Restriction map for
pFN21AB8499
(Circular) (Six-base) MAPSORT of: pFN21AB8499.seq Check: 6773 from: 1 to: 7501
pFN21AB8499 7501 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5311 278
Size: 53 83 186 936 3775 2468
Fragments arranged by size:
3775 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2797 4609 4844 1937
Size: 860 1812 235 4594
Fragments arranged by size:
4594 1812 860 235
Acc65I G'GTAC_C
Cuts at: 4822 4822
Size: 7501
AclI AA'CG_TT
Cuts at: 1563 5628 6001 1563
Size: 4065 373 3063
Fragments arranged by size:
4065 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2712 3144 4034 5564 6948 7216 1632
Size: 1080 432 890 1530 1384 268 1917
Fragments arranged by size:
1917 1530 1384 1080 890 432 268
AflII C'TTAA_G
Cuts at: 820 1017 2560 820
Size: 197 1543 5761
Fragments arranged by size:
5761 1543 197
AflIII A'CryG_T
Cuts at: 1203 6400 7377 1203
Size: 5197 977 1327
Fragments arranged by size:
5197 1327 977
AgeI A'CCGG_T
Cuts at: 6332 6332
Size: 7501
AhdI GACnn_n'nnGTC
Cuts at: 6230 6230
Size: 7501
AleI CACnn'nnGTG
Cuts at: 1166 2063 1166
Size: 897 6604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7501
AlwNI CAG_nnn'CTG
Cuts at: 5244 6816 5244
Size: 1572 5929
ApaLI G'TGCA_C
Cuts at: 5558 6714 5558
Size: 1156 6345
ApoI r'AATT_y
Cuts at: 1493 2260 2929 3034 3276 3480 3520 3552 3985
Size: 767 669 105 242 204 40 32 433
Cuts at: 3985 4129 4560 4810 5036 5188 7297 1493
Size: 144 431 250 226 152 2109 1697
Fragments arranged by size:
2109 1697 767 669 433 431 250 242
226 204 152 144 105 40 32
AseI AT'TA_AT
Cuts at: 160 1032 6055 160
Size: 872 5023 1606
Fragments arranged by size:
5023 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7501
AvaI C'yCGr_G
Cuts at: 1870 1943 4826 1870
Size: 73 2883 4545
Fragments arranged by size:
4545 2883 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2415 2448 3147 3180 3886 3919 2415
Size: 33 699 33 706 33 5997
Fragments arranged by size:
5997 706 699 33 33 33
BamHI G'GATC_C
Cuts at: 4831 5198 4831
Size: 367 7134
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 4822 6277 618
Size: 325 217 627 324 2711 1455 1842
Fragments arranged by size:
2711 1842 1455 627 325 324 217
BanII G_rGCy'C
Cuts at: 1391 4820 1391
Size: 3429 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3839 928
Size: 971 1940 4590
Fragments arranged by size:
4590 1940 971
BbvCI CC'TCA_GC
Cuts at: 2316 2316
Size: 7501
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5692 5726 1903
Size: 34 3755 34 3678
Fragments arranged by size:
3755 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2555 2889 5393 6603 2555
Size: 334 2504 1210 3453
Fragments arranged by size:
3453 2504 1210 334
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7501
BfrBI ATG'CAT
Cuts at: 3187 3308 4208 3187
Size: 121 900 6480
Fragments arranged by size:
6480 900 121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6112 136
Size: 107 122 71 631 605 4440 1525
Fragments arranged by size:
4440 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2251 2524 7496 2251
Size: 273 4972 2256
Fragments arranged by size:
4972 2256 273
BlpI GC'TnA_GC
Cuts at: 4918 4918
Size: 7501
Bme1580I G_kGCm'C
Cuts at: 1790 5562 6718 1790
Size: 3772 1156 2573
Fragments arranged by size:
3772 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7501
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2356 3394 5232
Size: 416 53 83 399 24 939 1038 1838
Cuts at: 5232 6190 442
Size: 958 1753
Fragments arranged by size:
1838 1753 1038 958 939 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 6161 1239
Size: 917 4005 2579
Fragments arranged by size:
4005 2579 917
Bpu10I CC'TnA_GC
Cuts at: 2316 2316
Size: 7501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4310 5626 6491 6789 4310
Size: 1316 865 298 5022
Fragments arranged by size:
5022 1316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6164 882
Size: 617 4665 2219
Fragments arranged by size:
4665 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5292 493
Size: 711 4088 2702
Fragments arranged by size:
4088 2702 711
BsaBI GATnn'nnATC
Cuts at: 7495 7495
Size: 7501
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5308 5690 6325 275
Size: 53 83 186 936 3775 382 635 1451
Fragments arranged by size:
3775 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5934 6332 6606 6753 7164 5934
Size: 398 274 147 411 6271
Fragments arranged by size:
6271 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4632 4662 1178
Size: 30 165 30 3229 30 4017
Fragments arranged by size:
4017 3229 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 1186
Size: 1333 6168
BseYI C'CCAG_C
Cuts at: 6704 7200 6704
Size: 496 7005
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6928
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4951 5712 6740 1540
Size: 275 186 2950 761 1028 2301
Fragments arranged by size:
2950 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3742 5562 5647 6718 3742
Size: 1820 85 1071 4525
Fragments arranged by size:
4525 1820 1071 85
BsmI GAATG_Cn'
Cuts at: 3310 5012 5105 3310
Size: 1702 93 5706
Fragments arranged by size:
5706 1702 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3742 4820 5562 5647 6718 1391
Size: 399 1952 1078 742 85 1071 2174
Fragments arranged by size:
2174 1952 1078 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7164 7164
Size: 7501
BspHI T'CATG_A
Cuts at: 2245 2532 5390 7120 2245
Size: 287 2858 1730 2626
Fragments arranged by size:
2858 2626 1730 287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3449 4856 844
Size: 2605 1407 3489
Fragments arranged by size:
3489 2605 1407
BsrBI CCG'CTC
Cuts at: 1394 1823 5388 1394
Size: 429 3565 3507
Fragments arranged by size:
3565 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2220 5996 6170 65
Size: 1183 972 3776 174 1396
Fragments arranged by size:
3776 1396 1183 972 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6145 6332 1700
Size: 110 4335 187 2869
Fragments arranged by size:
4335 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2575 96
Size: 2479 5022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7501
BssSI C'ACGA_G
Cuts at: 5561 6573 5561
Size: 1012 6489
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7501
BstBI TT'CG_AA
Cuts at: 6305 6305
Size: 7501
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7501
BstYI r'GATC_y
Cuts at: 1972 2251 2517 2524 4098 4358 4831 5198 5583
Size: 279 266 7 1574 260 473 367 385
Cuts at: 5583 5600 7052 7138 7150 7496 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1574 1452 473 385 367 346 279
266 260 86 17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6949
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6762
Fragments arranged by size:
6762 683 56
BtsI GCAGTG_nn'
Cuts at: 2386 4653 5017 5811 5831 2386
Size: 2267 364 794 20 4056
Fragments arranged by size:
4056 2267 794 364 20
ClaI AT'CG_AT
Cuts at: 1554 2901 1554
Size: 1347 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7466
DraI TTT'AAA
Cuts at: 3225 4279 4554 4805 3225
Size: 1054 275 251 5921
Fragments arranged by size:
5921 1054 275 251
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6508 809
Size: 507 5192 1802
Fragments arranged by size:
5192 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2383 4948 5837
Size: 54 1425 50 275 18 553 2565 889
Cuts at: 5837 7466 8
Size: 1629 43
Fragments arranged by size:
2565 1629 1425 889 553 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4948 1537
Size: 275 3136 4090
Fragments arranged by size:
4090 3136 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3478 3840 3861 3905 4155 5431 1353
Size: 2125 362 21 44 250 1276 3423
Fragments arranged by size:
3423 2125 1276 362 250 44 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6088 6462 6608 387
Size: 1064 138 4499 374 146 1280
Fragments arranged by size:
4499 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2712 3144 4034 5564 6161 6948
Size: 393 524 556 432 890 1530 597 787
Cuts at: 6948 7216 1239
Size: 268 1524
Fragments arranged by size:
1530 1524 890 787 597 556 524 432
393 268
EcoRI G'AATT_C
Cuts at: 4810 5188 4810
Size: 378 7123
EcoRV GAT'ATC
Cuts at: 2818 2818
Size: 7501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3479 4580 3479
Size: 1101 6400
FspI TGC'GCA
Cuts at: 6007 6007
Size: 7501
HaeII r_GCGC'y
Cuts at: 1678 6648 1678
Size: 4970 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2509 2541 3656 3688 4174
Size: 32 135 32 937 32 1115 32 486
Cuts at: 4174 4206 6142 6174 6216 6248 1373
Size: 32 1936 32 42 32 2626
Fragments arranged by size:
2626 1936 1115 937 486 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2798 4153 4338 4845 5091 669
Size: 988 281 860 1355 185 507 246 3079
Fragments arranged by size:
3079 1355 988 860 507 281 246 185
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7501
HpaI GTT'AAC
Cuts at: 4338 5091 4338
Size: 753 6748
KpnI G_GTAC'C
Cuts at: 4826 4826
Size: 7501
MfeI C'AATT_G
Cuts at: 2012 5100 2012
Size: 3088 4413
MluI A'CGCG_T
Cuts at: 7377 7377
Size: 7501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2857 6615 6799 1305
Size: 1552 3758 184 2007
Fragments arranged by size:
3758 2007 1552 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2385 10
Size: 54 1425 343 553 5126
Fragments arranged by size:
5126 1425 553 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 3192 3323 3429 5459 5818 5977
Size: 648 897 1129 131 106 2030 359 159
Cuts at: 5977 518
Size: 2042
Fragments arranged by size:
2042 2030 1129 897 648 359 159 131
106
MspA1I CmG'CkG
Cuts at: 1606 2915 3071 4917 5594 6742 6987 1606
Size: 1309 156 1846 677 1148 245 2120
Fragments arranged by size:
2120 1846 1309 1148 677 245 156
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6949
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7501
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7501
NotI GC'GGCC_GC
Cuts at: 4948 4948
Size: 7501
NsiI A_TGCA'T
Cuts at: 3189 3310 4210 3189
Size: 121 900 6480
Fragments arranged by size:
6480 900 121
NspI r_CATG'y
Cuts at: 1132 1229 4859 6404 1132
Size: 97 3630 1545 2229
Fragments arranged by size:
3630 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7501
PciI A'CATG_T
Cuts at: 6400 6400
Size: 7501
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3981 4538 1108
Size: 2873 557 4071
Fragments arranged by size:
4071 2873 557
PmeI GTTT'AAAC
Cuts at: 4805 4805
Size: 7501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5570 5602 7083 7115 5570
Size: 32 1481 32 5956
Fragments arranged by size:
5956 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7501
PsiI TTA'TAA
Cuts at: 3380 5071 3380
Size: 1691 5810
PstI C_TGCA'G
Cuts at: 830 3490 4312 4853 830
Size: 2660 822 541 3478
Fragments arranged by size:
3478 2660 822 541
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7501
PvuII CAG'CTG
Cuts at: 2915 3071 2915
Size: 156 7345
SalI G'TCGA_C
Cuts at: 1936 2796 4843 1936
Size: 860 2047 4594
Fragments arranged by size:
4594 2047 860
SbfI CC_TGCA'GG
Cuts at: 4853 4853
Size: 7501
ScaI AGT'ACT
Cuts at: 2222 5749 7461 2222
Size: 3527 1712 2262
Fragments arranged by size:
3527 2262 1712
SfcI C'TryA_G
Cuts at: 826 1045 2910 3486 4308 4708 4797 4849 5984
Size: 219 1865 576 822 400 89 52 1135
Cuts at: 5984 6665 6856 826
Size: 681 191 1471
Fragments arranged by size:
1865 1471 1135 822 681 576 400 219
191 89 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7501
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7501
SmaI CCC'GGG
Cuts at: 1872 4828 1872
Size: 2956 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2560 4289 5605 6506 6768 820
Size: 197 926 617 1729 1316 901 262 1553
Fragments arranged by size:
1729 1553 1316 926 901 617 262 197
SnaBI TAC'GTA
Cuts at: 493 5292 493
Size: 4799 2702
SpeI A'CTAG_T
Cuts at: 152 2753 3791 152
Size: 2601 1038 3862
Fragments arranged by size:
3862 2601 1038
SphI G_CATG'C
Cuts at: 1132 4859 1132
Size: 3727 3774
SspI AAT'ATT
Cuts at: 5 52 3538 4084 5425 5
Size: 47 3486 546 1341 2081
Fragments arranged by size:
3486 2081 1341 546 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7501
StyI C'CwwG_G
Cuts at: 513 1065 2100 2292 4940 513
Size: 552 1035 192 2648 3074
Fragments arranged by size:
3074 2648 1035 552 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2375 3413 5528 5545 5698 5883 6328
Size: 1255 795 1038 2115 17 153 185 445
Cuts at: 6328 325
Size: 1498
Fragments arranged by size:
2115 1498 1255 1038 795 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 2575 2803
Size: 274 80 33 51 1443 243 355 228
Cuts at: 2803 3029 4674 5747 7459 96
Size: 226 1645 1073 1712 138
Fragments arranged by size:
1712 1645 1443 1073 355 274 243 228
226 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4632 4664 7461 7493 4632
Size: 32 2797 32 4640
Fragments arranged by size:
4640 2797 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7501
XbaI T'CTAG_A
Cuts at: 4837 4837
Size: 7501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3239 3407 4409 3239
Size: 168 1002 6331
Fragments arranged by size:
6331 1002 168
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7501
XmaI C'CCGG_G
Cuts at: 1870 4826 1870
Size: 2956 4545
XmnI GAAnn'nnTTC
Cuts at: 2676 4047 4454 5630 2676
Size: 1371 407 1176 4547
Fragments arranged by size:
4547 1371 1176 407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5309 276
Size: 53 83 186 936 3775 2468
Fragments arranged by size:
3775 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI