Restriction map for
pFN21AB8490
(Circular) (Six-base) MAPSORT of: pFN21AB8490.seq Check: 5283 from: 1 to: 7282
pFN21AB8490 7282 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2167 2825 3042 2167
Size: 658 217 6407
Fragments arranged by size:
6407 658 217
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4404 5092 278
Size: 53 83 186 936 2868 688 2468
Fragments arranged by size:
2868 2468 936 688 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4404 4625 1937
Size: 2467 221 4594
Fragments arranged by size:
4594 2467 221
Acc65I G'GTAC_C
Cuts at: 4603 4603
Size: 7282
AclI AA'CG_TT
Cuts at: 1563 5409 5782 1563
Size: 3846 373 3063
Fragments arranged by size:
3846 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2355 3342 4565 5345 6729 6997 1632
Size: 723 987 1223 780 1384 268 1917
Fragments arranged by size:
1917 1384 1223 987 780 723 268
AfeI AGC'GCT
Cuts at: 3983 3983
Size: 7282
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7085
AflIII A'CryG_T
Cuts at: 1203 2523 6181 7158 1203
Size: 1320 3658 977 1327
Fragments arranged by size:
3658 1327 1320 977
AgeI A'CCGG_T
Cuts at: 6113 6113
Size: 7282
AhdI GACnn_n'nnGTC
Cuts at: 6011 6011
Size: 7282
AleI CACnn'nnGTG
Cuts at: 1166 3226 1166
Size: 2060 5222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4583 1184
Size: 3399 3883
AlwNI CAG_nnn'CTG
Cuts at: 2046 2088 2194 2227 2320 2788 4082 4163 5025
Size: 42 106 33 93 468 1294 81 862
Cuts at: 5025 6597 2046
Size: 1572 2731
Fragments arranged by size:
2731 1572 1294 862 468 106 93 81
42 33
ApaI G_GGCC'C
Cuts at: 2133 2464 2723 4539 2133
Size: 331 259 1816 4876
Fragments arranged by size:
4876 1816 331 259
ApaLI G'TGCA_C
Cuts at: 5339 6495 5339
Size: 1156 6126
ApoI r'AATT_y
Cuts at: 1493 4409 4451 4591 4817 4969 7078 1493
Size: 2916 42 140 226 152 2109 1697
Fragments arranged by size:
2916 2109 1697 226 152 140 42
AseI AT'TA_AT
Cuts at: 160 1032 5836 160
Size: 872 4804 1606
Fragments arranged by size:
4804 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
AvaI C'yCGr_G
Cuts at: 1870 1943 2716 3314 4607 1870
Size: 73 773 598 1293 4545
Fragments arranged by size:
4545 1293 773 598 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2546 2579 2546
Size: 33 7249
BamHI G'GATC_C
Cuts at: 4612 4979 4612
Size: 367 6915
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2837 3433 4603 6058 618
Size: 325 217 627 1050 596 1170 1455 1842
Fragments arranged by size:
1842 1455 1170 1050 627 596 325 217
BanII G_rGCy'C
Cuts at: 1391 2133 2464 2628 2723 2934 3126 3536 3885
Size: 742 331 164 95 211 192 410 349
Cuts at: 3885 3939 4320 4380 4539 4601 1391
Size: 54 381 60 159 62 4072
Fragments arranged by size:
4072 742 410 381 349 331 211 192
164 159 95 62 60 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2520 4218 4404 928
Size: 971 621 1698 186 3806
Fragments arranged by size:
3806 1698 971 621 186
BbvCI CC'TCA_GC
Cuts at: 2297 3599 2297
Size: 1302 5980
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2697 2731 5473 5507 1903
Size: 34 760 34 2742 34 3678
Fragments arranged by size:
3678 2742 760 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2359 4132 5174 6384 2359
Size: 1773 1042 1210 3257
Fragments arranged by size:
3257 1773 1210 1042
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7282
BfrBI ATG'CAT
Cuts at: 4330 4330
Size: 7282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5893 136
Size: 107 122 71 631 605 4221 1525
Fragments arranged by size:
4221 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4548 7277 4548
Size: 2729 4553
BlpI GC'TnA_GC
Cuts at: 2499 2933 2988 3120 4699 2499
Size: 434 55 132 1579 5082
Fragments arranged by size:
5082 1579 434 132 55
Bme1580I G_kGCm'C
Cuts at: 1790 2133 2464 2575 2723 3327 3399 4170 4462
Size: 343 331 111 148 604 72 771 292
Cuts at: 4462 4539 5343 6499 1790
Size: 77 804 1156 2573
Fragments arranged by size:
2573 1156 804 771 604 343 331 292
148 111 77 72
BmgBI CAC'GTC
Cuts at: 1318 2526 1318
Size: 1208 6074
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2398 3303 5013
Size: 416 53 83 399 24 981 905 1710
Cuts at: 5013 5971 442
Size: 958 1753
Fragments arranged by size:
1753 1710 981 958 905 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2297 3149 3209 3625 4201 4334 5942
Size: 1028 30 852 60 416 576 133 1608
Cuts at: 5942 1239
Size: 2579
Fragments arranged by size:
2579 1608 1028 852 576 416 133 60
30
Bpu10I CC'TnA_GC
Cuts at: 2297 2622 2820 3599 2297
Size: 325 198 779 5980
Fragments arranged by size:
5980 779 325 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3782 3911 4222 4478 4556 5407 6272 6570 3782
Size: 129 311 256 78 851 865 298 4494
Fragments arranged by size:
4494 865 851 311 298 256 129 78
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5945 882
Size: 617 4446 2219
Fragments arranged by size:
4446 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5073 493
Size: 711 3869 2702
Fragments arranged by size:
3869 2702 711
BsaBI GATnn'nnATC
Cuts at: 2582 7276 2582
Size: 4694 2588
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4401 5089 5471 6106
Size: 53 83 186 936 2868 688 382 635
Cuts at: 6106 275
Size: 1451
Fragments arranged by size:
2868 1451 936 688 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4056 5715 6113 6387 6534 6945 4056
Size: 1659 398 274 147 411 4393
Fragments arranged by size:
4393 1659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2991 3021 3623 3653 1178
Size: 30 165 30 1588 30 602 30 4807
Fragments arranged by size:
4807 1588 602 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 2999 3893 3920 1186
Size: 1255 558 894 27 4548
Fragments arranged by size:
4548 1255 894 558 27
BseYI C'CCAG_C
Cuts at: 2207 2247 2307 2434 2445 2533 2747 2797 2927
Size: 40 60 127 11 88 214 50 130
Cuts at: 2927 3093 3132 3170 3399 3553 4531 6485 6981
Size: 166 39 38 229 154 978 1954 496
Cuts at: 6981 2207
Size: 2508
Fragments arranged by size:
2508 1954 978 496 229 214 166 154
130 127 88 60 50 40 39 38
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2180 2195 2636 3230 1163
Size: 573 444 15 441 594 5215
Fragments arranged by size:
5215 594 573 444 441 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2217 4732 5493 6521 1540
Size: 275 186 216 2515 761 1028 2301
Fragments arranged by size:
2515 2301 1028 761 275 216 186
BsiHKAI G_wGCw'C
Cuts at: 2514 2628 3114 3916 3939 4320 5343 5428 6499
Size: 114 486 802 23 381 1023 85 1071
Cuts at: 6499 2514
Size: 3297
Fragments arranged by size:
3297 1071 1023 802 486 381 114 85
23
BsmI GAATG_Cn'
Cuts at: 4793 4886 4793
Size: 93 7189
BsmBI CGTCTCn'nnnn_
Cuts at: 3452 3452
Size: 7282
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2464 2514 2575 2628 2723 2934
Size: 399 343 331 50 61 53 95 211
Cuts at: 2934 3114 3126 3327 3399 3536 3885 3916 3939
Size: 180 12 201 72 137 349 31 23
Cuts at: 3939 4170 4320 4380 4462 4539 4601 5343 5428
Size: 231 150 60 82 77 62 742 85
Cuts at: 5428 6499 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 349 343 331 231
211 201 180 150 137 95 85 82
77 72 62 61 60 53 50 31
23 12
BspEI T'CCGG_A
Cuts at: 6945 6945
Size: 7282
BspHI T'CATG_A
Cuts at: 5171 6901 5171
Size: 1730 5552
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2167 2235 2825 3042 3252 3333 4637 844
Size: 1323 68 590 217 210 81 1304 3489
Fragments arranged by size:
3489 1323 1304 590 217 210 81 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2219 4419 5169 1394
Size: 429 396 2200 750 3507
Fragments arranged by size:
3507 2200 750 429 396
BsrDI GCAATG_nn'
Cuts at: 65 1248 2386 2970 5777 5951 65
Size: 1183 1138 584 2807 174 1396
Fragments arranged by size:
2807 1396 1183 1138 584 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2163 3650 4290 5926 6113 1700
Size: 110 353 1487 640 1636 187 2869
Fragments arranged by size:
2869 1636 1487 640 353 187 110
BsrGI T'GTAC_A
Cuts at: 96 2145 96
Size: 2049 5233
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7282
BssSI C'ACGA_G
Cuts at: 3529 3560 5342 6354 3529
Size: 31 1782 1012 4457
Fragments arranged by size:
4457 1782 1012 31
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2088 2173 2194 2263 2509 4082 4483 794
Size: 1294 85 21 69 246 1573 401 3593
Fragments arranged by size:
3593 1573 1294 401 246 85 69 21
BstBI TT'CG_AA
Cuts at: 6086 6086
Size: 7282
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7282
BstXI CCAn_nnnn'nTGG
Cuts at: 4036 4036
Size: 7282
BstYI r'GATC_y
Cuts at: 1972 4548 4612 4979 5364 5381 6833 6919 6931
Size: 2576 64 367 385 17 1452 86 12
Cuts at: 6931 7277 1972
Size: 346 1977
Fragments arranged by size:
2576 1977 1452 385 367 346 86 64
17 12
Bsu36I CC'TnA_GG
Cuts at: 2488 3619 2488
Size: 1131 6151
BtgI C'CryG_G
Cuts at: 513 1065 2004 2274 2595 3993 513
Size: 552 939 270 321 1398 3802
Fragments arranged by size:
3802 1398 939 552 321 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2479 3631 487
Size: 683 56 1253 1152 4138
Fragments arranged by size:
4138 1253 1152 683 56
BtsI GCAGTG_nn'
Cuts at: 2047 2089 2110 2905 3234 3275 3410 3498 3654
Size: 42 21 795 329 41 135 88 156
Cuts at: 3654 4798 5592 5612 2047
Size: 1144 794 20 3717
Fragments arranged by size:
3717 1144 795 794 329 156 135 88
42 41 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7247
DraI TTT'AAA
Cuts at: 4586 4586
Size: 7282
DraIII CAC_nnn'GTG
Cuts at: 4386 4386
Size: 7282
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6289 809
Size: 507 4973 1802
Fragments arranged by size:
4973 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2214 2598
Size: 54 1425 50 275 18 286 98 384
Cuts at: 2598 3943 4729 5618 7247 8
Size: 1345 786 889 1629 43
Fragments arranged by size:
1629 1425 1345 889 786 384 286 275
98 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2214 4729 1537
Size: 275 402 2515 4090
Fragments arranged by size:
4090 2515 402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5212 1353
Size: 3859 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3767 5869 6243 6389 387
Size: 1064 138 2178 2102 374 146 1280
Fragments arranged by size:
2178 2102 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2626 3937 4318 2626
Size: 1311 381 5590
Fragments arranged by size:
5590 1311 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2297 2355 3149 3209 3342 3625
Size: 393 635 30 58 794 60 133 283
Cuts at: 3625 4201 4334 4565 5345 5942 6729 6997 1239
Size: 576 133 231 780 597 787 268 1524
Fragments arranged by size:
1524 794 787 780 635 597 576 393
283 268 231 133 133 60 58 30
EcoO109I rG'GnC_Cy
Cuts at: 2130 2191 2395 2720 4309 4339 4535 2130
Size: 61 204 325 1589 30 196 4877
Fragments arranged by size:
4877 1589 325 204 196 61 30
EcoRI G'AATT_C
Cuts at: 4409 4451 4591 4969 4409
Size: 42 140 378 6722
Fragments arranged by size:
6722 378 140 42
FspI TGC'GCA
Cuts at: 2391 5788 2391
Size: 3397 3885
HaeII r_GCGC'y
Cuts at: 1678 2282 2307 3985 4518 6429 1678
Size: 604 25 1678 533 1911 2531
Fragments arranged by size:
2531 1911 1678 604 533 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3449 3481 5923 5955 5997
Size: 32 135 32 1877 32 2442 32 42
Cuts at: 5997 6029 1373
Size: 32 2626
Fragments arranged by size:
2626 2442 1877 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4626 4872 669
Size: 988 281 2688 246 3079
Fragments arranged by size:
3079 2688 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7282
HpaI GTT'AAC
Cuts at: 4872 4872
Size: 7282
KpnI G_GTAC'C
Cuts at: 4607 4607
Size: 7282
MfeI C'AATT_G
Cuts at: 4881 4881
Size: 7282
MluI A'CGCG_T
Cuts at: 7158 7158
Size: 7282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6396 6580 1305
Size: 5091 184 2007
Fragments arranged by size:
5091 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5460
Fragments arranged by size:
5460 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3226 3503 4331 4565 5240 5599 5758
Size: 648 2060 277 828 234 675 359 159
Cuts at: 5758 518
Size: 2042
Fragments arranged by size:
2060 2042 828 675 648 359 277 234
159
MspA1I CmG'CkG
Cuts at: 1606 2067 2276 2705 2992 3362 4306 4698 5375
Size: 461 209 429 287 370 944 392 677
Cuts at: 5375 6523 6768 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 944 677 461 429 392 370
287 245 209
NaeI GCC'GGC
Cuts at: 3652 4292 3652
Size: 640 6642
NcoI C'CATG_G
Cuts at: 513 1065 2004 3993 513
Size: 552 939 1989 3802
Fragments arranged by size:
3802 1989 939 552
NdeI CA'TA_TG
Cuts at: 387 4332 387
Size: 3945 3337
NgoMIV G'CCGG_C
Cuts at: 3650 4290 3650
Size: 640 6642
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7282
NotI GC'GGCC_GC
Cuts at: 4729 4729
Size: 7282
NsiI A_TGCA'T
Cuts at: 4332 4332
Size: 7282
NspI r_CATG'y
Cuts at: 1132 1229 3722 4330 4570 4640 6185 1132
Size: 97 2493 608 240 70 1545 2229
Fragments arranged by size:
2493 2229 1545 608 240 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7282
PciI A'CATG_T
Cuts at: 6181 6181
Size: 7282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 4386 1108
Size: 1212 2066 4004
Fragments arranged by size:
4004 2066 1212
PfoI T'CCnGG_A
Cuts at: 3741 3741
Size: 7282
PmeI GTTT'AAAC
Cuts at: 4586 4586
Size: 7282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5351 5383 6864 6896 3617
Size: 32 1702 32 1481 32 4003
Fragments arranged by size:
4003 1702 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4309 4339 4309
Size: 30 7252
PshAI GACnn'nnGTC
Cuts at: 1613 3775 1613
Size: 2162 5120
PsiI TTA'TAA
Cuts at: 4852 4852
Size: 7282
PspOMI G'GGCC_C
Cuts at: 2129 2460 2719 4535 2129
Size: 331 259 1816 4876
Fragments arranged by size:
4876 1816 331 259
PstI C_TGCA'G
Cuts at: 830 2232 2271 2482 2836 2997 3039 3381 4071
Size: 1402 39 211 354 161 42 342 690
Cuts at: 4071 4634 830
Size: 563 3478
Fragments arranged by size:
3478 1402 690 563 354 342 211 161
42 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7282
PvuII CAG'CTG
Cuts at: 2992 3362 2992
Size: 370 6912
SacI G_AGCT'C
Cuts at: 2628 3939 4320 2628
Size: 1311 381 5590
Fragments arranged by size:
5590 1311 381
SacII CC_GC'GG
Cuts at: 2277 2277
Size: 7282
SalI G'TCGA_C
Cuts at: 1936 4624 1936
Size: 2688 4594
SbfI CC_TGCA'GG
Cuts at: 2836 4634 2836
Size: 1798 5484
ScaI AGT'ACT
Cuts at: 5530 7242 5530
Size: 1712 5570
SexAI A'CCwGG_T
Cuts at: 4341 4341
Size: 7282
SfcI C'TryA_G
Cuts at: 826 1045 2228 2267 2478 2832 2993 3035 3377
Size: 219 1183 39 211 354 161 42 342
Cuts at: 3377 4067 4630 5765 6446 6637 826
Size: 690 563 1135 681 191 1471
Fragments arranged by size:
1471 1183 1135 690 681 563 354 342
219 211 191 161 42 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
SgrAI Cr'CCGG_yG
Cuts at: 1700 4290 1700
Size: 2590 4692
SmaI CCC'GGG
Cuts at: 1872 4609 1872
Size: 2737 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3797 3926 4237 4493 4571 5386
Size: 197 926 1854 129 311 256 78 815
Cuts at: 5386 6287 6549 820
Size: 901 262 1553
Fragments arranged by size:
1854 1553 926 901 815 311 262 256
197 129 78
SnaBI TAC'GTA
Cuts at: 493 5073 493
Size: 4580 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7282
SphI G_CATG'C
Cuts at: 1132 4570 4640 1132
Size: 3438 70 3774
Fragments arranged by size:
3774 3438 70
SspI AAT'ATT
Cuts at: 5 52 5206 5
Size: 47 5154 2081
Fragments arranged by size:
5154 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7282
StyI C'CwwG_G
Cuts at: 513 1065 2004 3993 4134 4721 513
Size: 552 939 1989 141 587 3074
Fragments arranged by size:
3074 1989 939 587 552 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3608 4276 5309 5326 5479 5664 6109
Size: 1255 2028 668 1033 17 153 185 445
Cuts at: 6109 325
Size: 1498
Fragments arranged by size:
2028 1498 1255 1033 668 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 5528 7240
Size: 274 80 33 51 1443 168 3383 1712
Cuts at: 7240 96
Size: 138
Fragments arranged by size:
3383 1712 1443 274 168 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2991 3023 3804 3836 7242 7274 2991
Size: 32 781 32 3406 32 2999
Fragments arranged by size:
3406 2999 781 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3463 1316
Size: 2147 5135
XbaI T'CTAG_A
Cuts at: 4618 4618
Size: 7282
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7282
XmaI C'CCGG_G
Cuts at: 1870 4607 1870
Size: 2737 4545
XmnI GAAnn'nnTTC
Cuts at: 2368 5411 2368
Size: 3043 4239
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4402 5090 276
Size: 53 83 186 936 2868 688 2468
Fragments arranged by size:
2868 2468 936 688 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BplI BsiWI BstZ17I EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PmlI PspXI PsrI RsrII SanDI SapI SfiI SfoI
SrfI SwaI XcmI