Restriction map for
pFN21AB8471
(Circular) (Six-base) MAPSORT of: pFN21AB8471.seq Check: 1848 from: 1 to: 7441
pFN21AB8471 7441 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3115 3660 3115
Size: 545 6896
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4065 4635 5251 278
Size: 53 83 186 936 2529 570 616 2468
Fragments arranged by size:
2529 2468 936 616 570 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4784 1937
Size: 2847 4594
Acc65I G'GTAC_C
Cuts at: 3773 4762 3773
Size: 989 6452
AclI AA'CG_TT
Cuts at: 1563 5568 5941 1563
Size: 4005 373 3063
Fragments arranged by size:
4005 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2403 2991 3974 4739 5504 6888 7156 1632
Size: 771 588 983 765 765 1384 268 1917
Fragments arranged by size:
1917 1384 983 771 765 765 588 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7244
AflIII A'CryG_T
Cuts at: 1203 3318 3756 6340 7317 1203
Size: 2115 438 2584 977 1327
Fragments arranged by size:
2584 2115 1327 977 438
AgeI A'CCGG_T
Cuts at: 6272 6272
Size: 7441
AhdI GACnn_n'nnGTC
Cuts at: 6170 6170
Size: 7441
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7441
AlwNI CAG_nnn'CTG
Cuts at: 4131 4666 5184 6756 4131
Size: 535 518 1572 4816
Fragments arranged by size:
4816 1572 535 518
ApaI G_GGCC'C
Cuts at: 2347 2703 3235 4103 4378 2347
Size: 356 532 868 275 5410
Fragments arranged by size:
5410 868 532 356 275
ApaLI G'TGCA_C
Cuts at: 4585 5498 6654 4585
Size: 913 1156 5372
Fragments arranged by size:
5372 1156 913
ApoI r'AATT_y
Cuts at: 1493 3008 3851 4750 4976 5128 7237 1493
Size: 1515 843 899 226 152 2109 1697
Fragments arranged by size:
2109 1697 1515 899 843 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5995 160
Size: 872 4963 1606
Fragments arranged by size:
4963 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2502 2702 4322 4766 1870
Size: 73 87 472 200 1620 444 4545
Fragments arranged by size:
4545 1620 472 444 200 87 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3757 3790 3757
Size: 33 7408
BamHI G'GATC_C
Cuts at: 4771 5138 4771
Size: 367 7074
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 2975 3773 4032 4762
Size: 325 217 627 465 723 798 259 730
Cuts at: 4762 6217 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 798 730 723 627 465 325
259 217
BanII G_rGCy'C
Cuts at: 1391 2127 2301 2321 2347 2440 2501 2703 2796
Size: 736 174 20 26 93 61 202 93
Cuts at: 2796 3050 3235 3945 4103 4378 4760 1391
Size: 254 185 710 158 275 382 4072
Fragments arranged by size:
4072 736 710 382 275 254 202 185
174 158 93 93 61 26 20
BbeI G_GCGC'C
Cuts at: 2256 2256
Size: 7441
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3744 4546 928
Size: 971 1845 802 3823
Fragments arranged by size:
3823 1845 971 802
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5632 5666 1903
Size: 34 50 34 3611 34 3678
Fragments arranged by size:
3678 3611 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5333 6543 5333
Size: 1210 6231
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7441
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3264 3814 6052
Size: 107 122 71 631 605 1592 550 2238
Cuts at: 6052 136
Size: 1525
Fragments arranged by size:
2238 1592 1525 631 605 550 122 107
71
BglII A'GATC_T
Cuts at: 7436 7436
Size: 7441
BlpI GC'TnA_GC
Cuts at: 3044 4858 3044
Size: 1814 5627
Bme1580I G_kGCm'C
Cuts at: 1790 2347 2703 3235 3768 4103 4184 4378 4589
Size: 557 356 532 533 335 81 194 211
Cuts at: 4589 5502 6658 1790
Size: 913 1156 2573
Fragments arranged by size:
2573 1156 913 557 533 532 356 335
211 194 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7441
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4610 5172 6130
Size: 416 53 83 399 24 3193 562 958
Cuts at: 6130 442
Size: 1753
Fragments arranged by size:
3193 1753 958 562 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2005 3290 3590 3803 4463 4481 6101 1239
Size: 766 1285 300 213 660 18 1620 2579
Fragments arranged by size:
2579 1620 1285 766 660 300 213 18
Bpu10I CC'TnA_GC
Cuts at: 2790 2790
Size: 7441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2374 5566 6431 6729 2374
Size: 3192 865 298 3086
Fragments arranged by size:
3192 3086 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2366 2462 6104 882
Size: 617 867 96 3642 2219
Fragments arranged by size:
3642 2219 867 617 96
BsaAI yAC'GTr
Cuts at: 493 1204 3078 5232 493
Size: 711 1874 2154 2702
Fragments arranged by size:
2702 2154 1874 711
BsaBI GATnn'nnATC
Cuts at: 7435 7435
Size: 7441
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2253 4062 4632 5248
Size: 53 83 186 936 720 1809 570 616
Cuts at: 5248 5630 6265 275
Size: 382 635 1451
Fragments arranged by size:
1809 1451 936 720 635 616 570 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5874 6272 6546 6693 7104 5874
Size: 398 274 147 411 6211
Fragments arranged by size:
6211 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2876 2906 3563 3593 3794
Size: 30 165 30 1473 30 657 30 201
Cuts at: 3794 3824 1178
Size: 30 4795
Fragments arranged by size:
4795 1473 657 201 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2099 2108 2126 2324 2448 2738 1186
Size: 892 21 9 18 198 124 290 5889
Fragments arranged by size:
5889 892 290 198 124 21 18 9
BseYI C'CCAG_C
Cuts at: 2126 2301 2324 2347 2529 2810 2982 3883 4669
Size: 175 23 23 182 281 172 901 786
Cuts at: 4669 6644 7140 2126
Size: 1975 496 2427
Fragments arranged by size:
2427 1975 901 786 496 281 182 175
172 23 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2385 2398 2765 3227 3598 4350 1163
Size: 573 649 13 367 462 371 752 4254
Fragments arranged by size:
4254 752 649 573 462 371 367 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2866 4891 5652 6680 1540
Size: 275 186 865 2025 761 1028 2301
Fragments arranged by size:
2301 2025 1028 865 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3050 3386 3645 4589 5502 5587 6658 3050
Size: 336 259 944 913 85 1071 3833
Fragments arranged by size:
3833 1071 944 913 336 259 85
BsmI GAATG_Cn'
Cuts at: 2683 2827 3966 4952 5045 2683
Size: 144 1139 986 93 5079
Fragments arranged by size:
5079 1139 986 144 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2994 4639 2994
Size: 1645 5796
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2301 2321 2347 2440 2501 2703
Size: 399 337 174 20 26 93 61 202
Cuts at: 2703 2796 3050 3235 3386 3645 3768 3945 4103
Size: 93 254 185 151 259 123 177 158
Cuts at: 4103 4184 4378 4589 4760 5502 5587 6658 1391
Size: 81 194 211 171 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 337 259 254 211
202 194 185 177 174 171 158 151
123 93 93 85 81 61 26 20
BspEI T'CCGG_A
Cuts at: 7104 7104
Size: 7441
BspHI T'CATG_A
Cuts at: 4557 5330 7060 4557
Size: 773 1730 4938
Fragments arranged by size:
4938 1730 773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3115 3660 4796 844
Size: 2271 545 1136 3489
Fragments arranged by size:
3489 2271 1136 545
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 3436 5328 1394
Size: 429 236 1377 1892 3507
Fragments arranged by size:
3507 1892 1377 429 236
BsrDI GCAATG_nn'
Cuts at: 65 1248 2664 3984 4141 5936 6110 65
Size: 1183 1416 1320 157 1795 174 1396
Fragments arranged by size:
1795 1416 1396 1320 1183 174 157
BsrFI r'CCGG_y
Cuts at: 1700 1810 2508 4188 4674 6085 6272 1700
Size: 110 698 1680 486 1411 187 2869
Fragments arranged by size:
2869 1680 1411 698 486 187 110
BsrGI T'GTAC_A
Cuts at: 96 3309 96
Size: 3213 4228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7441
BssSI C'ACGA_G
Cuts at: 5501 6513 5501
Size: 1012 6429
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3561 3621 4131 4666 794
Size: 2767 60 510 535 3569
Fragments arranged by size:
3569 2767 535 510 60
BstBI TT'CG_AA
Cuts at: 6245 6245
Size: 7441
BstEII G'GTnAC_C
Cuts at: 1184 4391 1184
Size: 3207 4234
BstXI CCAn_nnnn'nTGG
Cuts at: 2491 3199 3254 4018 2491
Size: 708 55 764 5914
Fragments arranged by size:
5914 764 708 55
BstYI r'GATC_y
Cuts at: 1972 4771 5138 5523 5540 6992 7078 7090 7436
Size: 2799 367 385 17 1452 86 12 346
Cuts at: 7436 1972
Size: 1977
Fragments arranged by size:
2799 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3261 3333 3261
Size: 72 7369
BtgI C'CryG_G
Cuts at: 513 1065 2133 2235 2796 3198 4284 513
Size: 552 1068 102 561 402 1086 3670
Fragments arranged by size:
3670 1086 1068 561 552 402 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2853 3189 487
Size: 683 56 1627 336 4739
Fragments arranged by size:
4739 1627 683 336 56
BtsI GCAGTG_nn'
Cuts at: 2403 3064 3186 4957 5751 5771 2403
Size: 661 122 1771 794 20 4073
Fragments arranged by size:
4073 1771 794 661 122 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7441
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2379 2414 529
Size: 35 1815 35 5556
Fragments arranged by size:
5556 1815 35 35
DraI TTT'AAA
Cuts at: 4745 4745
Size: 7441
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6448 809
Size: 507 5132 1802
Fragments arranged by size:
5132 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2863 2921 3129
Size: 54 1425 50 275 18 1033 58 208
Cuts at: 3129 4186 4281 4888 5777 7406 8
Size: 1057 95 607 889 1629 43
Fragments arranged by size:
1629 1425 1057 1033 889 607 275 208
95 58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2863 4888 1537
Size: 275 1051 2025 4090
Fragments arranged by size:
4090 2025 1051 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3279 3374 3533 3887 4199 4712 5371 1353
Size: 1926 95 159 354 312 513 659 3423
Fragments arranged by size:
3423 1926 659 513 354 312 159 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4688 6028 6402 6548 387
Size: 1064 138 3099 1340 374 146 1280
Fragments arranged by size:
3099 1340 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3048 3048
Size: 7441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2403 2991 3290 3590 3803 3974
Size: 393 373 398 588 299 300 213 171
Cuts at: 3974 4463 4481 4739 5504 6101 6888 7156 1239
Size: 489 18 258 765 597 787 268 1524
Fragments arranged by size:
1524 787 765 597 588 489 398 393
373 300 299 268 258 213 171 18
EcoNI CCTnn'n_nnAGG
Cuts at: 4175 4175
Size: 7441
EcoO109I rG'GnC_Cy
Cuts at: 2206 2343 2344 2584 2699 3118 3232 3265 4099
Size: 137 1 240 115 419 114 33 834
Cuts at: 4099 4168 4374 2206
Size: 69 206 5273
Fragments arranged by size:
5273 834 419 240 206 137 115 114
69 33 1
EcoRI G'AATT_C
Cuts at: 4750 5128 4750
Size: 378 7063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3580 3580
Size: 7441
FspI TGC'GCA
Cuts at: 5947 5947
Size: 7441
HaeII r_GCGC'y
Cuts at: 1678 2119 2256 6588 1678
Size: 441 137 4332 2531
Fragments arranged by size:
4332 2531 441 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2750 2782 3794 3826 6082
Size: 32 135 32 1178 32 1012 32 2256
Cuts at: 6082 6114 6156 6188 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2256 1178 1012 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4153 4785 5031 669
Size: 988 281 2215 632 246 3079
Fragments arranged by size:
3079 2215 988 632 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7441
HpaI GTT'AAC
Cuts at: 5031 5031
Size: 7441
KasI G'GCGC_C
Cuts at: 2252 2252
Size: 7441
KpnI G_GTAC'C
Cuts at: 3777 4766 3777
Size: 989 6452
MfeI C'AATT_G
Cuts at: 5040 5040
Size: 7441
MluI A'CGCG_T
Cuts at: 7317 7317
Size: 7441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3514 4353 4662 6555 6739 1305
Size: 2209 839 309 1893 184 2007
Fragments arranged by size:
2209 2007 1893 839 309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2923 3131 4283 10
Size: 54 1425 343 1091 208 1152 3168
Fragments arranged by size:
3168 1425 1152 1091 343 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 3317 3323 3908 3971 4016 4592
Size: 648 1323 828 6 585 63 45 576
Cuts at: 4592 5399 5758 5917 518
Size: 807 359 159 2042
Fragments arranged by size:
2042 1323 828 807 648 585 576 359
159 63 45 6
MspA1I CmG'CkG
Cuts at: 1606 2025 2369 2551 2969 2982 3043 3341 3389
Size: 419 344 182 418 13 61 298 48
Cuts at: 3389 3887 4364 4717 4857 5534 6682 6927 1606
Size: 498 477 353 140 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 498 477 419 418 353
344 298 245 182 140 61 48 13
NaeI GCC'GGC
Cuts at: 2510 4190 2510
Size: 1680 5761
NarI GG'CG_CC
Cuts at: 2253 2253
Size: 7441
NcoI C'CATG_G
Cuts at: 513 1065 2235 4284 513
Size: 552 1170 2049 3670
Fragments arranged by size:
3670 2049 1170 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7441
NgoMIV G'CCGG_C
Cuts at: 2508 4188 2508
Size: 1680 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7441
NotI GC'GGCC_GC
Cuts at: 4888 4888
Size: 7441
NruI TCG'CGA
Cuts at: 4315 4315
Size: 7441
NspI r_CATG'y
Cuts at: 1132 1229 3322 3760 4799 6344 1132
Size: 97 2093 438 1039 1545 2229
Fragments arranged by size:
2229 2093 1545 1039 438 97
PasI CC'CwG_GG
Cuts at: 1348 3235 1348
Size: 1887 5554
PciI A'CATG_T
Cuts at: 3318 3756 6340 3318
Size: 438 2584 4419
Fragments arranged by size:
4419 2584 438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7441
PfoI T'CCnGG_A
Cuts at: 4089 4089
Size: 7441
PmeI GTTT'AAAC
Cuts at: 4745 4745
Size: 7441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3510 3542 5510 5542 7023 7055 3510
Size: 32 1968 32 1481 32 3896
Fragments arranged by size:
3896 1968 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2584 3118 2206
Size: 378 534 6529
Fragments arranged by size:
6529 534 378
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7441
PsiI TTA'TAA
Cuts at: 5011 5011
Size: 7441
PspOMI G'GGCC_C
Cuts at: 2343 2699 3231 4099 4374 2343
Size: 356 532 868 275 5410
Fragments arranged by size:
5410 868 532 356 275
PstI C_TGCA'G
Cuts at: 830 2446 3126 3671 4452 4793 830
Size: 1616 680 545 781 341 3478
Fragments arranged by size:
3478 1616 781 680 545 341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7441
PvuII CAG'CTG
Cuts at: 2369 2969 3341 3887 4364 2369
Size: 600 372 546 477 5446
Fragments arranged by size:
5446 600 546 477 372
SacI G_AGCT'C
Cuts at: 3050 3050
Size: 7441
SalI G'TCGA_C
Cuts at: 1936 4783 1936
Size: 2847 4594
SanDI GG'GwC_CC
Cuts at: 2584 2584
Size: 7441
SapI GCTCTTCn'nnn_
Cuts at: 3374 4199 3374
Size: 825 6616
SbfI CC_TGCA'GG
Cuts at: 3126 4793 3126
Size: 1667 5774
ScaI AGT'ACT
Cuts at: 5689 7401 5689
Size: 1712 5729
SfcI C'TryA_G
Cuts at: 826 1045 2442 3122 3667 3936 4448 4789 5924
Size: 219 1397 680 545 269 512 341 1135
Cuts at: 5924 6605 6796 826
Size: 681 191 1471
Fragments arranged by size:
1471 1397 1135 681 680 545 512 341
269 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3264 3264
Size: 7441
SfoI GGC'GCC
Cuts at: 2254 2254
Size: 7441
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7441
SmaI CCC'GGG
Cuts at: 1872 2032 2504 2704 4768 1872
Size: 160 472 200 2064 4545
Fragments arranged by size:
4545 2064 472 200 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2389 4322 5545 6446 6708 820
Size: 197 926 446 1933 1223 901 262 1553
Fragments arranged by size:
1933 1553 1223 926 901 446 262 197
SnaBI TAC'GTA
Cuts at: 493 5232 493
Size: 4739 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7441
SphI G_CATG'C
Cuts at: 1132 4799 1132
Size: 3667 3774
SspI AAT'ATT
Cuts at: 5 52 5365 5
Size: 47 5313 2081
Fragments arranged by size:
5313 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7441
StyI C'CwwG_G
Cuts at: 513 1065 2235 2421 2658 3192 3399 4233 4284
Size: 552 1170 186 237 534 207 834 51
Cuts at: 4284 4880 513
Size: 596 3074
Fragments arranged by size:
3074 1170 834 596 552 534 237 207
186 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2085 3313 5468 5485 5638 5823 6268
Size: 1255 505 1228 2155 17 153 185 445
Cuts at: 6268 325
Size: 1498
Fragments arranged by size:
2155 1498 1255 1228 505 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3309 5687 7399
Size: 274 80 33 51 1443 1332 2378 1712
Cuts at: 7399 96
Size: 138
Fragments arranged by size:
2378 1712 1443 1332 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2876 2908 7401 7433 2876
Size: 32 4493 32 2884
Fragments arranged by size:
4493 2884 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7441
XbaI T'CTAG_A
Cuts at: 4777 4777
Size: 7441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2278
Size: 7441
XhoI C'TCGA_G
Cuts at: 1943 4322 1943
Size: 2379 5062
XmaI C'CCGG_G
Cuts at: 1870 2030 2502 2702 4766 1870
Size: 160 472 200 2064 4545
Fragments arranged by size:
4545 2064 472 200 160
XmnI GAAnn'nnTTC
Cuts at: 3152 5570 3152
Size: 2418 5023
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4063 4633 5249 276
Size: 53 83 186 936 2529 570 616 2468
Fragments arranged by size:
2529 2468 936 616 570 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FseI FspAI NsiI PacI PmlI
PspXI PsrI RsrII SacII SexAI SrfI SwaI