Restriction map for
pFN21AB8467
(Circular) (Six-base) MAPSORT of: pFN21AB8467.seq Check: 6344 from: 1 to: 6052
pFN21AB8467 6052 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3036 3395 1937
Size: 1099 359 4594
Fragments arranged by size:
4594 1099 359
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4115 5499 5767 1632
Size: 2483 1384 268 1917
Fragments arranged by size:
2483 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 4951 5928 1203
Size: 3748 977 1327
Fragments arranged by size:
3748 1327 977
AgeI A'CCGG_T
Cuts at: 4883 4883
Size: 6052
AhdI GACnn_n'nnGTC
Cuts at: 4781 4781
Size: 6052
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6052
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 2076
Size: 32 6020
AlwNI CAG_nnn'CTG
Cuts at: 2886 3795 5367 2886
Size: 909 1572 3571
Fragments arranged by size:
3571 1572 909
ApaI G_GGCC'C
Cuts at: 2746 3172 2746
Size: 426 5626
ApaLI G'TGCA_C
Cuts at: 4109 5265 4109
Size: 1156 4896
ApoI r'AATT_y
Cuts at: 1493 3254 3347 3361 3587 3739 5848 1493
Size: 1761 93 14 226 152 2109 1697
Fragments arranged by size:
2109 1761 1697 226 152 93 14
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 2316 2923 3095 3377 1870
Size: 73 250 123 607 172 282 4545
Fragments arranged by size:
4545 607 282 250 172 123 73
BamHI G'GATC_C
Cuts at: 3382 3749 3382
Size: 367 5685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 2974 3373 4828 618
Size: 325 217 627 995 192 399 1455 1842
Fragments arranged by size:
1842 1455 995 627 399 325 217 192
BanII G_rGCy'C
Cuts at: 1391 2073 2556 2746 2898 3172 3371 1391
Size: 682 483 190 152 274 199 4072
Fragments arranged by size:
4072 682 483 274 199 190 152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3279 928
Size: 971 1380 3701
Fragments arranged by size:
3701 1380 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4243 4277 1903
Size: 34 50 34 2222 34 3678
Fragments arranged by size:
3678 2222 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 2280 1548
Size: 732 5320
BfrBI ATG'CAT
Cuts at: 2016 2016
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2403 3209 4663
Size: 107 122 71 631 605 731 806 1454
Cuts at: 4663 136
Size: 1525
Fragments arranged by size:
1525 1454 806 731 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2209 6047 2209
Size: 3838 2214
BlpI GC'TnA_GC
Cuts at: 3137 3469 3137
Size: 332 5720
Bme1580I G_kGCm'C
Cuts at: 1790 2193 2438 2746 2787 2979 3172 4113 5269
Size: 403 245 308 41 192 193 941 1156
Cuts at: 5269 1790
Size: 2573
Fragments arranged by size:
2573 1156 941 403 308 245 193 192
41
BmgBI CAC'GTC
Cuts at: 1318 2971 1318
Size: 1653 4399
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3783 4741 442
Size: 416 53 83 399 24 2366 958 1753
Fragments arranged by size:
2366 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2127 1056
Size: 1071 4981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2525 3308 4712 1239
Size: 995 291 783 1404 2579
Fragments arranged by size:
2579 1404 995 783 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2103 4177 5042 5340 2103
Size: 2074 865 298 2815
Fragments arranged by size:
2815 2074 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2539 3096 4715 882
Size: 617 1040 557 1619 2219
Fragments arranged by size:
2219 1619 1040 617 557
BsaAI yAC'GTr
Cuts at: 493 1204 2029 3843 493
Size: 711 825 1814 2702
Fragments arranged by size:
2702 1814 825 711
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3859 4241 4876 275
Size: 53 83 186 936 2326 382 635 1451
Fragments arranged by size:
2326 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4485 4883 5157 5304 5715 4485
Size: 398 274 147 411 4822
Fragments arranged by size:
4822 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5827
Fragments arranged by size:
5827 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6052
BseYI C'CCAG_C
Cuts at: 2064 2521 5255 5751 2064
Size: 457 2734 496 2365
Fragments arranged by size:
2734 2365 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3137 1163
Size: 573 1401 4078
Fragments arranged by size:
4078 1401 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3087 3502 4263 5291 1540
Size: 275 186 1086 415 761 1028 2301
Fragments arranged by size:
2301 1086 1028 761 415 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 4113 4198 5269 2073
Size: 2040 85 1071 2856
Fragments arranged by size:
2856 2040 1071 85
BsmI GAATG_Cn'
Cuts at: 2018 3563 3656 2018
Size: 1545 93 4414
Fragments arranged by size:
4414 1545 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2193 2438 2556 2746 2787 2898
Size: 399 283 120 245 118 190 41 111
Cuts at: 2898 2979 3172 3371 4113 4198 5269 1391
Size: 81 193 199 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 245 199 193
190 120 118 111 85 81 41
BspEI T'CCGG_A
Cuts at: 5715 5715
Size: 6052
BspHI T'CATG_A
Cuts at: 2202 3941 5671 2202
Size: 1739 1730 2583
Fragments arranged by size:
2583 1739 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3407 844
Size: 2563 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 1394
Size: 429 2116 3507
Fragments arranged by size:
3507 2116 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2062 2182 3153 4547 4721 65
Size: 1183 814 120 971 1394 174 1396
Fragments arranged by size:
1396 1394 1183 971 814 174 120
BsrFI r'CCGG_y
Cuts at: 1700 1810 4696 4883 1700
Size: 110 2886 187 2869
Fragments arranged by size:
2886 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 4112 5124 4112
Size: 1012 5040
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2061 794
Size: 1267 4785
BstBI TT'CG_AA
Cuts at: 4856 4856
Size: 6052
BstEII G'GTnAC_C
Cuts at: 1184 2812 1184
Size: 1628 4424
BstXI CCAn_nnnn'nTGG
Cuts at: 2426 2426
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 2209 3382 3749 4134 4151 5603 5689 5701
Size: 237 1173 367 385 17 1452 86 12
Cuts at: 5701 6047 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1173 385 367 346 237 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3087 3147 513
Size: 552 2022 60 3418
Fragments arranged by size:
3418 2022 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5313
Fragments arranged by size:
5313 683 56
BtsI GCAGTG_nn'
Cuts at: 2550 2718 3568 4362 4382 2550
Size: 168 850 794 20 4220
Fragments arranged by size:
4220 850 794 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3136 3171 529
Size: 35 2572 35 3410
Fragments arranged by size:
3410 2572 35 35
DraI TTT'AAA
Cuts at: 3356 3356
Size: 6052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5059 809
Size: 507 3743 1802
Fragments arranged by size:
3743 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2868 3108 3150
Size: 54 1425 50 275 18 1038 240 42
Cuts at: 3150 3499 4388 6017 8
Size: 349 889 1629 43
Fragments arranged by size:
1629 1425 1038 889 349 275 240 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 2267 3982 1353
Size: 725 189 1715 3423
Fragments arranged by size:
3423 1715 725 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4639 5013 5159 387
Size: 1064 138 3050 374 146 1280
Fragments arranged by size:
3050 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2071
Size: 6052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2525 3308 4115 4712 5499 5767
Size: 393 602 291 783 807 597 787 268
Cuts at: 5767 1239
Size: 1524
Fragments arranged by size:
1524 807 787 783 602 597 393 291
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3110 3290 3110
Size: 180 5872
EcoO109I rG'GnC_Cy
Cuts at: 2742 2743 2742
Size: 1 6051
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3229 3229
Size: 6052
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 2668 5199 1678
Size: 990 2531 2531
Fragments arranged by size:
2531 2531 990
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2375 2407 4693
Size: 32 135 32 557 32 214 32 2286
Cuts at: 4693 4725 4767 4799 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2286 557 214 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3396 3642 669
Size: 988 281 1458 246 3079
Fragments arranged by size:
3079 1458 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5166 5350 1305
Size: 3861 184 2007
Fragments arranged by size:
3861 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3110 10
Size: 54 1425 343 1278 2952
Fragments arranged by size:
2952 1425 1278 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3225 4010 4369 4528 518
Size: 648 2059 785 359 159 2042
Fragments arranged by size:
2059 2042 785 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2278 2809 3121 3468 4145 5293 5538 1606
Size: 672 531 312 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 531 347 312 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5500
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 2123 1052
Size: 1071 4981
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NsiI A_TGCA'T
Cuts at: 2018 2018
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 2189 2956 3136 3410 4955 1132
Size: 97 960 767 180 274 1545 2229
Fragments arranged by size:
2229 1545 960 767 274 180 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6052
PciI A'CATG_T
Cuts at: 4951 4951
Size: 6052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6052
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PmlI CAC'GTG
Cuts at: 2029 2029
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 5634 5666 4121
Size: 32 1481 32 4507
Fragments arranged by size:
4507 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3042 3089 1613
Size: 1429 47 4576
Fragments arranged by size:
4576 1429 47
PsiI TTA'TAA
Cuts at: 3622 3622
Size: 6052
PspOMI G'GGCC_C
Cuts at: 2742 3168 2742
Size: 426 5626
PstI C_TGCA'G
Cuts at: 830 2403 2545 3404 830
Size: 1573 142 859 3478
Fragments arranged by size:
3478 1573 859 142
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 2278 2278
Size: 6052
SacI G_AGCT'C
Cuts at: 2073 2073
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SapI GCTCTTCn'nnn_
Cuts at: 2078 2267 2078
Size: 189 5863
SbfI CC_TGCA'GG
Cuts at: 2403 3404 2403
Size: 1001 5051
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SexAI A'CCwGG_T
Cuts at: 2352 2778 2352
Size: 426 5626
SfcI C'TryA_G
Cuts at: 826 1045 2223 2399 2541 3400 4535 5216 5407
Size: 219 1178 176 142 859 1135 681 191
Cuts at: 5407 826
Size: 1471
Fragments arranged by size:
1471 1178 1135 859 681 219 191 176
142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 2195 2318 3097 3379 1872
Size: 323 123 779 282 4545
Fragments arranged by size:
4545 779 323 282 123
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2118 4156 5057 5319 820
Size: 197 926 175 2038 901 262 1553
Fragments arranged by size:
2038 1553 926 901 262 197 175
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 2956 3136 3410 1132
Size: 1824 180 274 3774
Fragments arranged by size:
3774 1824 274 180
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 3164 1517
Size: 1647 4405
StyI C'CwwG_G
Cuts at: 513 1065 2979 3111 3491 513
Size: 552 1914 132 380 3074
Fragments arranged by size:
3074 1914 552 380 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2591 4079 4096 4249 4434 4879 325
Size: 1255 1011 1488 17 153 185 445 1498
Fragments arranged by size:
1498 1488 1255 1011 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2937 4298 6010
Size: 274 80 33 51 1443 960 1361 1712
Cuts at: 6010 96
Size: 138
Fragments arranged by size:
1712 1443 1361 960 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6012 6044 6012
Size: 32 6020
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2426 2905 2426
Size: 479 5573
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6052
XmaI C'CCGG_G
Cuts at: 1870 2193 2316 3095 3377 1870
Size: 323 123 779 282 4545
Fragments arranged by size:
4545 779 323 282 123
XmnI GAAnn'nnTTC
Cuts at: 4181 4181
Size: 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI