Restriction map for
pFN21AB8472
(Circular) (Six-base) MAPSORT of: pFN21AB8472.seq Check: 6254 from: 1 to: 7225
pFN21AB8472 7225 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5035 278
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2607 4568 1937
Size: 670 1961 4594
Fragments arranged by size:
4594 1961 670
Acc65I G'GTAC_C
Cuts at: 4546 4546
Size: 7225
AclI AA'CG_TT
Cuts at: 1563 5352 5725 1563
Size: 3789 373 3063
Fragments arranged by size:
3789 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2832 3255 3663 4101 5288 6672 6940
Size: 767 433 423 408 438 1187 1384 268
Cuts at: 6940 1632
Size: 1917
Fragments arranged by size:
1917 1384 1187 767 438 433 423 408
268
AflII C'TTAA_G
Cuts at: 820 1017 2402 820
Size: 197 1385 5643
Fragments arranged by size:
5643 1385 197
AflIII A'CryG_T
Cuts at: 1203 3592 6124 7101 1203
Size: 2389 2532 977 1327
Fragments arranged by size:
2532 2389 1327 977
AgeI A'CCGG_T
Cuts at: 6056 6056
Size: 7225
AhdI GACnn_n'nnGTC
Cuts at: 2773 5954 2773
Size: 3181 4044
AleI CACnn'nnGTG
Cuts at: 1166 2736 4478 1166
Size: 1570 1742 3913
Fragments arranged by size:
3913 1742 1570
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2373 1184
Size: 1189 6036
AlwNI CAG_nnn'CTG
Cuts at: 3374 3641 4968 6540 3374
Size: 267 1327 1572 4059
Fragments arranged by size:
4059 1572 1327 267
ApaLI G'TGCA_C
Cuts at: 5282 6438 5282
Size: 1156 6069
ApoI r'AATT_y
Cuts at: 1493 2335 2708 2744 2918 3071 3176 3223 4534
Size: 842 373 36 174 153 105 47 1311
Cuts at: 4534 4760 4912 7021 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1311 842 373 226 174 153
152 105 47 36
AseI AT'TA_AT
Cuts at: 160 1032 2513 5779 160
Size: 872 1481 3266 1606
Fragments arranged by size:
3266 1606 1481 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
AvaI C'yCGr_G
Cuts at: 1870 1943 3123 4550 1870
Size: 73 1180 1427 4545
Fragments arranged by size:
4545 1427 1180 73
BamHI G'GATC_C
Cuts at: 3668 4282 4555 4922 3668
Size: 614 273 367 5971
Fragments arranged by size:
5971 614 367 273
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4546 6001 618
Size: 325 217 627 2759 1455 1842
Fragments arranged by size:
2759 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4544 1391
Size: 3153 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3728 4002 928
Size: 971 1829 274 4151
Fragments arranged by size:
4151 1829 971 274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3058 3092 5416 5450 1903
Size: 34 1121 34 2324 34 3678
Fragments arranged by size:
3678 2324 1121 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5117 6327 5117
Size: 1210 6015
BclI T'GATC_A
Cuts at: 1548 3816 4174 1548
Size: 2268 358 4599
Fragments arranged by size:
4599 2268 358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3710 5836 136
Size: 107 122 71 631 605 2038 2126 1525
Fragments arranged by size:
2126 2038 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2851 7220 2851
Size: 4369 2856
BlpI GC'TnA_GC
Cuts at: 4458 4642 4458
Size: 184 7041
Bme1580I G_kGCm'C
Cuts at: 1790 5286 6442 1790
Size: 3496 1156 2573
Fragments arranged by size:
3496 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7225
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4956 5914 442
Size: 416 53 83 399 24 3539 958 1753
Fragments arranged by size:
3539 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7225
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3800
Size: 7225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2945 3213 3409 3854 5885 1239
Size: 1706 268 196 445 2031 2579
Fragments arranged by size:
2579 2031 1706 445 268 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2807 3350 3645 5350 6215 6513 2807
Size: 543 295 1705 865 298 3519
Fragments arranged by size:
3519 1705 865 543 298 295
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3647 5888 882
Size: 617 2148 2241 2219
Fragments arranged by size:
2241 2219 2148 617
BsaAI yAC'GTr
Cuts at: 493 1204 5016 493
Size: 711 3812 2702
Fragments arranged by size:
3812 2702 711
BsaBI GATnn'nnATC
Cuts at: 7219 7219
Size: 7225
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5032 5414 6049 275
Size: 53 83 186 936 3499 382 635 1451
Fragments arranged by size:
3499 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5658 6056 6330 6477 6888 5658
Size: 398 274 147 411 5995
Fragments arranged by size:
5995 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4154 4184 1178
Size: 30 165 30 2751 30 4219
Fragments arranged by size:
4219 2751 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3339 3399 3539 1186
Size: 2153 60 140 4872
Fragments arranged by size:
4872 2153 140 60
BseYI C'CCAG_C
Cuts at: 6428 6924 6428
Size: 496 6729
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 2300 1163
Size: 573 353 211 6088
Fragments arranged by size:
6088 573 353 211
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2101 4675 5436 6464 1540
Size: 275 186 100 2574 761 1028 2301
Fragments arranged by size:
2574 2301 1028 761 275 186 100
BsiHKAI G_wGCw'C
Cuts at: 5286 5371 6442 5286
Size: 85 1071 6069
Fragments arranged by size:
6069 1071 85
BsmI GAATG_Cn'
Cuts at: 2225 3553 4736 4829 2225
Size: 1328 1183 93 4621
Fragments arranged by size:
4621 1328 1183 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4544 5286 5371 6442 1391
Size: 399 2754 742 85 1071 2174
Fragments arranged by size:
2754 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6888 6888
Size: 7225
BspHI T'CATG_A
Cuts at: 5114 6844 5114
Size: 1730 5495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3747 4580 844
Size: 2903 833 3489
Fragments arranged by size:
3489 2903 833
BsrBI CCG'CTC
Cuts at: 1394 1823 3844 5112 1394
Size: 429 2021 1268 3507
Fragments arranged by size:
3507 2021 1268 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2941 3134 4233 5720 5894 65
Size: 1183 1693 193 1099 1487 174 1396
Fragments arranged by size:
1693 1487 1396 1183 1099 193 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2063 2110 5869 6056 1700
Size: 110 253 47 3759 187 2869
Fragments arranged by size:
3759 2869 253 187 110 47
BsrGI T'GTAC_A
Cuts at: 96 3792 96
Size: 3696 3529
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7225
BssSI C'ACGA_G
Cuts at: 5285 6297 5285
Size: 1012 6213
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7225
BstBI TT'CG_AA
Cuts at: 6029 6029
Size: 7225
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7225
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3733 2011
Size: 1722 5503
BstYI r'GATC_y
Cuts at: 1972 2563 2851 3370 3668 4119 4282 4555 4922
Size: 591 288 519 298 451 163 273 367
Cuts at: 4922 5307 5324 6776 6862 6874 7220 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 591 519 451 385 367 346
298 288 273 163 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3351 3351
Size: 7225
BtgI C'CryG_G
Cuts at: 513 1065 2215 3463 4278 513
Size: 552 1150 1248 815 3460
Fragments arranged by size:
3460 1248 1150 815 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6486
Fragments arranged by size:
6486 683 56
BtsI GCAGTG_nn'
Cuts at: 2526 2676 3021 3146 3870 4741 5535 5555 2526
Size: 150 345 125 724 871 794 20 4196
Fragments arranged by size:
4196 871 794 724 345 150 125 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2515 2550 2665 2700 3883 3918 529
Size: 35 1951 35 115 35 1183 35 3836
Fragments arranged by size:
3836 1951 1183 115 35 35 35 35
DraI TTT'AAA
Cuts at: 2206 4529 2206
Size: 2323 4902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3257 6232 809
Size: 507 1941 2975 1802
Fragments arranged by size:
2975 1941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2218 2673 4672
Size: 54 1425 50 275 18 388 455 1999
Cuts at: 4672 5561 7190 8
Size: 889 1629 43
Fragments arranged by size:
1999 1629 1425 889 455 388 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4672 1537
Size: 275 2860 4090
Fragments arranged by size:
4090 2860 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2322 2574 3555 3594 5155 1353
Size: 969 252 981 39 1561 3423
Fragments arranged by size:
3423 1561 981 969 252 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5812 6186 6332 387
Size: 1064 138 4223 374 146 1280
Fragments arranged by size:
4223 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2832 2945 3213 3255 3409 3663
Size: 393 767 433 113 268 42 154 254
Cuts at: 3663 3854 4101 5288 5885 6672 6940 1239
Size: 191 247 1187 597 787 268 1524
Fragments arranged by size:
1524 1187 787 767 597 433 393 268
268 254 247 191 154 113 42
EcoO109I rG'GnC_Cy
Cuts at: 4445 4445
Size: 7225
EcoRI G'AATT_C
Cuts at: 3223 4534 4912 3223
Size: 1311 378 5536
Fragments arranged by size:
5536 1311 378
FspI TGC'GCA
Cuts at: 5731 5731
Size: 7225
HaeII r_GCGC'y
Cuts at: 1678 6372 1678
Size: 4694 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2479 2511 2759 2791 3444
Size: 32 135 32 907 32 248 32 653
Cuts at: 3444 3476 4267 4299 5866 5898 5940 5972 1373
Size: 32 791 32 1567 32 42 32 2626
Fragments arranged by size:
2626 1567 907 791 653 248 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2419 4052 4569 4815 669
Size: 988 281 481 1633 517 246 3079
Fragments arranged by size:
3079 1633 988 517 481 281 246
HindIII A'AGCT_T
Cuts at: 748 3896 4502 748
Size: 3148 606 3471
Fragments arranged by size:
3471 3148 606
HpaI GTT'AAC
Cuts at: 4815 4815
Size: 7225
KpnI G_GTAC'C
Cuts at: 4550 4550
Size: 7225
MfeI C'AATT_G
Cuts at: 2145 4824 2145
Size: 2679 4546
MluI A'CGCG_T
Cuts at: 7101 7101
Size: 7225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4147 6339 6523 1305
Size: 2842 2192 184 2007
Fragments arranged by size:
2842 2192 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 10
Size: 54 1425 343 843 4560
Fragments arranged by size:
4560 1425 843 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2736 3139 4182 4478 5183 5542
Size: 648 843 727 403 1043 296 705 359
Cuts at: 5542 5701 518
Size: 159 2042
Fragments arranged by size:
2042 1043 843 727 705 648 403 359
296 159
MspA1I CmG'CkG
Cuts at: 1606 2103 3233 4478 4641 5318 6466 6711 1606
Size: 497 1130 1245 163 677 1148 245 2120
Fragments arranged by size:
2120 1245 1148 1130 677 497 245 163
NaeI GCC'GGC
Cuts at: 2065 2112 2065
Size: 47 7178
NcoI C'CATG_G
Cuts at: 513 1065 2215 3463 4278 513
Size: 552 1150 1248 815 3460
Fragments arranged by size:
3460 1248 1150 815 552
NdeI CA'TA_TG
Cuts at: 387 2269 2545 387
Size: 1882 276 5067
Fragments arranged by size:
5067 1882 276
NgoMIV G'CCGG_C
Cuts at: 2063 2110 2063
Size: 47 7178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7225
NotI GC'GGCC_GC
Cuts at: 4672 4672
Size: 7225
NspI r_CATG'y
Cuts at: 1132 1229 3596 4583 6128 1132
Size: 97 2367 987 1545 2229
Fragments arranged by size:
2367 2229 1545 987 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7225
PciI A'CATG_T
Cuts at: 3592 6124 3592
Size: 2532 4693
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4424 1108
Size: 3316 3909
PfoI T'CCnGG_A
Cuts at: 4071 4071
Size: 7225
PmeI GTTT'AAAC
Cuts at: 4529 4529
Size: 7225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5294 5326 6807 6839 5294
Size: 32 1481 32 5680
Fragments arranged by size:
5680 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7225
PsiI TTA'TAA
Cuts at: 4795 4795
Size: 7225
PspXI vC'TCGA_Gb
Cuts at: 3123 3123
Size: 7225
PstI C_TGCA'G
Cuts at: 830 2533 4260 4577 830
Size: 1703 1727 317 3478
Fragments arranged by size:
3478 1727 1703 317
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7225
PvuII CAG'CTG
Cuts at: 3233 4478 3233
Size: 1245 5980
SalI G'TCGA_C
Cuts at: 1936 4567 1936
Size: 2631 4594
SapI GCTCTTCn'nnn_
Cuts at: 2322 3594 2322
Size: 1272 5953
SbfI CC_TGCA'GG
Cuts at: 4577 4577
Size: 7225
ScaI AGT'ACT
Cuts at: 3730 4336 5473 7185 3730
Size: 606 1137 1712 3770
Fragments arranged by size:
3770 1712 1137 606
SfcI C'TryA_G
Cuts at: 826 1045 2289 2412 2529 3340 3378 4256 4573
Size: 219 1244 123 117 811 38 878 317
Cuts at: 4573 5708 6389 6580 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1244 1135 878 811 681 317 219
191 123 117 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
SgrAI Cr'CCGG_yG
Cuts at: 1700 2063 1700
Size: 363 6862
SmaI CCC'GGG
Cuts at: 1872 4552 1872
Size: 2680 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2402 2822 3123 3329 3660 5329
Size: 197 926 459 420 301 206 331 1669
Cuts at: 5329 6230 6492 820
Size: 901 262 1553
Fragments arranged by size:
1669 1553 926 901 459 420 331 301
262 206 197
SnaBI TAC'GTA
Cuts at: 493 5016 493
Size: 4523 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7225
SphI G_CATG'C
Cuts at: 1132 4583 1132
Size: 3451 3774
SspI AAT'ATT
Cuts at: 5 52 5149 5
Size: 47 5097 2081
Fragments arranged by size:
5097 2081 47
StuI AGG'CCT
Cuts at: 1517 3335 1517
Size: 1818 5407
StyI C'CwwG_G
Cuts at: 513 1065 2197 2215 3463 3672 4278 4664 513
Size: 552 1132 18 1248 209 606 386 3074
Fragments arranged by size:
3074 1248 1132 606 552 386 209 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5252 5269 5422 5607 6052 325
Size: 1255 3672 17 153 185 445 1498
Fragments arranged by size:
3672 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3092 3728 3792
Size: 274 80 33 51 1443 1115 636 64
Cuts at: 3792 4334 5471 7183 96
Size: 542 1137 1712 138
Fragments arranged by size:
1712 1443 1137 1115 636 542 274 138
80 64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7185 7217 7185
Size: 32 7193
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7225
XbaI T'CTAG_A
Cuts at: 4561 4561
Size: 7225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 4369 2011
Size: 2358 4867
XhoI C'TCGA_G
Cuts at: 1943 3123 1943
Size: 1180 6045
XmaI C'CCGG_G
Cuts at: 1870 4550 1870
Size: 2680 4545
XmnI GAAnn'nnTTC
Cuts at: 2397 5354 2397
Size: 2957 4268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5033 276
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI