Restriction map for
pFN21AB8492
(Circular) (Six-base) MAPSORT of: pFN21AB8492.seq Check: 1719 from: 1 to: 7279
pFN21AB8492 7279 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3084 4255 3084
Size: 1171 6108
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4116 4125 5089 278
Size: 53 83 186 936 2580 9 964 2468
Fragments arranged by size:
2580 2468 964 936 186 83 53 9
AccI GT'mk_AC
Cuts at: 1937 2022 2331 4622 1937
Size: 85 309 2291 4594
Fragments arranged by size:
4594 2291 309 85
Acc65I G'GTAC_C
Cuts at: 2633 4600 2633
Size: 1967 5312
AclI AA'CG_TT
Cuts at: 1563 5406 5779 1563
Size: 3843 373 3063
Fragments arranged by size:
3843 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2699 3032 3299 3356 3596 3698 5342 6726
Size: 1067 333 267 57 240 102 1644 1384
Cuts at: 6726 6994 1632
Size: 268 1917
Fragments arranged by size:
1917 1644 1384 1067 333 268 267 240
102 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7082
AflIII A'CryG_T
Cuts at: 1203 2067 4311 6178 7155 1203
Size: 864 2244 1867 977 1327
Fragments arranged by size:
2244 1867 1327 977 864
AgeI A'CCGG_T
Cuts at: 6110 6110
Size: 7279
AhdI GACnn_n'nnGTC
Cuts at: 2398 4405 6008 2398
Size: 2007 1603 3669
Fragments arranged by size:
3669 2007 1603
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2460 3023 4201 1184
Size: 1276 563 1178 4262
Fragments arranged by size:
4262 1276 1178 563
AlwNI CAG_nnn'CTG
Cuts at: 3439 4462 5022 6594 3439
Size: 1023 560 1572 4124
Fragments arranged by size:
4124 1572 1023 560
ApaI G_GGCC'C
Cuts at: 3132 3300 3966 3132
Size: 168 666 6445
Fragments arranged by size:
6445 666 168
ApaLI G'TGCA_C
Cuts at: 5336 6492 5336
Size: 1156 6123
ApoI r'AATT_y
Cuts at: 1493 2920 4588 4814 4966 7075 1493
Size: 1427 1668 226 152 2109 1697
Fragments arranged by size:
2109 1697 1668 1427 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5833 160
Size: 872 4801 1606
Fragments arranged by size:
4801 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 2321 2516 3256 4604 1870
Size: 73 208 170 195 740 1348 4545
Fragments arranged by size:
4545 1348 740 208 195 170 73
BamHI G'GATC_C
Cuts at: 4609 4976 4609
Size: 367 6912
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2288 2633 3208 3452 3647
Size: 325 217 627 501 345 575 244 195
Cuts at: 3647 3971 4337 4600 6055 618
Size: 324 366 263 1455 1842
Fragments arranged by size:
1842 1455 627 575 501 366 345 325
324 263 244 217 195
BanII G_rGCy'C
Cuts at: 1391 2247 2517 2526 3117 3132 3300 3384 3888
Size: 856 270 9 591 15 168 84 504
Cuts at: 3888 3966 4233 4598 1391
Size: 78 267 365 4072
Fragments arranged by size:
4072 856 591 504 365 270 267 168
84 78 15 9
BbeI G_GCGC'C
Cuts at: 3651 3651
Size: 7279
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3244 3452 928
Size: 971 1345 208 4755
Fragments arranged by size:
4755 1345 971 208
BbvCI CC'TCA_GC
Cuts at: 3936 3936
Size: 7279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3128 3162 5470 5504 1903
Size: 34 1191 34 2308 34 3678
Fragments arranged by size:
3678 2308 1191 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5171 6381 5171
Size: 1210 6069
BclI T'GATC_A
Cuts at: 1548 3063 1548
Size: 1515 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2604 2750 3423
Size: 107 122 71 631 605 932 146 673
Cuts at: 3423 3533 5890 136
Size: 110 2357 1525
Fragments arranged by size:
2357 1525 932 673 631 605 146 122
110 107 71
BglII A'GATC_T
Cuts at: 3669 3948 7274 3669
Size: 279 3326 3674
Fragments arranged by size:
3674 3326 279
BlpI GC'TnA_GC
Cuts at: 4696 4696
Size: 7279
Bme1580I G_kGCm'C
Cuts at: 1790 3132 3300 3455 3966 5340 6496 1790
Size: 1342 168 155 511 1374 1156 2573
Fragments arranged by size:
2573 1374 1342 1156 511 168 155
BmgBI CAC'GTC
Cuts at: 1318 3931 1318
Size: 2613 4666
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4429 5010 5968
Size: 416 53 83 399 24 3012 581 958
Cuts at: 5968 442
Size: 1753
Fragments arranged by size:
3012 1753 958 581 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 2531 3686 3707 5939 1239
Size: 1280 12 1155 21 2232 2579
Fragments arranged by size:
2579 2232 1280 1155 21 12
Bpu10I CC'TnA_GC
Cuts at: 3936 3936
Size: 7279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3872 5404 6269 6567 3872
Size: 1532 865 298 4584
Fragments arranged by size:
4584 1532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3271 4464 4508 5942 882
Size: 617 1772 1193 44 1434 2219
Fragments arranged by size:
2219 1772 1434 1193 617 44
BsaAI yAC'GTr
Cuts at: 493 1204 2086 3376 4312 5070 493
Size: 711 882 1290 936 758 2702
Fragments arranged by size:
2702 1290 936 882 758 711
BsaBI GATnn'nnATC
Cuts at: 3068 7273 3068
Size: 4205 3074
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3048 3648 4113 4122
Size: 53 83 186 936 1515 600 465 9
Cuts at: 4122 4140 5086 5468 6103 275
Size: 18 946 382 635 1451
Fragments arranged by size:
1515 1451 946 936 635 600 465 382
186 83 53 18 9
BsaWI w'CCGG_w
Cuts at: 5712 6110 6384 6531 6942 5712
Size: 398 274 147 411 6049
Fragments arranged by size:
6049 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2717 2747 3065 3095 4238
Size: 30 165 30 1314 30 318 30 1143
Cuts at: 4238 4268 1178
Size: 30 4189
Fragments arranged by size:
4189 1314 1143 318 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2329 2534 2948 3635 3704 4100 4334 1186
Size: 1143 205 414 687 69 396 234 4131
Fragments arranged by size:
4131 1143 687 414 396 234 205 69
BseYI C'CCAG_C
Cuts at: 2806 4212 4496 6482 6978 2806
Size: 1406 284 1986 496 3107
Fragments arranged by size:
3107 1986 1406 496 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 3626 3644 4031 4115 1163
Size: 573 699 1191 18 387 84 4327
Fragments arranged by size:
4327 1191 699 573 387 84 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3732 4729 5490 6518 1540
Size: 275 186 1731 997 761 1028 2301
Fragments arranged by size:
2301 1731 1028 997 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 2526 3117 3474 3774 3888 5340 5425 6496
Size: 279 591 357 300 114 1452 85 1071
Cuts at: 6496 2247
Size: 3030
Fragments arranged by size:
3030 1452 1071 591 357 300 279 114
85
BsmI GAATG_Cn'
Cuts at: 4790 4883 4790
Size: 93 7186
BsmBI CGTCTCn'nnnn_
Cuts at: 3039 3739 3740 3039
Size: 700 1 6578
Fragments arranged by size:
6578 700 1
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2517 2526 3117 3132 3300 3384
Size: 399 457 270 9 591 15 168 84
Cuts at: 3384 3455 3474 3774 3888 3966 4233 4598 5340
Size: 71 19 300 114 78 267 365 742
Cuts at: 5340 5425 6496 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 591 457 399 365 300
270 267 168 114 85 84 78 71
19 15 9
BspEI T'CCGG_A
Cuts at: 6942 6942
Size: 7279
BspHI T'CATG_A
Cuts at: 2613 2916 5168 6898 2613
Size: 303 2252 1730 2994
Fragments arranged by size:
2994 2252 1730 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3084 3561 4255 4634 844
Size: 2240 477 694 379 3489
Fragments arranged by size:
3489 2240 694 477 379
BsrBI CCG'CTC
Cuts at: 1394 1823 2595 2749 5166 1394
Size: 429 772 154 2417 3507
Fragments arranged by size:
3507 2417 772 429 154
BsrDI GCAATG_nn'
Cuts at: 65 1248 2442 5774 5948 65
Size: 1183 1194 3332 174 1396
Fragments arranged by size:
3332 1396 1194 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2291 3650 3804 3974 4046 4334 5923
Size: 110 481 1359 154 170 72 288 1589
Cuts at: 5923 6110 1700
Size: 187 2869
Fragments arranged by size:
2869 1589 1359 481 288 187 170 154
110 72
BsrGI T'GTAC_A
Cuts at: 96 3810 96
Size: 3714 3565
BssHII G'CGCG_C
Cuts at: 1925 3899 1925
Size: 1974 5305
BssSI C'ACGA_G
Cuts at: 3419 5339 6351 3419
Size: 1920 1012 4347
Fragments arranged by size:
4347 1920 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2871 3484 4462 794
Size: 2077 613 978 3611
Fragments arranged by size:
3611 2077 978 613
BstBI TT'CG_AA
Cuts at: 6083 6083
Size: 7279
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7279
BstXI CCAn_nnnn'nTGG
Cuts at: 3565 4351 4504 3565
Size: 786 153 6340
Fragments arranged by size:
6340 786 153
BstYI r'GATC_y
Cuts at: 1972 2028 3669 3948 4248 4458 4609 4976 5361
Size: 56 1641 279 300 210 151 367 385
Cuts at: 5361 5378 6830 6916 6928 7274 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1641 1452 385 367 346 300 279
210 151 86 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 4403 4403
Size: 7279
BtgI C'CryG_G
Cuts at: 513 1065 2004 2092 2601 2637 2659 3150 3528
Size: 552 939 88 509 36 22 491 378
Cuts at: 3528 3600 4150 513
Size: 72 550 3642
Fragments arranged by size:
3642 939 552 550 509 491 378 88
72 36 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2062 2125 3625 487
Size: 683 56 836 63 1500 4141
Fragments arranged by size:
4141 1500 836 683 63 56
BtsI GCAGTG_nn'
Cuts at: 3614 4795 5589 5609 3614
Size: 1181 794 20 5284
Fragments arranged by size:
5284 1181 794 20
ClaI AT'CG_AT
Cuts at: 1554 2619 3855 1554
Size: 1065 1236 4978
Fragments arranged by size:
4978 1236 1065
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7244
DraI TTT'AAA
Cuts at: 4583 4583
Size: 7279
DraIII CAC_nnn'GTG
Cuts at: 4082 4082
Size: 7279
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3731 6286 809
Size: 507 2415 2555 1802
Fragments arranged by size:
2555 2415 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2703 3525 3585
Size: 54 1425 50 275 18 873 822 60
Cuts at: 3585 3843 3909 4044 4332 4420 4726 5615 7244
Size: 258 66 135 288 88 306 889 1629
Cuts at: 7244 8
Size: 43
Fragments arranged by size:
1629 1425 889 873 822 306 288 275
258 135 88 66 60 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4726 1537
Size: 275 2914 4090
Fragments arranged by size:
4090 2914 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 3753 5209 1353
Size: 887 1513 1456 3423
Fragments arranged by size:
3423 1513 1456 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3862 4526 5866 6240 6386 387
Size: 1064 138 2273 664 1340 374 146 1280
Fragments arranged by size:
2273 1340 1280 1064 664 374 146 138
EcoICRI GAG'CTC
Cuts at: 2245 2524 3115 3886 2245
Size: 279 591 771 5638
Fragments arranged by size:
5638 771 591 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2531 2699 3032 3299 3356 3596
Size: 393 887 12 168 333 267 57 240
Cuts at: 3596 3686 3698 3707 5342 5939 6726 6994 1239
Size: 90 12 9 1635 597 787 268 1524
Fragments arranged by size:
1635 1524 887 787 597 393 333 268
267 240 168 90 57 12 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3011 3122 3689 3845 3011
Size: 111 567 156 6445
Fragments arranged by size:
6445 567 156 111
EcoO109I rG'GnC_Cy
Cuts at: 2738 3128 3129 3179 3296 3325 3682 4531 2738
Size: 390 1 50 117 29 357 849 5486
Fragments arranged by size:
5486 849 390 357 117 50 29 1
EcoRI G'AATT_C
Cuts at: 4588 4966 4588
Size: 378 6901
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3200 3200
Size: 7279
FspI TGC'GCA
Cuts at: 5785 5785
Size: 7279
HaeII r_GCGC'y
Cuts at: 1678 3651 4186 6426 1678
Size: 1973 535 2240 2531
Fragments arranged by size:
2531 2240 1973 535
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 2384 2416 3621
Size: 32 135 32 752 32 28 32 1205
Cuts at: 3621 3653 4240 4272 4391 4423 5920 5952 5994
Size: 32 587 32 119 32 1497 32 42
Cuts at: 5994 6026 1373
Size: 32 2626
Fragments arranged by size:
2626 1497 1205 752 587 135 119 42
32 32 32 32 32 32 32 32
32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2220 2332 4375 4623 4869 669
Size: 988 281 282 112 2043 248 246 3079
Fragments arranged by size:
3079 2043 988 282 281 248 246 112
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7279
HpaI GTT'AAC
Cuts at: 4869 4869
Size: 7279
KasI G'GCGC_C
Cuts at: 3647 3647
Size: 7279
KpnI G_GTAC'C
Cuts at: 2637 4604 2637
Size: 1967 5312
MfeI C'AATT_G
Cuts at: 4878 4878
Size: 7279
MluI A'CGCG_T
Cuts at: 7155 7155
Size: 7279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3243 3618 4434 6393 6577 1305
Size: 1938 375 816 1959 184 2007
Fragments arranged by size:
2007 1959 1938 816 375 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2705 3587 10
Size: 54 1425 343 873 882 3702
Fragments arranged by size:
3702 1425 882 873 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3488 4343 4349 5237 5596 5755 518
Size: 648 2322 855 6 888 359 159 2042
Fragments arranged by size:
2322 2042 888 855 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2509 2878 3030 3108 3895 4152 4397 4695
Size: 903 369 152 78 787 257 245 298
Cuts at: 4695 5372 6520 6765 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 903 787 677 369 298 257
245 245 152 78
NaeI GCC'GGC
Cuts at: 2293 3652 4048 4336 2293
Size: 1359 396 288 5236
Fragments arranged by size:
5236 1359 396 288
NarI GG'CG_CC
Cuts at: 3648 3648
Size: 7279
NcoI C'CATG_G
Cuts at: 513 1065 2004 2092 2601 3150 3600 513
Size: 552 939 88 509 549 450 4192
Fragments arranged by size:
4192 939 552 549 509 450 88
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7279
NgoMIV G'CCGG_C
Cuts at: 2291 3650 4046 4334 2291
Size: 1359 396 288 5236
Fragments arranged by size:
5236 1359 396 288
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7279
NotI GC'GGCC_GC
Cuts at: 4726 4726
Size: 7279
NspI r_CATG'y
Cuts at: 1132 1229 2071 2330 3487 4096 4637 6182 1132
Size: 97 842 259 1157 609 541 1545 2229
Fragments arranged by size:
2229 1545 1157 842 609 541 259 97
PasI CC'CwG_GG
Cuts at: 1348 3311 1348
Size: 1963 5316
PciI A'CATG_T
Cuts at: 2067 6178 2067
Size: 4111 3168
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3439 4507 1108
Size: 2331 1068 3880
Fragments arranged by size:
3880 2331 1068
PfoI T'CCnGG_A
Cuts at: 2883 4566 2883
Size: 1683 5596
PmeI GTTT'AAAC
Cuts at: 4583 4583
Size: 7279
PmlI CAC'GTG
Cuts at: 2086 4312 2086
Size: 2226 5053
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5348 5380 6861 6893 5348
Size: 32 1481 32 5734
Fragments arranged by size:
5734 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3325 3682 3325
Size: 357 6922
PshAI GACnn'nnGTC
Cuts at: 1613 3225 1613
Size: 1612 5667
PsiI TTA'TAA
Cuts at: 4849 4849
Size: 7279
PspOMI G'GGCC_C
Cuts at: 3128 3296 3962 3128
Size: 168 666 6445
Fragments arranged by size:
6445 666 168
PstI C_TGCA'G
Cuts at: 830 2508 4631 830
Size: 1678 2123 3478
Fragments arranged by size:
3478 2123 1678
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7279
PvuII CAG'CTG
Cuts at: 2509 2878 2509
Size: 369 6910
SacI G_AGCT'C
Cuts at: 2247 2526 3117 3888 2247
Size: 279 591 771 5638
Fragments arranged by size:
5638 771 591 279
SacII CC_GC'GG
Cuts at: 4153 4153
Size: 7279
SalI G'TCGA_C
Cuts at: 1936 2330 4621 1936
Size: 394 2291 4594
Fragments arranged by size:
4594 2291 394
SbfI CC_TGCA'GG
Cuts at: 4631 4631
Size: 7279
ScaI AGT'ACT
Cuts at: 5527 7239 5527
Size: 1712 5567
SfcI C'TryA_G
Cuts at: 826 1045 2504 4627 5762 6443 6634 826
Size: 219 1459 2123 1135 681 191 1471
Fragments arranged by size:
2123 1471 1459 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3533 3533
Size: 7279
SfoI GGC'GCC
Cuts at: 3649 3649
Size: 7279
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
SgrAI Cr'CCGG_yG
Cuts at: 1700 3650 1700
Size: 1950 5329
SmaI CCC'GGG
Cuts at: 1872 2153 2323 4606 1872
Size: 281 170 2283 4545
Fragments arranged by size:
4545 2283 281 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3887 5383 6284 6546 820
Size: 197 926 1944 1496 901 262 1553
Fragments arranged by size:
1944 1553 1496 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5070 493
Size: 4577 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7279
SphI G_CATG'C
Cuts at: 1132 4637 1132
Size: 3505 3774
SrfI GCCC'GGGC
Cuts at: 2153 2153
Size: 7279
SspI AAT'ATT
Cuts at: 5 52 5203 5
Size: 47 5151 2081
Fragments arranged by size:
5151 2081 47
StuI AGG'CCT
Cuts at: 1517 4361 1517
Size: 2844 4435
StyI C'CwwG_G
Cuts at: 513 1065 2004 2092 2601 2718 3150 3600 4002
Size: 552 939 88 509 117 432 450 402
Cuts at: 4002 4065 4718 513
Size: 63 653 3074
Fragments arranged by size:
3074 939 653 552 509 450 432 402
117 88 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5306 5323 5476 5661 6106 325
Size: 1255 3726 17 153 185 445 1498
Fragments arranged by size:
3726 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3810 5525 7237
Size: 274 80 33 51 1443 1833 1715 1712
Cuts at: 7237 96
Size: 138
Fragments arranged by size:
1833 1715 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3065 3097 4238 4270 7239 7271 3065
Size: 32 1141 32 2969 32 3073
Fragments arranged by size:
3073 2969 1141 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3728 1316
Size: 2412 4867
XbaI T'CTAG_A
Cuts at: 4615 4615
Size: 7279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 4240 4504 2090
Size: 2150 264 4865
Fragments arranged by size:
4865 2150 264
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7279
XmaI C'CCGG_G
Cuts at: 1870 2151 2321 4604 1870
Size: 281 170 2283 4545
Fragments arranged by size:
4545 2283 281 170
XmnI GAAnn'nnTTC
Cuts at: 5408 5408
Size: 7279
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4114 4123 5087 276
Size: 53 83 186 936 2580 9 964 2468
Fragments arranged by size:
2580 2468 964 936 186 83 53 9
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SanDI SapI SexAI SwaI