Restriction map for
pFN21AB8483
(Circular) (Six-base) MAPSORT of: pFN21AB8483.seq Check: 8405 from: 1 to: 7447
pFN21AB8483 7447 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2043 2043
Size: 7447
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5257 278
Size: 53 83 186 936 3721 2468
Fragments arranged by size:
3721 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4790 1937
Size: 2853 4594
Acc65I G'GTAC_C
Cuts at: 4768 4768
Size: 7447
AclI AA'CG_TT
Cuts at: 1563 5574 5947 1563
Size: 4011 373 3063
Fragments arranged by size:
4011 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2592 3728 4757 5510 6894 7162 1632
Size: 960 1136 1029 753 1384 268 1917
Fragments arranged by size:
1917 1384 1136 1029 960 753 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7250
AflIII A'CryG_T
Cuts at: 1203 6346 7323 1203
Size: 5143 977 1327
Fragments arranged by size:
5143 1327 977
AgeI A'CCGG_T
Cuts at: 6278 6278
Size: 7447
AhdI GACnn_n'nnGTC
Cuts at: 4381 6176 4381
Size: 1795 5652
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7447
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3752 3816 1184
Size: 2568 64 4815
Fragments arranged by size:
4815 2568 64
AlwNI CAG_nnn'CTG
Cuts at: 3400 3616 4291 5190 6762 3400
Size: 216 675 899 1572 4085
Fragments arranged by size:
4085 1572 899 675 216
ApaI G_GGCC'C
Cuts at: 2028 4432 4531 2028
Size: 2404 99 4944
Fragments arranged by size:
4944 2404 99
ApaLI G'TGCA_C
Cuts at: 5504 6660 5504
Size: 1156 6291
ApoI r'AATT_y
Cuts at: 1493 2147 4756 4982 5134 7243 1493
Size: 654 2609 226 152 2109 1697
Fragments arranged by size:
2609 2109 1697 654 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6001 160
Size: 872 4969 1606
Fragments arranged by size:
4969 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
AvaI C'yCGr_G
Cuts at: 1870 1943 2471 2941 3140 4530 4772 1870
Size: 73 528 470 199 1390 242 4545
Fragments arranged by size:
4545 1390 528 470 242 199 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2675 2708 2675
Size: 33 7414
BamHI G'GATC_C
Cuts at: 4777 5144 4777
Size: 367 7080
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2500 3103 3969 4535 4703
Size: 325 217 627 713 603 866 566 168
Cuts at: 4703 4768 6223 618
Size: 65 1455 1842
Fragments arranged by size:
1842 1455 866 713 627 603 566 325
217 168 65
BanII G_rGCy'C
Cuts at: 1391 2028 2297 2708 2891 4432 4531 4596 4766
Size: 637 269 411 183 1541 99 65 170
Cuts at: 4766 1391
Size: 4072
Fragments arranged by size:
4072 1541 637 411 269 183 170 99
65
BbeI G_GCGC'C
Cuts at: 4707 4707
Size: 7447
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2928 3035 3824 928
Size: 971 1029 107 789 4551
Fragments arranged by size:
4551 1029 971 789 107
BbvCI CC'TCA_GC
Cuts at: 3532 3532
Size: 7447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4145 4179 5638 5672 1903
Size: 34 2208 34 1459 34 3678
Fragments arranged by size:
3678 2208 1459 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2676 5339 6549 2676
Size: 2663 1210 3574
Fragments arranged by size:
3574 2663 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7447
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4337 4355 6058
Size: 107 122 71 631 605 2665 18 1703
Cuts at: 6058 136
Size: 1525
Fragments arranged by size:
2665 1703 1525 631 605 122 107 71
18
BglII A'GATC_T
Cuts at: 7442 7442
Size: 7447
BlpI GC'TnA_GC
Cuts at: 2291 2541 3150 3168 4035 4296 4544 4864 2291
Size: 250 609 18 867 261 248 320 4874
Fragments arranged by size:
4874 867 609 320 261 250 248 18
Bme1580I G_kGCm'C
Cuts at: 1790 2028 3108 4425 4432 4531 5508 6664 1790
Size: 238 1080 1317 7 99 977 1156 2573
Fragments arranged by size:
2573 1317 1156 1080 977 238 99 7
BmgBI CAC'GTC
Cuts at: 1318 4378 1318
Size: 3060 4387
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2482 5178 6136
Size: 416 53 83 399 24 1065 2696 958
Cuts at: 6136 442
Size: 1753
Fragments arranged by size:
2696 1753 1065 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7447
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 7447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2586 2708 3101 3422 3638 3713 4169
Size: 821 526 122 393 321 216 75 456
Cuts at: 4169 4277 4371 4610 6107 1239
Size: 108 94 239 1497 2579
Fragments arranged by size:
2579 1497 821 526 456 393 321 239
216 122 108 94 75
Bpu10I CC'TnA_GC
Cuts at: 2774 3532 3935 2774
Size: 758 403 6286
Fragments arranged by size:
6286 758 403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 5572 6437 6735 2126
Size: 3446 865 298 2838
Fragments arranged by size:
3446 2838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2794 6110 882
Size: 617 1295 3316 2219
Fragments arranged by size:
3316 2219 1295 617
BsaAI yAC'GTr
Cuts at: 493 1204 3929 5238 493
Size: 711 2725 1309 2702
Fragments arranged by size:
2725 2702 1309 711
BsaBI GATnn'nnATC
Cuts at: 2519 7441 2519
Size: 4922 2525
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2302 4704 5254 5636
Size: 53 83 186 936 769 2402 550 382
Cuts at: 5636 6271 275
Size: 635 1451
Fragments arranged by size:
2402 1451 936 769 635 550 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3480 5880 6278 6552 6699 7110 3480
Size: 2400 398 274 147 411 3817
Fragments arranged by size:
3817 2400 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3462 3492 1178
Size: 30 165 30 2059 30 5133
Fragments arranged by size:
5133 2059 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 3158 3404 3425 3653 3686 3710 3785
Size: 949 1023 246 21 228 33 24 75
Cuts at: 3785 3851 3866 3905 3935 3968 4016 4274 4328
Size: 66 15 39 30 33 48 258 54
Cuts at: 4328 1186
Size: 4305
Fragments arranged by size:
4305 1023 949 258 246 228 75 66
54 48 39 33 33 30 24 21
15
BseYI C'CCAG_C
Cuts at: 2556 3454 4715 6650 7146 2556
Size: 898 1261 1935 496 2857
Fragments arranged by size:
2857 1935 1261 898 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2406 3476 3521 4400 1163
Size: 573 670 1070 45 879 4210
Fragments arranged by size:
4210 1070 879 670 573 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2928 4029 4897 5658 6686 1540
Size: 275 186 927 1101 868 761 1028 2301
Fragments arranged by size:
2301 1101 1028 927 868 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2297 2708 4596 5508 5593 6664 2297
Size: 411 1888 912 85 1071 3080
Fragments arranged by size:
3080 1888 1071 912 411 85
BsmI GAATG_Cn'
Cuts at: 2542 4958 5051 2542
Size: 2416 93 4938
Fragments arranged by size:
4938 2416 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2203 2203
Size: 7447
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2297 2708 2891 3108 4425 4432
Size: 399 238 269 411 183 217 1317 7
Cuts at: 4432 4531 4596 4766 5508 5593 6664 1391
Size: 99 65 170 742 85 1071 2174
Fragments arranged by size:
2174 1317 1071 742 411 399 269 238
217 183 170 99 85 65 7
BspEI T'CCGG_A
Cuts at: 3480 7110 3480
Size: 3630 3817
BspHI T'CATG_A
Cuts at: 3519 3549 5336 7066 3519
Size: 30 1787 1730 3900
Fragments arranged by size:
3900 1787 1730 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2043 2776 4802 844
Size: 1199 733 2026 3489
Fragments arranged by size:
3489 2026 1199 733
BsrBI CCG'CTC
Cuts at: 1394 1823 2842 3607 3751 3841 3964 4012 4066
Size: 429 1019 765 144 90 123 48 54
Cuts at: 4066 4081 4096 4111 4126 4180 4210 4300 4543
Size: 15 15 15 15 54 30 90 243
Cuts at: 4543 5334 1394
Size: 791 3507
Fragments arranged by size:
3507 1019 791 765 429 243 144 123
90 90 54 54 48 30 15 15
15 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 5942 6116 65
Size: 1183 4694 174 1396
Fragments arranged by size:
4694 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6091 6278 1700
Size: 110 4281 187 2869
Fragments arranged by size:
4281 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7447
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7447
BssSI C'ACGA_G
Cuts at: 3202 5507 6519 3202
Size: 2305 1012 4130
Fragments arranged by size:
4130 2305 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2420 3499 794
Size: 1626 1079 4742
Fragments arranged by size:
4742 1626 1079
BstBI TT'CG_AA
Cuts at: 6251 6251
Size: 7447
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7447
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2686 3116 3317 3466 3886 4723 2416
Size: 270 430 201 149 420 837 5140
Fragments arranged by size:
5140 837 430 420 270 201 149
BstYI r'GATC_y
Cuts at: 1972 2242 4465 4777 5144 5529 5546 6998 7084
Size: 270 2223 312 367 385 17 1452 86
Cuts at: 7084 7096 7442 1972
Size: 12 346 1977
Fragments arranged by size:
2223 1977 1452 385 367 346 312 270
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2400 2409 2801 2831 2883 513
Size: 552 939 396 9 392 30 52 5077
Fragments arranged by size:
5077 939 552 396 392 52 30 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6708
Fragments arranged by size:
6708 683 56
BtsI GCAGTG_nn'
Cuts at: 4963 5757 5777 4963
Size: 794 20 6633
Fragments arranged by size:
6633 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7447
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4602 4637 529
Size: 35 4038 35 3339
Fragments arranged by size:
4038 3339 35 35
DraI TTT'AAA
Cuts at: 4751 4751
Size: 7447
DraIII CAC_nnn'GTG
Cuts at: 2490 3499 2490
Size: 1009 6438
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2758 6454 809
Size: 507 1442 3696 1802
Fragments arranged by size:
3696 1802 1442 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2979 3055 3876
Size: 54 1425 50 275 18 1149 76 821
Cuts at: 3876 4026 4894 5783 7412 8
Size: 150 868 889 1629 43
Fragments arranged by size:
1629 1425 1149 889 868 821 275 150
76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4026 4894 1537
Size: 275 2214 868 4090
Fragments arranged by size:
4090 2214 868 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 3033 3912 3954 3993 4272 4634 5377
Size: 848 832 879 42 39 279 362 743
Cuts at: 5377 1353
Size: 3423
Fragments arranged by size:
3423 879 848 832 743 362 279 42
39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3646 6034 6408 6554 387
Size: 1064 138 2057 2388 374 146 1280
Fragments arranged by size:
2388 2057 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2295 2706 4594 2295
Size: 411 1888 5148
Fragments arranged by size:
5148 1888 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2586 2592 2708 3101 3422 3638
Size: 393 428 526 6 116 393 321 216
Cuts at: 3638 3713 3728 4169 4277 4371 4610 4757 5510
Size: 75 15 441 108 94 239 147 753
Cuts at: 5510 6107 6894 7162 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 526 441 428 393
393 321 268 239 216 147 116 108
94 75 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3728 3728
Size: 7447
EcoO109I rG'GnC_Cy
Cuts at: 2024 2728 3178 4219 4357 4428 4429 4711 2024
Size: 704 450 1041 138 71 1 282 4760
Fragments arranged by size:
4760 1041 704 450 282 138 71 1
EcoRI G'AATT_C
Cuts at: 2147 4756 5134 2147
Size: 2609 378 4460
Fragments arranged by size:
4460 2609 378
EcoRV GAT'ATC
Cuts at: 4630 4630
Size: 7447
FspI TGC'GCA
Cuts at: 2784 3584 3656 5953 2784
Size: 800 72 2297 4278
Fragments arranged by size:
4278 2297 800 72
HaeII r_GCGC'y
Cuts at: 1678 2533 3616 4707 6594 1678
Size: 855 1083 1091 1887 2531
Fragments arranged by size:
2531 1887 1091 1083 855
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2264 2639 2671 2858
Size: 32 135 32 660 32 375 32 187
Cuts at: 2858 2890 2891 2923 4367 4399 6088 6120 6162
Size: 32 1 32 1444 32 1689 32 42
Cuts at: 6162 6194 1373
Size: 32 2626
Fragments arranged by size:
2626 1689 1444 660 375 187 135 42
32 32 32 32 32 32 32 32
32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4791 5037 669
Size: 988 281 2853 246 3079
Fragments arranged by size:
3079 2853 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7447
HpaI GTT'AAC
Cuts at: 5037 5037
Size: 7447
KasI G'GCGC_C
Cuts at: 4703 4703
Size: 7447
KpnI G_GTAC'C
Cuts at: 4772 4772
Size: 7447
MfeI C'AATT_G
Cuts at: 5046 5046
Size: 7447
MluI A'CGCG_T
Cuts at: 7323 7323
Size: 7447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2645 6561 6745 1305
Size: 1340 3916 184 2007
Fragments arranged by size:
3916 2007 1340 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2981 10
Size: 54 1425 343 1149 4476
Fragments arranged by size:
4476 1425 1149 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 5405 5764 5923 518
Size: 648 1533 2706 359 159 2042
Fragments arranged by size:
2706 2042 1533 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2290 2396 2402 2497 3047 3167 3652 4054
Size: 684 106 6 95 550 120 485 402
Cuts at: 4054 4147 4243 4306 4863 5540 6688 6933 1606
Size: 93 96 63 557 677 1148 245 2120
Fragments arranged by size:
2120 1148 684 677 557 550 485 402
245 120 106 96 95 93 63 6
NarI GG'CG_CC
Cuts at: 4704 4704
Size: 7447
NcoI C'CATG_G
Cuts at: 513 1065 2004 2409 2831 513
Size: 552 939 405 422 5129
Fragments arranged by size:
5129 939 552 422 405
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7447
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7447
NotI GC'GGCC_GC
Cuts at: 4894 4894
Size: 7447
NspI r_CATG'y
Cuts at: 1132 1229 2137 2552 4805 6350 1132
Size: 97 908 415 2253 1545 2229
Fragments arranged by size:
2253 2229 1545 908 415 97
PasI CC'CwG_GG
Cuts at: 1348 2021 2629 1348
Size: 673 608 6166
Fragments arranged by size:
6166 673 608
PciI A'CATG_T
Cuts at: 6346 6346
Size: 7447
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7447
PmeI GTTT'AAAC
Cuts at: 4751 4751
Size: 7447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2120 2152 5516 5548 7029 7061 2120
Size: 32 3364 32 1481 32 2506
Fragments arranged by size:
3364 2506 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2728 3178 4711 2728
Size: 450 1533 5464
Fragments arranged by size:
5464 1533 450
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7447
PsiI TTA'TAA
Cuts at: 5017 5017
Size: 7447
PspOMI G'GGCC_C
Cuts at: 2024 4428 4527 2024
Size: 2404 99 4944
Fragments arranged by size:
4944 2404 99
PstI C_TGCA'G
Cuts at: 830 2395 3259 3286 3420 3741 3948 3987 4206
Size: 1565 864 27 134 321 207 39 219
Cuts at: 4206 4233 4248 4311 4701 4799 830
Size: 27 15 63 390 98 3478
Fragments arranged by size:
3478 1565 864 390 321 219 207 134
98 63 39 27 27 15
PvuI CG_AT'CG
Cuts at: 2001 2928 2001
Size: 927 6520
PvuII CAG'CTG
Cuts at: 2290 2396 2497 3047 3167 3652 4147 4243 4306
Size: 106 101 550 120 485 495 96 63
Cuts at: 4306 2290
Size: 5431
Fragments arranged by size:
5431 550 495 485 120 106 101 96
63
SacI G_AGCT'C
Cuts at: 2297 2708 4596 2297
Size: 411 1888 5148
Fragments arranged by size:
5148 1888 411
SacII CC_GC'GG
Cuts at: 2403 2403
Size: 7447
SalI G'TCGA_C
Cuts at: 1936 4789 1936
Size: 2853 4594
SanDI GG'GwC_CC
Cuts at: 4711 4711
Size: 7447
SapI GCTCTTCn'nnn_
Cuts at: 3033 3954 4272 4634 3033
Size: 921 318 362 5846
Fragments arranged by size:
5846 921 362 318
SbfI CC_TGCA'GG
Cuts at: 4701 4799 4701
Size: 98 7349
ScaI AGT'ACT
Cuts at: 2969 4568 5695 7407 2969
Size: 1599 1127 1712 3009
Fragments arranged by size:
3009 1712 1599 1127
SfcI C'TryA_G
Cuts at: 826 1045 2337 2391 3255 3282 3416 3737 3944
Size: 219 1292 54 864 27 134 321 207
Cuts at: 3944 3983 4202 4229 4244 4307 4697 4729 4795
Size: 39 219 27 15 63 390 32 66
Cuts at: 4795 5930 6611 6802 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1292 1135 864 681 390 321 219
219 207 191 134 66 63 54 39
32 27 27 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 4355 4355
Size: 7447
SfoI GGC'GCC
Cuts at: 4705 4705
Size: 7447
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7447
SmaI CCC'GGG
Cuts at: 1872 4774 1872
Size: 2902 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 5551 6452 6714 820
Size: 197 926 162 3446 901 262 1553
Fragments arranged by size:
3446 1553 926 901 262 197 162
SnaBI TAC'GTA
Cuts at: 493 5238 493
Size: 4745 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7447
SphI G_CATG'C
Cuts at: 1132 4805 1132
Size: 3673 3774
SspI AAT'ATT
Cuts at: 5 52 5371 5
Size: 47 5319 2081
Fragments arranged by size:
5319 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7447
StyI C'CwwG_G
Cuts at: 513 1065 2004 2409 2733 2831 3594 3831 4332
Size: 552 939 405 324 98 763 237 501
Cuts at: 4332 4886 513
Size: 554 3074
Fragments arranged by size:
3074 939 763 554 552 501 405 324
237 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2449 2501 2570 3256 4590 5474 5491
Size: 1255 869 52 69 686 1334 884 17
Cuts at: 5491 5644 5829 6274 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1334 1255 884 869 686 445 185
153 69 52 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2967 4566 5693
Size: 274 80 33 51 1443 990 1599 1127
Cuts at: 5693 7405 96
Size: 1712 138
Fragments arranged by size:
1712 1599 1443 1127 990 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 7407 7439 2084
Size: 32 5291 32 2092
Fragments arranged by size:
5291 2092 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7447
XbaI T'CTAG_A
Cuts at: 4783 4783
Size: 7447
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 2829 4612 2035
Size: 794 1783 4870
Fragments arranged by size:
4870 1783 794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7447
XmaI C'CCGG_G
Cuts at: 1870 4772 1870
Size: 2902 4545
XmnI GAAnn'nnTTC
Cuts at: 5576 5576
Size: 7447
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5255 276
Size: 53 83 186 936 3721 2468
Fragments arranged by size:
3721 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SexAI SrfI SwaI