Restriction map for
pFN21AB8476
(Circular) (Six-base) MAPSORT of: pFN21AB8476.seq Check: 5130 from: 1 to: 7243
pFN21AB8476 7243 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2106 5053 278
Size: 53 83 186 936 570 2947 2468
Fragments arranged by size:
2947 2468 936 570 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4586 1937
Size: 2649 4594
Acc65I G'GTAC_C
Cuts at: 4564 4564
Size: 7243
AclI AA'CG_TT
Cuts at: 1563 5370 5743 1563
Size: 3807 373 3063
Fragments arranged by size:
3807 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2630 2937 2991 3231 3612 3935 5306 6690
Size: 998 307 54 240 381 323 1371 1384
Cuts at: 6690 6958 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1371 998 381 323 307 268
240 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7046
AflIII A'CryG_T
Cuts at: 1203 6142 7119 1203
Size: 4939 977 1327
Fragments arranged by size:
4939 1327 977
AgeI A'CCGG_T
Cuts at: 6074 6074
Size: 7243
AhdI GACnn_n'nnGTC
Cuts at: 5972 5972
Size: 7243
AleI CACnn'nnGTG
Cuts at: 1166 3661 1166
Size: 2495 4748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7243
AlwNI CAG_nnn'CTG
Cuts at: 4986 6558 4986
Size: 1572 5671
ApaLI G'TGCA_C
Cuts at: 5300 6456 5300
Size: 1156 6087
ApoI r'AATT_y
Cuts at: 1493 3016 3097 3285 3830 3974 4238 4552 4778
Size: 1523 81 188 545 144 264 314 226
Cuts at: 4778 4930 7039 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1523 545 314 264 226 188
152 144 81
AseI AT'TA_AT
Cuts at: 160 1032 3531 5797 160
Size: 872 2499 2266 1606
Fragments arranged by size:
2499 2266 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7243
AvaI C'yCGr_G
Cuts at: 1870 1943 4568 1870
Size: 73 2625 4545
Fragments arranged by size:
4545 2625 73
BamHI G'GATC_C
Cuts at: 4573 4940 4573
Size: 367 6876
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2534 4564 6019 618
Size: 325 217 627 303 444 2030 1455 1842
Fragments arranged by size:
2030 1842 1455 627 444 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2683 2860 2943 3280 4562 1391
Size: 1292 177 83 337 1282 4072
Fragments arranged by size:
4072 1292 1282 337 177 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3219 3301 928
Size: 971 1320 82 4870
Fragments arranged by size:
4870 1320 971 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5434 5468 1903
Size: 34 3497 34 3678
Fragments arranged by size:
3678 3497 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3543 5135 6345 3543
Size: 1592 1210 4441
Fragments arranged by size:
4441 1592 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7243
BfrBI ATG'CAT
Cuts at: 2416 2416
Size: 7243
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5854 136
Size: 107 122 71 631 605 337 3845 1525
Fragments arranged by size:
3845 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2969 7238 2969
Size: 4269 2974
BlpI GC'TnA_GC
Cuts at: 4660 4660
Size: 7243
Bme1580I G_kGCm'C
Cuts at: 1790 5304 6460 1790
Size: 3514 1156 2573
Fragments arranged by size:
3514 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7243
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4974 5932 442
Size: 416 53 83 399 24 3557 958 1753
Fragments arranged by size:
3557 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 5903 1239
Size: 951 3713 2579
Fragments arranged by size:
3713 2579 951
Bpu10I CC'TnA_GC
Cuts at: 3186 3186
Size: 7243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5368 6233 6531 5368
Size: 865 298 6080
Fragments arranged by size:
6080 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2852 5906 882
Size: 617 1353 3054 2219
Fragments arranged by size:
3054 2219 1353 617
BsaAI yAC'GTr
Cuts at: 493 1204 5034 493
Size: 711 3830 2702
Fragments arranged by size:
3830 2702 711
BsaBI GATnn'nnATC
Cuts at: 3085 7237 3085
Size: 4152 3091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 5050 5432 6067
Size: 53 83 186 936 570 2947 382 635
Cuts at: 6067 275
Size: 1451
Fragments arranged by size:
2947 1451 936 635 570 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3223 5676 6074 6348 6495 6906 3223
Size: 2453 398 274 147 411 3560
Fragments arranged by size:
3560 2453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7018
Fragments arranged by size:
7018 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3033 1186
Size: 1847 5396
BseYI C'CCAG_C
Cuts at: 6446 6942 6446
Size: 496 6747
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4185 4482 1163
Size: 573 2449 297 3924
Fragments arranged by size:
3924 2449 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4693 5454 6482 1540
Size: 275 186 2692 761 1028 2301
Fragments arranged by size:
2692 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3280 5304 5389 6460 3280
Size: 2024 85 1071 4063
Fragments arranged by size:
4063 2024 1071 85
BsmI GAATG_Cn'
Cuts at: 2989 4754 4847 2989
Size: 1765 93 5385
Fragments arranged by size:
5385 1765 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2683 2860 2943 3280 4562 5304 5389
Size: 399 893 177 83 337 1282 742 85
Cuts at: 5389 6460 1391
Size: 1071 2174
Fragments arranged by size:
2174 1282 1071 893 742 399 337 177
85 83
BspEI T'CCGG_A
Cuts at: 3223 6906 3223
Size: 3683 3560
BspHI T'CATG_A
Cuts at: 5132 6862 5132
Size: 1730 5513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4598 844
Size: 3754 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5130 1394
Size: 429 3307 3507
Fragments arranged by size:
3507 3307 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2508 5738 5912 65
Size: 1183 1260 3230 174 1396
Fragments arranged by size:
3230 1396 1260 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5887 6074 1700
Size: 110 4077 187 2869
Fragments arranged by size:
4077 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2122 96
Size: 2026 5217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7243
BssSI C'ACGA_G
Cuts at: 3066 5303 6315 3066
Size: 2237 1012 3994
Fragments arranged by size:
3994 2237 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7243
BstBI TT'CG_AA
Cuts at: 6047 6047
Size: 7243
BstEII G'GTnAC_C
Cuts at: 1184 4402 1184
Size: 3218 4025
BstXI CCAn_nnnn'nTGG
Cuts at: 2330 2330
Size: 7243
BstYI r'GATC_y
Cuts at: 1972 2969 4573 4940 5325 5342 6794 6880 6892
Size: 997 1604 367 385 17 1452 86 12
Cuts at: 6892 7238 1972
Size: 346 1977
Fragments arranged by size:
1977 1604 1452 997 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2039 2039
Size: 7243
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2455 3656 513
Size: 552 939 24 427 1201 4100
Fragments arranged by size:
4100 1201 939 552 427 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2475 487
Size: 683 56 1249 5255
Fragments arranged by size:
5255 1249 683 56
BtsI GCAGTG_nn'
Cuts at: 4759 5553 5573 4759
Size: 794 20 6429
Fragments arranged by size:
6429 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3860 3895 529
Size: 35 3296 35 3877
Fragments arranged by size:
3877 3296 35 35
DraI TTT'AAA
Cuts at: 4547 4547
Size: 7243
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4460 6250 809
Size: 507 3144 1790 1802
Fragments arranged by size:
3144 1802 1790 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4690 5579 7208
Size: 54 1425 50 275 18 2860 889 1629
Cuts at: 7208 8
Size: 43
Fragments arranged by size:
2860 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4690 1537
Size: 275 2878 4090
Fragments arranged by size:
4090 2878 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2690 5173 1353
Size: 1337 2483 3423
Fragments arranged by size:
3423 2483 1337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2059 2316 5830 6204 6350 387
Size: 1064 138 470 257 3514 374 146 1280
Fragments arranged by size:
3514 1280 1064 470 374 257 146 138
EcoICRI GAG'CTC
Cuts at: 3278 3278
Size: 7243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2630 2937 2991 3231 3612 3935
Size: 393 558 440 307 54 240 381 323
Cuts at: 3935 5306 5903 6690 6958 1239
Size: 1371 597 787 268 1524
Fragments arranged by size:
1524 1371 787 597 558 440 393 381
323 307 268 240 54
EcoO109I rG'GnC_Cy
Cuts at: 2011 2011
Size: 7243
EcoRI G'AATT_C
Cuts at: 4552 4930 4552
Size: 378 6865
EcoRV GAT'ATC
Cuts at: 2425 2605 2425
Size: 180 7063
FspI TGC'GCA
Cuts at: 5749 5749
Size: 7243
HaeII r_GCGC'y
Cuts at: 1678 6390 1678
Size: 4712 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5884 5916 5958 5990 1373
Size: 32 135 32 4312 32 42 32 2626
Fragments arranged by size:
4312 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4587 4833 669
Size: 988 281 2649 246 3079
Fragments arranged by size:
3079 2649 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4384 748
Size: 3636 3607
HpaI GTT'AAC
Cuts at: 4833 4833
Size: 7243
KpnI G_GTAC'C
Cuts at: 4568 4568
Size: 7243
MfeI C'AATT_G
Cuts at: 2628 4842 2628
Size: 2214 5029
MluI A'CGCG_T
Cuts at: 7119 7119
Size: 7243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4185 6357 6541 1305
Size: 2880 2172 184 2007
Fragments arranged by size:
2880 2172 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5421
Fragments arranged by size:
5421 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2460 3661 4144 5201 5560 5719 518
Size: 648 1294 1201 483 1057 359 159 2042
Fragments arranged by size:
2042 1294 1201 1057 648 483 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2030 2088 4659 5336 6484 6729 1606
Size: 411 13 58 2571 677 1148 245 2120
Fragments arranged by size:
2571 2120 1148 677 411 245 58 13
NcoI C'CATG_G
Cuts at: 513 1065 2004 2455 513
Size: 552 939 451 5301
Fragments arranged by size:
5301 939 552 451
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7243
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7243
NotI GC'GGCC_GC
Cuts at: 4690 4690
Size: 7243
NsiI A_TGCA'T
Cuts at: 2418 2418
Size: 7243
NspI r_CATG'y
Cuts at: 1132 1229 3495 3764 4601 6146 1132
Size: 97 2266 269 837 1545 2229
Fragments arranged by size:
2266 2229 1545 837 269 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7243
PciI A'CATG_T
Cuts at: 6142 6142
Size: 7243
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3314 1108
Size: 2206 5037
PfoI T'CCnGG_A
Cuts at: 3545 3545
Size: 7243
PmeI GTTT'AAAC
Cuts at: 4547 4547
Size: 7243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5312 5344 6825 6857 5312
Size: 32 1481 32 5698
Fragments arranged by size:
5698 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7243
PsiI TTA'TAA
Cuts at: 4813 4813
Size: 7243
PstI C_TGCA'G
Cuts at: 830 2146 4595 830
Size: 1316 2449 3478
Fragments arranged by size:
3478 2449 1316
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7243
SacI G_AGCT'C
Cuts at: 3280 3280
Size: 7243
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 7243
SalI G'TCGA_C
Cuts at: 1936 4585 1936
Size: 2649 4594
SbfI CC_TGCA'GG
Cuts at: 4595 4595
Size: 7243
ScaI AGT'ACT
Cuts at: 5491 7203 5491
Size: 1712 5531
SfcI C'TryA_G
Cuts at: 826 1045 2142 4326 4591 5726 6407 6598 826
Size: 219 1097 2184 265 1135 681 191 1471
Fragments arranged by size:
2184 1471 1135 1097 681 265 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7243
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7243
SmaI CCC'GGG
Cuts at: 1872 4570 1872
Size: 2698 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5347 6248 6510 820
Size: 197 926 3404 901 262 1553
Fragments arranged by size:
3404 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5034 493
Size: 4541 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7243
SphI G_CATG'C
Cuts at: 1132 3495 4601 1132
Size: 2363 1106 3774
Fragments arranged by size:
3774 2363 1106
SspI AAT'ATT
Cuts at: 5 52 2202 2440 5167 5
Size: 47 2150 238 2727 2081
Fragments arranged by size:
2727 2150 2081 238 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7243
StyI C'CwwG_G
Cuts at: 513 1065 2004 2455 3191 4682 513
Size: 552 939 451 736 1491 3074
Fragments arranged by size:
3074 1491 939 736 552 451
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2685 3481 3828 5270 5287 5440 5625
Size: 1255 1105 796 347 1442 17 153 185
Cuts at: 5625 6070 325
Size: 445 1498
Fragments arranged by size:
1498 1442 1255 1105 796 445 347 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2122 3573 3665
Size: 274 80 33 51 1443 145 1451 92
Cuts at: 3665 5489 7201 96
Size: 1824 1712 138
Fragments arranged by size:
1824 1712 1451 1443 274 145 138 92
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7203 7235 7203
Size: 32 7211
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7243
XbaI T'CTAG_A
Cuts at: 2852 4579 2852
Size: 1727 5516
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7243
XmaI C'CCGG_G
Cuts at: 1870 4568 1870
Size: 2698 4545
XmnI GAAnn'nnTTC
Cuts at: 2814 5372 2814
Size: 2558 4685
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2104 5051 276
Size: 53 83 186 936 570 2947 2468
Fragments arranged by size:
2947 2468 936 570 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI