Restriction map for
pFN21AB8459
(Circular) (Six-base) MAPSORT of: pFN21AB8459.seq Check: 2882 from: 1 to: 6109
pFN21AB8459 6109 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3452 1937
Size: 1515 4594
Acc65I G'GTAC_C
Cuts at: 2200 3430 2200
Size: 1230 4879
AclI AA'CG_TT
Cuts at: 1563 2708 3225 3273 4236 4609 1563
Size: 1145 517 48 963 373 3063
Fragments arranged by size:
3063 1145 963 517 373 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 2754 4172 5556 5824 1632
Size: 456 666 1418 1384 268 1917
Fragments arranged by size:
1917 1418 1384 666 456 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 5008 5985 1203
Size: 3805 977 1327
Fragments arranged by size:
3805 1327 977
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3233 1184
Size: 2049 4060
AlwNI CAG_nnn'CTG
Cuts at: 3852 5424 3852
Size: 1572 4537
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 2623 3384 3418 3644 3796 5905 1493
Size: 1130 761 34 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 761 226 152 34
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 3434 1870
Size: 73 1491 4545
Fragments arranged by size:
4545 1491 73
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2200 3430 4885 618
Size: 325 217 627 413 1230 1455 1842
Fragments arranged by size:
1842 1455 1230 627 413 325 217
BanII G_rGCy'C
Cuts at: 1391 2855 3428 1391
Size: 1464 573 4072
Fragments arranged by size:
4072 1464 573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3179 928
Size: 971 1280 3858
Fragments arranged by size:
3858 1280 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3299 3333 4300 4334 1903
Size: 34 1362 34 967 34 3678
Fragments arranged by size:
3678 1362 967 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4720 136
Size: 107 122 71 631 605 3048 1525
Fragments arranged by size:
3048 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6104 6104
Size: 6109
BlpI GC'TnA_GC
Cuts at: 3526 3526
Size: 6109
Bme1580I G_kGCm'C
Cuts at: 1790 2296 4170 5326 1790
Size: 506 1874 1156 2573
Fragments arranged by size:
2573 1874 1156 506
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3840 4798 442
Size: 416 53 83 399 24 2423 958 1753
Fragments arranged by size:
2423 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2187 2229 2265 2887 3413 4769 1239
Size: 948 42 36 622 526 1356 2579
Fragments arranged by size:
2579 1356 948 622 526 42 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4234 5099 5397 4234
Size: 865 298 4946
Fragments arranged by size:
4946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4772 882
Size: 617 3273 2219
Fragments arranged by size:
3273 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2527 3900 493
Size: 711 1323 1373 2702
Fragments arranged by size:
2702 1373 1323 711
BsaBI GATnn'nnATC
Cuts at: 2155 6103 2155
Size: 3948 2161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3916 4298 4933 275
Size: 53 83 186 936 2383 382 635 1451
Fragments arranged by size:
2383 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4542 4940 5214 5361 5772 4542
Size: 398 274 147 411 4879
Fragments arranged by size:
4879 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2650 2680 1178
Size: 30 165 30 1247 30 4607
Fragments arranged by size:
4607 1247 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2184 2226 2416 2419 1186
Size: 998 42 190 3 4876
Fragments arranged by size:
4876 998 190 42 3
BseYI C'CCAG_C
Cuts at: 5312 5808 5312
Size: 496 5613
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5536
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3559 4320 5348 1540
Size: 275 186 1558 761 1028 2301
Fragments arranged by size:
2301 1558 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4170 4255 5326 4170
Size: 85 1071 4953
Fragments arranged by size:
4953 1071 85
BsmI GAATG_Cn'
Cuts at: 3005 3322 3620 3713 3005
Size: 317 298 93 5401
Fragments arranged by size:
5401 317 298 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2296 2855 3428 4170 4255 5326 1391
Size: 399 506 559 573 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 573 559 506 399 85
BspEI T'CCGG_A
Cuts at: 5772 5772
Size: 6109
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3464 844
Size: 2620 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4604 4778 65
Size: 1183 3356 174 1396
Fragments arranged by size:
3356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4753 4940 1700
Size: 110 2943 187 2869
Fragments arranged by size:
2943 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 4169 5181 4169
Size: 1012 5097
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6109
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 2326 3439 3806 4191 4208 5660 5746 5758
Size: 354 1113 367 385 17 1452 86 12
Cuts at: 5758 6104 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1113 385 367 354 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 513
Size: 552 939 84 4534
Fragments arranged by size:
4534 939 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5370
Fragments arranged by size:
5370 683 56
BtsI GCAGTG_nn'
Cuts at: 3625 4419 4439 3625
Size: 794 20 5295
Fragments arranged by size:
5295 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2359 2394 529
Size: 35 1795 35 4244
Fragments arranged by size:
4244 1795 35 35
DraI TTT'AAA
Cuts at: 2179 2188 2566 3124 3153 3413 2179
Size: 9 378 558 29 260 4875
Fragments arranged by size:
4875 558 378 260 29 9
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2091 2484
Size: 54 1425 50 275 18 177 84 393
Cuts at: 2484 3556 4445 6074 8
Size: 1072 889 1629 43
Fragments arranged by size:
1629 1425 1072 889 393 275 177 84
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2950 3015 4039 1353
Size: 711 886 65 1024 3423
Fragments arranged by size:
3423 1024 886 711 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4696 5070 5216 387
Size: 1064 138 3107 374 146 1280
Fragments arranged by size:
3107 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2187 2229 2265 2754 2887 3413
Size: 393 456 99 42 36 489 133 526
Cuts at: 3413 4172 4769 5556 5824 1239
Size: 759 597 787 268 1524
Fragments arranged by size:
1524 787 759 597 526 489 456 393
268 133 99 42 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2208 2259 2208
Size: 51 6058
EcoRI G'AATT_C
Cuts at: 2623 3418 3796 2623
Size: 795 378 4936
Fragments arranged by size:
4936 795 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 6109
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 2244 5256 1678
Size: 566 3012 2531
Fragments arranged by size:
3012 2531 566
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2750 2774 2782 2806 4750
Size: 32 135 32 1178 24 8 24 1944
Cuts at: 4750 4782 4824 4856 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1944 1178 135 42 32 32 32
32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2706 3453 3699 669
Size: 988 281 768 747 246 3079
Fragments arranged by size:
3079 988 768 747 281 246
HindIII A'AGCT_T
Cuts at: 748 2451 748
Size: 1703 4406
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 2204 3434 2204
Size: 1230 4879
MfeI C'AATT_G
Cuts at: 3303 3708 3303
Size: 405 5704
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2128 2440 5223 5407 1305
Size: 823 312 2783 184 2007
Fragments arranged by size:
2783 2007 823 312 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2093 10
Size: 54 1425 343 177 84 4026
Fragments arranged by size:
4026 1425 343 177 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4067 4426 4585 518
Size: 648 2901 359 159 2042
Fragments arranged by size:
2901 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2321 2654 3525 4202 5350 5595 1606
Size: 715 333 871 677 1148 245 2120
Fragments arranged by size:
2120 1148 871 715 677 333 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2088 513
Size: 552 939 84 4534
Fragments arranged by size:
4534 939 552 84
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 3467 5012 1132
Size: 97 2238 1545 2229
Fragments arranged by size:
2238 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6109
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PmlI CAC'GTG
Cuts at: 2527 2527
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PstI C_TGCA'G
Cuts at: 830 3461 830
Size: 2631 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 2654 2654
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 3159 3457 4592 5273 5464 826
Size: 219 2114 298 1135 681 191 1471
Fragments arranged by size:
2114 1471 1135 681 298 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4213 5114 5376 820
Size: 197 926 2270 901 262 1553
Fragments arranged by size:
2270 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 2582 4033 5
Size: 47 2530 1451 2081
Fragments arranged by size:
2530 2081 1451 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6109
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2331 2373 3548 513
Size: 552 939 84 243 42 1175 3074
Fragments arranged by size:
3074 1175 939 552 243 84 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3052 3253 4136 4153 4306 4491 4936
Size: 1255 1472 201 883 17 153 185 445
Cuts at: 4936 325
Size: 1498
Fragments arranged by size:
1498 1472 1255 883 445 201 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4355 6067 96
Size: 274 80 33 51 1443 2378 1712 138
Fragments arranged by size:
2378 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6069 6101 6069
Size: 32 6077
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6109
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2965 2965
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6109
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 4238 4238
Size: 6109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI