Restriction map for
pFN21AB8478
(Circular) (Six-base) MAPSORT of: pFN21AB8478.seq Check: 4833 from: 1 to: 7246
pFN21AB8478 7246 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3050 3342 3382 4462 3050
Size: 292 40 1080 5834
Fragments arranged by size:
5834 1080 292 40
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2919 3658 5056 278
Size: 53 83 186 936 1383 739 1398 2468
Fragments arranged by size:
2468 1398 1383 936 739 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4589 1937
Size: 2652 4594
Acc65I G'GTAC_C
Cuts at: 3774 4567 3774
Size: 793 6453
AclI AA'CG_TT
Cuts at: 1563 5373 5746 1563
Size: 3810 373 3063
Fragments arranged by size:
3810 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2813 5309 6693 6961 1632
Size: 1181 2496 1384 268 1917
Fragments arranged by size:
2496 1917 1384 1181 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7049
AflIII A'CryG_T
Cuts at: 1203 6145 7122 1203
Size: 4942 977 1327
Fragments arranged by size:
4942 1327 977
AgeI A'CCGG_T
Cuts at: 6077 6077
Size: 7246
AhdI GACnn_n'nnGTC
Cuts at: 5975 5975
Size: 7246
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3530 3971 1184
Size: 2346 441 4459
Fragments arranged by size:
4459 2346 441
AlwNI CAG_nnn'CTG
Cuts at: 2353 4989 6561 2353
Size: 2636 1572 3038
Fragments arranged by size:
3038 2636 1572
ApaI G_GGCC'C
Cuts at: 2604 4478 2604
Size: 1874 5372
ApaLI G'TGCA_C
Cuts at: 5303 6459 5303
Size: 1156 6090
ApoI r'AATT_y
Cuts at: 1493 4555 4781 4933 7042 1493
Size: 3062 226 152 2109 1697
Fragments arranged by size:
3062 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5800 160
Size: 872 4768 1606
Fragments arranged by size:
4768 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
AvaI C'yCGr_G
Cuts at: 1870 1943 2220 2307 2817 3047 3863 4571 1870
Size: 73 277 87 510 230 816 708 4545
Fragments arranged by size:
4545 816 708 510 277 230 87 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4138 4171 4138
Size: 33 7213
BamHI G'GATC_C
Cuts at: 4576 4943 4576
Size: 367 6879
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2707 2855 2962 3774 3947
Size: 325 217 627 920 148 107 812 173
Cuts at: 3947 4256 4265 4511 4528 4567 6022 618
Size: 309 9 246 17 39 1455 1842
Fragments arranged by size:
1842 1455 920 812 627 325 309 246
217 173 148 107 39 17 9
BanII G_rGCy'C
Cuts at: 1391 2013 2039 2166 2565 2604 2617 3054 3864
Size: 622 26 127 399 39 13 437 810
Cuts at: 3864 3873 4187 4206 4478 4565 1391
Size: 9 314 19 272 87 4072
Fragments arranged by size:
4072 810 622 437 399 314 272 127
87 39 26 19 13 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3544 928
Size: 971 1645 4630
Fragments arranged by size:
4630 1645 971
BbvCI CC'TCA_GC
Cuts at: 2606 2606
Size: 7246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3472 3506 5437 5471 1903
Size: 34 1535 34 1931 34 3678
Fragments arranged by size:
3678 1931 1535 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4166 5138 6348 4166
Size: 972 1210 5064
Fragments arranged by size:
5064 1210 972
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7246
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2170 2280 2516
Size: 107 122 71 631 605 498 110 236
Cuts at: 2516 2543 2870 3877 5857 136
Size: 27 327 1007 1980 1525
Fragments arranged by size:
1980 1525 1007 631 605 498 327 236
122 110 107 71 27
BglII A'GATC_T
Cuts at: 7241 7241
Size: 7246
BlpI GC'TnA_GC
Cuts at: 4663 4663
Size: 7246
Bme1580I G_kGCm'C
Cuts at: 1790 2604 2712 2858 4478 4495 4516 5307 6463
Size: 814 108 146 1620 17 21 791 1156
Cuts at: 6463 1790
Size: 2573
Fragments arranged by size:
2573 1620 1156 814 791 146 108 21
17
BmgBI CAC'GTC
Cuts at: 1318 4193 1318
Size: 2875 4371
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2160 2665 3337
Size: 416 53 83 399 24 743 505 672
Cuts at: 3337 3702 4977 5935 442
Size: 365 1275 958 1753
Fragments arranged by size:
1753 1275 958 743 672 505 416 399
365 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 2576 2941 3161 3680 3878 4360 5906
Size: 1142 195 365 220 519 198 482 1546
Cuts at: 5906 1239
Size: 2579
Fragments arranged by size:
2579 1546 1142 519 482 365 220 198
195
Bpu10I CC'TnA_GC
Cuts at: 2606 2606
Size: 7246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2525 2714 3652 4088 5371 6236 6534 2015
Size: 510 189 938 436 1283 865 298 2727
Fragments arranged by size:
2727 1283 938 865 510 436 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2112 3224 5909 882
Size: 617 613 1112 2685 2219
Fragments arranged by size:
2685 2219 1112 617 613
BsaAI yAC'GTr
Cuts at: 493 1204 3415 5037 493
Size: 711 2211 1622 2702
Fragments arranged by size:
2702 2211 1622 711
BsaBI GATnn'nnATC
Cuts at: 7240 7240
Size: 7246
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2916 3655 3914 4109
Size: 53 83 186 936 1383 739 259 195
Cuts at: 4109 5053 5435 6070 275
Size: 944 382 635 1451
Fragments arranged by size:
1451 1383 944 936 739 635 382 259
195 186 83 53
BsaWI w'CCGG_w
Cuts at: 3537 5679 6077 6351 6498 6909 3537
Size: 2142 398 274 147 411 3874
Fragments arranged by size:
3874 2142 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 3098 3128 4203
Size: 30 165 30 798 30 867 30 1075
Cuts at: 4203 4233 1178
Size: 30 4191
Fragments arranged by size:
4191 1075 867 798 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2103 2378 2573 3281 3284 3556 3757 3881
Size: 917 275 195 708 3 272 201 124
Cuts at: 3881 4211 4214 1186
Size: 330 3 4218
Fragments arranged by size:
4218 917 708 330 275 272 201 195
124 3 3
BseYI C'CCAG_C
Cuts at: 3078 3693 3823 3967 4355 6449 6945 3078
Size: 615 130 144 388 2094 496 3379
Fragments arranged by size:
3379 2094 615 496 388 144 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2675 2684 3536 4116 4377 1163
Size: 573 939 9 852 580 261 4032
Fragments arranged by size:
4032 939 852 580 573 261 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3410 4068 4696 5457 6485 1540
Size: 275 186 1409 658 628 761 1028 2301
Fragments arranged by size:
2301 1409 1028 761 658 628 275 186
BsiHKAI G_wGCw'C
Cuts at: 2617 2784 3333 4408 5307 5392 6463 2617
Size: 167 549 1075 899 85 1071 3400
Fragments arranged by size:
3400 1075 1071 899 549 167 85
BsmI GAATG_Cn'
Cuts at: 4757 4850 4757
Size: 93 7153
BsmBI CGTCTCn'nnnn_
Cuts at: 2235 2235
Size: 7246
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2039 2166 2565 2604 2617 2712
Size: 399 223 26 127 399 39 13 95
Cuts at: 2712 2784 2858 3054 3333 3864 3873 4187 4206
Size: 72 74 196 279 531 9 314 19
Cuts at: 4206 4408 4478 4495 4516 4565 5307 5392 6463
Size: 202 70 17 21 49 742 85 1071
Cuts at: 6463 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 531 399 399 314 279
223 202 196 127 95 85 74 72
70 49 39 26 21 19 17 13
9
BspEI T'CCGG_A
Cuts at: 6909 6909
Size: 7246
BspHI T'CATG_A
Cuts at: 5135 6865 5135
Size: 1730 5516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2697 3050 3342 3382 3993 4462 4601 844
Size: 1853 353 292 40 611 469 139 3489
Fragments arranged by size:
3489 1853 611 469 353 292 139 40
BsrBI CCG'CTC
Cuts at: 1394 1823 3284 3682 4087 5133 1394
Size: 429 1461 398 405 1046 3507
Fragments arranged by size:
3507 1461 1046 429 405 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 3679 5741 5915 65
Size: 1183 1284 1147 2062 174 1396
Fragments arranged by size:
2062 1396 1284 1183 1147 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2094 2594 5890 6077 1700
Size: 110 284 500 3296 187 2869
Fragments arranged by size:
3296 2869 500 284 187 110
BsrGI T'GTAC_A
Cuts at: 96 2397 96
Size: 2301 4945
BssHII G'CGCG_C
Cuts at: 1925 3287 3458 1925
Size: 1362 171 5713
Fragments arranged by size:
5713 1362 171
BssSI C'ACGA_G
Cuts at: 5306 6318 5306
Size: 1012 6234
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2689 2982 794
Size: 1895 293 5058
Fragments arranged by size:
5058 1895 293
BstBI TT'CG_AA
Cuts at: 6050 6050
Size: 7246
BstEII G'GTnAC_C
Cuts at: 1184 2198 3836 1184
Size: 1014 1638 4594
Fragments arranged by size:
4594 1638 1014
BstXI CCAn_nnnn'nTGG
Cuts at: 2270 3607 4507 2270
Size: 1337 900 5009
Fragments arranged by size:
5009 1337 900
BstYI r'GATC_y
Cuts at: 1972 3210 3806 4576 4943 5328 5345 6797 6883
Size: 1238 596 770 367 385 17 1452 86
Cuts at: 6883 6895 7241 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1238 770 596 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2100 2189 2877 3802 2100
Size: 89 688 925 5544
Fragments arranged by size:
5544 925 688 89
BtgI C'CryG_G
Cuts at: 513 1065 2004 2674 2850 2971 3167 3427 3845
Size: 552 939 670 176 121 196 260 418
Cuts at: 3845 4207 4361 4523 513
Size: 362 154 162 3236
Fragments arranged by size:
3236 939 670 552 418 362 260 196
176 162 154 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6507
Fragments arranged by size:
6507 683 56
BtsI GCAGTG_nn'
Cuts at: 2065 2692 2917 3023 4762 5556 5576 2065
Size: 627 225 106 1739 794 20 3735
Fragments arranged by size:
3735 1739 794 627 225 106 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7246
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7211
DraI TTT'AAA
Cuts at: 4550 4550
Size: 7246
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6253 809
Size: 507 4937 1802
Fragments arranged by size:
4937 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2272 2337 2619
Size: 54 1425 50 275 18 442 65 282
Cuts at: 2619 3069 3976 4693 5582 7211 8
Size: 450 907 717 889 1629 43
Fragments arranged by size:
1629 1425 907 889 717 450 442 282
275 65 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4693 1537
Size: 275 2881 4090
Fragments arranged by size:
4090 2881 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2030 2510 3227 5176 1353
Size: 659 18 480 717 1949 3423
Fragments arranged by size:
3423 1949 717 659 480 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3323 5833 6207 6353 387
Size: 1064 138 1734 2510 374 146 1280
Fragments arranged by size:
2510 1734 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2615 2615
Size: 7246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2576 2813 2941 3161 3680 3878
Size: 393 749 195 237 128 220 519 198
Cuts at: 3878 4360 5309 5906 6693 6961 1239
Size: 482 949 597 787 268 1524
Fragments arranged by size:
1524 949 787 749 597 519 482 393
268 237 220 198 195 128
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 7246
EcoO109I rG'GnC_Cy
Cuts at: 2449 2601 2701 3088 3151 3704 3750 4474 4475
Size: 152 100 387 63 553 46 724 1
Cuts at: 4475 4519 4541 2449
Size: 44 22 5154
Fragments arranged by size:
5154 724 553 387 152 100 63 46
44 22 1
EcoRI G'AATT_C
Cuts at: 4555 4933 4555
Size: 378 6868
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 7246
FseI GG_CCGG'CC
Cuts at: 2098 2098
Size: 7246
FspI TGC'GCA
Cuts at: 5752 5752
Size: 7246
HaeII r_GCGC'y
Cuts at: 1678 3140 3938 6393 1678
Size: 1462 798 2455 2531
Fragments arranged by size:
2531 2455 1462 798
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2233 2717 2749 3098
Size: 32 135 32 629 32 484 32 349
Cuts at: 3098 3130 3202 3234 3284 3316 3779 3811 4203
Size: 32 72 32 50 32 463 32 392
Cuts at: 4203 4235 5887 5919 5961 5993 1373
Size: 32 1652 32 42 32 2626
Fragments arranged by size:
2626 1652 629 484 463 392 349 135
72 50 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2653 3013 4590 4836 669
Size: 988 281 715 360 1577 246 3079
Fragments arranged by size:
3079 1577 988 715 360 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7246
HpaI GTT'AAC
Cuts at: 3013 4836 3013
Size: 1823 5423
KpnI G_GTAC'C
Cuts at: 3778 4571 3778
Size: 793 6453
MfeI C'AATT_G
Cuts at: 4845 4845
Size: 7246
MluI A'CGCG_T
Cuts at: 7122 7122
Size: 7246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2319 2760 3111 3588 6360 6544 1305
Size: 1014 441 351 477 2772 184 2007
Fragments arranged by size:
2772 2007 1014 477 441 351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2274 2339 10
Size: 54 1425 343 442 65 4917
Fragments arranged by size:
4917 1425 442 343 65 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 2840 3512 5204 5563 5722 518
Size: 648 1302 372 672 1692 359 159 2042
Fragments arranged by size:
2042 1692 1302 672 648 372 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 2120 2409 2852 3169 3298 3316 3429
Size: 461 53 289 443 317 129 18 113
Cuts at: 3429 3847 3931 4171 4209 4355 4662 5339 6487
Size: 418 84 240 38 146 307 677 1148
Cuts at: 6487 6732 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 461 443 418 317 307
289 245 240 146 129 113 84 53
38 18
NaeI GCC'GGC
Cuts at: 2096 2096
Size: 7246
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5755
Fragments arranged by size:
5755 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7246
NgoMIV G'CCGG_C
Cuts at: 2094 2094
Size: 7246
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7246
NotI GC'GGCC_GC
Cuts at: 4693 4693
Size: 7246
NspI r_CATG'y
Cuts at: 1132 1229 3487 3693 4604 6149 1132
Size: 97 2258 206 911 1545 2229
Fragments arranged by size:
2258 2229 1545 911 206 97
PasI CC'CwG_GG
Cuts at: 1348 4285 1348
Size: 2937 4309
PciI A'CATG_T
Cuts at: 6145 6145
Size: 7246
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 2972 1108
Size: 1539 325 5382
Fragments arranged by size:
5382 1539 325
PfoI T'CCnGG_A
Cuts at: 2346 4422 2346
Size: 2076 5170
PmeI GTTT'AAAC
Cuts at: 4550 4550
Size: 7246
PmlI CAC'GTG
Cuts at: 3415 3415
Size: 7246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5315 5347 6828 6860 5315
Size: 32 1481 32 5701
Fragments arranged by size:
5701 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2449 3088 3151 3704 3750 2449
Size: 639 63 553 46 5945
Fragments arranged by size:
5945 639 553 63 46
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7246
PsiI TTA'TAA
Cuts at: 4816 4816
Size: 7246
PspOMI G'GGCC_C
Cuts at: 2600 4474 2600
Size: 1874 5372
PstI C_TGCA'G
Cuts at: 830 2757 3126 3390 3619 3990 4490 4598 830
Size: 1927 369 264 229 371 500 108 3478
Fragments arranged by size:
3478 1927 500 371 369 264 229 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7246
PvuII CAG'CTG
Cuts at: 2409 3316 4355 2409
Size: 907 1039 5300
Fragments arranged by size:
5300 1039 907
SacI G_AGCT'C
Cuts at: 2617 2617
Size: 7246
SacII CC_GC'GG
Cuts at: 2853 3170 3430 3848 4210 2853
Size: 317 260 418 362 5889
Fragments arranged by size:
5889 418 362 317 260
SalI G'TCGA_C
Cuts at: 1936 4588 1936
Size: 2652 4594
SanDI GG'GwC_CC
Cuts at: 3704 3704
Size: 7246
SapI GCTCTTCn'nnn_
Cuts at: 2012 2510 2012
Size: 498 6748
SbfI CC_TGCA'GG
Cuts at: 4598 4598
Size: 7246
ScaI AGT'ACT
Cuts at: 5494 7206 5494
Size: 1712 5534
SfcI C'TryA_G
Cuts at: 826 1045 2753 3122 3386 3615 3986 4486 4594
Size: 219 1708 369 264 229 371 500 108
Cuts at: 4594 5729 6410 6601 826
Size: 1135 681 191 1471
Fragments arranged by size:
1708 1471 1135 681 500 371 369 264
229 219 191 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7246
SmaI CCC'GGG
Cuts at: 1872 2222 4573 1872
Size: 350 2351 4545
Fragments arranged by size:
4545 2351 350
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 2540 2729 3667 4103 5350
Size: 197 926 87 510 189 938 436 1247
Cuts at: 5350 6251 6513 820
Size: 901 262 1553
Fragments arranged by size:
1553 1247 938 926 901 510 436 262
197 189 87
SnaBI TAC'GTA
Cuts at: 493 5037 493
Size: 4544 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7246
SphI G_CATG'C
Cuts at: 1132 3487 3693 4604 1132
Size: 2355 206 911 3774
Fragments arranged by size:
3774 2355 911 206
SspI AAT'ATT
Cuts at: 5 52 5170 5
Size: 47 5118 2081
Fragments arranged by size:
5118 2081 47
StuI AGG'CCT
Cuts at: 1517 2891 3521 3880 1517
Size: 1374 630 359 4883
Fragments arranged by size:
4883 1374 630 359
StyI C'CwwG_G
Cuts at: 513 1065 2004 3240 3973 4685 513
Size: 552 939 1236 733 712 3074
Fragments arranged by size:
3074 1236 939 733 712 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 4082 4499 4516 5273 5290 5443
Size: 1255 678 1824 417 17 757 17 153
Cuts at: 5443 5628 6073 325
Size: 185 445 1498
Fragments arranged by size:
1824 1498 1255 757 678 445 417 185
153 17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 5492 7204
Size: 274 80 33 51 1443 420 3095 1712
Cuts at: 7204 96
Size: 138
Fragments arranged by size:
3095 1712 1443 420 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2044 2076 3015 3047 7206 7238 2044
Size: 32 939 32 4159 32 2052
Fragments arranged by size:
4159 2052 939 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7246
XbaI T'CTAG_A
Cuts at: 4582 4582
Size: 7246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2572 3630 3775 2572
Size: 1058 145 6043
Fragments arranged by size:
6043 1058 145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7246
XmaI C'CCGG_G
Cuts at: 1870 2220 4571 1870
Size: 350 2351 4545
Fragments arranged by size:
4545 2351 350
XmnI GAAnn'nnTTC
Cuts at: 5375 5375
Size: 7246
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2917 3656 5054 276
Size: 53 83 186 936 1383 739 1398 2468
Fragments arranged by size:
2468 1398 1383 936 739 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SexAI SfiI SfoI SrfI
SwaI