Restriction map for
pFN21AB8464
(Circular) (Six-base) MAPSORT of: pFN21AB8464.seq Check: 4146 from: 1 to: 7084
pFN21AB8464 7084 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4168 4168
Size: 7084
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4894 278
Size: 53 83 186 936 3358 2468
Fragments arranged by size:
3358 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4427 1937
Size: 2490 4594
Acc65I G'GTAC_C
Cuts at: 4405 4405
Size: 7084
AclI AA'CG_TT
Cuts at: 1563 5211 5584 1563
Size: 3648 373 3063
Fragments arranged by size:
3648 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3672 3711 4079 5147 6531 6799 1632
Size: 2040 39 368 1068 1384 268 1917
Fragments arranged by size:
2040 1917 1384 1068 368 268 39
AfeI AGC'GCT
Cuts at: 2942 2942
Size: 7084
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6887
AflIII A'CryG_T
Cuts at: 1203 5983 6960 1203
Size: 4780 977 1327
Fragments arranged by size:
4780 1327 977
AgeI A'CCGG_T
Cuts at: 5915 5915
Size: 7084
AhdI GACnn_n'nnGTC
Cuts at: 5813 5813
Size: 7084
AleI CACnn'nnGTG
Cuts at: 1166 3728 1166
Size: 2562 4522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7084
AlwNI CAG_nnn'CTG
Cuts at: 2386 2548 3218 4183 4827 6399 2386
Size: 162 670 965 644 1572 3071
Fragments arranged by size:
3071 1572 965 670 644 162
ApaI G_GGCC'C
Cuts at: 2923 2923
Size: 7084
ApaLI G'TGCA_C
Cuts at: 5141 6297 5141
Size: 1156 5928
ApoI r'AATT_y
Cuts at: 1493 2201 2756 4393 4619 4771 6880 1493
Size: 708 555 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 708 555 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5638 160
Size: 872 4606 1606
Fragments arranged by size:
4606 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7084
AvaI C'yCGr_G
Cuts at: 1870 1943 2712 4409 1870
Size: 73 769 1697 4545
Fragments arranged by size:
4545 1697 769 73
BamHI G'GATC_C
Cuts at: 2214 4414 4781 2214
Size: 2200 367 4517
Fragments arranged by size:
4517 2200 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2538 3610 3889 3979
Size: 325 217 627 242 509 1072 279 90
Cuts at: 3979 4135 4405 5860 618
Size: 156 270 1455 1842
Fragments arranged by size:
1842 1455 1072 627 509 325 279 270
242 217 156 90
BanII G_rGCy'C
Cuts at: 1391 2061 2923 4044 4403 1391
Size: 670 862 1121 359 4072
Fragments arranged by size:
4072 1121 862 670 359
BbeI G_GCGC'C
Cuts at: 2542 2542
Size: 7084
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4067 4180 928
Size: 971 2168 113 3832
Fragments arranged by size:
3832 2168 971 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3314 3348 5275 5309 1903
Size: 34 50 34 1293 34 1927 34 3678
Fragments arranged by size:
3678 1927 1293 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4976 6186 4976
Size: 1210 5874
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7084
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3522 5695
Size: 107 122 71 631 605 337 1513 2173
Cuts at: 5695 136
Size: 1525
Fragments arranged by size:
2173 1525 1513 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2655 7079 2655
Size: 4424 2660
BlpI GC'TnA_GC
Cuts at: 3414 4501 3414
Size: 1087 5997
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2052 2433 2923 3111 3735 3894 4140
Size: 244 18 381 490 188 624 159 246
Cuts at: 4140 5145 6301 1790
Size: 1005 1156 2573
Fragments arranged by size:
2573 1156 1005 624 490 381 246 244
188 159 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7084
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3261 4815 5773
Size: 416 53 83 399 24 1844 1554 958
Cuts at: 5773 442
Size: 1753
Fragments arranged by size:
1844 1753 1554 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7084
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4063 4063
Size: 7084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 3138 3195 3207 3341 3554 3741 3978
Size: 888 1011 57 12 134 213 187 237
Cuts at: 3978 5744 1239
Size: 1766 2579
Fragments arranged by size:
2579 1766 1011 888 237 213 187 134
57 12
Bpu10I CC'TnA_GC
Cuts at: 2322 2322
Size: 7084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 4343 5209 6074 6372 2675
Size: 1668 866 865 298 3387
Fragments arranged by size:
3387 1668 866 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2414 4219 5747 882
Size: 617 915 1805 1528 2219
Fragments arranged by size:
2219 1805 1528 915 617
BsaAI yAC'GTr
Cuts at: 493 1204 4875 493
Size: 711 3671 2702
Fragments arranged by size:
3671 2702 711
BsaBI GATnn'nnATC
Cuts at: 3183 3231 7078 3183
Size: 48 3847 3189
Fragments arranged by size:
3847 3189 48
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2539 4891 5273 5908
Size: 53 83 186 936 1006 2352 382 635
Cuts at: 5908 275
Size: 1451
Fragments arranged by size:
2352 1451 1006 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5517 5915 6189 6336 6747 5517
Size: 398 274 147 411 5854
Fragments arranged by size:
5854 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2972 3002 1178
Size: 30 165 30 1569 30 5260
Fragments arranged by size:
5260 1569 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 2433 2804 2924 3011 3038 3488 3491
Size: 944 303 371 120 87 27 450 3
Cuts at: 3491 4100 4103 1186
Size: 609 3 4167
Fragments arranged by size:
4167 944 609 450 371 303 120 87
27 3 3
BseYI C'CCAG_C
Cuts at: 2376 2553 2914 3036 3540 3644 3704 3988 6287
Size: 177 361 122 504 104 60 284 2299
Cuts at: 6287 6783 2376
Size: 496 2677
Fragments arranged by size:
2677 2299 504 496 361 284 177 122
104 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2042 2088 1163
Size: 573 306 46 6159
Fragments arranged by size:
6159 573 306 46
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 4534 5295 6323 1540
Size: 275 186 12 2521 761 1028 2301
Fragments arranged by size:
2521 2301 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 5145 5230 6301 5145
Size: 85 1071 5928
Fragments arranged by size:
5928 1071 85
BsmI GAATG_Cn'
Cuts at: 4595 4688 4595
Size: 93 6991
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2052 2061 2433 2923 3111 3735
Size: 399 244 18 9 372 490 188 624
Cuts at: 3735 3894 4044 4140 4403 5145 5230 6301 1391
Size: 159 150 96 263 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 624 490 399 372 263
244 188 159 150 96 85 18 9
BspEI T'CCGG_A
Cuts at: 6747 6747
Size: 7084
BspHI T'CATG_A
Cuts at: 4237 4973 6703 4237
Size: 736 1730 4618
Fragments arranged by size:
4618 1730 736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4168 4439 844
Size: 3324 271 3489
Fragments arranged by size:
3489 3324 271
BsrBI CCG'CTC
Cuts at: 1394 1823 2806 3418 4971 1394
Size: 429 983 612 1553 3507
Fragments arranged by size:
3507 1553 983 612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2460 5579 5753 65
Size: 1183 1212 3119 174 1396
Fragments arranged by size:
3119 1396 1212 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 5728 5915 1700
Size: 110 198 3720 187 2869
Fragments arranged by size:
3720 2869 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7084
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7084
BssSI C'ACGA_G
Cuts at: 5144 6156 5144
Size: 1012 6072
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2029 2386 3106 794
Size: 1235 357 720 4772
Fragments arranged by size:
4772 1235 720 357
BstBI TT'CG_AA
Cuts at: 5888 5888
Size: 7084
BstEII G'GTnAC_C
Cuts at: 1184 3502 1184
Size: 2318 4766
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 3607 4312 2182
Size: 1425 705 4954
Fragments arranged by size:
4954 1425 705
BstYI r'GATC_y
Cuts at: 1972 2214 2655 3261 3480 4414 4781 5166 5183
Size: 242 441 606 219 934 367 385 17
Cuts at: 5183 6635 6721 6733 7079 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 934 606 441 385 367 346
242 219 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2052 4029 513
Size: 552 987 1977 3568
Fragments arranged by size:
3568 1977 987 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3372 487
Size: 683 56 2146 4199
Fragments arranged by size:
4199 2146 683 56
BtsI GCAGTG_nn'
Cuts at: 4600 5394 5414 4600
Size: 794 20 6270
Fragments arranged by size:
6270 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7084
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7049
DraI TTT'AAA
Cuts at: 4388 4388
Size: 7084
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6091 809
Size: 507 4775 1802
Fragments arranged by size:
4775 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2184 2526
Size: 54 1425 50 275 18 180 174 342
Cuts at: 2526 3514 4329 4531 5420 7049 8
Size: 988 815 202 889 1629 43
Fragments arranged by size:
1629 1425 988 889 815 342 275 202
180 174 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 4531 1537
Size: 275 198 2521 4090
Fragments arranged by size:
4090 2521 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2621 2781 5014 1353
Size: 1268 160 2233 3423
Fragments arranged by size:
3423 2233 1268 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2323 2588 3035 5671 6045 6191
Size: 1064 138 734 265 447 2636 374 146
Cuts at: 6191 387
Size: 1280
Fragments arranged by size:
2636 1280 1064 734 447 374 265 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 3138 3195 3207 3341 3554 3672
Size: 393 495 1011 57 12 134 213 118
Cuts at: 3672 3711 3741 3978 4079 5147 5744 6531 6799
Size: 39 30 237 101 1068 597 787 268
Cuts at: 6799 1239
Size: 1524
Fragments arranged by size:
1524 1068 1011 787 597 495 393 268
237 213 134 118 101 57 39 30
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2933 2933
Size: 7084
EcoO109I rG'GnC_Cy
Cuts at: 2919 2919
Size: 7084
EcoRI G'AATT_C
Cuts at: 4393 4771 4393
Size: 378 6706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 7084
FspI TGC'GCA
Cuts at: 3293 4100 5590 3293
Size: 807 1490 4787
Fragments arranged by size:
4787 1490 807
FspAI rTGC'GCAy
Cuts at: 3293 3293
Size: 7084
HaeII r_GCGC'y
Cuts at: 1678 2542 2944 6231 1678
Size: 864 402 3287 2531
Fragments arranged by size:
3287 2531 864 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 2972 3004 3503
Size: 32 135 32 887 32 481 32 499
Cuts at: 3503 3535 4199 4231 4292 4324 5725 5757 5799
Size: 32 664 32 61 32 1401 32 42
Cuts at: 5799 5831 1373
Size: 32 2626
Fragments arranged by size:
2626 1401 887 664 499 481 135 61
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 4428 4674 669
Size: 988 281 226 2264 246 3079
Fragments arranged by size:
3079 2264 988 281 246 226
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7084
HpaI GTT'AAC
Cuts at: 4674 4674
Size: 7084
KasI G'GCGC_C
Cuts at: 2538 2538
Size: 7084
KpnI G_GTAC'C
Cuts at: 4409 4409
Size: 7084
MfeI C'AATT_G
Cuts at: 4683 4683
Size: 7084
MluI A'CGCG_T
Cuts at: 6960 6960
Size: 7084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2242 2890 4329 6198 6382 1305
Size: 937 648 1439 1869 184 2007
Fragments arranged by size:
2007 1869 1439 937 648 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 2528 3516 4331 10
Size: 54 1425 343 354 342 988 815 2763
Fragments arranged by size:
2763 1425 988 815 354 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3728 3944 4226 4250 5042 5401 5560
Size: 648 2562 216 282 24 792 359 159
Cuts at: 5560 518
Size: 2042
Fragments arranged by size:
2562 2042 792 648 359 282 216 159
24
MspA1I CmG'CkG
Cuts at: 1606 2386 2557 3040 3328 4500 5177 6325 6570
Size: 780 171 483 288 1172 677 1148 245
Cuts at: 6570 1606
Size: 2120
Fragments arranged by size:
2120 1172 1148 780 677 483 288 245
171
NaeI GCC'GGC
Cuts at: 2010 2010
Size: 7084
NarI GG'CG_CC
Cuts at: 2539 2539
Size: 7084
NcoI C'CATG_G
Cuts at: 513 1065 4029 513
Size: 552 2964 3568
Fragments arranged by size:
3568 2964 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7084
NgoMIV G'CCGG_C
Cuts at: 2008 2008
Size: 7084
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7084
NotI GC'GGCC_GC
Cuts at: 4531 4531
Size: 7084
NspI r_CATG'y
Cuts at: 1132 1229 3292 4147 4255 4442 5987 1132
Size: 97 2063 855 108 187 1545 2229
Fragments arranged by size:
2229 2063 1545 855 187 108 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7084
PciI A'CATG_T
Cuts at: 5983 5983
Size: 7084
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7084
PfoI T'CCnGG_A
Cuts at: 2760 3570 2760
Size: 810 6274
PmeI GTTT'AAAC
Cuts at: 4388 4388
Size: 7084
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2441 2473 4076 4108 5153 5185 6666 6698 2441
Size: 32 1603 32 1045 32 1481 32 2827
Fragments arranged by size:
2827 1603 1481 1045 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7084
PsiI TTA'TAA
Cuts at: 4654 4654
Size: 7084
PspOMI G'GGCC_C
Cuts at: 2919 2919
Size: 7084
PstI C_TGCA'G
Cuts at: 830 4261 4436 830
Size: 3431 175 3478
Fragments arranged by size:
3478 3431 175
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7084
PvuII CAG'CTG
Cuts at: 2386 2557 3040 2386
Size: 171 483 6430
Fragments arranged by size:
6430 483 171
SalI G'TCGA_C
Cuts at: 1936 4426 1936
Size: 2490 4594
SbfI CC_TGCA'GG
Cuts at: 4261 4436 4261
Size: 175 6909
ScaI AGT'ACT
Cuts at: 5332 7044 5332
Size: 1712 5372
SexAI A'CCwGG_T
Cuts at: 3376 4209 3376
Size: 833 6251
SfcI C'TryA_G
Cuts at: 826 1045 3768 4257 4274 4432 5567 6248 6439
Size: 219 2723 489 17 158 1135 681 191
Cuts at: 6439 826
Size: 1471
Fragments arranged by size:
2723 1471 1135 681 489 219 191 158
17
SfoI GGC'GCC
Cuts at: 2540 2540
Size: 7084
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7084
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7084
SmaI CCC'GGG
Cuts at: 1872 4411 1872
Size: 2539 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2690 4358 5188 6089 6351 820
Size: 197 926 747 1668 830 901 262 1553
Fragments arranged by size:
1668 1553 926 901 830 747 262 197
SnaBI TAC'GTA
Cuts at: 493 4875 493
Size: 4382 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7084
SphI G_CATG'C
Cuts at: 1132 4255 4442 1132
Size: 3123 187 3774
Fragments arranged by size:
3774 3123 187
SspI AAT'ATT
Cuts at: 5 52 2158 5008 5
Size: 47 2106 2850 2081
Fragments arranged by size:
2850 2106 2081 47
StuI AGG'CCT
Cuts at: 1517 3606 1517
Size: 2089 4995
StyI C'CwwG_G
Cuts at: 513 1065 2346 4029 4523 513
Size: 552 1281 1683 494 3074
Fragments arranged by size:
3074 1683 1281 552 494
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 2683 3068 3142 3733 3757 5111
Size: 1255 810 293 385 74 591 24 1354
Cuts at: 5111 5128 5281 5466 5911 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1354 1255 810 591 445 385 293
185 153 74 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2646 3058 5330
Size: 274 80 33 51 1443 669 412 2272
Cuts at: 5330 7042 96
Size: 1712 138
Fragments arranged by size:
2272 1712 1443 669 412 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2717 2749 7044 7076 2717
Size: 32 4295 32 2725
Fragments arranged by size:
4295 2725 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3161 4211 1316
Size: 1845 1050 4189
Fragments arranged by size:
4189 1845 1050
XbaI T'CTAG_A
Cuts at: 4420 4420
Size: 7084
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3883 3883
Size: 7084
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7084
XmaI C'CCGG_G
Cuts at: 1870 4409 1870
Size: 2539 4545
XmnI GAAnn'nnTTC
Cuts at: 5213 5213
Size: 7084
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4892 276
Size: 53 83 186 936 3358 2468
Fragments arranged by size:
3358 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SrfI SwaI