Restriction map for
pFN21AB8456
(Circular) (Six-base) MAPSORT of: pFN21AB8456.seq Check: 9507 from: 1 to: 6160
pFN21AB8456 6160 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3503 1937
Size: 1566 4594
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 2874 3042 3204 4223 5607 5875 1632
Size: 618 624 168 162 1019 1384 268 1917
Fragments arranged by size:
1917 1384 1019 624 618 268 168 162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 5059 6036 1203
Size: 3856 977 1327
Fragments arranged by size:
3856 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 3092 1166
Size: 1926 4234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2856 1184
Size: 1672 4488
AlwNI CAG_nnn'CTG
Cuts at: 2347 2451 3903 5475 2347
Size: 104 1452 1572 3032
Fragments arranged by size:
3032 1572 1452 104
ApaLI G'TGCA_C
Cuts at: 2927 4217 5373 2927
Size: 1290 1156 3714
Fragments arranged by size:
3714 1290 1156
ApoI r'AATT_y
Cuts at: 1493 2166 2223 3469 3695 3847 5956 1493
Size: 673 57 1246 226 152 2109 1697
Fragments arranged by size:
2109 1697 1246 673 226 152 57
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 3485 1870
Size: 73 1008 534 4545
Fragments arranged by size:
4545 1008 534 73
BamHI G'GATC_C
Cuts at: 2848 3490 3857 2848
Size: 642 367 5151
Fragments arranged by size:
5151 642 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2467 2947 3481 4936 618
Size: 325 217 627 680 480 534 1455 1842
Fragments arranged by size:
1842 1455 680 627 534 480 325 217
BanII G_rGCy'C
Cuts at: 1391 3479 1391
Size: 2088 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2313 928
Size: 971 414 4775
Fragments arranged by size:
4775 971 414
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4351 4385 1903
Size: 34 2414 34 3678
Fragments arranged by size:
3678 2414 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5262 4052
Size: 1210 4950
BclI T'GATC_A
Cuts at: 1548 3096 1548
Size: 1548 4612
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3419 4771 136
Size: 107 122 71 631 605 1747 1352 1525
Fragments arranged by size:
1747 1525 1352 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6155 6155
Size: 6160
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 2609 2931 2952 4221 5377 1790
Size: 819 322 21 1269 1156 2573
Fragments arranged by size:
2573 1269 1156 819 322 21
BmgBI CAC'GTC
Cuts at: 1318 2513 1318
Size: 1195 4965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3891 4849 442
Size: 416 53 83 399 24 2474 958 1753
Fragments arranged by size:
2474 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2781 1056
Size: 1725 4435
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2923 2923
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2253 2447 2582 2907 4820 1239
Size: 1014 194 135 325 1913 2579
Fragments arranged by size:
2579 1913 1014 325 194 135
Bpu10I CC'TnA_GC
Cuts at: 2384 2384
Size: 6160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2070 2642 4285 5150 5448 2070
Size: 572 1643 865 298 2782
Fragments arranged by size:
2782 1643 865 572 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2423 4823 882
Size: 617 924 2400 2219
Fragments arranged by size:
2400 2219 924 617
BsaAI yAC'GTr
Cuts at: 493 1204 2932 3951 493
Size: 711 1728 1019 2702
Fragments arranged by size:
2702 1728 1019 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3967 4349 4984
Size: 53 83 186 936 476 1958 382 635
Cuts at: 4984 275
Size: 1451
Fragments arranged by size:
1958 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2319 2349 1178
Size: 30 165 30 916 30 4989
Fragments arranged by size:
4989 916 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2645 2648 2899 3206 1186
Size: 883 576 3 251 307 4140
Fragments arranged by size:
4140 883 576 307 251 3
BseYI C'CCAG_C
Cuts at: 2601 2687 3028 3067 5363 5859 2601
Size: 86 341 39 2296 496 2902
Fragments arranged by size:
2902 2296 496 341 86 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5587
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3610 4371 5399 1540
Size: 275 186 1609 761 1028 2301
Fragments arranged by size:
2301 1609 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2931 4221 4306 5377 2931
Size: 1290 85 1071 3714
Fragments arranged by size:
3714 1290 1071 85
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2609 2931 2952 3479 4221 4306 5377
Size: 399 819 322 21 527 742 85 1071
Cuts at: 5377 1391
Size: 2174
Fragments arranged by size:
2174 1071 819 742 527 399 322 85
21
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3515 844
Size: 2671 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4047 1394
Size: 429 2224 3507
Fragments arranged by size:
3507 2224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2369 2674 2850 4655 4829 65
Size: 1183 1121 305 176 1805 174 1396
Fragments arranged by size:
1805 1396 1183 1121 305 176 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3341 4804 4991 1700
Size: 110 1531 1463 187 2869
Fragments arranged by size:
2869 1531 1463 187 110
BsrGI T'GTAC_A
Cuts at: 96 2420 96
Size: 2324 3836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6160
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstYI r'GATC_y
Cuts at: 1972 2848 3490 3857 4242 4259 5711 5797 5809
Size: 876 642 367 385 17 1452 86 12
Cuts at: 5809 6155 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 876 642 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4669
Fragments arranged by size:
4669 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 2534 2652 3051 3429 3676 4470 4490 2534
Size: 118 399 378 247 794 20 4204
Fragments arranged by size:
4204 794 399 378 247 118 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2709 5167 809
Size: 507 1393 2458 1802
Fragments arranged by size:
2458 1802 1393 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3426 3607 4496
Size: 54 1425 50 275 18 1596 181 889
Cuts at: 4496 6125 8
Size: 1629 43
Fragments arranged by size:
1629 1596 1425 889 275 181 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2020 2193 2888 2937 2958 3147 3252 3267
Size: 667 173 695 49 21 189 105 15
Cuts at: 3267 4090 1353
Size: 823 3423
Fragments arranged by size:
3423 823 695 667 189 173 105 49
21 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4747 5121 5267 387
Size: 1064 138 3158 374 146 1280
Fragments arranged by size:
3158 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2253 2447 2582 2874 2907 3042
Size: 393 618 3 194 135 292 33 135
Cuts at: 3042 3204 4223 4820 5607 5875 1239
Size: 162 1019 597 787 268 1524
Fragments arranged by size:
1524 1019 787 618 597 393 292 268
194 162 135 135 33 3
EcoO109I rG'GnC_Cy
Cuts at: 2819 2819
Size: 6160
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3032 3281 3032
Size: 249 5911
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 5307 1678
Size: 3629 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 3308 3340 3362
Size: 32 135 32 620 32 1084 32 22
Cuts at: 3362 3394 4801 4833 4875 4907 1373
Size: 32 1407 32 42 32 2626
Fragments arranged by size:
2626 1407 1084 620 135 42 32 32
32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3504 3750 669
Size: 988 281 1566 246 3079
Fragments arranged by size:
3079 1566 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2866 748
Size: 2118 4042
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2750 5274 5458 1305
Size: 1445 2524 184 2007
Fragments arranged by size:
2524 2007 1445 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3428 10
Size: 54 1425 343 1596 2742
Fragments arranged by size:
2742 1596 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3092 4118 4477 4636 518
Size: 648 1926 1026 359 159 2042
Fragments arranged by size:
2042 1926 1026 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2448 3364 3576 4253 5401 5646 1606
Size: 842 916 212 677 1148 245 2120
Fragments arranged by size:
2120 1148 916 842 677 245 212
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4669
Fragments arranged by size:
4669 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NheI G'CTAG_C
Cuts at: 1052 2777 1052
Size: 1725 4435
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 3518 5063 1132
Size: 97 2289 1545 2229
Fragments arranged by size:
2289 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6160
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2451 2742 1108
Size: 1343 291 4526
Fragments arranged by size:
4526 1343 291
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PmlI CAC'GTG
Cuts at: 2932 2932
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 4229 4261 5742 5774 2894
Size: 32 1303 32 1481 32 3280
Fragments arranged by size:
3280 1481 1303 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PstI C_TGCA'G
Cuts at: 830 2544 2659 3512 830
Size: 1714 115 853 3478
Fragments arranged by size:
3478 1714 853 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SapI GCTCTTCn'nnn_
Cuts at: 2193 3267 2193
Size: 1074 5086
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 2540 2655 3508 4643 5324 5515 826
Size: 219 1495 115 853 1135 681 191 1471
Fragments arranged by size:
1495 1471 1135 853 681 219 191 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 3341 1700
Size: 1641 4519
SmaI CCC'GGG
Cuts at: 1872 3487 1872
Size: 1615 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2085 2621 4264 5165 5427 820
Size: 197 926 142 536 1643 901 262 1553
Fragments arranged by size:
1643 1553 926 901 536 262 197 142
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3518 1132
Size: 2386 3774
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6160
StyI C'CwwG_G
Cuts at: 513 1065 2004 2122 3599 513
Size: 552 939 118 1477 3074
Fragments arranged by size:
3074 1477 939 552 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4187 4204 4357 4542 4987 325
Size: 1255 2607 17 153 185 445 1498
Fragments arranged by size:
2607 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2420 2580 4406
Size: 274 80 33 51 1443 443 160 1826
Cuts at: 4406 6118 96
Size: 1712 138
Fragments arranged by size:
1826 1712 1443 443 274 160 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 6120 6152 2319
Size: 32 3769 32 2327
Fragments arranged by size:
3769 2327 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2514 1316
Size: 1198 4962
XbaI T'CTAG_A
Cuts at: 3326 3496 3326
Size: 170 5990
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3064 3421 3064
Size: 357 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 3485 1870
Size: 1615 4545
XmnI GAAnn'nnTTC
Cuts at: 4289 4289
Size: 6160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI