Restriction map for
pFN21AB8458
(Circular) (Six-base) MAPSORT of: pFN21AB8458.seq Check: 3939 from: 1 to: 5722
pFN21AB8458 5722 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2419 2419
Size: 5722
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2391 3532 278
Size: 53 83 186 936 855 1141 2468
Fragments arranged by size:
2468 1141 936 855 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2283 3006 3065 1937
Size: 346 723 59 4594
Fragments arranged by size:
4594 723 346 59
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3785 5169 5437 1632
Size: 2153 1384 268 1917
Fragments arranged by size:
2153 1917 1384 268
AfeI AGC'GCT
Cuts at: 2219 2916 2219
Size: 697 5025
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 4621 5598 1203
Size: 3418 977 1327
Fragments arranged by size:
3418 1327 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AlwNI CAG_nnn'CTG
Cuts at: 2023 3465 5037 2023
Size: 1442 1572 2708
Fragments arranged by size:
2708 1572 1442
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 2572 3031 3257 3409 5518 1493
Size: 1079 459 226 152 2109 1697
Fragments arranged by size:
2109 1697 1079 459 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 3047 1870
Size: 73 1104 4545
Fragments arranged by size:
4545 1104 73
BamHI G'GATC_C
Cuts at: 2153 3052 3419 2153
Size: 899 367 4456
Fragments arranged by size:
4456 899 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2431 3043 4498 618
Size: 325 217 627 644 612 1455 1842
Fragments arranged by size:
1842 1455 644 627 612 325 217
BanII G_rGCy'C
Cuts at: 1391 2823 3041 1391
Size: 1432 218 4072
Fragments arranged by size:
4072 1432 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2147 2748 928
Size: 971 248 601 3902
Fragments arranged by size:
3902 971 601 248
BbvCI CC'TCA_GC
Cuts at: 2874 2874
Size: 5722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3614 4824 2313
Size: 1301 1210 3211
Fragments arranged by size:
3211 1301 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 4333 136
Size: 107 122 71 631 605 400 2261 1525
Fragments arranged by size:
2261 1525 631 605 400 122 107 71
BglII A'GATC_T
Cuts at: 2782 5717 2782
Size: 2935 2787
BlpI GC'TnA_GC
Cuts at: 2354 3139 2354
Size: 785 4937
Bme1580I G_kGCm'C
Cuts at: 1790 2248 2436 2636 3783 4939 1790
Size: 458 188 200 1147 1156 2573
Fragments arranged by size:
2573 1156 1147 458 200 188
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3453 4411 442
Size: 416 53 83 399 24 2036 958 1753
Fragments arranged by size:
2036 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2585 2604 2648 2837 4382 1239
Size: 992 354 19 44 189 1545 2579
Fragments arranged by size:
2579 1545 992 354 189 44 19
Bpu10I CC'TnA_GC
Cuts at: 2874 2874
Size: 5722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3847 4712 5010 3847
Size: 865 298 4559
Fragments arranged by size:
4559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4385 882
Size: 617 2886 2219
Fragments arranged by size:
2886 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2287 3513 493
Size: 711 1083 1226 2702
Fragments arranged by size:
2702 1226 1083 711
BsaBI GATnn'nnATC
Cuts at: 5716 5716
Size: 5722
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2388 3529 3911 4546
Size: 53 83 186 936 855 1141 382 635
Cuts at: 4546 275
Size: 1451
Fragments arranged by size:
1451 1141 936 855 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2053 2391 4155 4553 4827 4974 5385 2053
Size: 338 1764 398 274 147 411 2390
Fragments arranged by size:
2390 1764 411 398 338 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5497
Fragments arranged by size:
5497 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2228 2591 2714 1186
Size: 922 120 363 123 4194
Fragments arranged by size:
4194 922 363 123 120
BseYI C'CCAG_C
Cuts at: 2016 2272 4925 5421 2016
Size: 256 2653 496 2317
Fragments arranged by size:
2653 2317 496 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2960 1163
Size: 573 1224 3925
Fragments arranged by size:
3925 1224 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3172 3933 4961 1540
Size: 275 186 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2823 3783 3868 4939 2823
Size: 960 85 1071 3606
Fragments arranged by size:
3606 1071 960 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2248 2436 2636 2823 3041 3783 3868
Size: 399 458 188 200 187 218 742 85
Cuts at: 3868 4939 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 218 200 188
187 85
BspEI T'CCGG_A
Cuts at: 2053 2391 5385 2053
Size: 338 2994 2390
Fragments arranged by size:
2994 2390 338
BspHI T'CATG_A
Cuts at: 3611 5341 3611
Size: 1730 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2332 2419 2820 3077 844
Size: 1488 87 401 257 3489
Fragments arranged by size:
3489 1488 401 257 87
BsrBI CCG'CTC
Cuts at: 1394 1823 3609 1394
Size: 429 1786 3507
Fragments arranged by size:
3507 1786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2079 4217 4391 65
Size: 1183 831 2138 174 1396
Fragments arranged by size:
2138 1396 1183 831 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2362 2448 4366 4553 1700
Size: 110 552 86 1918 187 2869
Fragments arranged by size:
2869 1918 552 187 110 86
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5722
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 2934 3782 4794 2934
Size: 848 1012 3862
Fragments arranged by size:
3862 1012 848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2153 2394 2679 2782 2881 3052 3419 3804
Size: 181 241 285 103 99 171 367 385
Cuts at: 3804 3821 5273 5359 5371 5717 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 285 241 181
171 103 99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2697 2697
Size: 5722
BtgI C'CryG_G
Cuts at: 513 1065 2004 2649 513
Size: 552 939 645 3586
Fragments arranged by size:
3586 939 645 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2752 487
Size: 683 56 1526 3457
Fragments arranged by size:
3457 1526 683 56
BtsI GCAGTG_nn'
Cuts at: 2248 3238 4032 4052 2248
Size: 990 794 20 3918
Fragments arranged by size:
3918 990 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 3169 4058
Size: 54 1425 50 275 18 243 1096 889
Cuts at: 4058 5687 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1096 889 275 243 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2339 2966 3652 1353
Size: 740 246 627 686 3423
Fragments arranged by size:
3423 740 686 627 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2435 2444 4309 4683 4829 387
Size: 1064 138 846 9 1865 374 146 1280
Fragments arranged by size:
1865 1280 1064 846 374 146 138 9
EcoICRI GAG'CTC
Cuts at: 2821 2821
Size: 5722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2585 2604 2648 2837 3785 4382
Size: 393 599 354 19 44 189 948 597
Cuts at: 4382 5169 5437 1239
Size: 787 268 1524
Fragments arranged by size:
1524 948 787 599 597 393 354 268
189 44 19
EcoRI G'AATT_C
Cuts at: 3031 3409 3031
Size: 378 5344
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2977 2122
Size: 855 4867
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 2221 2918 2959 4869 1678
Size: 543 697 41 1910 2531
Fragments arranged by size:
2531 1910 697 543 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2577 2609 4363 4395 4437
Size: 32 135 32 1005 32 1754 32 42
Cuts at: 4437 4469 1373
Size: 32 2626
Fragments arranged by size:
2626 1754 1005 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3066 3312 669
Size: 988 281 568 560 246 3079
Fragments arranged by size:
3079 988 568 560 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5722
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2588 2880 4836 5020 1305
Size: 1283 292 1956 184 2007
Fragments arranged by size:
2007 1956 1283 292 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3900
Fragments arranged by size:
3900 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2135 2771 3680 4039 4198 518
Size: 648 843 126 636 909 359 159 2042
Fragments arranged by size:
2042 909 843 648 636 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 2023 3138 3815 4963 5208 1606
Size: 417 1115 677 1148 245 2120
Fragments arranged by size:
2120 1148 1115 677 417 245
NaeI GCC'GGC
Cuts at: 2450 2450
Size: 5722
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4231
Fragments arranged by size:
4231 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NgoMIV G'CCGG_C
Cuts at: 2448 2448
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5722
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 3080 4625 1132
Size: 97 1851 1545 2229
Fragments arranged by size:
2229 1851 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2718 1108
Size: 1610 4112
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PstI C_TGCA'G
Cuts at: 830 3074 830
Size: 2244 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
PvuII CAG'CTG
Cuts at: 2023 2023
Size: 5722
SacI G_AGCT'C
Cuts at: 2823 2823
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SapI GCTCTTCn'nnn_
Cuts at: 2339 2339
Size: 5722
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 2998 3070 4205 4886 5077 826
Size: 219 1953 72 1135 681 191 1471
Fragments arranged by size:
1953 1471 1135 681 219 191 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3826 4727 4989 820
Size: 197 926 1883 901 262 1553
Fragments arranged by size:
1883 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5722
StyI C'CwwG_G
Cuts at: 513 1065 2004 3161 513
Size: 552 939 1157 3074
Fragments arranged by size:
3074 1157 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2279 2359 3749 3766 3919 4104 4549
Size: 1255 699 80 1390 17 153 185 445
Cuts at: 4549 325
Size: 1498
Fragments arranged by size:
1498 1390 1255 699 445 185 153 80
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3968 5680 96
Size: 274 80 33 51 1443 1991 1712 138
Fragments arranged by size:
1991 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5682 5714 5682
Size: 32 5690
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2284
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5722
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 2049 3851 2049
Size: 1802 3920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2389 3530 276
Size: 53 83 186 936 855 1141 2468
Fragments arranged by size:
2468 1141 936 855 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI