fragments arranged by size:
2772 2301 1028 761 275 190 186
BsiHKAI G_wGCw'C
Cuts at: 5574 5659 6730 5574
Size: 85 1071 6357
Fragments arranged by size:
6357 1071 85
BsmI GAATG_Cn'
Cuts at: 5024 5117 5024
Size: 93 7420
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4832 5574 5659 6730 1391
Size: 399 3042 742 85 1071 2174
Fragments arranged by size:
3042 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7176 7176
Size: 7513
BspHI T'CATG_A
Cuts at: 5402 7132 5402
Size: 1730 5783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4868 844
Size: 4024 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2076 5400 1394
Size: 429 253 3324 3507
Fragments arranged by size:
3507 3324 429 253
BsrDI GCAATG_nn'
Cuts at: 65 1248 3826 6008 6182 65
Size: 1183 2578 2182 174 1396
Fragments arranged by size:
2578 2182 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6157 6344 1700
Size: 110 4347 187 2869
Fragments arranged by size:
4347 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7513
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7513
BssSI C'ACGA_G
Cuts at: 2331 5573 6585 2331
Size: 3242 1012 3259
Fragments arranged by size:
3259 3242 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2840 794
Size: 2046 5467
BstBI TT'CG_AA
Cuts at: 6317 6317
Size: 7513
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7513
BstYI r'GATC_y
Cuts at: 1972 2019 3298 4717 4843 5210 5595 5612 7064
Size: 47 1279 1419 126 367 385 17 1452
Cuts at: 7064 7150 7162 7508 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1419 1279 385 367 346 126
86 47 17 12
BstZ17I GTA'TAC
Cuts at: 4491 4491
Size: 7513
Bsu36I CC'TnA_GG
Cuts at: 2082 2082
Size: 7513
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6961
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6774
Fragments arranged by size:
6774 683 56
BtsI GCAGTG_nn'
Cuts at: 3477 5029 5823 5843 3477
Size: 1552 794 20 5147
Fragments arranged by size:
5147 1552 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7513
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4669 4704 529
Size: 35 4105 35 3338
Fragments arranged by size:
4105 3338 35 35
DraI TTT'AAA
Cuts at: 2538 3306 4074 4817 2538
Size: 768 768 743 5234
Fragments arranged by size:
5234 768 768 743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6520 809
Size: 507 5204 1802
Fragments arranged by size:
5204 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2237 4960 5849
Size: 54 1425 50 275 18 407 2723 889
Cuts at: 5849 7478 8
Size: 1629 43
Fragments arranged by size:
2723 1629 1425 889 407 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4960 1537
Size: 275 3148 4090
Fragments arranged by size:
4090 3148 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4515 5443 1353
Size: 3162 928 3423
Fragments arranged by size:
3423 3162 928
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6100 6474 6620 387
Size: 1064 138 4511 374 146 1280
Fragments arranged by size:
4511 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2921 3060 3651 4827 5576 6173
Size: 393 461 828 139 591 1176 749 597
Cuts at: 6173 6960 7228 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1176 828 787 749 597 591 461
393 268 139
EcoNI CCTnn'n_nnAGG
Cuts at: 2804 2804
Size: 7513
EcoO109I rG'GnC_Cy
Cuts at: 2087 2087
Size: 7513
EcoRI G'AATT_C
Cuts at: 2662 2786 4822 5200 2662
Size: 124 2036 378 4975
Fragments arranged by size:
4975 2036 378 124
EcoRV GAT'ATC
Cuts at: 3552 4189 3552
Size: 637 6876
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2340 3146 2340
Size: 806 6707
FspI TGC'GCA
Cuts at: 6019 6019
Size: 7513
HaeII r_GCGC'y
Cuts at: 1678 6660 1678
Size: 4982 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2798 2830 2933
Size: 32 135 32 437 32 757 32 103
Cuts at: 2933 2965 3603 3635 3839 3871 4541 4573 6154
Size: 32 638 32 204 32 670 32 1581
Cuts at: 6154 6186 6228 6260 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1581 757 670 638 437 204 135
103 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3100 4857 5103 669
Size: 988 281 1162 1757 246 3079
Fragments arranged by size:
3079 1757 1162 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7513
HpaI GTT'AAC
Cuts at: 3100 5103 3100
Size: 2003 5510
KpnI G_GTAC'C
Cuts at: 4838 4838
Size: 7513
MfeI C'AATT_G
Cuts at: 4572 4712 5112 4572
Size: 140 400 6973
Fragments arranged by size:
6973 400 140
MluI A'CGCG_T
Cuts at: 7389 7389
Size: 7513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6627 6811 1305
Size: 5322 184 2007
Fragments arranged by size:
5322 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5691
Fragments arranged by size:
5691 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3623 4404 5471 5830 5989 518
Size: 648 2457 781 1067 359 159 2042
Fragments arranged by size:
2457 2042 1067 781 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2837 4929 5606 6754 6999 1606
Size: 1231 2092 677 1148 245 2120
Fragments arranged by size:
2120 2092 1231 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6961
NdeI CA'TA_TG
Cuts at: 387 2584 4117 387
Size: 2197 1533 3783
Fragments arranged by size:
3783 2197 1533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7513
NotI GC'GGCC_GC
Cuts at: 4960 4960
Size: 7513
NsiI A_TGCA'T
Cuts at: 2780 4437 2780
Size: 1657 5856
NspI r_CATG'y
Cuts at: 1132 1229 4435 4454 4871 6416 1132
Size: 97 3206 19 417 1545 2229
Fragments arranged by size:
3206 2229 1545 417 97 19
PacI TTA_AT'TAA
Cuts at: 2976 3729 2976
Size: 753 6760
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7513
PciI A'CATG_T
Cuts at: 4450 6412 4450
Size: 1962 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7513
PfoI T'CCnGG_A
Cuts at: 2109 3037 4386 2109
Size: 928 1349 5236
Fragments arranged by size:
5236 1349 928
PmeI GTTT'AAAC
Cuts at: 4817 4817
Size: 7513
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5582 5614 7095 7127 5582
Size: 32 1481 32 5968
Fragments arranged by size:
5968 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2087 2087
Size: 7513
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7513
PsiI TTA'TAA
Cuts at: 2400 5083 2400
Size: 2683 4830
PspXI vC'TCGA_Gb
Cuts at: 3606 3606
Size: 7513
PstI C_TGCA'G
Cuts at: 830 4549 4865 830
Size: 3719 316 3478
Fragments arranged by size:
3719 3478 316
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7513
PvuII CAG'CTG
Cuts at: 2837 2837
Size: 7513
SalI G'TCGA_C
Cuts at: 1936 4855 1936
Size: 2919 4594
SanDI GG'GwC_CC
Cuts at: 2087 2087
Size: 7513
SbfI CC_TGCA'GG
Cuts at: 4865 4865
Size: 7513
ScaI AGT'ACT
Cuts at: 4177 5761 7473 4177
Size: 1584 1712 4217
Fragments arranged by size:
4217 1712 1584
SfcI C'TryA_G
Cuts at: 826 1045 2989 3630 4545 4861 5996 6677 6868
Size: 219 1944 641 915 316 1135 681 191
Cuts at: 6868 826
Size: 1471
Fragments arranged by size:
1944 1471 1135 915 681 641 316 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7513
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7513
SmaI CCC'GGG
Cuts at: 1872 4840 1872
Size: 2968 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2244 3606 4465 4751 5617 6518
Size: 197 926 301 1362 859 286 866 901
Cuts at: 6518 6780 820
Size: 262 1553
Fragments arranged by size:
1553 1362 926 901 866 859 301 286
262 197
SnaBI TAC'GTA
Cuts at: 493 5304 493
Size: 4811 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7513
SphI G_CATG'C
Cuts at: 1132 4871 1132
Size: 3739 3774
SspI AAT'ATT
Cuts at: 5 52 4133 5437 5
Size: 47 4081 1304 2081
Fragments arranged by size:
4081 2081 1304 47
StuI AGG'CCT
Cuts at: 1517 2014 1517
Size: 497 7016
StyI C'CwwG_G
Cuts at: 513 1065 3285 3360 4587 4952 513
Size: 552 2220 75 1227 365 3074
Fragments arranged by size:
3074 2220 1227 552 365 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2125 5540 5557 5710 5895 6340 325
Size: 1255 545 3415 17 153 185 445 1498
Fragments arranged by size:
3415 1498 1255 545 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2760 4175 4761
Size: 274 80 33 51 1443 783 1415 586
Cuts at: 4761 5759 7471 96
Size: 998 1712 138
Fragments arranged by size:
1712 1443 1415 998 783 586 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7473 7505 7473
Size: 32 7481
Tth111I GACn'n_nGTC
Cuts at: 1316 3233 1316
Size: 1917 5596
XbaI T'CTAG_A
Cuts at: 4849 4849
Size: 7513
XhoI C'TCGA_G
Cuts at: 1943 3606 1943
Size: 1663 5850
XmaI C'CCGG_G
Cuts at: 1870 4838 1870
Size: 2968 4545
XmnI GAAnn'nnTTC
Cuts at: 2735 2868 3129 3822 4773 4791 5642 2735
Size: 133 261 693 951 18 851 4606
Fragments arranged by size:
4606 951 851 693 261 133 18
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5321 276
Size: 53 83 186 936 3787 2468
Fragments arranged by size:
3787 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PmlI PspOMI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI