Restriction map for
pFN21AB8475
(Circular) (Six-base) MAPSORT of: pFN21AB8475.seq Check: 4329 from: 1 to: 7354
pFN21AB8475 7354 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3055 5164 278
Size: 53 83 186 936 1519 2109 2468
Fragments arranged by size:
2468 2109 1519 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4276 4697 1937
Size: 2339 421 4594
Fragments arranged by size:
4594 2339 421
Acc65I G'GTAC_C
Cuts at: 4675 4675
Size: 7354
AclI AA'CG_TT
Cuts at: 1563 5481 5854 1563
Size: 3918 373 3063
Fragments arranged by size:
3918 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3824 5417 6801 7069 1632
Size: 2192 1593 1384 268 1917
Fragments arranged by size:
2192 1917 1593 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3740 4091 820
Size: 197 2723 351 4083
Fragments arranged by size:
4083 2723 351 197
AflIII A'CryG_T
Cuts at: 1203 3096 4279 6253 7230 1203
Size: 1893 1183 1974 977 1327
Fragments arranged by size:
1974 1893 1327 1183 977
AgeI A'CCGG_T
Cuts at: 6185 6185
Size: 7354
AhdI GACnn_n'nnGTC
Cuts at: 6083 6083
Size: 7354
AleI CACnn'nnGTG
Cuts at: 1166 2517 3966 1166
Size: 1351 1449 4554
Fragments arranged by size:
4554 1449 1351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7354
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3675 3707 3675
Size: 32 7322
AlwNI CAG_nnn'CTG
Cuts at: 4310 5097 6669 4310
Size: 787 1572 4995
Fragments arranged by size:
4995 1572 787
ApaLI G'TGCA_C
Cuts at: 5411 6567 5411
Size: 1156 6198
ApoI r'AATT_y
Cuts at: 1493 2255 2279 2588 2705 3861 4663 4889 5041
Size: 762 24 309 117 1156 802 226 152
Cuts at: 5041 7150 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1156 802 762 309 226 152
117 24
AseI AT'TA_AT
Cuts at: 160 1032 3825 5908 160
Size: 872 2793 2083 1606
Fragments arranged by size:
2793 2083 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7354
AvaI C'yCGr_G
Cuts at: 1870 1943 2946 4679 1870
Size: 73 1003 1733 4545
Fragments arranged by size:
4545 1733 1003 73
BamHI G'GATC_C
Cuts at: 4684 5051 4684
Size: 367 6987
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4675 6130 618
Size: 325 217 627 2888 1455 1842
Fragments arranged by size:
2888 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2997 4462 4474 4673 1391
Size: 1606 1465 12 199 4072
Fragments arranged by size:
4072 1606 1465 199 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3219 3477 928
Size: 971 1320 258 4805
Fragments arranged by size:
4805 1320 971 258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5545 5579 1903
Size: 34 3608 34 3678
Fragments arranged by size:
3678 3608 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4580 5246 6456 4580
Size: 666 1210 5478
Fragments arranged by size:
5478 1210 666
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7354
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5965 136
Size: 107 122 71 631 605 4293 1525
Fragments arranged by size:
4293 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2869 4634 7349 2869
Size: 1765 2715 2874
Fragments arranged by size:
2874 2715 1765
BlpI GC'TnA_GC
Cuts at: 3075 3132 4771 3075
Size: 57 1639 5658
Fragments arranged by size:
5658 1639 57
Bme1580I G_kGCm'C
Cuts at: 1790 5415 6571 1790
Size: 3625 1156 2573
Fragments arranged by size:
3625 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3097 1318
Size: 1779 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2046 3708 5085
Size: 416 53 83 399 24 629 1662 1377
Cuts at: 5085 6043 442
Size: 958 1753
Fragments arranged by size:
1753 1662 1377 958 629 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2973 3228 6014 1239
Size: 1734 255 2786 2579
Fragments arranged by size:
2786 2579 1734 255
Bpu10I CC'TnA_GC
Cuts at: 2991 2991
Size: 7354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2981 3620 5479 6344 6642 2981
Size: 639 1859 865 298 3693
Fragments arranged by size:
3693 1859 865 639 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6017 882
Size: 617 4518 2219
Fragments arranged by size:
4518 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5145 493
Size: 711 3941 2702
Fragments arranged by size:
3941 2702 711
BsaBI GATnn'nnATC
Cuts at: 3762 7348 3762
Size: 3586 3768
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3052 5161 5543 6178
Size: 53 83 186 936 1519 2109 382 635
Cuts at: 6178 275
Size: 1451
Fragments arranged by size:
2109 1519 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2275 5787 6185 6459 6606 7017 2275
Size: 3512 398 274 147 411 2612
Fragments arranged by size:
3512 2612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3538 3568 3969 3999 1178
Size: 30 165 30 2135 30 401 30 4533
Fragments arranged by size:
4533 2135 401 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3413 3990 1186
Size: 2227 577 4550
Fragments arranged by size:
4550 2227 577
BseYI C'CCAG_C
Cuts at: 3895 4081 6557 7053 3895
Size: 186 2476 496 4196
Fragments arranged by size:
4196 2476 496 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6781
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4804 5565 6593 1540
Size: 275 186 2803 761 1028 2301
Fragments arranged by size:
2803 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2997 3963 4462 5415 5500 6571 2997
Size: 966 499 953 85 1071 3780
Fragments arranged by size:
3780 1071 966 953 499 85
BsmI GAATG_Cn'
Cuts at: 2931 3136 4430 4865 4958 2931
Size: 205 1294 435 93 5327
Fragments arranged by size:
5327 1294 435 205 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2997 3963 4462 4474 4673 5415 5500
Size: 399 1207 966 499 12 199 742 85
Cuts at: 5500 6571 1391
Size: 1071 2174
Fragments arranged by size:
2174 1207 1071 966 742 499 399 199
85 12
BspEI T'CCGG_A
Cuts at: 2275 7017 2275
Size: 4742 2612
BspHI T'CATG_A
Cuts at: 5243 6973 5243
Size: 1730 5624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4556 4709 844
Size: 3712 153 3489
Fragments arranged by size:
3712 3489 153
BsrBI CCG'CTC
Cuts at: 1394 1823 5241 1394
Size: 429 3418 3507
Fragments arranged by size:
3507 3418 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4525 5849 6023 65
Size: 1183 3277 1324 174 1396
Fragments arranged by size:
3277 1396 1324 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5998 6185 1700
Size: 110 4188 187 2869
Fragments arranged by size:
4188 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2429 2573 3442 96
Size: 2333 144 869 4008
Fragments arranged by size:
4008 2333 869 144
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7354
BssSI C'ACGA_G
Cuts at: 5414 6426 5414
Size: 1012 6342
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7354
BstBI TT'CG_AA
Cuts at: 2530 4465 6158 2530
Size: 1935 1693 3726
Fragments arranged by size:
3726 1935 1693
BstEII G'GTnAC_C
Cuts at: 1184 3708 1184
Size: 2524 4830
BstYI r'GATC_y
Cuts at: 1972 2114 2869 4634 4684 5051 5436 5453 6905
Size: 142 755 1765 50 367 385 17 1452
Cuts at: 6905 6991 7003 7349 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1765 1452 755 385 367 346 142
86 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4486 513
Size: 552 939 2482 3381
Fragments arranged by size:
3381 2482 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6615
Fragments arranged by size:
6615 683 56
BtsI GCAGTG_nn'
Cuts at: 4870 5664 5684 4870
Size: 794 20 6540
Fragments arranged by size:
6540 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7319
DraI TTT'AAA
Cuts at: 2710 3060 3732 4658 2710
Size: 350 672 926 5406
Fragments arranged by size:
5406 926 672 350
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3097 6361 809
Size: 507 1781 3264 1802
Fragments arranged by size:
3264 1802 1781 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4066 4801 5690
Size: 54 1425 50 275 18 2236 735 889
Cuts at: 5690 7319 8
Size: 1629 43
Fragments arranged by size:
2236 1629 1425 889 735 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4801 1537
Size: 275 2989 4090
Fragments arranged by size:
4090 2989 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2065 2130 2757 2847 3354 3998 4004 4467
Size: 712 65 627 90 507 644 6 463
Cuts at: 4467 5284 1353
Size: 817 3423
Fragments arranged by size:
3423 817 712 644 627 507 463 90
65 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2674 5941 6315 6461 387
Size: 1064 138 1085 3267 374 146 1280
Fragments arranged by size:
3267 1280 1085 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2995 4460 2995
Size: 1465 5889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2973 3228 3824 5417 6014 6801 7069
Size: 393 1341 255 596 1593 597 787 268
Cuts at: 7069 1239
Size: 1524
Fragments arranged by size:
1593 1524 1341 787 597 596 393 268
255
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 2336
Size: 7354
EcoRI G'AATT_C
Cuts at: 4663 5041 4663
Size: 378 6976
EcoRV GAT'ATC
Cuts at: 3166 3433 3166
Size: 267 7087
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2213 3086 4527 2119
Size: 94 873 1441 4946
Fragments arranged by size:
4946 1441 873 94
FspI TGC'GCA
Cuts at: 5860 5860
Size: 7354
HaeII r_GCGC'y
Cuts at: 1678 3124 6501 1678
Size: 1446 3377 2531
Fragments arranged by size:
3377 2531 1446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4258 4290 4325 4357 5995
Size: 32 135 32 2686 32 35 32 1638
Cuts at: 5995 6027 6069 6101 1373
Size: 32 42 32 2626
Fragments arranged by size:
2686 2626 1638 135 42 35 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4277 4698 4944 669
Size: 988 281 2339 421 246 3079
Fragments arranged by size:
3079 2339 988 421 281 246
HindIII A'AGCT_T
Cuts at: 748 2646 3922 748
Size: 1898 1276 4180
Fragments arranged by size:
4180 1898 1276
HpaI GTT'AAC
Cuts at: 4944 4944
Size: 7354
KpnI G_GTAC'C
Cuts at: 4679 4679
Size: 7354
MfeI C'AATT_G
Cuts at: 4953 4953
Size: 7354
MluI A'CGCG_T
Cuts at: 7230 7230
Size: 7354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3195 3718 6468 6652 1305
Size: 1890 523 2750 184 2007
Fragments arranged by size:
2750 2007 1890 523 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4068 10
Size: 54 1425 343 2236 3296
Fragments arranged by size:
3296 2236 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2410 2517 2936 3825 3966 5312
Size: 648 1056 188 107 419 889 141 1346
Cuts at: 5312 5671 5830 518
Size: 359 159 2042
Fragments arranged by size:
2042 1346 1056 889 648 419 359 188
159 141 107
MspA1I CmG'CkG
Cuts at: 1606 3131 3755 3934 4770 5447 6595 6840 1606
Size: 1525 624 179 836 677 1148 245 2120
Fragments arranged by size:
2120 1525 1148 836 677 624 245 179
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5863
Fragments arranged by size:
5863 939 552
NdeI CA'TA_TG
Cuts at: 387 2125 387
Size: 1738 5616
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7354
NotI GC'GGCC_GC
Cuts at: 4801 4801
Size: 7354
NspI r_CATG'y
Cuts at: 1132 1229 4283 4532 4712 6257 1132
Size: 97 3054 249 180 1545 2229
Fragments arranged by size:
3054 2229 1545 249 180 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7354
PciI A'CATG_T
Cuts at: 4279 6253 4279
Size: 1974 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2515 3662 1108
Size: 1407 1147 4800
Fragments arranged by size:
4800 1407 1147
PmeI GTTT'AAAC
Cuts at: 4658 4658
Size: 7354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5423 5455 6936 6968 5423
Size: 32 1481 32 5809
Fragments arranged by size:
5809 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2504 1613
Size: 891 6463
PsiI TTA'TAA
Cuts at: 2201 2643 4924 2201
Size: 442 2281 4631
Fragments arranged by size:
4631 2281 442
PstI C_TGCA'G
Cuts at: 830 4706 830
Size: 3876 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7354
PvuII CAG'CTG
Cuts at: 3131 3755 3131
Size: 624 6730
SacI G_AGCT'C
Cuts at: 2997 4462 2997
Size: 1465 5889
SalI G'TCGA_C
Cuts at: 1936 4275 4696 1936
Size: 2339 421 4594
Fragments arranged by size:
4594 2339 421
SapI GCTCTTCn'nnn_
Cuts at: 4467 4467
Size: 7354
SbfI CC_TGCA'GG
Cuts at: 4706 4706
Size: 7354
ScaI AGT'ACT
Cuts at: 2451 5602 7314 2451
Size: 3151 1712 2491
Fragments arranged by size:
3151 2491 1712
SfcI C'TryA_G
Cuts at: 826 1045 4702 5837 6518 6709 826
Size: 219 3657 1135 681 191 1471
Fragments arranged by size:
3657 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7354
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7354
SmaI CCC'GGG
Cuts at: 1872 4681 1872
Size: 2809 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2996 3635 3740 4091 5458 6359
Size: 197 926 1053 639 105 351 1367 901
Cuts at: 6359 6621 820
Size: 262 1553
Fragments arranged by size:
1553 1367 1053 926 901 639 351 262
197 105
SnaBI TAC'GTA
Cuts at: 493 5145 493
Size: 4652 2702
SpeI A'CTAG_T
Cuts at: 152 2485 152
Size: 2333 5021
SphI G_CATG'C
Cuts at: 1132 4712 1132
Size: 3580 3774
SspI AAT'ATT
Cuts at: 5 52 2810 3527 5278 5
Size: 47 2758 717 1751 2081
Fragments arranged by size:
2758 2081 1751 717 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7354
StyI C'CwwG_G
Cuts at: 513 1065 2004 2094 4793 513
Size: 552 939 90 2699 3074
Fragments arranged by size:
3074 2699 939 552 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2507 5381 5398 5551 5736 6181 325
Size: 1255 927 2874 17 153 185 445 1498
Fragments arranged by size:
2874 1498 1255 927 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2429 2449 2573
Size: 274 80 33 51 1443 452 20 124
Cuts at: 2573 3218 3442 3700 3928 4373 5600 7312 96
Size: 645 224 258 228 445 1227 1712 138
Fragments arranged by size:
1712 1443 1227 645 452 445 274 258
228 224 138 124 80 51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3232 3264 7314 7346 3232
Size: 32 4050 32 3240
Fragments arranged by size:
4050 3240 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7354
XbaI T'CTAG_A
Cuts at: 4690 4690
Size: 7354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2516 4254 2516
Size: 1738 5616
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7354
XmaI C'CCGG_G
Cuts at: 1870 4679 1870
Size: 2809 4545
XmnI GAAnn'nnTTC
Cuts at: 4510 5483 4510
Size: 973 6381
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3053 5162 276
Size: 53 83 186 936 1519 2109 2468
Fragments arranged by size:
2468 2109 1519 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI