Restriction map for
pFN21AB8460
(Circular) (Six-base) MAPSORT of: pFN21AB8460.seq Check: 8200 from: 1 to: 5461
pFN21AB8460 5461 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2804 1937
Size: 867 4594
Acc65I G'GTAC_C
Cuts at: 2782 2782
Size: 5461
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2614 3524 4908 5176 1632
Size: 982 910 1384 268 1917
Fragments arranged by size:
1917 1384 982 910 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 4190 4190
Size: 5461
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2603 1184
Size: 1419 4042
AlwNI CAG_nnn'CTG
Cuts at: 2473 2686 3204 4776 2473
Size: 213 518 1572 3158
Fragments arranged by size:
3158 1572 518 213
ApaLI G'TGCA_C
Cuts at: 3518 4674 3518
Size: 1156 4305
ApoI r'AATT_y
Cuts at: 1493 2770 2996 3148 5257 1493
Size: 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2237 2435 2757 2786 1870
Size: 73 294 198 322 29 4545
Fragments arranged by size:
4545 322 294 198 73 29
BamHI G'GATC_C
Cuts at: 2791 3158 2791
Size: 367 5094
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 4237 618
Size: 325 217 627 995 1455 1842
Fragments arranged by size:
1842 1455 995 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2442 2499 2651 2780 1391
Size: 1051 57 152 129 4072
Fragments arranged by size:
4072 1051 152 129 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2620 928
Size: 971 721 3769
Fragments arranged by size:
3769 971 721
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 1903
Size: 34 1715 34 3678
Fragments arranged by size:
3678 1715 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2198 3353 4563 2198
Size: 1155 1210 3096
Fragments arranged by size:
3096 1210 1155
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4072 136
Size: 107 122 71 631 605 2400 1525
Fragments arranged by size:
2400 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2143 5456 2143
Size: 3313 2148
BlpI GC'TnA_GC
Cuts at: 2493 2878 2493
Size: 385 5076
Bme1580I G_kGCm'C
Cuts at: 1790 3522 4678 1790
Size: 1732 1156 2573
Fragments arranged by size:
2573 1732 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2244 2321 3192
Size: 416 53 83 399 24 827 77 871
Cuts at: 3192 4150 442
Size: 958 1753
Fragments arranged by size:
1753 958 871 827 416 399 83 77
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2591 2701 4121 1239
Size: 875 477 110 1420 2579
Fragments arranged by size:
2579 1420 875 477 110
Bpu10I CC'TnA_GC
Cuts at: 2441 2441
Size: 5461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3586 4451 4749 3586
Size: 865 298 4298
Fragments arranged by size:
4298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2252 4124 882
Size: 617 753 1872 2219
Fragments arranged by size:
2219 1872 753 617
BsaAI yAC'GTr
Cuts at: 493 1204 2186 3252 493
Size: 711 982 1066 2702
Fragments arranged by size:
2702 1066 982 711
BsaBI GATnn'nnATC
Cuts at: 5455 5455
Size: 5461
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3268 3650 4285 275
Size: 53 83 186 936 1735 382 635 1451
Fragments arranged by size:
1735 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2655 3894 4292 4566 4713 5124 2655
Size: 1239 398 274 147 411 2992
Fragments arranged by size:
2992 1239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5236
Fragments arranged by size:
5236 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 1186
Size: 1084 4377
BseYI C'CCAG_C
Cuts at: 2272 2466 4664 5160 2272
Size: 194 2198 496 2573
Fragments arranged by size:
2573 2198 496 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 1163
Size: 573 573 4315
Fragments arranged by size:
4315 573 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2911 3672 4700 1540
Size: 275 186 910 761 1028 2301
Fragments arranged by size:
2301 1028 910 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 2223 3522 3607 4678 2118
Size: 105 1299 85 1071 2901
Fragments arranged by size:
2901 1299 1071 105 85
BsmI GAATG_Cn'
Cuts at: 2972 3065 2972
Size: 93 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2223 2442 2499 2651 2780 3522
Size: 399 328 105 219 57 152 129 742
Cuts at: 3522 3607 4678 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 219 152 129
105 85 57
BspEI T'CCGG_A
Cuts at: 5124 5124
Size: 5461
BspHI T'CATG_A
Cuts at: 2736 3350 5080 2736
Size: 614 1730 3117
Fragments arranged by size:
3117 1730 614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2571 2816 844
Size: 1727 245 3489
Fragments arranged by size:
3489 1727 245
BsrBI CCG'CTC
Cuts at: 1394 1823 3348 1394
Size: 429 1525 3507
Fragments arranged by size:
3507 1525 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3956 4130 65
Size: 1183 2708 174 1396
Fragments arranged by size:
2708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4105 4292 1700
Size: 110 2295 187 2869
Fragments arranged by size:
2869 2295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5461
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5461
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5461
BstBI TT'CG_AA
Cuts at: 4265 4265
Size: 5461
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5461
BstYI r'GATC_y
Cuts at: 1972 2143 2791 3158 3543 3560 5012 5098 5110
Size: 171 648 367 385 17 1452 86 12
Cuts at: 5110 5456 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 648 385 367 346 171 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2264 2687 2264
Size: 423 5038
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3970
Fragments arranged by size:
3970 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2585 487
Size: 683 56 1359 3363
Fragments arranged by size:
3363 1359 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 2977 3771 3791 2221
Size: 756 794 20 3891
Fragments arranged by size:
3891 794 756 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DraIII CAC_nnn'GTG
Cuts at: 2617 2617
Size: 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2334 2509
Size: 54 1425 50 275 18 292 212 175
Cuts at: 2509 2908 3797 5426 8
Size: 399 889 1629 43
Fragments arranged by size:
1629 1425 889 399 292 275 212 175
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2908 1537
Size: 275 1096 4090
Fragments arranged by size:
4090 1096 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3391 1353
Size: 2038 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4048 4422 4568 387
Size: 1064 138 2459 374 146 1280
Fragments arranged by size:
2459 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2591 2614 2701 3524 4121 4908
Size: 393 482 477 23 87 823 597 787
Cuts at: 4908 5176 1239
Size: 268 1524
Fragments arranged by size:
1524 823 787 597 482 477 393 268
87 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 2690 2357
Size: 333 5128
EcoO109I rG'GnC_Cy
Cuts at: 2241 2517 2683 2241
Size: 276 166 5019
Fragments arranged by size:
5019 276 166
EcoRI G'AATT_C
Cuts at: 2770 3148 2770
Size: 378 5083
FspI TGC'GCA
Cuts at: 2702 3967 2702
Size: 1265 4196
HaeII r_GCGC'y
Cuts at: 1678 2236 2353 4608 1678
Size: 558 117 2255 2531
Fragments arranged by size:
2531 2255 558 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4102 4134 4176 4208 1373
Size: 32 135 32 2530 32 42 32 2626
Fragments arranged by size:
2626 2530 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KpnI G_GTAC'C
Cuts at: 2786 2786
Size: 5461
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4575 4759 1305
Size: 3270 184 2007
Fragments arranged by size:
3270 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2124 10
Size: 54 1425 343 292 3347
Fragments arranged by size:
3347 1425 343 292 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3419 3778 3937 518
Size: 648 2253 359 159 2042
Fragments arranged by size:
2253 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2470 2752 2877 3554 4702 4947 1606
Size: 488 376 282 125 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 488 376 282 245 125
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3970
Fragments arranged by size:
3970 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2819 4364 1132
Size: 97 1590 1545 2229
Fragments arranged by size:
2229 1590 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5461
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 2371 2473 1108
Size: 1089 174 102 4096
Fragments arranged by size:
4096 1089 174 102
PfoI T'CCnGG_A
Cuts at: 2169 2717 2169
Size: 548 4913
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2438 2470 2438
Size: 32 5429
PstI C_TGCA'G
Cuts at: 830 2228 2295 2415 2582 2813 830
Size: 1398 67 120 167 231 3478
Fragments arranged by size:
3478 1398 231 167 120 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
PvuII CAG'CTG
Cuts at: 2094 2470 2752 2094
Size: 376 282 4803
Fragments arranged by size:
4803 376 282
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SbfI CC_TGCA'GG
Cuts at: 2813 2813
Size: 5461
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SfcI C'TryA_G
Cuts at: 826 1045 2085 2224 2291 2411 2578 2809 3944
Size: 219 1040 139 67 120 167 231 1135
Cuts at: 3944 4625 4816 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1040 681 231 219 191 167
139 120 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2239 2759 2788 1872
Size: 367 520 29 4545
Fragments arranged by size:
4545 520 367 29
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3565 4466 4728 820
Size: 197 926 1622 901 262 1553
Fragments arranged by size:
1622 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2186 3252 493
Size: 1693 1066 2702
Fragments arranged by size:
2702 1693 1066
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2819 1132
Size: 1687 3774
SspI AAT'ATT
Cuts at: 5 52 3385 5
Size: 47 3333 2081
Fragments arranged by size:
3333 2081 47
StuI AGG'CCT
Cuts at: 1517 2196 1517
Size: 679 4782
StyI C'CwwG_G
Cuts at: 513 1065 2004 2119 2191 2358 2900 513
Size: 552 939 115 72 167 542 3074
Fragments arranged by size:
3074 939 552 542 167 115 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3488 3505 3658 3843 4288 325
Size: 1255 1908 17 153 185 445 1498
Fragments arranged by size:
1908 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5419 96
Size: 274 80 33 51 1443 1730 1712 138
Fragments arranged by size:
1730 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5421 5453 5421
Size: 32 5429
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2167
Size: 5461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5461
XmaI C'CCGG_G
Cuts at: 1870 2237 2757 2786 1870
Size: 367 520 29 4545
Fragments arranged by size:
4545 520 367 29
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 5461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI